Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,525,331 T→C 5.6% H217R (CAC→CGC)  ansP ← L‑asparagine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,525,3310TC 5.6% 221.3 / 3.1 72H217R (CAC→CGC) ansPL‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base T (26/41);  new base C (2/2);  total (28/44)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.21e-01

CAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCA  >  NC_000913/1525183‑1525475
                                                                                                                                                    |                                                                                                                                                
cAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACTCTACGCCCTTAATCAACACCAGCGCAGGCAGCAAACCGt                                                                                                                                                  <  4:377284/149‑1 (MQ=255)
 aGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGGCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTg                                                                                                                                                 <  5:26024/149‑1 (MQ=255)
  ggCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTgg                                                                                                                                                <  1:404957/149‑1 (MQ=255)
   gacAATACGCCAACTCACACTGTTAATGGCTTTAGGCACCATGGGCTGCGGATCTATACATTCTCCTGCAGCTGTACCCACCATTTCTATTGAGGCAAAAGCAAACAATACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTggg                                                                                                                                               <  1:30797/147‑1 (MQ=255)
   gCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTggg                                                                                                                                               <  7:305668/149‑1 (MQ=255)
     cAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCCTTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACCCCAGCGCAGGCAGCAAACCGTgggg                                                                                                                                              >  4:261379/1‑148 (MQ=255)
       cgatctcAAATCACACTCTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCTCAGGCAGCAAACCGTGGGGgaa                                                                                                                                           <  3:220233/143‑1 (MQ=255)
       aTACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGgaa                                                                                                                                           <  4:368816/149‑1 (MQ=255)
       aTACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGgaa                                                                                                                                           <  3:95290/149‑1 (MQ=255)
             cAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACACACCATTTCAATGGAGGAAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAgcc                                                                                                                                     >  3:230065/1‑149 (MQ=255)
              aaaTCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTGTCCGGCAGATGTACCCACCATTTCAATGGAGTCAAAAGCAAACACTACGCCGTGAATCAACACCAGCTCAGGCAGCACTCCGTGGGGgcagagagcc                                                                                                                                    >  4:220233/1‑144 (MQ=255)
               aaTCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAgccgc                                                                                                                                   >  5:399466/1‑149 (MQ=255)
                   acacTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCAt                                                                                                                                >  4:153692/1‑148 (MQ=255)
                         ttAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCgg                                                                                                                          <  5:743/148‑1 (MQ=255)
                         ttAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCg                                                                                                                           <  8:167385/147‑1 (MQ=255)
                           aaTGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGa                                                                                                                       >  1:387718/1‑149 (MQ=255)
                             tGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGAtt                                                                                                                     <  1:349289/149‑1 (MQ=255)
                                cTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCGTTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTaaa                                                                                                                  <  7:184178/149‑1 (MQ=255)
                                cTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTaaa                                                                                                                  <  6:399466/149‑1 (MQ=255)
                                   tAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATg                                                                                                                >  1:357481/1‑148 (MQ=255)
                                      gCACGATGGTCTGCGTATCTTTACCTTCTCCGGCAGCTGTCCCCACCATTTCAATGGAGGCATAAGCAATCACTACGCACTGAATCATCACCAGCTCAGGGAGCAAACCGTGGGGTAATCAGCCGCCATAATCGGTTATTAAATGaaa                                                                                                             <  6:321076/148‑1 (MQ=255)
                                      gCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGaaa                                                                                                             <  2:387718/148‑1 (MQ=255)
                                      gCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAg                                                                                                            <  3:79914/149‑1 (MQ=255)
                                       cACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGc                                                                                                           <  3:247950/149‑1 (MQ=255)
                                          cATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAgccgcc                                                                                                                                  >  7:204407/1‑123 (MQ=255)
                                          cATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAgccgcc                                                                                                                                  <  8:204407/123‑1 (MQ=255)
                                            tGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAATAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTCTCGGTGATTAAATGAAAGCCAgtg                                                                                                      <  2:25002/149‑1 (MQ=255)
                                            tGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAgtg                                                                                                      <  4:150681/149‑1 (MQ=255)
                                            tGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAgtg                                                                                                      <  3:330979/149‑1 (MQ=255)
                                                  gCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGgtgt                                                                                                  <  2:346015/147‑1 (MQ=255)
                                                  gCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGgtgt                                                                                                  >  1:346015/1‑147 (MQ=255)
                                                   cGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGtt                                                                                                 <  4:159727/147‑1 (MQ=255)
                                                      agcTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCACAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCAt                                                                                            <  4:289607/147‑1 (MQ=255)
                                                        cTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTggggg                                                                                                                                             <  1:65417/98‑1 (MQ=255)
                                                        cTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTggggg                                                                                                                                             >  2:65417/1‑98 (MQ=255)
                                                           tACATTCTCCGGCAGCTGTACCCACCATTTCCATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACTCCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATc                                                                                           <  7:92369/145‑1 (MQ=255)
                                                                  tcCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAAAACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGAc                                                                                >  2:335449/1‑149 (MQ=255)
                                                                    cGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGa                                                                                 <  3:198678/146‑1 (MQ=255)
                                                                            gTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATTGGTGATTAAATGAAAGCCAGTGGTGTTGCCCTCCAGCGGATGACCACTACCGAg                                                                      >  6:115922/1‑149 (MQ=255)
                                                                             tACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAg                                                                      <  1:89090/148‑1 (MQ=255)
                                                                                 caccaTTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAaca                                                                 <  2:357481/149‑1 (MQ=255)
                                                                                     aTTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGgtgt                                                                                                  >  6:232832/1‑112 (MQ=255)
                                                                                     aTTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGgtgt                                                                                                  <  5:232832/112‑1 (MQ=255)
                                                                                      tttCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTa                                                            >  3:373331/1‑149 (MQ=255)
                                                                                           aTGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCAc                                                       <  2:99990/149‑1 (MQ=255)
                                                                                           aTGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCAc                                                       >  6:186812/1‑149 (MQ=255)
                                                                                            tGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTAACGAGGAACACTGTACCCACg                                                      >  1:48629/1‑149 (MQ=255)
                                                                                                    aaaGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCaaaaa                                              <  5:186812/149‑1 (MQ=255)
                                                                                                    aaaGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCaaaaa                                              <  1:401101/149‑1 (MQ=255)
                                                                                                     aaGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAg                                             <  3:153692/149‑1 (MQ=255)
                                                                                                          aaCACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCa                                                        >  7:247140/1‑133 (MQ=255)
                                                                                                          aaCACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCa                                                        <  8:247140/133‑1 (MQ=255)
                                                                                                           acacTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTcaca                                       <  1:356337/149‑1 (MQ=255)
                                                                                                           acacTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTc                                          >  5:367668/1‑146 (MQ=255)
                                                                                                           acacTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTc                                          <  6:367668/146‑1 (MQ=255)
                                                                                                              cTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACAACCAAAAAGGTCACAATg                                    >  4:237604/1‑149 (MQ=255)
                                                                                                               tACGCCCTGAATCACCACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATgg                                   >  6:398508/1‑149 (MQ=255)
                                                                                                                 cGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGc                                  <  1:335449/148‑1 (MQ=255)
                                                                                                                      tGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCAc                            >  7:59141/1‑149 (MQ=255)
                                                                                                                         ataaaCACCATCGCAGGCAGCAAACCGTGGGTGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACttt                         <  4:373331/146‑1 (MQ=255)
                                                                                                                            aaCACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTtaa                       <  5:332328/148‑1 (MQ=255)
                                                                                                                                ccAGCGCAGGCAGCAAACCGTGGGGCAAGAACCCGCCATTATCGCTGATTAAATGAAAGCCAGTGGTCTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTATAATAAgc                  >  3:349665/1‑149 (MQ=255)
                                                                                                                                 cAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCCCGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAgcg                 >  2:265604/1‑149 (MQ=255)
                                                                                                                                 cAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAgcg                 >  2:225201/1‑149 (MQ=255)
                                                                                                                                    cgcAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCa               >  7:408966/1‑148 (MQ=255)
                                                                                                                                      caggcagCAAACCGGGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGCAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAAc            <  3:261379/149‑1 (MQ=255)
                                                                                                                                         gcagcaAACCGTGCGGGAAGTAGGCGCCATTATCCGTGTTTAAATGAAAGCCAGTGGTGTTGCGATCCTGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCTAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAg         <  4:349665/149‑1 (MQ=255)
                                                                                                                                               aaCCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTcc   <  4:230065/149‑1 (MQ=255)
                                                                                                                                                aCCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGTCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTACa  >  1:281105/1‑149 (MQ=255)
                                                                                                                                                aCCGCGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATgg                                   >  6:30282/1‑116 (MQ=255)
                                                                                                                                                aCCGCGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATgg                                   >  6:24614/1‑116 (MQ=255)
                                                                                                                                                aCCGCGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATgg                                   <  5:24614/116‑1 (MQ=255)
                                                                                                                                                aCCGCGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATgg                                   <  5:30282/116‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCA  >  NC_000913/1525183‑1525475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑