breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 443,040 C→T intergenic (+43/+11) thiI → / ← yajL tRNA s(4)U8 sulfurtransferase/oxidative‑stress‑resistance chaperone
RA 660,573 T→C N269S (AAC→AGC)  ybeF ← LysR family putative transcriptional regulator
RA 701,381 (C)5→4 coding (214/1221 nt) nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
RA 874,067 G→A R363R (CGG→CGA yliE → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 1,029,739 (A)8→7 coding (231/1191 nt) rlmI ← 23S rRNA m(5)C1962 methyltransferase, SAM‑dependent
RA 1,187,352 G→A L297L (CTG→TTG)  roxA ← 50S ribosomal protein L16 arginine hydroxylase; 2‑oxoglutarate oxygenase
RA 1,728,884 G→A A180T (GCG→ACG)  rnt → RNase T; exoribonuclease T; structured DNA 3' exonuclease; RNA processing; DNA repair
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,262,665 G→A S277S (AGC→AGT fruB ← fused fructose‑specific PTS enzymes: IIA component/HPr component
RA 2,465,722 Δ1 bp coding (422/933 nt) yfdC → putative inner membrane protein
RA 2,725,672 (C)5→4 coding (75/1299 nt) kgtP ← alpha‑ketoglutarate transporter
RA 3,156,603 T→C intergenic (+85/‑20) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
RA 3,263,200 G→A A154V (GCT→GTT)  tdcD ← propionate kinase/acetate kinase C, anaerobic
RA 3,378,539 C→G G38A (GGT→GCT)  rplM ← 50S ribosomal subunit protein L13
RA 3,485,967 A→G intergenic (‑149/‑153) yhfA ← / → crp OsmC family protein/cAMP‑activated global transcription factor, mediator of catabolite repression
RA 3,510,180 T→C N266S (AAC→AGC)  yhfX ← putative pyridoxal 5'‑phosphate binding protein
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,634,152 T→C pseudogene (307/1194 nt) yhiL ← pseudogene, DUF4049 family protein
RA 3,779,384 A→G S111G (AGC→GGC)  lldR → dual role activator/repressor for lldPRD operon
RA 3,823,724 C→T T442I (ACC→ATC)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,931,183 (G)7→8 intergenic (‑90/‑133) ravA ← / → kup hexameric AAA+ MoxR family ATPase, putative molecular chaperone/potassium transporter
RA 3,943,892 (G)5→4 noncoding (189/2904 nt) rrlC → 23S ribosomal RNA of rrnC operon
RA 3,999,387 Δ1 bp coding (1405/2163 nt) uvrD → DNA‑dependent ATPase I and helicase II
RA 4,037,513 T→C intergenic (+178/‑6) alaT → / → rrlA tRNA‑Ala/23S ribosomal RNA of rrnA operon
RA 4,219,696 C→T G367G (GGC→GGT aceK → isocitrate dehydrogenase kinase/phosphatase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,607,712 C→T intergenic (‑12/‑91) rsmC ← / → holD 16S rRNA m(2)G1207 methyltransferase, SAM‑dependent/DNA polymerase III, psi subunit

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 734301–734433 734766–734475 43–466 36 [35] [34] 36 rhsO pseudogene, Rhs family
* * ÷ NC_000913 3423718–3424234 3424579–3424238 5–862 36 [35] [33] 36 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)75 (1.130) 60/290 0.2 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein