Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,231,792 T→C 6.4% F43S (TTT→TCT)  fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,231,7920TC 6.4% 190.2 / 3.7 62F43S (TTT→TCT) fadH2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base T (25/33);  new base C (2/2);  total (27/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

TACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTA  >  NC_000913/3231647‑3231937
                                                                                                                                                 |                                                                                                                                                 
tACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGtttt                                                                                                                                                 <  3:345248/148‑1 (MQ=255)
 aCATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTTGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTa                                                                                                                                                <  2:323687/148‑1 (MQ=255)
 aCATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTa                                                                                                                                                <  1:339561/148‑1 (MQ=255)
    tACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGcc                                                                                                                                            >  3:145567/1‑149 (MQ=255)
     aCTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGcc                                                                                                                                            >  1:85837/1‑148 (MQ=255)
      cTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCg                                                                                                                                           >  2:273096/1‑148 (MQ=255)
            aaTTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACgcgc                                                                                                                                    <  3:8917/149‑1 (MQ=255)
                     aGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACAGCGTGTTGATTGTCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGAGTTTTATGCCGAACGCGCCCGTCACgg                                                                                                                           <  4:29394/149‑1 (MQ=255)
                      gCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGc                                                                                                                          >  3:146321/1‑149 (MQ=255)
                      gCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGc                                                                                                                          <  8:30957/149‑1 (MQ=255)
                          cccGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGAGTTTTATGCCGAACGCGCCCGTCACGGCGTgg                                                                                                                      >  8:188200/1‑149 (MQ=255)
                             gTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTa                                                                                                                                                <  2:237715/120‑1 (MQ=255)
                             gTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTa                                                                                                                                                >  1:237715/1‑120 (MQ=255)
                             gTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGgcgc                                                                                                                   >  2:323394/1‑149 (MQ=255)
                                gCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGCTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGa                                                                                                                <  3:251095/149‑1 (MQ=255)
                                   gTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTg                                                                                                             >  3:114966/1‑149 (MQ=255)
                                         cccGCTGGATTTCGGTGTTACCACGTTAAAACACCGCGGGTTGATGGGCTCCATGCCCACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTCTGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAgcg                                                                                                       <  5:307446/149‑1 (MQ=255)
                                                gATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCggcggc                                                                                                  <  2:85837/147‑1 (MQ=255)
                                                 aTTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGTATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCAtt                                                                                               <  8:358343/149‑1 (MQ=255)
                                                   ttAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTg                                                                                              >  7:15116/1‑148 (MQ=255)
                                                       gTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCa                                                                                         <  5:275183/149‑1 (MQ=255)
                                                       gTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTCTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCAc                                                                                           >  5:288298/1‑147 (MQ=255)
                                                       gTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTCTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCAc                                                                                           >  5:293748/1‑147 (MQ=255)
                                                          ttACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGAt                                                                                      >  5:67282/1‑149 (MQ=255)
                                                             ccACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGAttt                                                                                    >  8:306283/1‑148 (MQ=255)
                                                              cACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTaa                                                                                  >  1:265314/1‑149 (MQ=255)
                                                              cACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTaa                                                                                  <  4:266632/149‑1 (MQ=255)
                                                                cGTTAAATAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACa                                                                                <  7:306283/149‑1 (MQ=255)
                                                                    aaaaaaCCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGAtt                                                                                                              >  7:64547/1‑115 (MQ=255)
                                                                    aaaaaaCCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGAtt                                                                                                              <  8:64547/115‑1 (MQ=255)
                                                                       aaaCCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTg                                                                         <  6:67282/149‑1 (MQ=255)
                                                                            gcgTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATg                                                                    <  4:73559/149‑1 (MQ=255)
                                                                               tgtTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATgg                                                                   >  1:334815/1‑147 (MQ=255)
                                                                               tgtTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATgg                                                                   >  1:321376/1‑147 (MQ=255)
                                                                                gtTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAg                                                                >  2:378630/1‑149 (MQ=255)
                                                                                         cTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCaa                                                       <  2:334815/149‑1 (MQ=255)
                                                                                            aaTGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAAt                                                      <  2:265314/147‑1 (MQ=255)
                                                                                            aaTGCACACCGGGCGGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAAt                                                      <  4:161073/147‑1 (MQ=255)
                                                                                                   acCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAacga                                             <  7:129115/149‑1 (MQ=255)
                                                                                                   acCGGGCTGGAGGAATACACGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCTCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTTGCATGGAAGGTGGCGCAATGCTCAacga                                             <  4:68105/149‑1 (MQ=255)
                                                                                                    ccGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAccgac                                            >  5:112087/1‑149 (MQ=255)
                                                                                                     cGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAacgacg                                           >  7:406926/1‑149 (MQ=255)
                                                                                                      gggCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACgc                                          <  1:378630/149‑1 (MQ=255)
                                                                                                       ggCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACgcc                                         <  8:48085/149‑1 (MQ=255)
                                                                                                            gaggaATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACgccagcc                                     <  1:154550/148‑1 (MQ=255)
                                                                                                            gaggaATACCCGGACGGTGCCGAGCGGCTGGCAGCGTCTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACgccagcc                                     <  6:288298/148‑1 (MQ=255)
                                                                                                            gaggaATACCCGGACGGTGCCGAGCGGCTGGCAGCGTCTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACgccagcc                                     <  6:293748/148‑1 (MQ=255)
                                                                                                              ggaATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACgccagc                                      >  5:169884/1‑145 (MQ=255)
                                                                                                                  tACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCa                                                                      <  6:329283/109‑1 (MQ=255)
                                                                                                                  tACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCa                                                                      >  5:329283/1‑109 (MQ=255)
                                                                                                                  tACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATccc                              <  4:146321/149‑1 (MQ=255)
                                                                                                                     ccggccggTGCCGAGCGGCTGGCACAGTTTTATGCCGAACGCGCACGTCACGGCGTGGCGCTTATTGTCAGCGGCGGCATAGCACCAGATTAAACAGGCGGTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCcacc                           >  2:303223/1‑148 (MQ=255)
                                                                                                                     ccGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCcaca                           >  1:260239/1‑149 (MQ=255)
                                                                                                                         aCGGTGCCGAGCGGCGGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCAt                       <  5:212875/149‑1 (MQ=255)
                                                                                                                            gTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGc                    >  3:73816/1‑149 (MQ=255)
                                                                                                                                cGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCa                <  2:260239/149‑1 (MQ=255)
                                                                                                                                cGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCa                <  1:226721/149‑1 (MQ=255)
                                                                                                                                    cGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTAc            <  1:323394/149‑1 (MQ=255)
                                                                                                                                    cGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTAc            <  1:273096/149‑1 (MQ=255)
                                                                                                                                    cGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTAc            <  6:166440/149‑1 (MQ=255)
                                                                                                                                        tGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCg          >  7:267730/1‑147 (MQ=255)
                                                                                                                                           cAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCg     >  1:263687/1‑149 (MQ=255)
                                                                                                                                              cGTTTTATGCCGAACGCGCACGTCACGGCTGGGCGCTGATTGTCAGCGGCGTCATTGCACCAGATTTAACAGGCGTTGGCATCGAAGGAGGCGCAATGCTCAACGACGCCAGCCAGTTCCCACACCTTCGCACCATTACCGTAGCGGTa  <  6:324217/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTA  >  NC_000913/3231647‑3231937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑