Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,299,544 G→A 8.3% A12V (GCG→GTG)  napH ← ferredoxin‑type protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,299,5440GA 8.3% 141.9 / 3.2 48A12V (GCG→GTG) napHferredoxin‑type protein
Reads supporting (aligned to +/- strand):  ref base G (22/22);  new base A (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

AATAACAGGCTACTGCTGTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTCCAGCACGCATACTTTTTCGCAC  >  NC_000913/2299400‑2299688
                                                                                                                                                |                                                                                                                                                
aaTAACAGGCTACTGCTGTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGCCAAAGGCGACGTAACACCAGCCACCGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTc                                                                                                                                              >  2:363969/1‑149 (MQ=255)
  tAACAGGCTACTGCTGTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTcgc                                                                                                                                            <  8:89977/149‑1 (MQ=255)
        gCTACTGCTGTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCgg                                                                                                                                      >  2:229234/1‑149 (MQ=255)
               ctgTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACg                                                                                                                               >  4:357043/1‑149 (MQ=255)
                tgTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCCCCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGt                                                                                                                              >  4:76146/1‑149 (MQ=255)
                  ttgTTGCCGGGGAAGATCTACACAACAATCCATGGACCGCTCAAAAACATACCCAGCAGGAATTTGTGACAAAGGAGTCGTAACTGCAGCCTACGGTGACTGGGCCTCCAGGCTTTTTTCTGCAGCGCCTCGCGCCAGGCGTCTCGtt                                                                                                                             <  6:217640/146‑1 (MQ=255)
                         cGTCCAAGATACACACACCAAACCATGGACCGCTCAAAAACATCCACAGCACGAAGAACTGACAAACGCGACGTAACACCAGCCAACGGTGACTGCGCCACCCGCCTTATTTCTCCAGCGCCTCGCCCCCGGCCTCACGATTACCAt                                                                                                                       >  2:273262/1‑147 (MQ=255)
                           tGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGc                                                                                                                   <  8:334398/149‑1 (MQ=255)
                           tGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCACCTCGCGCCCGGCGTCACGTTTACGa                                                                                                                        >  4:210369/1‑144 (MQ=255)
                           tGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCACCTCGCGCCCGGCGTCACGTTTACGa                                                                                                                        <  3:210369/144‑1 (MQ=255)
                           tGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGACACCAGCCTTTTTTCTCCAGCACCTCGCGCCCGGCGTCACGTTTACGATTTGc                                                                                                                   >  4:250175/1‑149 (MQ=255)
                             cAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGACCTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCCCCAGCCGTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGGTTACGATTTGCCa                                                                                                                 >  1:114411/1‑149 (MQ=255)
                                  tCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGtt                                                                                                            <  8:349444/149‑1 (MQ=255)
                                      cacacCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACGGCCAAAGGCGCCGTAACCCCAGCCAACGGTGACTGCGCCACCAGCCGTTTTTCTCCAGCGCCTCGCGCCCGGCGTCCCGTTTACGATTTGCGATTGTTcccc                                                                                                        >  2:184225/1‑149 (MQ=255)
                                           cAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTa                                                                                                                           <  5:374062/125‑1 (MQ=255)
                                           cAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTa                                                                                                                           >  6:374062/1‑125 (MQ=255)
                                           cAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTc                                                                                                      <  8:49431/146‑1 (MQ=255)
                                           cAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTc                                                                                                      >  7:49431/1‑146 (MQ=255)
                                               ccATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCACCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTccagccag                                                                                               <  3:250175/149‑1 (MQ=255)
                                                      ccGCTCAAAAACATCCCCAGCACGAAGACCTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGc                                                                                        <  4:358767/149‑1 (MQ=255)
                                                         cTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCCCCCGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGGCCCGTTTACGATGTGCCATTGTTCCCCTCCAGCCCGCCGACGCgg                                                                                      >  4:186206/1‑148 (MQ=255)
                                                          tCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGt                                                                                     >  4:174287/1‑148 (MQ=255)
                                                              aaaCATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATgg                                                                                <  3:76146/149‑1 (MQ=255)
                                                                  aTCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGAc                                                                            <  1:229234/149‑1 (MQ=255)
                                                                        aGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACt                                                                      <  8:22272/149‑1 (MQ=255)
                                                                           aCGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTc                                                                     <  8:382111/147‑1 (MQ=255)
                                                                           aCGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTc                                                                     >  7:382111/1‑147 (MQ=255)
                                                                                gaaCTGACAAAGGCGAGGTAACACCAGCGTACGGTTACTGCGCCACCAGCGTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCATCCAGCCGAAGCGGTAATGGTGACCTAACTCGCCTTTc                                                              <  1:273262/149‑1 (MQ=255)
                                                                                        aaaGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTCTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGa                                                      >  8:380370/1‑149 (MQ=255)
                                                                                          aGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGa                                                      >  5:360162/1‑147 (MQ=255)
                                                                                                  taccaccacCCACAGGTGACTGCGCCACCAGCCTTTTTTCACCAGCGCCTCGCGCCCGTCGTCACGTATACGATTTGCCATTGTTCCCCTCCCGCCAGCCGAAGCGGGAATGGTGACCTAACTCCCCATTCGCCAGTGACAGCGGTAac                                            <  6:284280/146‑1 (MQ=255)
                                                                                                    acacCAGCCAACGGTGACTGCGACACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCTGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCGAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCa                                                          <  5:91261/133‑1 (MQ=255)
                                                                                                       ccAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCtt                                       <  6:360162/149‑1 (MQ=255)
                                                                                                           ccAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGAtt                                   >  6:270531/1‑149 (MQ=255)
                                                                                                               cGGGGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCACTCCAGCCCGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCg                               <  4:331891/149‑1 (MQ=255)
                                                                                                                    aCTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTTATTGCCGgttgt                          >  8:95345/1‑149 (MQ=255)
                                                                                                                        cgcCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTc                        <  3:174287/147‑1 (MQ=255)
                                                                                                                           caccaGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTCCAGCa                   >  2:53846/1‑149 (MQ=255)
                                                                                                                              caGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTCCAGCACg                 >  6:136416/1‑148 (MQ=255)
                                                                                                                                  cTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTCCAGCACGCATAc            >  3:162174/1‑149 (MQ=255)
                                                                                                                                  cTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTCCAGCACGCATAc            <  1:53846/149‑1 (MQ=255)
                                                                                                                                   ttttttCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTCCAGCACGCATACt           >  8:53979/1‑149 (MQ=255)
                                                                                                                                        tctcCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTCCAGCACGCATACttttt       <  2:114411/148‑1 (MQ=255)
                                                                                                                                        tctcCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTCCAGCACGCATACttttt       <  5:270531/148‑1 (MQ=255)
                                                                                                                                         ctcCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTCCAGCACGCATACTTTTTc      <  1:363969/148‑1 (MQ=255)
                                                                                                                                            cAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTcccc                                                                  <  3:136681/85‑1 (MQ=255)
                                                                                                                                            cAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTcccc                                                                  >  4:136681/1‑85 (MQ=255)
                                                                                                                                            cAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTCCAGCACGCATACTTTTTCGCAc  >  8:116547/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AATAACAGGCTACTGCTGTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTCCAGCACGCATACTTTTTCGCAC  >  NC_000913/2299400‑2299688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑