Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC NC_000913 1,757,082 IS5 (–) +4 bp 27.3% coding (1385‑1388/1413 nt) pykF → pyruvate kinase I

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1397238NA (NA)11 (0.120) 11/266 NT 30.8% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1757085 24 (0.270)coding (1388/1413 nt) pykF pyruvate kinase I
* ? NC_000913 1757082 =24 (0.270)7 (0.080) 6/266 NT 22.0% coding (1385/1413 nt) pykF pyruvate kinase I
?NC_000913 = 2102943 NA (NA)noncoding (1/1195 nt) IS5 repeat region

CGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1397106‑1397238
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGTAGTGCCGCTCGGTACCAGTGCACCAGAAACCATAACTACAACGTCACCTTTGTGTGCCAGACCGCTCTGCAGAGCCAGTTCTTTACCCAGACGGTAGAAATCATCAGTAGAAGTGATCTCTTTAACAAGC  <  NC_000913/1757085‑1756951
                                                                                                                                                                                                                                                                            
CGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAGTAGTGCCGCTCG                                                                                                                         <  3:90968/149‑1
       AATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAGTAGTGCCGCTCGGTACCA                                                                                                                   <  3:118536/148‑1
               AGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAGTAGTGCCGCTCGGTACCAGTGCAC                                                                                                             >  3:148705/1‑146
                       TCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAGTAGTGCCGCTCGGTACCAGTGCACCAGAAACCAT                                                                                                   >  5:371381/1‑148
                          AAATAGGCTGATTCAAGGCATTTACGGGAGAGAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAAATTCCCCGCTTATTCGCACCTTCCTTAGTAGTGCCGCTCGGTACCAGAGCACCAGAAACCATAAC                                                                                                >  5:387451/1‑148
                                        AAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAGTAGTGCCGCTCGGTACCAGTGCACCAGAAACCATAACTACAACGTCACCTTT                                                                                 <  1:183543/149‑1
                                          GGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAGTAGTGCCGCTCGGTACCAGTGCACCAGAAACCATAACTACAACGTCACCTTTGT                                                                               <  4:148705/149‑1
                                              TTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAGTAGTGCCGCTCGGTACCAGTGCACCAGAAACCATAACTACAACGTCACCTTTGTGTGC                                                                           >  4:266223/1‑149
                                                             ATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAGTAGTGCCGCTCGGTACCAGTGCACCAGAAACCATAACTACAACGTCACCTTTGTGTGCCAGACCGCTCTGCAG                                                            <  3:266223/149‑1
                                                                GGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAGTAGTGCCGCTCGGTACCAGTGCACCAGAAACCATAACTACAACGTCACCTTTGTGTGCCAGACCGCTCTGCAGAG                                                          <  6:296754/148‑1
                                                                                                                       TATTCGCACCTTCCTTAGTAGTGCCGCTCGGTACCAGTGCACCAGAAACCATAACTACAACGTCACCTTTGTGTGCCAGACCGCTCTGCAGAGCCAGTTCTTTACCCAGACGGTAGAAATCATCAGTAGAAGTGATCTCTTTAACAAGC  >  8:121409/1‑149
                                                                                                                                                                                                                                                                            
CGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1397106‑1397238
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGTAGTGCCGCTCGGTACCAGTGCACCAGAAACCATAACTACAACGTCACCTTTGTGTGCCAGACCGCTCTGCAGAGCCAGTTCTTTACCCAGACGGTAGAAATCATCAGTAGAAGTGATCTCTTTAACAAGC  <  NC_000913/1757085‑1756951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.