Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,853,342 G→A 5.6% A213V (GCA→GTA)  ydjE ← putative MFS sugar transporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,853,3420GA 5.6% 230.3 / 2.3 72A213V (GCA→GTA) ydjEputative MFS sugar transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base G (33/34);  new base A (2/2);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

GTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCT  >  NC_000913/1853199‑1853485
                                                                                                                                               |                                                                                                                                               
gtAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCt                                                                                                                                            <  8:368857/149‑1 (MQ=255)
      tttCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGAtt                                                                                                                                      <  1:53072/149‑1 (MQ=255)
      tttCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGAtt                                                                                                                                      >  8:284187/1‑149 (MQ=255)
        tCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTg                                                                                                                                    >  1:84067/1‑149 (MQ=255)
           ccTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGccc                                                                                                                                 <  2:79628/149‑1 (MQ=255)
            cTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCt                                                                                                                                >  7:178475/1‑149 (MQ=255)
             tttAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCtt                                                                                                                               >  2:183538/1‑149 (MQ=255)
               tAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCtt                                                                                                                               <  3:66389/147‑1 (MQ=255)
                aaaCAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTACACCTGCGATTTGCCCTTTcc                                                                                                                            >  7:301493/1‑149 (MQ=255)
                aaaCAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTACACCTGCGATTTGCCCTTTc                                                                                                                             >  1:59861/1‑148 (MQ=255)
                       aGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCACTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTgccag                                                                                                                     >  4:137524/1‑149 (MQ=255)
                            gAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATc                                                                                                                >  1:244399/1‑149 (MQ=255)
                                      ttGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTc                                                                                                      >  6:87504/1‑149 (MQ=255)
                                        gATTACTTTAACCTTGCTTTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGTCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTc                                                                                                      <  6:200432/147‑1 (MQ=255)
                                        gATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGGAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCGGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTc                                                                                                      <  4:74652/147‑1 (MQ=255)
                                         aTTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGa                                                                                                                                        <  6:200655/112‑1 (MQ=255)
                                             cTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATaaa                                                                                                <  4:40125/148‑1 (MQ=255)
                                             cTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAg                                                                                               >  1:101019/1‑149 (MQ=255)
                                              tttAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGAtt                                                                                                                                      >  1:195843/1‑109 (MQ=255)
                                              tttAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGAtt                                                                                                                                      <  2:195843/109‑1 (MQ=255)
                                              tttAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAg                                                                                               >  1:175303/1‑148 (MQ=255)
                                                  aCCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTAttt                                                                                          <  5:87504/149‑1 (MQ=255)
                                                  aCCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTAttt                                                                                          <  2:65945/149‑1 (MQ=255)
                                                  aCCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTACACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGTTAAAGTAttt                                                                                          <  8:301493/149‑1 (MQ=255)
                                                  aCCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTACACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTAttt                                                                                          <  2:59861/149‑1 (MQ=255)
                                                    cTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTAc                                                                                        <  2:84067/149‑1 (MQ=255)
                                                    cTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTAc                                                                                        >  3:40269/1‑149 (MQ=255)
                                                         tGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCt                                                                                      >  5:174717/1‑146 (MQ=255)
                                                           ttGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTGCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTgag                                                                                   <  7:284187/147‑1 (MQ=255)
                                                              ctctGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGaaa                                                                              <  8:273271/149‑1 (MQ=255)
                                                               tctGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAAc                                                                             >  7:140273/1‑149 (MQ=255)
                                                               tctGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACATCACGAAGTAGGCATTCTCCACCTGCGATATGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAAc                                                                             >  6:8191/1‑149 (MQ=255)
                                                                 tGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTTCTGCTCTACTTAACGAAGTTGGCATTCTGAACCTGCGATTTGCCCTTTCCCTGCCCGCCATCTTGTCGCATCGATAAACTATTTACCTGAGAGAAAc                                                                             >  3:265498/1‑147 (MQ=255)
                                                                 tGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAAc                                                                             >  2:304558/1‑147 (MQ=255)
                                                                  gATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCCTGGCGACTCGAT‑‑AGTATTTACCTGAGAGAAACCAgg                                                                         >  8:225784/1‑148 (MQ=255)
                                                                      cGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCt                                                                       >  2:300879/1‑148 (MQ=255)
                                                                       gAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTaa                                                                     >  3:24384/1‑149 (MQ=255)
                                                                       gAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTaa                                                                     <  2:244399/149‑1 (MQ=255)
                                                                         aGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACa                                                                   <  1:183538/149‑1 (MQ=255)
                                                                          gTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACATGCGATTTGCCCTTTACCTGCCAGCCATCGTGGCGACTCGATAAAGAATTTACCTGAGAGAAACCAGGCTAACAg                                                                  >  1:311491/1‑149 (MQ=255)
                                                                               cGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGc                                                             <  2:175303/149‑1 (MQ=255)
                                                                                    gTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCACTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATa                                                        <  3:137524/149‑1 (MQ=255)
                                                                                          gAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCt                                                                                                                                <  3:19561/71‑1 (MQ=255)
                                                                                          gAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCt                                                                                                                                >  4:19561/1‑71 (MQ=255)
                                                                                          gAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCa                                                                           >  8:122252/1‑124 (MQ=255)
                                                                                          gAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCa                                                                           <  7:122252/124‑1 (MQ=255)
                                                                                          gAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATaccacc                                                   >  1:52645/1‑148 (MQ=255)
                                                                                             tACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATa                                                        >  3:65573/1‑140 (MQ=255)
                                                                                             tACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATa                                                        <  4:65573/140‑1 (MQ=255)
                                                                                                  ttctCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGaaa                                          >  4:232423/1‑149 (MQ=255)
                                                                                                    ctctctTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGaaa                                          <  1:300879/147‑1 (MQ=255)
                                                                                                    ctctctTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAAc                                         <  1:304558/148‑1 (MQ=255)
                                                                                                     tctctTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTa                                                                <  8:204259/124‑1 (MQ=255)
                                                                                                     tctctTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTa                                                                >  7:204259/1‑124 (MQ=255)
                                                                                                     tctctTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACat                                       >  2:174113/1‑149 (MQ=255)
                                                                                                      ctctTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACat                                       >  7:22238/1‑148 (MQ=255)
                                                                                                         tttCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACatta                                     <  2:52645/147‑1 (MQ=255)
                                                                                                          ttCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAACCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTc                                  >  5:25195/1‑149 (MQ=255)
                                                                                                            cAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAg                                                                          <  6:200465/107‑1 (MQ=255)
                                                                                                                    tgctCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTaa                         >  6:339905/1‑148 (MQ=255)
                                                                                                                         tACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAg                   >  5:160393/1‑149 (MQ=255)
                                                                                                                           cTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGc                  <  4:24384/148‑1 (MQ=255)
                                                                                                                           attgaCGTAGTTGTCATTGTGTAACTGCGATTTTGCCTTTCACTGGCATGCATCTTGGGTTATCGATAAAGTATTTACCTGTGTGAAAACATGCTTACAGTATTCCAATTACACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGc                  <  4:265498/144‑1 (MQ=255)
                                                                                                                             tCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGc                                >  1:270439/1‑132 (MQ=255)
                                                                                                                             tCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGc                                <  2:270439/132‑1 (MQ=255)
                                                                                                                              cACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGa                <  3:335162/147‑1 (MQ=255)
                                                                                                                              cACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGa                <  3:232423/147‑1 (MQ=255)
                                                                                                                              cACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGa                <  5:339905/147‑1 (MQ=255)
                                                                                                                                        gCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGc    <  1:174113/149‑1 (MQ=255)
                                                                                                                                           ttCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACatta                                     <  3:137251/113‑1 (MQ=255)
                                                                                                                                           ttCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACatta                                     >  4:137251/1‑113 (MQ=255)
                                                                                                                                           ttCTGCACCTGCGATTTGCCATTTCCCTGCCAGCCATCGTGGCGAATCGATAAAGTATTTACCTTAGAGAAACAAGGCTAACAGTATGCCAATACCACCAATCAGAAACATTATTCGCCAACTAAAAACAGCGATAACCACCACGCCt  >  1:310503/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCT  >  NC_000913/1853199‑1853485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: