Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_KO_pgi 1,978,503 Δ776 bp insB1insA insB1, insA

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_KO_pgi 1978503 1979278 776 48 [0] [0] 49 insB1–insA insB1,insA

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 19785020 (0.000)47 (0.980) 40/270 0.2 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913_3_KO_pgi 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein

GATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAA  >  NC_000913_3_KO_pgi/1979134‑1979427
                                                                                                                                                 |                                                                                                                                                     
aaggggtcttgagactgatttaaaggttttccgaaatggacgcaactgtactcgtaactacacgcacatacaacggaggggggctgcgattttcaataatgcgttatgcagatcacacaaaacactcaattacttaacataaatgtata                                                                                                                                                    <  2:703933‑M2/4‑1 (MQ=255)
 atgtgtctttccacttatctaataattttcctaaatcgacgcaactgtacttgtcactacacgcatatacaacggaggggtgctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATaa                                                                                                                                                   <  2:223157‑M2/5‑1 (MQ=255)
     gtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCa                                                                                                                                               >  1:301377‑M2/141‑149 (MQ=255)
      tctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCAt                                                                                                                                              <  1:71012‑M2/10‑1 (MQ=255)
      tctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCAt                                                                                                                                              >  1:25739‑M2/140‑149 (MQ=255)
              cttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgt                                                                                                                                      <  2:853764‑M2/18‑1 (MQ=255)
                tatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgttt                                                                                                                                    <  2:746467‑M2/20‑1 (MQ=255)
                   ctaagatttttcctaaatcgacgcaattgtactcgttactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                                 <  1:562927‑M2/23‑1 (MQ=255)
                   ctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                                 <  1:483341‑M2/23‑1 (MQ=255)
                    taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagctcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTGGTTTTggg                                                                                                                                >  1:859017‑M2/126‑149 (MQ=255)
                    taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTcgg                                                                                                                                >  1:417133‑M2/126‑149 (MQ=255)
                    taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTCTTggg                                                                                                                                >  2:649694‑M2/126‑149 (MQ=255)
                    taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggcgctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATCCTTTTTATTTggg                                                                                                                                >  2:80307‑M2/126‑149 (MQ=255)
                     aagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACtt                                                                                                                                          >  2:83332‑M2/125‑138 (MQ=255)
                     aagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACtt                                                                                                                                          <  1:83332‑M2/14‑1 (MQ=255)
                     aagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacaatgaattacttaacataaatgTATAAGTCATACTTTTGTCTtgggg                                                                                                                               >  2:800236‑M2/125‑148 (MQ=255)
                        atttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttccataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTa                                                                                                                            >  1:234126‑M2/122‑149 (MQ=255)
                        atttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTa                                                                                                                            <  2:13060‑M2/28‑1 (MQ=255)
                        atttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctacgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGATGTa                                                                                                                            >  1:764515‑M2/122‑149 (MQ=255)
                               ctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAt                                                                                                                     >  2:89288‑M2/115‑149 (MQ=255)
                               ctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAt                                                                                                                     <  1:373048‑M2/35‑1 (MQ=255)
                                    tcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGtt                                                                                                                >  1:488807‑M2/110‑149 (MQ=255)
                                           aactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTGAAAAAGt                                                                                                         >  1:546209‑M2/103‑149 (MQ=255)
                                                  ctcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTGTTGGGTGTATTTCTATCTGTTAAAAAGTTTttcat                                                                                                  >  2:859710‑M2/96‑147 (MQ=255)
                                                     gtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctac                                                                                               <  1:649757‑M2/57‑1 (MQ=255)
                                                       cactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacgc                                                                                             <  2:764515‑M2/59‑1 (MQ=255)
                                                        actacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatgacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAACTGTTAAAAAGTTTTTcgctacgct                                                                                           >  2:21724‑M2/90‑149 (MQ=255)
                                                          tacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGttttt                                                                                                     >  1:874404‑M2/88‑138 (MQ=255)
                                                          tacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTGTTGGGTGTATTTCAATCTGTTAAAAAGttttt                                                                                                     <  2:874404‑M2/51‑1 (MQ=255)
                                                               gcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaagc                                                                                    >  1:103935‑M2/83‑149 (MQ=255)
                                                                        acggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTTTTTTTGTTGTATTTCAATCTGTTCAAAAGTTTTTCGCTACGCTAGCAGACACaaatcac                                                                           >  2:569364‑M2/74‑146 (MQ=255)
                                                                         cggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGaaa                                                                          <  1:664586‑M2/77‑1 (MQ=255)
                                                                                 gggttcgattttgaataatgcgtgattcagatcacacaaaacaatcaattagttaacataaatgTATAAGTCATACTTCTGGTTTGGGTGTATTTCAATCTGTTAAGAAGTTGTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaata                                                                  <  2:859017‑M2/85‑1 (MQ=255)
                                                                                  gctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaataa                                                                 >  2:862480‑M2/64‑149 (MQ=255)
                                                                                           ttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAATAATGAAACAGGAATAATCGAAATgg                                                        >  1:894592‑M2/55‑149 (MQ=255)
                                                                                                    gcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTgcg                                               <  1:363336‑M2/104‑1 (MQ=255)
                                                                                                    gcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTgcg                                               <  1:361506‑M2/104‑1 (MQ=255)
                                                                                                               atcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCaaaa                                    <  1:562642‑M2/115‑1 (MQ=255)
                                                                                                                tcacaaaaaacactcaagtaattaacataaaggTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGTAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAAt                                   <  2:181527‑M2/116‑1 (MQ=255)
                                                                                                                  acacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATaa                                 >  1:547753‑M2/32‑149 (MQ=255)
                                                                                                                    acaaaacactcaattacttaacataaataTATAAGTAATACTTTTGTATTGGGTGTATTTCAATCTTTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGATATTGGGTGTTGCGCACAGCCAACATAacc                               >  2:104893‑M2/30‑148 (MQ=255)
                                                                                                                     caaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCTAAATGGGATGTTGCGCACAGTCAAAATAACTc                              >  2:70960‑M2/29‑149 (MQ=255)
                                                                                                                      aaaacactcaattacttaacataaatgTATAAGTCATCCTTTTGTTTTGAGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTAAGCATTCAAGCTAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCa                             <  1:757160‑M2/122‑1 (MQ=255)
                                                                                                                       aaacactcaattacttaacataaatgTATAAGTCACACTTGTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCAc                            <  1:244739‑M2/123‑1 (MQ=255)
                                                                                                                        aacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACCCAGCGCCAGAGAAATGAAACAGGAATAATTGAAATGGGATGTTGCGCACAGTCAAAAtaactaccc                           >  1:662492‑M2/26‑145 (MQ=255)
                                                                                                                          cactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGt                         >  1:258445‑M2/24‑149 (MQ=255)
                                                                                                                             tcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTCAAAAGTTTTTCGCTACGCTAGCAAGCAAAACTCAAACAGGACTAATCCAAAAGCGATGTTGCGCACACTCAAAATAACTCACCGTaaa                      >  2:31092‑M2/21‑149 (MQ=255)
                                                                                                                                              atgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTataaa     >  1:226447‑M2/4‑149 (MQ=255)
                                                                                                                                                 tataAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTataaataa  >  1:533216/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAA  >  NC_000913_3_KO_pgi/1979134‑1979427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.