Note: the reference genome BOP27 is a known variant of E. coli K12 MG1655 E. coli K12 MG1655.

ALE, Flask, Isolate, Technical Replicate Clonal or Population ALE Sample Details Base Media Carbon Source(g/L) Nitrogen Source(g/L) Phosphorus Source(g/L) Sulfur Source(g/L) Calcium Source(g/L) Supplement(g/L) Temperature (Celsius) Strain Additional Strain Details Taxonomy ID Reference Genome
A1 F250 I1 R1 clonal None M9 None None None None None None 37 679895 E. coli BW25113 delta-pfkA delta-pfkB delta-zwf None ['/var/data/addgene131706.gb', ['/var/data/KX077536.1.gb', '/var/data/CP009273.1.gb']]
A1 F250 I2 R1 clonal None M9 None None None None None None 37 679895 E. coli BW25113 delta-pfkA delta-pfkB delta-zwf None ['/var/data/addgene131706.gb', ['/var/data/KX077536.1.gb', '/var/data/CP009273.1.gb']]
A1 F252 I1 R1 clonal None M9 None None None None None None 37 679895 E. coli BW25113 delta-pfkA delta-pfkB delta-zwf None ['/var/data/addgene131706.gb', ['/var/data/KX077536.1.gb', '/var/data/CP009273.1.gb']]
A1 F400 I3 R1 clonal None M9 sodium formate(6) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) chloramphenicol(30 mg/L),abx, 37 679895 E. coli BW25113 delta-pfkA delta-pfkB delta-zwf Illumina,Kapa/ 13 ['/var/data/addgene131706.gb', ['/var/data/KX077536.1.gb', '/var/data/CP009273.1.gb']]
A1 F400 I4 R1 clonal None M9 sodium formate(6) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) chloramphenicol(30 mg/L),abx, 37 679895 E. coli BW25113 delta-pfkA delta-pfkB delta-zwf None ['/var/data/addgene131706.gb', ['/var/data/KX077536.1.gb', '/var/data/CP009273.1.gb']]
A1 F400 I5 R1 clonal None M9 sodium formate(6) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) chloramphenicol(30 mg/L),abx, 37 679895 E. coli BW25113 delta-pfkA delta-pfkB delta-zwf None ['/var/data/addgene131706.gb', ['/var/data/KX077536.1.gb', '/var/data/CP009273.1.gb']]