Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,261,474 C→A Q134H (CAG→CAT prs ← phosphoribosylpyrophosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,261,4740CA95.7% 305.4 / 4.3 92Q134H (CAG→CATprsphosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (45/43);  total (47/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCGG  >  NC_000913/1261331‑1261619
                                                                                                                                               |                                                                                                                                                 
ttAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCCTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGtt                                                                                                                                               >  7:256802/1‑148 (MQ=255)
           gcgGGCACGCACAACGCCGCCGATGTTCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGAt                                                                                                                                   <  6:574893/149‑1 (MQ=255)
              ggCACGCACAACGCCGCCGATGTCCGGGGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCa                                                                                                                                <  6:195740/149‑1 (MQ=255)
              ggCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCa                                                                                                                                >  7:90420/1‑149 (MQ=255)
                   gcacAACGCCGCCGATGTCCGGAGAAGCCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGt                                                                                                                            >  3:365222/1‑148 (MQ=255)
                   gcacAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGt                                                                                                                            >  5:46244/1‑148 (MQ=255)
                   gcacAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTc                                                                                                                           <  8:218739/149‑1 (MQ=255)
                    cacaACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAACCCCTGAATATGTTCAGCGTGCAGATCCACTGTCa                                                                                                                          <  3:352428/149‑1 (MQ=255)
                     acaACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAATGTCGAAGAAACCCTGAATATGTTCa                                                                                                                                             <  7:212828/129‑1 (MQ=255)
                     acaACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAATGTCGAAGAAACCCTGAATATGTTCa                                                                                                                                             >  8:212828/1‑129 (MQ=255)
                       aaCGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGca                                                                                                                       >  8:458283/1‑149 (MQ=255)
                          gccgccGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCacac                                                                                                                    >  2:491851/1‑149 (MQ=255)
                                gATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCaa                                                                                                              <  7:94662/149‑1 (MQ=255)
                                    tCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACAcc                                                                                                          >  8:360729/1‑149 (MQ=255)
                                       ggAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGt                                                                                                                 <  8:184512/139‑1 (MQ=255)
                                       ggAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGt                                                                                                                 >  7:184512/1‑139 (MQ=255)
                                        gagaAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAAAACGTTATCAACCGGAACGTCGAAGAAACCCAGAATATGTTCAGCGTGCAGATACAATGTCAGCACACGGTCAACACCGACg                                                                                                      >  6:69822/1‑149 (MQ=255)
                                          gaAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGc                                                                                                     <  8:298692/148‑1 (MQ=255)
                                          gaAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGc                                                                                                     >  7:298692/1‑148 (MQ=255)
                                            aaCCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTg                                                                                                   >  3:462390/1‑148 (MQ=255)
                                             aCCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACt                                                                                                                              >  5:102957/1‑120 (MQ=255)
                                             aCCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACt                                                                                                                              <  6:102957/120‑1 (MQ=255)
                                             aCCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTc                                                                                                                <  3:362529/134‑1 (MQ=255)
                                             aCCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTc                                                                                                                >  4:362529/1‑134 (MQ=255)
                                             aCCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTg                                                                                                   >  1:510446/1‑147 (MQ=255)
                                               cacaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACgg                                                                                                                  >  7:629822/1‑130 (MQ=255)
                                               cacaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACgg                                                                                                                  <  8:629822/130‑1 (MQ=255)
                                               cacaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGgaga                                                                                               <  5:549318/149‑1 (MQ=255)
                                               cacaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGga                                                                                                 >  4:166551/1‑147 (MQ=255)
                                               cacaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGga                                                                                                 >  6:549318/1‑147 (MQ=255)
                                                acaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGagag                                                                                              <  3:345467/149‑1 (MQ=255)
                                                  aaTTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTc                                                                                                                <  5:405383/129‑1 (MQ=255)
                                                  aaTTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTc                                                                                                                >  6:405383/1‑129 (MQ=255)
                                                   aTTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGaa                                                                                           <  7:360729/149‑1 (MQ=255)
                                                    ttGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGACCGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAg                                                                                          >  2:202460/1‑149 (MQ=255)
                                                       ggTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCt                                                                                       <  6:46244/149‑1 (MQ=255)
                                                         ttATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGc                                                                                     <  8:90420/149‑1 (MQ=255)
                                                             ccAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCa                                                                                                                          <  7:601100/108‑1 (MQ=255)
                                                             ccAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCa                                                                                                                          >  8:601100/1‑108 (MQ=255)
                                                                gATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACt                                                                              >  5:317312/1‑149 (MQ=255)
                                                                     aGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCg                                                                          >  8:181640/1‑148 (MQ=255)
                                                                        tGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGAt                                                                                                                                   <  8:125053/88‑1 (MQ=255)
                                                                        tGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGAt                                                                                                                                   >  7:125053/1‑88 (MQ=255)
                                                                          cagcaTGTCCTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGt                                                                      <  8:256802/147‑1 (MQ=255)
                                                                            gcaTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGAtt                                                                  >  7:23886/1‑149 (MQ=255)
                                                                              aTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACg                                                                                                                   >  5:64987/1‑98 (MQ=255)
                                                                              aTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACg                                                                                                                   <  6:64987/98‑1 (MQ=255)
                                                                               tGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGt                                                               <  4:462390/149‑1 (MQ=255)
                                                                                 tCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACtt                                                                             <  1:565062/133‑1 (MQ=255)
                                                                                 tCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACtt                                                                             >  2:565062/1‑133 (MQ=255)
                                                                                 tCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTac                                                             <  6:317312/149‑1 (MQ=255)
                                                                                      cagcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAg                                                        >  2:585517/1‑149 (MQ=255)
                                                                                       agcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACGGACGCTGGAGAGGAAGTCTTCAACCAc                                                                               <  5:73623/125‑1 (MQ=255)
                                                                                       agcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGCCGCTCGAGAGGAAGTCTGCAACCAc                                                                               >  6:73623/1‑125 (MQ=255)
                                                                                        gcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCt                                                                                       >  3:247162/1‑116 (MQ=255)
                                                                                        gcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCt                                                                                       <  4:247162/116‑1 (MQ=255)
                                                                                         cagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTgg                                                                >  3:162458/1‑138 (MQ=255)
                                                                                         cagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTgg                                                                <  4:162458/138‑1 (MQ=255)
                                                                                          agGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCgg                                                     >  1:277709/1‑148 (MQ=255)
                                                                                            gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGc                                                       <  2:616083/144‑1 (MQ=255)
                                                                                            gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGc                                                       >  1:616083/1‑144 (MQ=255)
                                                                                            gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCt                                                                                       >  8:104100/1‑112 (MQ=255)
                                                                                            gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCt                                                                                       <  7:104100/112‑1 (MQ=255)
                                                                                            gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCa                                                                        >  8:56913/1‑127 (MQ=255)
                                                                                            gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCa                                                                        <  7:56913/127‑1 (MQ=255)
                                                                                            gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAAc                                                  <  3:166551/149‑1 (MQ=255)
                                                                                            gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAAc                                                  <  2:277709/149‑1 (MQ=255)
                                                                                             aTCCGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCATCGTGCAGATCCACTGTCAGCACACGGTCAACACCGAGGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACg                                                 <  5:69822/149‑1 (MQ=255)
                                                                                                gggCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGAc                                              <  7:181640/149‑1 (MQ=255)
                                                                                                 ggCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGAcg                                             >  3:417637/1‑149 (MQ=255)
                                                                                                       cAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACgg                                       >  3:32896/1‑149 (MQ=255)
                                                                                                              gTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCg                                                                          <  2:516401/107‑1 (MQ=255)
                                                                                                              gTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCg                                                                          >  1:516401/1‑107 (MQ=255)
                                                                                                                 aTCAACCGGAACGTCGAAGAAACCCTGAAAATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgcgc                              <  5:342879/148‑1 (MQ=255)
                                                                                                                  tCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgcgcc                             >  5:489019/1‑147 (MQ=255)
                                                                                                                    aaCCGGAACGTCGAAGAACCCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTCGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGACCGGAACGGACGCTACg                                        >  6:580474/1‑135 (MQ=255)
                                                                                                                    aaCCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACAGCGACGCTGGAGAGGAAGGCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACg                                        <  5:580474/135‑1 (MQ=255)
                                                                                                                                gaagaaACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAg               <  1:585517/148‑1 (MQ=255)
                                                                                                                                 aagaaACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGATCGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAggg             >  5:7338/1‑149 (MQ=255)
                                                                                                                                  agaaACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAgggg            <  6:489019/149‑1 (MQ=255)
                                                                                                                                   gaaACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGa           <  2:510446/149‑1 (MQ=255)
                                                                                                                                     aaCCCTGAATATGTTCAGCGTGCAGGTCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGAt          >  1:64698/1‑148 (MQ=255)
                                                                                                                                     aaCCCTGAATATGTTCAGCGTGCAGCTCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGAt          <  2:64698/148‑1 (MQ=255)
                                                                                                                                      aCCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATaa        >  4:172125/1‑149 (MQ=255)
                                                                                                                                       cccTGAATATGTTCAGCTTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAg                                                                       <  3:64768/85‑1 (MQ=255)
                                                                                                                                       cccTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAg                                                                       >  4:64768/1‑85 (MQ=255)
                                                                                                                                        ccTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgcgc                              >  6:381047/1‑125 (MQ=255)
                                                                                                                                        ccTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgcgc                              <  5:381047/125‑1 (MQ=255)
                                                                                                                                         cTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAg     >  6:320127/1‑149 (MQ=255)
                                                                                                                                            aaTCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCgg  <  7:458283/149‑1 (MQ=255)
                                                                                                                                            aaTATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCgg  >  6:157447/1‑149 (MQ=255)
                                                                                                                                            aaTATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCgg  <  1:350313/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
TTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCGG  >  NC_000913/1261331‑1261619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: