Notes

Publication:

10.1101/634105

10.1016/j.ymben.2023.01.012

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
0 1 1
1 1 3
2 1 3
3 1 3
4 1 3
5 1 3
6 1 3
7 1 3
8 1 3
9 9 25

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 53 83
Multiple Base Substitutions 0 0
Deletions 21 65
Insertions 8 8
Mobile Element Insertions 12 18
Amplifications 0 0
Gene Conversions 0 0
Inversions 0 0
Total 94 174

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4 2 422.4 11956043 98.9% 11824526 164.0
A1 F50 I1 R1 11 4 40.2 1256204 98.6% 1238617 150.4
A1 F50 I2 R1 10 4 70.5 2212154 98.5% 2178971 150.3
A1 F50 I3 R1 13 5 45.9 1429097 99.0% 1414806 150.4
A2 F50 I1 R1 11 4 44.7 1400463 98.4% 1378055 150.4
A2 F50 I2 R1 9 3 49.7 1558184 98.4% 1533253 150.3
A2 F50 I3 R2 11 4 68.6 2138883 99.0% 2117494 150.4
A3 F50 I1 R1 8 3 54.1 1685985 99.0% 1669125 150.4
A3 F50 I2 R2 8 3 65.0 2026404 99.0% 2006139 150.4
A3 F50 I3 R1 12 4 43.4 1375603 97.2% 1337086 150.3
A4 F50 I1 R1 9 4 141.3 4397970 99.2% 4362786 150.4
A4 F50 I2 R2 12 4 66.8 2112788 97.7% 2064193 150.3
A4 F50 I3 R1 10 3 45.8 1429983 98.8% 1412823 150.4
A5 F50 I1 R1 10 3 89.2 2790407 98.5% 2748550 150.4
A5 F50 I2 R1 10 3 66.7 2080639 98.8% 2055671 150.4
A5 F50 I3 R2 10 3 66.6 2073581 99.0% 2052845 150.4
A6 F50 I1 R1 10 5 61.6 1928186 98.5% 1899263 150.4
A6 F50 I2 R2 11 6 64.0 1996206 99.0% 1976243 150.3
A6 F50 I3 R1 11 4 87.2 2754937 97.9% 2697083 150.2
A7 F50 I1 R2 10 5 64.8 2025369 98.7% 1999039 150.4
A7 F50 I2 R1 9 3 103.4 3292674 97.1% 3197186 150.2
A7 F50 I3 R2 29 4 68.9 2138251 99.3% 2123283 150.4
A8 F50 I1 R2 10 4 73.9 2314347 98.5% 2279631 150.3
A8 F50 I2 R1 11 3 45.9 1441819 98.4% 1418749 150.3
A8 F50 I3 R1 14 4 46.7 1462666 98.9% 1446576 150.4

Mutation Needle Plot

Mutation Targets

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