Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,795,753	C→A	G731V (GGC→GTC)	pheT ←	phenylalanine tRNA synthetase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,795,753	0	C	A	100.0%	70.1 / NA	21	G731V (GGC→GTC)	pheT	phenylalanine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/10);  total (11/10)

TCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGTTTTCTGCGACCACCACCGCGATGTCACGACGGTTCGCCGGGAAGCGAGAAATCTCGCGCGCCTGAGGCACCACGCGGTCTGCGAGCTTGTTCCA  >  NC_000913/1795625‑1795889                                                                                                                                 |                                                                                                                                         tctcCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGACAACTTTCtt                                                                                                                                <  3:152759/139‑1 (MQ=255) tctcCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGACAACTTTCtt                                                                                                                                >  4:152759/1‑139 (MQ=255)     ctctTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGACAACTTTCTTACATTCGGATaaaa                                                                                                                  <  1:68631/149‑1 (MQ=255)                              tCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGACAACTTTCTTACATTCGGATaaaa                                                                                                                  >  4:69814/1‑124 (MQ=255)                              tCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGACAACTTTCTTACATTCGGATaaaa                                                                                                                  <  3:69814/124‑1 (MQ=255)                               cTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGACAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGTTTTCTGc                                                                                         >  8:123949/1‑148 (MQ=255)                                       aTCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTGACGACAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGTTTTCTGCGaccaccac                                                                                >  5:153756/1‑149 (MQ=255)                                                 tGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGACAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGTTTTCTGCGACCACCACCGCGATGTc                                                                       <  7:123949/148‑1 (MQ=255)                                                                    cTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGACAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACg                                                                                                 <  8:134747/103‑1 (MQ=255)                                                                    cTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGACAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACg                                                                                                 <  8:132625/103‑1 (MQ=255)                                                                    cTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGACAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACg                                                                                                 >  7:134747/1‑103 (MQ=255)                                                                    cTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGACAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACg                                                                                                 >  7:132625/1‑103 (MQ=255)                                                                    cTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGACAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGTTTTCTGCGACCACCACCGCGATGTCACGACGGTTCGCCGGGAAGc                                                   <  6:153756/149‑1 (MQ=255)                                                                          aaCACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGACAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGTTTTCTGCGACCACCACCGCGATGTCACGACGGTTCGCCGGGAAGCGAGaaa                                             >  1:20620/1‑149 (MQ=255)                                                                                     cgcgGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGACAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGTTTTCTGCGACCACCACCGCGATGTCACGACGGTTCGCCGGGAAGCGAGAAATCTCGCGCGcc                                  >  2:184482/1‑149 (MQ=255)                                                                                             acGTCAAATAAGTTTACGCCAACTACCTGATTTACGACAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGtttt                                                                                             >  2:68415/1‑82 (MQ=255)                                                                                             acGTCAAATAAGTTTACGCCAACTACCTGATTTACGACAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGtttt                                                                                             <  1:68415/82‑1 (MQ=255)                                                                                                       aGTTTACGCCAACTACCTGATTTACGACAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGTTTTCTGCGACCACCACCGCGATGTCACGACGGTTCGCCGGGAAGCGAGAAATCTCGCGCGCCTGAGGCACCACGCGGTc                 <  1:184482/148‑1 (MQ=255)                                                                                                                      ccTGATTTACGACAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGTTTTCTGCGACCACCACCGCGATGTCACGACGGTTCGCCGGGAAGCGAGAAATCTCGCGCGCCTGAGGCACCACGCGGTCTGCGAGCTTGTTCCa  >  2:82599/1‑148 (MQ=255)                                                                                                                           tttACGACAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGTTTTCTGCGACCACCACCGCGATGTCacgacg                                                                 <  2:130958/80‑1 (MQ=255)                                                                                                                           tttACGACAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGTTTTCTGCGACCACCACCGCGATGTCacgacg                                                                 >  1:130958/1‑80 (MQ=255)                                                                                                                                 |                                                                                                                                         TCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGTTTTCTGCGACCACCACCGCGATGTCACGACGGTTCGCCGGGAAGCGAGAAATCTCGCGCGCCTGAGGCACCACGCGGTCTGCGAGCTTGTTCCA  >  NC_000913/1795625‑1795889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑