Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,550,064 T→C 5.5% K161R (AAA→AGA)  yfeX ← porphyrinogen oxidase, cytoplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,550,0640TC 5.5% 225.9 / 3.3 72K161R (AAA→AGA) yfeXporphyrinogen oxidase, cytoplasmic
Reads supporting (aligned to +/- strand):  ref base T (38/30);  new base C (2/2);  total (40/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

TCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCACTTCAATGCAGTCACCAAAGGCTTCCATCGCCG  >  NC_000913/2549920‑2550211
                                                                                                                                                |                                                                                                                                                   
tcgtcgCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACCTAGCTGCCGCCCGCATCCACGCCGTCTTTGa                                                                                                                                                  >  5:367015/1‑148 (MQ=255)
   tcgCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCGCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCTCGCCGTCTTTGATAAc                                                                                                                                              <  6:307152/149‑1 (MQ=255)
        ggcGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTCAGCTGCTTCAGGTTGTGTTCCCAACGCGGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCa                                                                                                                                         <  5:98473/147‑1 (MQ=255)
            aTCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTc                                                                                                                                     >  8:145986/1‑149 (MQ=255)
                 ttCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGAc                                                                                                                                >  4:181728/1‑149 (MQ=255)
                     ttGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGt                                                                                                                            >  3:343161/1‑149 (MQ=255)
                        gCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCCGCTTCAGGTTGTGTTCCCAACGCTGGACAACCACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGtctc                                                                                                                         <  4:306039/149‑1 (MQ=255)
                           tctTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCa                                                                                                                                         >  6:96637/1‑130 (MQ=255)
                           tctTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCa                                                                                                                                         <  5:96637/130‑1 (MQ=255)
                             tttGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCAc                                                                                                                    >  7:4628/1‑149 (MQ=255)
                              ttGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTCTGATAACCGCCACTTCGCGACGCGTCTCTTCAcc                                                                                                                   >  2:204908/1‑149 (MQ=255)
                               tGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCAc                                                                                                                    <  3:28424/147‑1 (MQ=255)
                               tGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGGGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCAcc                                                                                                                   <  2:240237/148‑1 (MQ=255)
                                  tGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATTCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACccgcc                                                                                                               >  8:257205/1‑149 (MQ=255)
                                          aTCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTc                                                                                                       >  6:6384/1‑149 (MQ=255)
                                          aTCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGACGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGtct                                                                                                                          <  6:204324/130‑1 (MQ=255)
                                          aTCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGACGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGtct                                                                                                                          >  5:204324/1‑130 (MQ=255)
                                           tCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTcc                                                                                                      <  8:4628/149‑1 (MQ=255)
                                             aCCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGc                                                                                                                                           <  4:68604/110‑1 (MQ=255)
                                             aCCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGc                                                                                                                                           >  3:68604/1‑110 (MQ=255)
                                               cATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTAc                                                                                                  >  6:66162/1‑149 (MQ=255)
                                               cATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTAc                                                                                                  >  2:253418/1‑149 (MQ=255)
                                               cATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTAc                                                                                                  >  7:149343/1‑149 (MQ=255)
                                               cATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTCTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTAc                                                                                                  <  1:204908/149‑1 (MQ=255)
                                                     cTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTc                                                                                              <  5:276315/147‑1 (MQ=255)
                                                       gATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTcaac                                                                                           >  7:364610/1‑148 (MQ=255)
                                                        aTCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTcaaca                                                                                          >  2:293432/1‑148 (MQ=255)
                                                         tCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTcaaca                                                                                          >  5:123515/1‑147 (MQ=255)
                                                           gTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAg                                                                                       <  4:97021/148‑1 (MQ=255)
                                                           gTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGc                                                                                      <  4:153012/149‑1 (MQ=255)
                                                            tGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGcc                                                                                     >  8:378747/1‑149 (MQ=255)
                                                                 gCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCt                                                                                  >  6:195021/1‑147 (MQ=255)
                                                                 gCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCa                                                                                <  7:43001/149‑1 (MQ=255)
                                                                   tCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTCTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAgg                                                                              >  3:379595/1‑149 (MQ=255)
                                                                     aTCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATTCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAgg                                                                              <  7:257205/147‑1 (MQ=255)
                                                                     aTCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGt                                                                             >  4:179719/1‑148 (MQ=255)
                                                                     aTCCGGTTGAGCTGCTTCAGGTTGTGGTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTc                                                                            <  8:104563/149‑1 (MQ=255)
                                                                         ggTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGc                                                                        >  3:176160/1‑149 (MQ=255)
                                                                         ggTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGc                                                                        >  7:206447/1‑149 (MQ=255)
                                                                             gAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGctct                                                                     <  7:145986/148‑1 (MQ=255)
                                                                               gctgctTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATATCCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCa                                                                  <  2:54117/149‑1 (MQ=255)
                                                                                     tCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCaa                                                            >  2:228528/1‑149 (MQ=255)
                                                                                         gTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACgg                                                         >  7:386394/1‑148 (MQ=255)
                                                                                            gtgtTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGc                                                     >  7:20909/1‑149 (MQ=255)
                                                                                                  ccAACGCTGGACAAACACATAGCTGCCGCCCGCATCAACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGa                                               >  8:110756/1‑149 (MQ=255)
                                                                                                  ccAACGCTGGACAAACACATAGCTGCCGCCCGCATCAACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTATCTTCACCAGCCGGGTTTTCCGTACCGTCAAAAAAGCCGCTCAGTTAACGCTCTTCAACCCAACGGAAGCCGAGGa                                               >  2:324013/1‑149 (MQ=255)
                                                                                                   cAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGAt                                              >  8:138388/1‑149 (MQ=255)
                                                                                                      cGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCGACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCTTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGAtctc                                           <  6:48073/149‑1 (MQ=255)
                                                                                                       gCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATctct                                          <  6:123515/149‑1 (MQ=255)
                                                                                                       gCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATctct                                          <  1:279714/149‑1 (MQ=255)
                                                                                                            aCAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCACCCCAACGGAAGCCGTGGATCTCTTCttt                                     >  2:279678/1‑149 (MQ=255)
                                                                                                            aCAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCttt                                     <  8:20909/149‑1 (MQ=255)
                                                                                                              aaaCACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCa                                   >  4:104464/1‑149 (MQ=255)
                                                                                                              aaaCACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCa                                   <  5:6384/149‑1 (MQ=255)
                                                                                                               aaCACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCAc                                  >  7:98157/1‑149 (MQ=255)
                                                                                                                 cacaTAGCTGCCGCCCGCATCCACGCCGTCTCTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCACtt                                <  4:379595/149‑1 (MQ=255)
                                                                                                                  acaTAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCACtt                                >  6:273126/1‑148 (MQ=255)
                                                                                                                  acaTAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCACtt                                >  7:132958/1‑148 (MQ=255)
                                                                                                                       gCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCACTTCAATg                           <  1:293432/148‑1 (MQ=255)
                                                                                                                       gCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCACTTCAATg                           <  4:343161/148‑1 (MQ=255)
                                                                                                                       gCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCACTTCAATg                           >  3:314420/1‑148 (MQ=255)
                                                                                                                           ccgcccgcATCCACGCCGGCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCACTTCAATGCAg                        <  8:149343/147‑1 (MQ=255)
                                                                                                                            cgcccgcATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCACTTCAATGCAGTCa                     <  8:132958/149‑1 (MQ=255)
                                                                                                                             gcccgcATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCACTTCAATGCAGTCAc                    <  3:104464/149‑1 (MQ=255)
                                                                                                                               ccgcATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTg                                                 <  5:23952/118‑1 (MQ=255)
                                                                                                                               ccgcATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTg                                                 >  6:23952/1‑118 (MQ=255)
                                                                                                                                cgcATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTg                                                 <  1:347996/117‑1 (MQ=255)
                                                                                                                                cgcATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTg                                                 >  2:347996/1‑117 (MQ=255)
                                                                                                                                      cccGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCACTTCAATGCAGTCACCAAAGGCtt           <  1:253418/147‑1 (MQ=255)
                                                                                                                                      catgCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCACCGGAAGCCGTGGATCTCTTCTTTCACTTCAATGCAGTCACCAAAGGCtt           >  2:309845/4‑149 (MQ=255)
                                                                                                                                       aCGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCACTTCAATGCAGTCACCAAAGGCTTc          >  5:161936/1‑149 (MQ=255)
                                                                                                                                           cGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCACTTCAATGCAGTCACCAAAGGCTTCCATc      >  5:82063/1‑149 (MQ=255)
                                                                                                                                               tttGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCACTTCAATGCAGTCACCAAAGGCTTCCATcgccg  >  2:197889/1‑149 (MQ=255)
                                                                                                                                               tttGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCAGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCCCTTCAATGCAGTCACCAAAGGCATCCAAcgccg  >  3:281284/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGCATCCACGCCGTCTTTGATAACCGCCACTTCGCGACGCGTCTCTTCACCCGCCGGGTTTTCCGTACCGTCAACAAAGCCGCTCAGGTCACGCTCTTCAACCCAACGGAAGCCGTGGATCTCTTCTTTCACTTCAATGCAGTCACCAAAGGCTTCCATCGCCG  >  NC_000913/2549920‑2550211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑