New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	496998 =	NA (NA)	7 (0.150)	6/260	5.9	NA	intergenic (+4/‑177)	htpG/adk	protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase
	?	NC_000913_3_KO_pgi	497030 =	NA (NA)					intergenic (+36/‑145)	htpG/adk	protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase
Rejected: Coverage evenness skew score above cutoff.

CGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAA  >  NC_000913_3_KO_pgi/496858‑497145                                                                                                                                             |                                                                                                                                                    cGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGa                                                                                                                                             <  2:383849/149‑1 (MQ=255)    cAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGAAAGCTGGAAGATCGGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGa                                                                                                                                          >  2:548804/1‑149 (MQ=255)            gAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGACGCTGCGGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAAACAGCTGCTGGTTGCCTGATGTGATGCCGGATGACCTTCttg                                                                                                                                  >  1:184620/1‑149 (MQ=255)                aTGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCAt                                                                                                                              >  2:583320/1‑149 (MQ=255)                  gAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATcc                                                                                                                            >  1:902532/1‑149 (MQ=255)                     gCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAAt                                                                                                                                                   <  1:780599/123‑1 (MQ=255)                     gCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAAt                                                                                                                                                   >  2:780599/1‑123 (MQ=255)                     gCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGcc                                                                                                                                                >  2:886298/1‑126 (MQ=255)                     gCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGcc                                                                                                                                                <  1:886298/126‑1 (MQ=255)                      cGAAGTGCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCa                                                                                                                        <  2:810879/149‑1 (MQ=255)                       gAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTCCTGGTTTGCTGATGTAGTGCCGGATGCCCTTCCTGTCATCCGGcct                                                                                                                       >  1:869303/1‑147 (MQ=255)                        aaGTTCAGCGAGTGGGTGGAACTGCTGCTGGATCAGGCGCGGCTGGCAGAACGCGGAACGCTGGAAGATCCCAACCTGTTTATTCGTCATATGAACCAGCAGCTGGGCTCCTGATGTGATGCACGCTGACCTTCCTGTCATccagaagt                                                                                                                      >  2:436944/1‑143 (MQ=255)                              aGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTTGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGCAGCAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATttttctt                                                                                                                <  1:26997/149‑1 (MQ=255)                              aGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATttttctt                                                                                                                >  2:30013/1‑149 (MQ=255)                                       gTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCatcatc                                                                                                       >  1:178990/1‑149 (MQ=255)                                       gTAGAACTGCTGCTGGATCAGGCACTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAAACTGTTTATTCGTCGTATGAACCCGCTGCTGGTTTCCTGATGTACTGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCatcatc                                                                                                       >  2:800352/1‑149 (MQ=255)                                        tAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCt                                                                                                      <  1:106053/149‑1 (MQ=255)                                          gAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGc                                                                                                    <  2:732970/149‑1 (MQ=255)                                                   ctgGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGGCCTTCGTGTCATCCGGCATTTTTCTTTTCCTAATCTGCACTTTCCGc                                                                                           >  1:137791/1‑149 (MQ=255)                                                                       aGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGGGTCATCCGGCATTTTTCTTTTCATCATCTGCCCTTTCCACAAATTATCTCGCCATTAccc                                                                       >  2:299754/1‑149 (MQ=255)                                                                            gcgGCACGGTTGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTc                                                                  <  1:548745/149‑1 (MQ=255)                                                                                        aaGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAACTTATCTCGCCATTAACCGTTTACGCCCCGGGtcc                                                      >  2:496005/1‑147 (MQ=255)                                                                                               gAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCtt                                               <  1:198838/149‑1 (MQ=255)                                                                                                  ccTGTTTCTTCGGCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCAGCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAg                                            <  2:443706/149‑1 (MQ=255)                                                                                                        tATTCGCCGTATCAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATc                                      <  1:104397/149‑1 (MQ=255)                                                                                                             gtcgtATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTg                                 >  1:725230/1‑149 (MQ=255)                                                                                                              tcgtATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCCATCGCCTGt                                >  1:420359/1‑149 (MQ=255)                                                                                                                  aTGAACTCGCTGCCGGTTCCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTGTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTtggt                            <  1:259691/149‑1 (MQ=255)                                                                                                                  aTGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAg                                                                <  2:902120/113‑1 (MQ=255)                                                                                                                  aTGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAg                                                                >  1:902120/1‑113 (MQ=255)                                                                                                                  aTGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTATGGCGTCAATAGccgtttgtt                            >  2:140361/1‑142 (MQ=255)                                                                                                                        gcgtcgctgGTTTCCTGATGTAATTCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATc                      <  2:28022/144‑1 (MQ=255)                                                                                                                          gttgctgTTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGCCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCAACATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGt                    <  2:82174/147‑1 (MQ=255)                                                                                                                          gctgctGGTTTCCTGATGTCATGCCGGATGACCTTCGTGTCATCCGGCATGTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGt                    <  1:583320/149‑1 (MQ=255)                                                                                                                                 gTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGc             >  1:127055/1‑149 (MQ=255)                                                                                                                                 gTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTAACGc             >  1:96222/1‑149 (MQ=255)                                                                                                                                    tCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATTCTCTCTTCCACCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttt          <  1:433612/149‑1 (MQ=255)                                                                                                                                     ccTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCtttt         <  1:30013/149‑1 (MQ=255)                                                                                                                                      cTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttt        >  1:544211/1‑149 (MQ=255)                                                                                                                                       tGATGTAATGCCGGATGACGTTCGTGTCATCCGGCATTTTTCTTTTCATCCTCTGAACTTTCCGCCAATTATCTAGCCATTAACCGTTTCAGCCCCAGGTGCCTTTATTGAGGCAATAGCCTGTTGGAGGTATCGTTCATTGCTTtctc       >  1:183566/1‑149 (MQ=255)                                                                                                                                       tGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCCTCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTc       >  1:8821/1‑149 (MQ=255)                                                                                                                                       tGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTc       >  1:875326/1‑149 (MQ=255)                                                                                                                                         aTGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttga     >  1:74491/1‑146 (MQ=255)                                                                                                                                            tAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTGTTTCaaaaa  >  1:473064/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                                    CGGCAGATACTGAAGATGAAGCGAAGTTCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGATGACCTTCGTGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAA  >  NC_000913_3_KO_pgi/496858‑497145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.