Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,779,211 G→C 5.6% P31A (CCA→GCA)  ydiP ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,779,2110GC 5.6% 227.6 / 2.6 69P31A (CCA→GCA) ydiPputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (37/27);  new base C (2/2);  total (40/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGCAGCCTGCCGCGTTCTATCACCACAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCG  >  NC_000913/1779064‑1779359
                                                                                                                                                   |                                                                                                                                                    
cATGCAGCCTGCCGCGTTCTATCACCACAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGa                                                                                                                                                     <  1:193689/149‑1 (MQ=255)
 aTGCAGCCTGCCGCGTTCTATCACCACAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCTATTTTGGat                                                                                                                                                    <  4:156388/149‑1 (MQ=255)
 aTGCAGCCTGCCGCGTTCTATCACCACAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGat                                                                                                                                                    >  8:266002/1‑149 (MQ=255)
  tGCAGCCTGCCGCGTTCTATCACCACAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGatc                                                                                                                                                   <  2:50748/149‑1 (MQ=255)
    cAGCCTGCCGCGTTCTATCACCACAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGatcat                                                                                                                                                 <  3:148806/149‑1 (MQ=255)
     aGCCTGCCGCGTTCTATCACCACAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGatcatc                                                                                                                                                <  5:42724/149‑1 (MQ=255)
       ccTGCCGCGTTCTATCACCACAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTGGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGc                                                                                                                                              >  1:76810/1‑149 (MQ=255)
         tGCCGCGTTCTATCACCACAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCt                                                                                                                                             >  2:103344/1‑148 (MQ=255)
           ccGCGTTCTATCACCACAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTaaa                                                                                                                                          >  4:292655/1‑149 (MQ=255)
               gTTCTATCACCACAATGTCATCCGCATGTGCGAAATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGc                                                                                                                                       >  6:333484/1‑148 (MQ=255)
                       accacAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGa                                                                                                                              <  8:301209/149‑1 (MQ=255)
                         cacaATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGa                                                                                                                              >  7:124265/1‑147 (MQ=255)
                                tcttCCGCATGTGCGACATATAACGAAGAATCGATGGTTAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGTCTCCCATTTTGGATCATCGCTTTATGCAAAACGTGAAAGCCGTg                                                                                                                      <  4:83612/145‑1 (MQ=255)
                                   tCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGt                                                                                                                  >  3:278968/1‑149 (MQ=255)
                                           tgCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCgg                                                                                                          >  2:57490/1‑149 (MQ=255)
                                             cGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGc                                                                                                         >  7:366127/1‑148 (MQ=255)
                                                   ataACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACaaa                                                                                                   >  1:136757/1‑148 (MQ=255)
                                                     aaCGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAg                                                                                                >  1:72507/1‑149 (MQ=255)
                                                     aaCGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAg                                                                                                >  1:19895/1‑149 (MQ=255)
                                                     aaCGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAg                                                                                                >  3:141210/1‑149 (MQ=255)
                                                      aCGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGc                                                                                               <  8:124265/149‑1 (MQ=255)
                                                      aCGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGc                                                                                               >  1:110002/1‑149 (MQ=255)
                                                       cGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGGGGCGTTTTACCGGCGACAAACAGCg                                                                                              <  2:76810/149‑1 (MQ=255)
                                                        gaagaaTCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGCATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGt                                                                                             >  5:259852/1‑149 (MQ=255)
                                                        gaagaaTCGATGGTAAACCTGGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGCATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGAGACAAACAGAGt                                                                                             >  5:254843/1‑149 (MQ=255)
                                                          agaaTCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGttt                                                                                           <  2:19895/149‑1 (MQ=255)
                                                          agaaTCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGttt                                                                                           <  6:45636/149‑1 (MQ=255)
                                                              tCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACt                                                                                       <  1:103344/149‑1 (MQ=255)
                                                              tCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACt                                                                                       >  4:110746/1‑149 (MQ=255)
                                                                gATGGTAAACCTTTCACCCCATTTCTTGACGTAAATCAACTCGGATTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTg                                                                                      <  6:256588/148‑1 (MQ=255)
                                                                gATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTg                                                                                      <  7:266002/148‑1 (MQ=255)
                                                                    gTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGt                                                                                  >  2:295989/1‑148 (MQ=255)
                                                                       aaacTTGCAACCCCTTTATTGACGTAAATCAACGCGGTTTCATTGTCATGAACGTGATGTCAGGACTCCCATTTTGCATCATCGCTAAATGCAAAAAGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTAACTGGCGTTAt                                                                               <  6:254843/145‑1 (MQ=255)
                                                                       aaCCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGCATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTAt                                                                               <  6:259852/148‑1 (MQ=255)
                                                                              cAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAg                                                                       >  2:177321/1‑149 (MQ=255)
                                                                               aaCCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGc                                                                      <  1:177321/149‑1 (MQ=255)
                                                                                aCCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCg                                                                     >  1:156118/1‑149 (MQ=255)
                                                                                aCCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCg                                                                     >  8:244465/1‑149 (MQ=255)
                                                                                    cTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGa                                                                 <  4:141210/149‑1 (MQ=255)
                                                                                     tttCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGa                                                                 >  7:366354/1‑148 (MQ=255)
                                                                                       tCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCACATTTTGTATAATCGCTTAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGTCTACATACAGCGTTTAACTGTCGTTATCAAAACAGCGTTGATa                                                               >  3:103100/1‑148 (MQ=255)
                                                                                              gTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACaa                                                       >  4:249768/1‑149 (MQ=255)
                                                                                                      aCTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATATTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTATTACCGGCGACAAACAGCGGTTCACTGGCGTTATCAACACAGCGTTGATCCATGACCACCGcccga                                               >  2:76811/1‑147 (MQ=255)
                                                                                                      aCTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATATTCGATCAACGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTATCACTGGCGTAATCAAAAct                                                                        >  6:60119/1‑123 (MQ=255)
                                                                                                        tCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAACCGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTAtt                                             >  3:43503/1‑149 (MQ=255)
                                                                                                        tCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTAtt                                             >  8:103186/1‑149 (MQ=255)
                                                                                                          ggTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTcc                                           <  8:366127/149‑1 (MQ=255)
                                                                                                          ggTTTCATTGTCATGAACGTGATGTCCGCACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCTGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTc                                            >  7:47414/1‑148 (MQ=255)
                                                                                                           gTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTAtgaca                                          >  8:254988/1‑145 (MQ=255)
                                                                                                            tttCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAACCGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCAt                                         <  4:43503/149‑1 (MQ=255)
                                                                                                               cATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGTGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGa                                       <  3:292655/148‑1 (MQ=255)
                                                                                                                    tCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGagcaagc                                 <  5:333484/149‑1 (MQ=255)
                                                                                                                      aTGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGagcaagca                                >  5:234092/1‑148 (MQ=255)
                                                                                                                      aTGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAgcaagcaa                               <  4:278968/149‑1 (MQ=255)
                                                                                                                         aaCGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCaaaaa                            <  6:234092/149‑1 (MQ=255)
                                                                                                                         aaCGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCaaaaa                            >  4:139812/1‑149 (MQ=255)
                                                                                                                           cGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACa                          <  7:244465/149‑1 (MQ=255)
                                                                                                                            gTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACACCCTCCCTATTCCATGAGCAAGCAAAACCaa                         >  4:84053/1‑149 (MQ=255)
                                                                                                                             tGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAat                        <  1:295989/149‑1 (MQ=255)
                                                                                                                              gATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAata                       >  3:26888/1‑149 (MQ=255)
                                                                                                                                  tCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAat                        >  6:351619/1‑144 (MQ=255)
                                                                                                                                  tCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAat                        <  5:351619/144‑1 (MQ=255)
                                                                                                                                   ccGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGc                  >  5:360193/1‑149 (MQ=255)
                                                                                                                                   ccGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTAATCCATGAGCAAGCAAAAACAATATATGc                  <  2:110002/149‑1 (MQ=255)
                                                                                                                                    cGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGCCAACCTCCCTATTCCATGAGCAAGCAAAAACAAAATATg                   >  3:279029/1‑147 (MQ=255)
                                                                                                                                           ccATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACCGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATaa           >  2:135646/1‑148 (MQ=255)
                                                                                                                                           ccATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATaa           >  1:72999/1‑148 (MQ=255)
                                                                                                                                              ttttGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATa       >  1:235136/1‑149 (MQ=255)
                                                                                                                                              ttttGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATa       <  1:1272/149‑1 (MQ=255)
                                                                                                                                                   gATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCg  >  3:96554/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CATGCAGCCTGCCGCGTTCTATCACCACAATGTCATCCGCATGTGCGACATATAACGAAGAATCGATGGTAAACCTTGCAACCCCTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCAAAACAGCGTTGATACATGACAACCTCCCTATTCCATGAGCAAGCAAAAACAATATATGCCGGATAAAATACGGCG  >  NC_000913/1779064‑1779359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: