New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 22703944 (0.870)6 (0.120) 5/274 6.7 14.1% noncoding (1281/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
?NC_000913_3_KO_pgi = 4038805 31 (0.640)noncoding (1287/2905 nt) rrlA 23S ribosomal RNA of rrnA operon
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCG  >  NC_000913_3_KO_pgi/226891‑227129
                                                                                                                                                    |                                                                                          
aaCGGGGGCAAAACCTGCACCGGAGCTGCGGCAGCGACGTTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCCGAAGTGCGAATGCTGACATAAGTAACGATAAAGCggg                                                                                            <  1:533664/149‑1 (MQ=11)
 aCGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTCATGCGTTGTTGGGTAGGGGAGCGTTATGTAATCCTGTGAAGGAGTACTGTTATTTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGt                                                                                           <  1:665420/149‑1 (MQ=11)
    gggCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGaa                                                                                        >  2:282139/1‑149 (MQ=37)
     ggCTAAACCATGCACCGAAGTTGCGGCAGCGGCGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGaaa                                                                                       <  2:65408/149‑1 (MQ=255)
      gcgaaaCCATGCCCCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGGGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGaaaa                                                                                      <  1:239789/146‑1 (MQ=255)
      gCTAAACCATGCACCGGAGCGGCGGCAGCGACGGTTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTCGACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATCAGGAACGTTAAAGCGGGTGaaaa                                                                                      <  2:740294/149‑1 (MQ=11)
        tAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGc                                                                                    >  1:760085/1‑149 (MQ=38)
          aaCCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAACCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGCAGTGAGAATGCTGGCATAAGTAACGATAAAGCGGGTGAAAAGccc                                                                                  >  1:210416/1‑149 (MQ=255)
           aCCATGCACCGAAGCTGCGGCATCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCg                                                                                 <  1:443047/149‑1 (MQ=37)
              aTGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGGGAAAAGCCCGCTc                                                                              >  2:674885/1‑149 (MQ=37)
              aTGCACCGAAGCTGCGGCAGCGACGCTTATGCGGTGTTGGGGAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGGATGCTGGAGGTATCAGAAGTGCGAATGCTGAGATAAGTAACGATAAAGCGGGAGAAATGCCCGCTc                                                                              >  1:290557/1‑149 (MQ=11)
               tGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGGTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCg                                                                             >  2:781056/1‑149 (MQ=38)
                  aCCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCg                                                                          >  1:506918/1‑149 (MQ=38)
                     gAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGATATGAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGaa                                                                       <  1:437810/149‑1 (MQ=255)
                     gAAGCTGCGGCAGCGACGCTTATGAGTTGTTGGGTAGCGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGCGAGGTATGCTGGAGGTATCAGATGTGCGAATGCTGACATAAGTAACGATAAAGCTGGTGCAAAGCCCGCTCGCCGGaa                                                                       <  1:835808/149‑1 (MQ=14)
                           ggggCAGCGGCGCTGATGCGTTGTTGGGGAGGGGGGCGTTCGGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCaa                                                                 <  2:354611/147‑1 (MQ=11)
                             ggCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAgg                                                               >  1:327113/1‑149 (MQ=38)
                                 gCGTCGCTCATGTGTTGTGGGGTAGGGGAGCGGTCTGTAAGCCGGTGAAGGTGTACTGTGAGGTATGCGGGAGGTATCAGAAGTGCGAATGCTGACATAAGCAACGATAAAGCGGGTGAAAAGCCCTCTCGCCGGAAGACCAACGGTTc                                                           <  2:269513/149‑1 (MQ=11)
                                 gCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTc                                                           <  1:268676/149‑1 (MQ=38)
                                 gCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTc                                                           >  2:699315/1‑149 (MQ=38)
                                       cTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTcc                                                     >  1:240529/1‑149 (MQ=38)
                                                  ttgGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACAGAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGACCAACGTTAAtac                                          >  2:526627/1‑147 (MQ=255)
                                                    gggTAGGGGAGCGTGCTGTAAGCCTGTGAAGGTGTACTGTGAGATATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGCTCCTGTCCAACGTTAATCggg                                        <  2:797930/149‑1 (MQ=255)
                                                     ggTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTATCCAATGGTAATCgggg                                       >  1:567466/1‑149 (MQ=255)
                                                        aGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAg                                    <  1:766768/149‑1 (MQ=35)
                                                            gAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTg                                >  2:264476/1‑149 (MQ=35)
                                                               cGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGTGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGt                             >  2:196402/1‑149 (MQ=25)
                                                                 ttCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCg                           >  1:32870/1‑149 (MQ=35)
                                                                  tCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCaa                                                   <  1:736304/124‑1 (MQ=37)
                                                                  tCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCaa                                                   >  2:736304/1‑124 (MQ=37)
                                                                   gtggaaGCCTGGGAAGGTGTACTGGGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCGGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCGCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGAc                         <  2:287735/145‑1 (MQ=11)
                                                                    tGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGACGAcc                        >  1:652285/1‑149 (MQ=25)
                                                                     gTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGAccc                       <  2:357462/149‑1 (MQ=35)
                                                                          ccTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAg                  <  1:596597/149‑1 (MQ=34)
                                                                          ccTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAg                  >  2:598723/1‑149 (MQ=35)
                                                                          ccTGTGAAGGTGTACTGCGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAg                  <  1:645986/149‑1 (MQ=25)
                                                                           cTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAgg                 >  2:431608/1‑149 (MQ=35)
                                                                            tgtgAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGc                >  1:82398/1‑149 (MQ=35)
                                                                               gAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAg             <  1:280676/149‑1 (MQ=34)
                                                                                   gtgtACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACATTAATCGGGGCAGGGTGAGTCGACACCTAAGGCGGGGCCg         >  1:801261/1‑149 (MQ=255)
                                                                                     gtACTGTGAGGTATGCTGGAGATATCAGAAGTGCGAATGCTGACATAAGTAAGGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTGAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGCGGCCGaa       >  2:435504/1‑149 (MQ=255)
                                                                                        cTGTGAGGTATGCTGGAGGTATCAGAAGTGACAATCAGGACAGAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAgg    >  1:669855/1‑149 (MQ=11)
                                                                                         tgtgAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTc                                                                              <  1:899325/74‑1 (MQ=35)
                                                                                         tgtgAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTc                                                                              >  2:899325/1‑74 (MQ=35)
                                                                                          gtgAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCg  >  2:614582/1‑149 (MQ=32)
                                                                                                                                                    |                                                                                          
AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCG  >  NC_000913_3_KO_pgi/226891‑227129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.