Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_KO_pgi	1,275,721	C→T	E37K (GAA→AAA)	narL ←	response regulator in two‑component regulatory system with NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_KO_pgi	1,275,721	0	C	T	100.0%	149.2 / NA	43	E37K (GAA→AAA)	narL	response regulator in two‑component regulatory system with NarX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/21);  total (22/21)

CACAATGCGCCCTGAGAGGGACTTTTCGCGCAGTTTATCCAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTC  >  NC_000913_3_KO_pgi/1275578‑1275863                                                                                                                                                |                                                                                                                                               cacaATGCGCCCTGAGAGGGACTTTTCGCGCAGTTTATCCAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCaa                                                                                                                                           >  1:159598/1‑149 (MQ=255)               agagGGACTTTTCGCGCAGTTTAGCCAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCt                                                                                                                             <  1:72786/149‑1 (MQ=255)                  gggACTTTTCGCGCAGTTTTTCCAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGt                                                                                                                          <  1:19071/149‑1 (MQ=255)                            gcgcAGTTTATCCAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGa                                                                                                                <  2:458239/149‑1 (MQ=255)                              gcAGTTTATCTAGCGTTTCCAGCCCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATa                                                                                                              <  2:597104/149‑1 (MQ=255)                                   ttATCCAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTg                                                                                                         <  2:237068/149‑1 (MQ=255)                                         aGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTAc                                                                                                   >  2:345323/1‑149 (MQ=255)                                                    aCCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCAGCTCGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCgca                                                                                        <  1:169597/149‑1 (MQ=255)                                                                  gCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGt                                                                                                                          >  1:195413/1‑101 (MQ=255)                                                                  gCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGt                                                                                                                          <  2:195413/101‑1 (MQ=255)                                                                      atTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGGTATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGCTGATCGTCaa                                                                      >  1:114511/1‑149 (MQ=255)                                                                        tGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTACGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTTTTCTACGCCACGTCGCAGCACCCGGTGAGCGTccctc                                                                    >  2:316515/1‑145 (MQ=255)                                                                         gagaTCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATTGTCAATca                                                                   >  1:641302/1‑149 (MQ=255)                                                                             tCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATcagcag                                                               <  2:642359/149‑1 (MQ=255)                                                                               tAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGa                                                             <  1:345323/149‑1 (MQ=255)                                                                                aaCAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGAt                                                            >  1:370624/1‑149 (MQ=255)                                                                                 aCAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGAta                                                           <  1:647779/149‑1 (MQ=255)                                                                                  cAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCCTCAATCAGCAGGAtcg                                                          >  2:416174/1‑147 (MQ=255)                                                                                    ggAGCAGATCGGGATCAAGAGACTCCTCCAGTTCAATACCCTGTTCGCCATTAGTTGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTTATCGTCAATCAGCAGGAtagta                                                        <  1:333125/149‑1 (MQ=255)                                                                                      aTCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGc                                                      >  1:688892/1‑149 (MQ=255)                                                                                          gATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCCGTTCGCAGCATCTGTTGATCGTCAATCAGCAGGATAGTAGCCGGt                                                  >  1:135082/1‑149 (MQ=255)                                                                                           aTCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGtt                                                 >  1:116013/1‑149 (MQ=255)                                                                                           aTCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGtt                                                 >  2:492136/1‑149 (MQ=255)                                                                                           aTCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGtt                                                 >  1:210684/1‑149 (MQ=255)                                                                                               ggATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCt                                              >  1:387087/1‑148 (MQ=255)                                                                                               ggATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCt                                              <  2:387087/148‑1 (MQ=255)                                                                                                gATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGa                                            <  2:210684/149‑1 (MQ=255)                                                                                                 aTCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGAt                                           <  2:717839/149‑1 (MQ=255)                                                                                                  tCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACgg                                                                                                                                     <  2:401224/58‑1 (MQ=255)                                                                                                  tCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACgg                                                                                                                                     >  1:401224/1‑58 (MQ=255)                                                                                                     agagACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACt                                       >  1:591500/1‑149 (MQ=255)                                                                                                       agccTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCa                                     <  1:6308/146‑1 (MQ=255)                                                                                                        ggcTCCGCCAGGTCAATACCCTGTTCATTATGTGTCGCGTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCAt                                    <  1:460204/147‑1 (MQ=255)                                                                                                           tCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGt                                                                                                                                    >  2:110430/1‑50 (MQ=255)                                                                                                           tCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGt                                                                                                                                    <  1:110430/50‑1 (MQ=255)                                                                                                              gCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCAAGGGTAt                              >  1:423890/1‑149 (MQ=255)                                                                                                                    tCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGATTATTGGTTACTCATGGGTAtcttcgt                        <  2:343823/149‑5 (MQ=255)                                                                                                                      aaTACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTgg                      >  1:690530/1‑149 (MQ=255)                                                                                                                        tACCCTGTTCGCCATTACTTGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGAc                    <  1:182588/149‑1 (MQ=255)                                                                                                                                  gCCATTACTCGCTTTGCCAACCACGATGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCTTCATCGGGTGATCGTCTATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGaaa          <  1:533854/149‑1 (MQ=255)                                                                                                                                    cATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGt        >  1:72031/1‑149 (MQ=255)                                                                                                                                          tCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTc  >  1:519060/1‑149 (MQ=255)                                                                                                                                          tCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTc  >  2:626312/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                               CACAATGCGCCCTGAGAGGGACTTTTCGCGCAGTTTATCCAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTC  >  NC_000913_3_KO_pgi/1275578‑1275863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑