Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_KO_pgi	3,440,137	T→G	K298T (AAA→ACA)	rpoA ←	RNA polymerase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_KO_pgi	3,440,137	0	T	G	100.0%	117.9 / NA	36	K298T (AAA→ACA)	rpoA	RNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/18);  total (18/18)

TGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGA  >  NC_000913_3_KO_pgi/3439999‑3440280                                                                                                                                           |                                                                                                                                                tGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAg                                                                                                                                       <  1:385028/149‑1 (MQ=255)  gACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAACAGATGTTTTACCAAgg                                                                                                                                      >  1:660724/1‑149 (MQ=255)   aCCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCAAGGCTTTAATCTCAGTAAGAGATGTTTTACCAAgat                                                                                                                                     >  2:670111/1‑147 (MQ=255)            ttCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGttt                                                                                                                            >  1:628117/1‑149 (MQ=255)             tctcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAGGTTTTACCAAGGTTAGGCGtttt                                                                                                                           >  2:44016/1‑149 (MQ=255)                      aaCCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTc                                                                                                                  <  1:502705/149‑1 (MQ=255)                        ccTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCaa                                                                                                                <  2:163711/149‑1 (MQ=255)                        ccTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCCCaa                                                                                                                >  2:605526/1‑149 (MQ=255)                         cTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCTAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAAc                                                                                                               >  1:477619/1‑149 (MQ=255)                         cTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAtgttt                                                                                                                                              <  1:203215/118‑1 (MQ=255)                         cTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAtgttt                                                                                                                                              >  2:203215/1‑118 (MQ=255)                             acgtgtgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAACCTATCACGTCTATAATCTCAGTACGAGATGTGTTACTAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                                                                           <  1:386346/146‑1 (MQ=255)                                tgtgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTa                                                                                                        <  1:338406/149‑1 (MQ=255)                                              tCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATc                                                                                          >  1:324131/1‑149 (MQ=255)                                                     cGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGata                                                                                   >  2:571513/1‑149 (MQ=255)                                                         ggttGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGt                                                                               <  2:536116/147‑1 (MQ=255)                                                                  tGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCtt                                                                      >  2:692907/1‑149 (MQ=255)                                                                       aGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCt                                                                 >  2:669116/1‑149 (MQ=255)                                                                       aGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCt                                                                 >  1:471707/1‑149 (MQ=255)                                                                           ttCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTaa                                                             <  2:558272/149‑1 (MQ=255)                                                                            tCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCTAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAg                                                            <  1:599412/149‑1 (MQ=255)                                                                            tCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCACGGTACGCTGTACCAGATCACCGACATAGTGGATATCTTCTGCTTTAAg                                                            <  2:628117/149‑1 (MQ=255)                                                                            tCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAACTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGGTCAACCTCGGTACGCTGTACCAGATCACCGATATAGGGGATAGGTTCTGccatagt                                                            >  2:511180/1‑143 (MQ=255)                                                                                      tGCCGAGGGGCAGGCCACGGGAAGCCAGGGCGTCTTTGATCTCAGTAAGGGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATTACCGATATAGTGGATAGCTTTTGCTTTAAGGCAGTTAGCa                                                  <  2:359785/149‑1 (MQ=255)                                                                                      tGCCCAGAGACAGTGCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCa                                                  <  2:500065/149‑1 (MQ=255)                                                                                         ccAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCagag                                               >  1:247549/1‑149 (MQ=255)                                                                                         ccAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCagag                                               >  2:29183/1‑149 (MQ=255)                                                                                          cAGAGAAAGTCTACGACTAGCCAGCACGTCTTGAATCTCAGTAAGAGATGTTGTACCAAGGTTAGGCGTTTTAAGGAGCTCAATCTCGTTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGc                                              <  2:61925/149‑1 (MQ=255)                                                                                            gagaCAGTCCACGGGAAGCCAGCACGTCTTTACAAAATATATGAGATGTTTTACCAAGGGTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGAGGATAGCTTCTGCTTTAAGGCAGTTAGCCGAGCgg                                            >  2:340317/1‑149 (MQ=255)                                                                                             agaCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGa                                           >  1:650759/1‑149 (MQ=255)                                                                                                     cACGGGAATCGAGCATGTCTTTAATCTCGGTAAGGGATGTTTTACCAAGCTTAGGCATTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAAt                                   <  2:464686/149‑1 (MQ=255)                                                                                                            aGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTc                                                                     <  1:713487/108‑1 (MQ=255)                                                                                                            aGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTc                                                                     >  2:713487/1‑108 (MQ=255)                                                                                                            aGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGAt                            <  2:614674/149‑1 (MQ=255)                                                                                                             gCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATc                           <  1:571513/149‑1 (MQ=255)                                                                                                                      tCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAgg                  >  1:342432/1‑149 (MQ=255)                                                                                                                      tCTTTAATCGCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAgg                  <  2:406266/149‑1 (MQ=255)                                                                                                                                      gagaTGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAggatcgga  <  1:451842/149‑1 (MQ=255)                                                                                                                                           |                                                                                                                                                TGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGA  >  NC_000913_3_KO_pgi/3439999‑3440280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑