| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,868,859 | 0 | C | . | 20.6% | 92.8 / 21.7 | 34 | intergenic (+17/‑96) | yeaG/yeaH | protein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein |
| Reads supporting (aligned to +/- strand): ref base C (13/14); new base . (3/4); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGG > NC_000913/1868713‑1869003 | cGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTaca > 5:39336/1‑148 (MQ=255) cAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGt > 8:59074/1‑149 (MQ=255) gTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCg < 6:39336/149‑1 (MQ=255) ccGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGcc > 5:126688/1‑148 (MQ=255) ccGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGTGTACCGGGcca < 5:124073/149‑2 (MQ=255) ccGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGTGTACCGGGcca < 5:122491/149‑2 (MQ=255) acgaGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGTGTACCGGGccaggg < 7:59074/149‑5 (MQ=255) acacGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGccagg < 3:45663/135‑4 (MQ=255) acacGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGccagg > 4:45663/1‑132 (MQ=255) gacgacTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCgg > 2:80729/1‑125 (MQ=255) gacgacTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCgg < 1:80729/125‑1 (MQ=255) gTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCaa < 6:126688/149‑1 (MQ=255) tATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCaa > 3:138842/1‑148 (MQ=255) tgatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAaca > 7:31016/1‑148 (MQ=255) ggAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGc > 2:122253/1‑149 (MQ=255) ccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGATgg < 8:60706/149‑1 (MQ=255) tGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtagta < 4:154431/149‑1 (MQ=255) gTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTac < 2:52648/61‑1 (MQ=255) gTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTac > 1:52648/1‑61 (MQ=255) cGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATg < 1:122253/149‑1 (MQ=255) aaTGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGAc > 1:73315/1‑149 (MQ=255) tttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGtt < 4:138842/148‑1 (MQ=255) tttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGGGGGCCTGAGAAGc > 4:100835/1‑89 (MQ=255) tGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTa < 2:73315/148‑1 (MQ=255) gcgcGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTAt < 8:31016/148‑1 (MQ=255) cTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTg > 4:54989/1‑125 (MQ=255) cTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTg < 3:54989/125‑1 (MQ=255) ttAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCatat > 5:100359/1‑118 (MQ=255) ttAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCatat < 6:100359/118‑1 (MQ=255) acaacCCTGGCCCCGTACACATGT‑TACCGGGCCAACAACGACAGCGAACCGAGCGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACCGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCaa > 8:95051/1‑148 (MQ=255) taggCCCGGTA‑ACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg > 2:44617/3‑69 (MQ=25) taggCCCGGTA‑ACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg < 1:44617/67‑1 (MQ=25) cTGGCCCGGTACACATGT‑TACCGGGCCTATAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGaa < 2:134551/136‑1 (MQ=255) cTGGCCCGGTACACATGT‑TACCGGGCCTATAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGaa > 1:134551/1‑136 (MQ=255) cccGGTA‑ACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCa > 6:160743/1‑149 (MQ=255) gTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATgg > 2:77932/1‑148 (MQ=255) gTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATgg < 1:77932/148‑1 (MQ=255) | CGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGG > NC_000913/1868713‑1869003 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |