New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 227039	44 (0.870)	6 (0.120)	5/274	6.7	14.1%	noncoding (1281/2904 nt)	rrlH	23S ribosomal RNA of rrnH operon
	?	NC_000913_3_KO_pgi	= 4038805	31 (0.640)					noncoding (1287/2905 nt)	rrlA	23S ribosomal RNA of rrnA operon
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTAAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAA  >  NC_000913_3_KO_pgi/4038658‑4038871                                                                                                                                                    |                                                                   cTAAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAAAGGGTGAAAAGc                                                                   >  1:244613/1‑149 (MQ=255)  tAAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGcc                                                                  <  1:334191/149‑1 (MQ=40)    aaCCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCg                                                                <  2:367986/149‑1 (MQ=255)        aTGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCg                                                            >  1:69495/1‑149 (MQ=255)        aTGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTACTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAATGATAAAGCGGGGGAAAAGcccgcccg                                                            >  2:881504/1‑149 (MQ=255)          gCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAAGGATGACAACAGTAACGATGAAGGGGGGGAAAAGCACGCTCgac                                                          >  1:205415/1‑147 (MQ=14)             ccGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCgat                                                       >  2:903510/1‑147 (MQ=255)              cGAAGCTGCGGCAGCGACACTATGTGTTGTCGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGAATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCACGCCGGaa                                                      >  1:474791/1‑149 (MQ=255)                  gCTGCGGCAGCGACACTATATGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGCAGGTATCAGAAGTGCGAATGCTGAGATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGAcc                                                  >  2:764035/1‑149 (MQ=255)                      cGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCCTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAgg                                              >  2:791684/1‑149 (MQ=255)                          aGCGACACTATGTGTTGGTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTc                                          <  1:744947/149‑1 (MQ=255)                           gCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAGGGTGTGCTGTGAGGCGTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCTCGCTCGCCGGAAGCCCAGGGGTTcc                                         >  2:215382/1‑149 (MQ=14)                              acacTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGt                                      <  1:715393/149‑1 (MQ=38)                              acacGATGTGTTGGTGGGTAGGGGAGCGTTCTGCAAGCCTGAGAAGGTGTGTTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTACCGATAAAGCGAGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGt                                      <  2:274954/149‑1 (MQ=11)                              aacctttgtgATGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAACGCCCGCTCGCCGGAAGACCAAGGGTTCCTGt                                      <  2:721454/143‑1 (MQ=255)                                   aTGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAAc                                 <  2:85532/149‑1 (MQ=35)                                      ggttgttGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATACAGCGGATGACACGCCGGGTCGTCTGAACACCAAGGGTTCCTGTCCAACGtt                              >  2:412353/2‑149 (MQ=11)                                        ttgttgGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTaa                            <  2:69495/149‑1 (MQ=32)                                           ttgGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTG‑AAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCgg                        >  2:709027/1‑149 (MQ=17)                                            ggggTAGGGGAGCGCTCTGTAAGCCTGTGAAGGGGTGCTTTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACACACGTAACGATAAAGCGGGTGGAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCgg                        <  2:393316/148‑1 (MQ=11)                                              ggTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCgggg                      <  1:75945/149‑1 (MQ=17)                                                 aGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAg                   >  1:870443/1‑149 (MQ=32)                                                   gggAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGTGGGAGCAGAAGTGCGAATGCTGACATAGGTAACAATAACGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGCTCCTGCCCAACCTCAATCGGGGCAggc                 >  2:52745/1‑148 (MQ=11)                                                    tgAGCGTTTGGTAAGCCTGTGAAGGTGTGCTGGGAGGGATGCGGGAGGTATCAGAAGTGGTAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCGCGCCGGAAGACCAAGGGTTCCTGTCCATCGTTAATCGGTGCAGGGt                <  2:215135/148‑1 (MQ=11)                                                    ggAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGCGAGCACACTTTCATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGt                <  1:663158/149‑1 (MQ=11)                                                       gCGTTCTGTAAGCCTTTGAAGGTGTGCTGTGTAGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGCCTTCTTGTCCAACGTTAATCGGGGCAGGGTGAg             <  2:56794/149‑1 (MQ=11)                                                       gCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGTCGGGtaat             <  2:791448/149‑4 (MQ=12)                                                           tCTGTAACCCTGTGAACGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGGCCAACAATCTGGCCCCAACGTTAATCGGGGCAGGGTGAGTCGa         <  2:647257/149‑1 (MQ=11)                                                             tGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGAcc       >  1:743768/1‑149 (MQ=32)                                                              gTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAgg                  >  2:504924/1‑137 (MQ=33)                                                              gTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAgg                  <  1:504924/137‑1 (MQ=32)                                                                aaGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCt    <  2:281147/149‑1 (MQ=32)                                                                aaGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCt    >  1:809192/1‑149 (MQ=32)                                                                  gCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTACTCGGGACAGGGTGAGTCGTCCCCtca  >  2:5452/1‑147 (MQ=255)                                                                                                                                                    |                                                                   CTAAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAA  >  NC_000913_3_KO_pgi/4038658‑4038871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.