Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,797,774 | C→T | 6.8% | P57P (CCG→CCA) | pheT ← | phenylalanine tRNA synthetase, beta subunit |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,797,774 | 0 | C | T | 6.8% | 184.3 / 2.8 | 59 | P57P (CCG→CCA) | pheT | phenylalanine tRNA synthetase, beta subunit |
Reads supporting (aligned to +/- strand): ref base C (25/30); new base T (2/2); total (27/32) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01 |
CTTTAATTTTGAAATCACCCGGCAGAACAGCACCAATGGTCGCTACCGCTACACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAGTGATTTGATTTGCCAGCGCATCGCTATCAATCGCCGGGTTCACCCATTC > NC_000913/1797626‑1797917 | cTTTAATTTTGAAATCACCCGGCAGAACAGCACCAATGGTCGCTACCGCTACACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCATGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTc > 2:319235/1‑149 (MQ=255) tAATTTTGAAATCACCCGGCAGAACAGCACCAATGGTCGCTACCGCTACACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCg < 3:291864/147‑1 (MQ=255) aaTTTTGAAATCACCCGGCAGAACAGCACCAATGGTCGCTACCGCTACACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGa < 3:353425/148‑1 (MQ=255) ttttGAAATCACCCGGGAGAACAGCACCAATGGTCGCTACCGCTACACGCAGGCGCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGAtgc < 5:388522/149‑1 (MQ=255) gAAATCACCCGGCAGAACAGCACCAATGGTCGCTACGGCTACACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGAtgctgc < 7:117011/148‑1 (MQ=255) aaTCACCCGGCAGAACAGCACCAATGGTCGCTACCGCTACACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGca < 3:107224/149‑1 (MQ=255) tCACCCGGCAGAACAGCACCAATGGTCGCTACCGCTACACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGcaca < 3:384468/149‑1 (MQ=255) cccGGCAGAACAGCACCAATGGTCGCTACCGCTACACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTc < 1:319235/149‑1 (MQ=255) ccGGCAGAACAGCACCAATGGTCGCTACCGCTACACGCAGGCCCTGACGGCAGTTTGGCGCACCTCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCa < 5:229559/149‑1 (MQ=255) ggCAGAACAGCACCAATGGTCGCTACCGCTACACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAAc < 3:147053/149‑1 (MQ=255) aCCAATGGTCGCTACCGCTACACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCCCCaa > 3:61811/1‑149 (MQ=255) cAATGGTCGCTACCGCTACACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCAGTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGAACACGCCACCACTTCAcaacc > 5:175597/1‑144 (MQ=255) tGGTCGCTACCGCTACACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGACGCCGACATTCACTTTTGTCACAAGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTAACCAACGAcc > 8:155070/1‑149 (MQ=255) gTCGCTACCGCTACACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACTATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCa < 6:264657/148‑1 (MQ=255) gTCGCTACCGCTACACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCa < 8:105506/148‑1 (MQ=255) gctaccgctacACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGcc < 3:120969/149‑1 (MQ=255) gctaccgctacACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGcc > 4:207328/1‑149 (MQ=255) ctaccgctacACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGCCCTTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCCCCCACGTCCCCAACGCCCACGCCg > 1:90411/1‑149 (MQ=255) taccgctacACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGt > 7:367773/1‑149 (MQ=255) ctacACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAg < 5:251918/149‑1 (MQ=255) ctacACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAg < 6:50283/149‑1 (MQ=255) acGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGc < 8:325251/149‑1 (MQ=255) gCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGaa > 8:397592/1‑142 (MQ=255) gCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGaa < 7:397592/142‑1 (MQ=255) cccTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAAc < 3:207328/149‑1 (MQ=255) cTGATGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGCCATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCg > 4:345872/1‑149 (MQ=255) cTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAAGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCg < 5:363156/149‑1 (MQ=255) aCGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGtt < 1:298452/149‑1 (MQ=255) aCGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGt > 8:143696/1‑148 (MQ=255) aGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTaca < 7:143696/149‑1 (MQ=255) gTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTacac > 8:258423/1‑149 (MQ=255) gcgcACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTc > 4:260840/1‑148 (MQ=255) gcgcACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTc < 5:301263/148‑1 (MQ=255) cACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGATTTCGTTTTTGATGCTGCGTGCAACGTGTTCTACACCGTCAACt < 4:85068/149‑1 (MQ=255) aCGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCa < 5:300692/145‑1 (MQ=255) aCGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCa > 6:300692/1‑145 (MQ=255) tGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTTGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCa < 4:51135/141‑1 (MQ=255) tGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTTGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCa > 1:231061/1‑141 (MQ=255) tGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTTGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCa > 3:51135/1‑141 (MQ=255) tGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTTGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCa < 2:231061/141‑1 (MQ=255) cagcagGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCt < 1:166238/121‑1 (MQ=255) cagcagGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCt > 2:166238/1‑121 (MQ=255) cagcagGCGATCGCCGACGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAg < 5:13989/149‑1 (MQ=255) agcagGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAGt < 8:367773/149‑1 (MQ=255) tCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAGtgatttgatt > 3:172841/1‑149 (MQ=255) ccgccgACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAGTGATTTGATTTGc < 5:232676/149‑1 (MQ=255) ttCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAGTGATTTGATTTGCCAGCGCATc > 3:188459/1‑149 (MQ=255) cACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAGTGATTTGATTTGCCAGCGCATCGc > 6:10254/1‑149 (MQ=255) cACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAGTGATTTGATTTGCCAGCGCATCGc > 8:192472/1‑149 (MQ=255) cACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTGCTACACCGTCAACTTCCAGGCCCGCCATAGTGATTTGATTTGCCAGCGCATCg < 3:345872/148‑1 (MQ=255) aCTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAGTGATTTGATTTGCCAGCGCATCGc > 2:5445/1‑148 (MQ=255) aCTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAGTGATTTGATTTGCCAGCGCATCGCt < 4:61811/149‑1 (MQ=255) ttttGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAGTGATTTGATTTGCCAGCGCATCGCTAt > 7:151098/1‑149 (MQ=255) acacGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAGTGATTTGATTTGCTAGCGCATCGCTATCAATCGc > 2:215832/1‑149 (MQ=255) acGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAGTGATTTGATTTGCCAGCGCATCGCTATCAATCGCCg > 7:128880/1‑149 (MQ=255) acGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAGTGATTTGATTTGCCAGCGCATCGCTATCAATCGCCg > 4:207847/1‑149 (MQ=255) gCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAGTGATTTGATTTGCCAGCGCATCGCTATCAATCGCCggg > 8:316844/1‑149 (MQ=255) gTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACCCCGTCAACTTCCAGGCCCGCCATAGTGATTTGATTTGCCAGCGCATCGCTATCAATCGCCGGGTTCAcc > 1:22758/1‑148 (MQ=255) tCAGCGTTCGGCTGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTTGAAGCTGCCTGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAGTGATTTGATTTGCCAGCGCATCGCTATCAATCGCCGGGTTCAccc < 4:197958/148‑1 (MQ=255) gCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGCCCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAGTGATTTGATTAGCCAGCGCCACGCTATCACTCGCCGGGGTCACCCAtgc > 6:108977/1‑147 (MQ=255) | CTTTAATTTTGAAATCACCCGGCAGAACAGCACCAATGGTCGCTACCGCTACACGCAGGCCCTGACGGCAGTTTGGCGCACCGCAGACGATGTCCAGCAGGCGATCGCCGCCGACATTCACTTTTGTCACACGCAGTTTGTCAGCGTTCGGATGCTGCGCACACTCAACCACTTCACCAACGACCACGCCGTGGAAGCTGCCGGCAACCGGTTCTACACCGTCAACTTCCAGGCCCGCCATAGTGATTTGATTTGCCAGCGCATCGCTATCAATCGCCGGGTTCACCCATTC > NC_000913/1797626‑1797917 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |