Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,980,242	T→G	6.9%	R457R (CGA→CGC)	otsA ←	trehalose‑6‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,980,242	0	T	G	6.9%	258.6 / 11.9	87	R457R (CGA→CGC)	otsA	trehalose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (39/42);  new base G (3/3);  total (42/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-01

TTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAGCGCAGCTG  >  NC_000913/1980094‑1980384                                                                                                                                                     |                                                                                                                                               tttCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCtt                                                                                                                                                >  3:210924/1‑149 (MQ=255) tttCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCtt                                                                                                                                                <  3:306071/149‑1 (MQ=255)    cTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTcgc                                                                                                                                             >  3:167277/1‑149 (MQ=255)       ttCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTcgcg                                                                                                                                            <  2:105112/147‑1 (MQ=255)        tCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGaa                                                                                                                                         >  3:131232/1‑149 (MQ=255)         cgcgAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGaa                                                                                                                                         <  6:116430/148‑1 (MQ=255)          gcgAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAAc                                                                                                                                        <  8:25710/148‑1 (MQ=255)            gAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTGTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTAt                                                                                                                                     >  2:112795/1‑149 (MQ=255)             aGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATc                                                                                                                                    <  4:135648/149‑1 (MQ=255)                 tCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCt                                                                                                                                >  4:149862/1‑149 (MQ=255)                 tCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCt                                                                                                                                >  1:269562/1‑149 (MQ=255)                      cTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTa                                                                                                                             <  5:321706/147‑1 (MQ=255)                      cTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAgg                                                                                                                           <  4:175288/149‑1 (MQ=255)                         ggCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTa                                                                                                                             >  2:157622/1‑144 (MQ=255)                         ggCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTa                                                                                                                             <  1:157622/144‑1 (MQ=255)                         ggCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGt                                                                                                                          <  7:203147/147‑1 (MQ=255)                          gCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGc                                                                                                                       >  4:84968/1‑149 (MQ=255)                            gAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGAAGCGCGGaaagcc                                                                                                                                     <  8:89929/133‑5 (MQ=255)                            gAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGAAGCGCGGaaagcc                                                                                                                                     >  7:89929/1‑129 (MQ=255)                               aaGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAAt                                                                                                                   >  8:150244/1‑148 (MQ=255)                                agagTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGa                                                                                                                 <  4:167277/149‑1 (MQ=255)                                 gagTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGaa                                                                                                                <  3:262577/149‑1 (MQ=255)                                  agTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAg                                                                                                               <  6:78839/149‑1 (MQ=255)                                   gTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGc                                                                                                              <  7:150244/149‑1 (MQ=255)                                    ttATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCa                                                                                                             <  3:149862/149‑1 (MQ=255)                                    ttATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCa                                                                                                             <  4:131232/149‑1 (MQ=255)                                      aTTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACATACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCAc                                                                                                            <  2:310607/148‑1 (MQ=255)                                      aTTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACATACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCAc                                                                                                            >  1:310607/1‑148 (MQ=255)                                          ccTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTg                                                                                                       >  7:211164/1‑149 (MQ=255)                                                gTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTTGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTg                                                                                                 <  5:220638/149‑1 (MQ=255)                                                 tGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGAAGCGCGGaaagcc                                                                                                                                     <  6:141019/112‑5 (MQ=255)                                                 tGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGAAGCGCGGaaagcc                                                                                                                                     >  5:141019/1‑108 (MQ=255)                                                   aGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGt                                                                                               >  6:103997/1‑148 (MQ=255)                                                        gATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAata                                                                                          <  1:286422/148‑1 (MQ=255)                                                         aTGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATc                                                                                        >  4:82759/1‑149 (MQ=255)                                                            tgCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGtt                                                                                     <  3:84968/149‑1 (MQ=255)                                                            tgCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGt                                                                                      <  3:59159/148‑1 (MQ=255)                                                              cTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGtttt                                                                                   >  7:157715/1‑149 (MQ=255)                                                              cTGTTAGTTCCACTTACGGGAGAGTAACCGCTCCTACGCAAGCTTTGGAGAGGTAGCAACTTTATCGCGCTGCTGTCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGtttt                                                                                   <  7:314574/149‑1 (MQ=255)                                                               tGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGttttt                                                                                  >  7:130024/1‑149 (MQ=255)                                                                gTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGttttt                                                                                  >  1:340279/1‑148 (MQ=255)                                                                     ttCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGa                                                                             >  7:198053/1‑148 (MQ=255)                                                                      tCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGa                                                                             >  8:236486/1‑147 (MQ=255)                                                                      tCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGa                                                                             <  7:236486/147‑1 (MQ=255)                                                                         aCTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCa                                                                                >  1:124701/1‑141 (MQ=255)                                                                         aCTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCa                                                                                <  2:124701/141‑1 (MQ=255)                                                                         aCTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACg                                                                        >  4:318799/1‑149 (MQ=255)                                                                           ttACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTc                                                                      >  7:236078/1‑149 (MQ=255)                                                                             aCGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTc                                                                      >  8:215796/1‑147 (MQ=255)                                                                                ggAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCa                                                                 >  1:118407/1‑149 (MQ=255)                                                                                 gagaTTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTTCTTTAGGTAGCTAATGAAGCACTCCTGCCAGTGGTTAATATCCTTTTTCACGATAACGTCCAGCAt                                                                >  7:260994/1‑149 (MQ=255)                                                                                 gagaTTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCAt                                                                >  6:173167/1‑149 (MQ=255)                                                                                   gaTTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCAttt                                                              <  4:6649/149‑1 (MQ=255)                                                                                     ttAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATTACGTCCAGCATTTCt                                                            <  3:277619/149‑1 (MQ=255)                                                                                     ttAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCt                                                            >  8:123048/1‑149 (MQ=255)                                                                                     ttAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCt                                                            <  5:103997/149‑1 (MQ=255)                                                                                      tAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTg                                                           >  7:340193/1‑149 (MQ=255)                                                                                        aCCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCa                                                         <  3:135373/149‑1 (MQ=255)                                                                                         ccGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTGAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCAt                                                        >  5:74506/1‑149 (MQ=255)                                                                                         ccGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCACTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCAt                                                        >  8:361566/1‑149 (MQ=255)                                                                                               ctccGCAAGGTTTGGAATGGTCGCAACTTTATCGCGCGGGTGGCTTCCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATTACGCACTCCTGCCTGTGGTTAATTTGGTTTTTCACTATAACGACCCGCATTTGTGCATGAggg                                                   <  8:236078/145‑1 (MQ=255)                                                                                               cTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCAttt                                                              <  6:170413/137‑1 (MQ=255)                                                                                               cTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACg                                                    <  8:340193/147‑1 (MQ=255)                                                                                               cTACGCAAGCTTTGGAAAGGTAGCAACTTTATAGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCAttt                                                              >  5:170413/1‑137 (MQ=255)                                                                                                  cGCAAGCTTTGGAAAGGTAGCAACTTTATCGCACTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGa                                                 <  7:361566/147‑1 (MQ=255)                                                                                                    cAAGCTTTGGAAAGGTAGCAACATTATCGCGCTGCAGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAATGGTTAATATCGATTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATa                                             >  1:103341/1‑149 (MQ=255)                                                                                                     aaGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATAc                                            <  2:118407/149‑1 (MQ=255)                                                                                                            ggAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGc                                     >  1:68368/1‑149 (MQ=255)                                                                                                               aaGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAg                                  <  7:123048/149‑1 (MQ=255)                                                                                                                   tAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGa                               >  8:146128/1‑148 (MQ=255)                                                                                                                     gCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACa                             >  8:321540/1‑148 (MQ=255)                                                                                                                     gCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACa                             <  5:173167/148‑1 (MQ=255)                                                                                                                     gCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACAt                            <  8:157715/149‑1 (MQ=255)                                                                                                                      cAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACa                             >  6:207635/1‑147 (MQ=255)                                                                                                                      cAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATa                           <  2:269562/149‑1 (MQ=255)                                                                                                                       aaCTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTAGCTAAGGAAGCACTACTGCCAGTGGTTAATATCATTTGTCACGATAACGGCCAGCAGTTCTGCATGAACGGAAAt                                              >  8:254832/1‑129 (MQ=255)                                                                                                                       aaCTTTATCGCGCTGATGGCTTTCCGCGCTTAGAGGAACTATCTGCTTTATGGAGCTAATGACGCACTACTGCCCGTGGTTACTCTCGTTGTTCACGATAAAGTCCAGCATTTCTGCATGACGGGAAAt                                              <  7:254832/129‑1 (MQ=255)                                                                                                                          tttATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTc                        >  7:132172/1‑148 (MQ=255)                                                                                                                            tATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCaa                      <  3:82759/148‑1 (MQ=255)                                                                                                                                      ctgGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGc                                                                                                                       <  2:333697/41‑1 (MQ=255)                                                                                                                                      ctgGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGc                                                                                                                       >  1:333697/1‑41 (MQ=255)                                                                                                                                      ctgGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCa           <  8:198053/149‑1 (MQ=255)                                                                                                                                      ctgGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCa           <  8:211164/149‑1 (MQ=255)                                                                                                                                       tgGCTTACCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACACCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCAATTCTGCATGACGTGAAAAACGTTCCGCAAGCGACATAGTCAATGCACCATCCAg          >  6:52786/1‑149 (MQ=255)                                                                                                                                        atagttCCGCGAAGCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGt                         <  8:199060/129‑1 (MQ=255)                                                                                                                                        atagttCCGCGAAGCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGt                         >  7:199060/5‑133 (MQ=255)                                                                                                                                         gCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAgc         <  8:130024/148‑1 (MQ=255)                                                                                                                                             tacgcgCTTCGCGGAACTTTCTGCTTTAGTTCGATAATGACGCACTCCTGCCAGTGGTTAATATCGTGTTTCACGATAACGTCGAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAGCGCa      <  8:260994/145‑1 (MQ=255)                                                                                                                                               cgcgCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAGCGCAGCTg  >  5:95738/1‑149 (MQ=255)                                                                                                                                                    ttCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGc                   <  8:288539/127‑1 (MQ=255)                                                                                                                                                    ttCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGc                   >  7:288539/1‑127 (MQ=255)                                                                                                                                                     |                                                                                                                                               TTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAGCGCAGCTG  >  NC_000913/1980094‑1980384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑