Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,319	C→T	intergenic (‑236/‑51)	ydiY ← / → pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,319	0	C	T	100.0%	122.0 / NA	35	intergenic (‑236/‑51)	ydiY/pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/20);  total (15/20)

ATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCG  >  NC_000913/1806179‑1806463                                                                                                                                             |                                                                                                                                                 ataAAAAGGCGCGAATAACTTATCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTcc                                                                                                                                           <  1:39476/148‑1 (MQ=255)  taAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGc                                                                                                                                         <  2:89127/149‑1 (MQ=255)           gcgAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGt                                                                                                                                >  8:62605/1‑149 (MQ=255)             gAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGc                                                                                                                              >  5:1326/1‑149 (MQ=255)                    ttAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACt                                                                                                                        >  7:113585/1‑148 (MQ=255)                          aTGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGaaa                                                                                                                    <  1:5553/146‑1 (MQ=255)                          aTGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGaaa                                                                                                                    >  2:5553/1‑146 (MQ=255)                                tCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATCTTGTTAATTTCTTTACTTTCCGCGGATTCGGTGCCAGACTGTAAGCAGCCta                                                                                                            <  4:59798/148‑1 (MQ=255)                                             tttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatga                                                                                               <  1:190562/148‑1 (MQ=255)                                                 aaTAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGt                                                                                           <  8:113585/148‑1 (MQ=255)                                                  aTAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTa                                                                                          <  1:579/148‑1 (MQ=255)                                                               gAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                            <  7:62605/149‑1 (MQ=255)                                                                     aGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCa                                                                                                                            >  8:18036/1‑95 (MQ=255)                                                                     aGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCa                                                                                                                            <  7:18036/95‑1 (MQ=255)                                                                               ttatATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctct                                                            >  3:146/1‑149 (MQ=255)                                                                                    tatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGa                                                       <  4:146/149‑1 (MQ=255)                                                                                      tatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATa                                                     <  6:1326/149‑1 (MQ=255)                                                                                       atTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGacac                                                                       >  8:105199/1‑130 (MQ=255)                                                                                       atTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGacac                                                                       <  7:105199/130‑1 (MQ=255)                                                                                               gCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCtctctc                                                         >  3:99160/1‑136 (MQ=255)                                                                                               gCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCtctctc                                                         <  4:99160/136‑1 (MQ=255)                                                                                                  aaaTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGc                                                                                                               >  8:43098/1‑79 (MQ=255)                                                                                                  aaaTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGc                                                                                                               <  7:43098/79‑1 (MQ=255)                                                                                                       ttAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATagga                                                                                                       >  8:7833/1‑82 (MQ=255)                                                                                                       ttAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATagga                                                                                                       <  7:7833/82‑1 (MQ=255)                                                                                                          aataaaGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaacaa                                            >  4:33777/1‑138 (MQ=255)                                                                                                          aataaaGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaacaa                                            <  3:33777/138‑1 (MQ=255)                                                                                                                gCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCtat                                                                                 >  1:35776/1‑95 (MQ=255)                                                                                                                gCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCtat                                                                                 <  2:35776/95‑1 (MQ=255)                                                                                                                 cTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGcc                                                                <  2:141625/111‑1 (MQ=255)                                                                                                                 cTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGcc                                                                >  1:141625/1‑111 (MQ=255)                                                                                                                    cAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCtctctc                                                         <  6:6275/115‑1 (MQ=255)                                                                                                                    cAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCtctctc                                                         >  5:6275/1‑115 (MQ=255)                                                                                                                             atatTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGacac                                                                       <  4:130290/92‑1 (MQ=255)                                                                                                                             atatTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGacac                                                                       >  3:130290/1‑92 (MQ=255)                                                                                                                                         tctttACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCg  >  2:139823/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                                 ATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCG  >  NC_000913/1806179‑1806463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑