Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,935,337 C→A 8.1% W326C (TGG→TGT zwf ← glucose‑6‑phosphate 1‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,935,3370CA 8.1% 244.1 / 7.4 76W326C (TGG→TGTzwfglucose‑6‑phosphate 1‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (32/36);  new base A (2/4);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

GACGGATAGTCAGTTTATTCTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTT  >  NC_000913/1935189‑1935483
                                                                                                                                                    |                                                                                                                                                  
gACGGATAGTCAGTTTATTCTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAgcgc                                                                                                                                                    <  2:10847/149‑1 (MQ=255)
  cGGATAGTCAGTTTATTCTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGGTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCa                                                                                                                                                  <  1:387169/149‑1 (MQ=255)
        gTCAGTTTATTCTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTAt                                                                                                                                             <  6:257014/148‑1 (MQ=255)
        gTCAGTTTATTCTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTAt                                                                                                                                             <  1:180216/148‑1 (MQ=255)
         tCAGTTTATTCTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATc                                                                                                                                            >  8:155472/1‑148 (MQ=255)
           aGTTTATTCTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAAt                                                                                                                                         <  6:347832/149‑1 (MQ=255)
             tttATTCTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGt                                                                                                                                       <  8:253407/149‑1 (MQ=255)
             tttATTCTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGt                                                                                                                                       >  7:200524/1‑149 (MQ=255)
             tttATTCTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGt                                                                                                                                       >  5:340955/1‑149 (MQ=255)
                            aTCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCa                                                                                                                        >  1:125829/1‑149 (MQ=255)
                                 gCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGa                                                                                                                     >  3:35934/1‑147 (MQ=255)
                                  ccACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGACAGTTATCAATGTCGACGCGGATCGCCACGAAAg                                                                                                                  >  2:17462/1‑149 (MQ=255)
                                     cGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGttt                                                                                                               <  3:262825/149‑1 (MQ=255)
                                     cGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGttt                                                                                                               <  2:389863/149‑1 (MQ=255)
                                       aTTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGACAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCt                                                                                                             <  7:327882/149‑1 (MQ=255)
                                         tCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTg                                                                                                            >  5:268949/1‑148 (MQ=255)
                                          cTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGt                                                                                                           <  8:99442/148‑1 (MQ=255)
                                           tttAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTAt                                                                                                         >  5:178727/1‑149 (MQ=255)
                                           tttAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTAt                                                                                                         >  2:289315/1‑149 (MQ=255)
                                             tAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCt                                                                                                             >  2:306010/1‑143 (MQ=255)
                                             tAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCt                                                                                                             <  1:306010/143‑1 (MQ=255)
                                             tAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTa                                                                                                          <  3:104635/146‑1 (MQ=255)
                                             tAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTAt                                                                                                         <  4:35934/147‑1 (MQ=255)
                                               aaCAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATtgc                                                                                                      <  7:5884/148‑1 (MQ=255)
                                                aCAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCa                                                                                                                                                  >  3:175920/1‑103 (MQ=255)
                                                aCAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCa                                                                                                                                                  <  4:175920/103‑1 (MQ=255)
                                                aCAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGCCGCGGATCGCCACGAAAGTTTCTGTATtgctg                                                                                                    >  2:193214/1‑149 (MQ=255)
                                                  aGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTgctgct                                                                                                  >  5:334322/1‑149 (MQ=255)
                                                         gttcagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTc                                                                                           <  7:155472/149‑1 (MQ=255)
                                                          ttcagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTc                                                                                           <  2:180926/148‑1 (MQ=255)
                                                          ttcagGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTc                                                                                           >  1:180926/1‑148 (MQ=255)
                                                                 gtTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATtgc                                                                                                      >  4:75725/1‑130 (MQ=255)
                                                                 gtTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATtgc                                                                                                      <  3:75725/130‑1 (MQ=255)
                                                                      gAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTcgcg                                                                                        >  6:323686/1‑139 (MQ=255)
                                                                      gAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTcgcg                                                                                        <  5:323686/139‑1 (MQ=255)
                                                                      gAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCtcttct                                                                               <  6:334322/148‑1 (MQ=255)
                                                                       aaaTAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCttcttc                                                                             >  4:329827/1‑149 (MQ=255)
                                                                       aaaTAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCttcttc                                                                             <  1:245242/149‑1 (MQ=255)
                                                                        aaTAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTcc                                                                            <  3:234761/149‑1 (MQ=255)
                                                                         aTAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCa                                                                           <  2:355909/149‑1 (MQ=255)
                                                                         aTAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCa                                                                           <  3:222849/149‑1 (MQ=255)
                                                                             aCCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGata                                                                       >  8:383379/1‑149 (MQ=255)
                                                                              ccACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGatat                                                                      >  5:163930/1‑149 (MQ=255)
                                                                               cACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCt                                                                                                             >  6:260695/1‑109 (MQ=255)
                                                                               cACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCt                                                                                                             <  5:260695/109‑1 (MQ=255)
                                                                               cACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGACAGtt                                                                                                                                               <  3:142082/75‑1 (MQ=255)
                                                                               cACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGACAGtt                                                                                                                                               >  4:142082/1‑75 (MQ=255)
                                                                                  gACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCg                                                                  <  1:289315/149‑1 (MQ=255)
                                                                                     ttCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCa                                                               >  4:17880/1‑149 (MQ=255)
                                                                                     ttCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCa                                                               >  4:22841/1‑149 (MQ=255)
                                                                                        aGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGctc                                                                                                 >  6:159689/1‑112 (MQ=255)
                                                                                        aGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGctc                                                                                                 <  5:159689/112‑1 (MQ=255)
                                                                                         gAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACttt                                                           >  7:361130/1‑149 (MQ=255)
                                                                                          aaCATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACtttt                                                          <  3:329827/149‑1 (MQ=255)
                                                                                          aaCATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACtttt                                                          >  4:91837/1‑149 (MQ=255)
                                                                                          aaCATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCACCGACAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCGCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACtttt                                                          <  6:94079/149‑1 (MQ=255)
                                                                                           aCATTTGGTCGGCAGACGGTTACCAGTACGCAGGTAGAAGGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACttttt                                                         <  6:20199/149‑1 (MQ=255)
                                                                                               ttGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGGCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGcc                                                     >  5:383671/1‑149 (MQ=255)
                                                                                                   tCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTgg                                                 <  2:125829/149‑1 (MQ=255)
                                                                                                        aGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCg                                              >  4:321759/1‑147 (MQ=255)
                                                                                                        aGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGaa                                            <  3:321759/149‑1 (MQ=255)
                                                                                                                aCCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACtttttt                                                        >  6:281045/1‑129 (MQ=255)
                                                                                                                aCCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACtttttt                                                        <  5:281045/129‑1 (MQ=255)
                                                                                                                 ccAGTACGCAGGTAGAATGGCACACCGGCCCAGCGGCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGt                                   <  6:383671/149‑1 (MQ=255)
                                                                                                                  cAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGta                                  >  6:167858/1‑149 (MQ=255)
                                                                                                                    gTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGtata                                <  8:361130/149‑1 (MQ=255)
                                                                                                                        ggaGGTAGAATGGCCCACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCACTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGc                            <  5:216881/147‑1 (MQ=255)
                                                                                                                           ggTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCcg                         >  6:61479/1‑149 (MQ=255)
                                                                                                                                aaTGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTAc                    <  3:91837/149‑1 (MQ=255)
                                                                                                                                aaTGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCCCGTAc                    <  6:369956/149‑1 (MQ=255)
                                                                                                                                 aTGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACg                   <  3:17880/149‑1 (MQ=255)
                                                                                                                                 aTGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACg                   <  3:22841/149‑1 (MQ=255)
                                                                                                                                       cacCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGttttt             >  6:14860/1‑149 (MQ=255)
                                                                                                                                        acCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGtttttt            >  1:313661/1‑149 (MQ=255)
                                                                                                                                        acCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTCCCCGCGTACGGtttttt            >  6:57436/1‑149 (MQ=255)
                                                                                                                                         ccGGCCCAGCGACAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTc           <  1:17462/149‑1 (MQ=255)
                                                                                                                                               cAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTAc     >  1:18252/1‑149 (MQ=255)
                                                                                                                                                  ccccAGTTATCACTGCCGACGCCCATCGCCACGAACGATGCAGCATTGCCGCTCTTCTTCGCGCCCCCTGCTTCCACATCTCCCGGCACCTGTTTGCCCGGGGCGAAGCCCGCAGTATATTGCCCGCGAACGGTTTTTTCGCCTACggt  <  6:173485/147‑3 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GACGGATAGTCAGTTTATTCTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTT  >  NC_000913/1935189‑1935483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: