| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | 4226590 = | 48 (1.410) | 5 (0.150) | 5/256 | 3.5 | 9.7% | coding (2763/3684 nt) | metH | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| ? | NC_000913 | 4226621 = | 48 (1.480) | coding (2794/3684 nt) | metH | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent | |||||
| Rejected: Coverage evenness skew score above cutoff. | |||||||||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
TCAACGCCCACCACTTCATCTTCCAGAATGCGCGGATACTTCCCGGCCAGCGACCAGGTCATAAAGAACGGTGTCCAGTCGATGTAATTACGCAGCGTTTCGATGCTGGCTTCGACTTCCTGCACGCCGAGACGGTGCGCCACCGGCGGCGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < NC_000913/4226742‑4226590‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggcgtgCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAA > NC_000913/4226621‑4226761 tccACGCCCACCACTTCATCTTCCAGAATGCGCGGATACTTCCCGGCCAGCGACCAGGTCATAAAGAACGGTGTCCAGTCGATGTAATTACGCAGCGTTTCGATGCTGGCTTCGACTTCCTGCACGCCGAGACGGTGCGCCACCGGCGG > 8:102842/4‑149TCAACGCCCACCACTTCATCTTCCAGAATGCGCGGATACTTCCCGGCCAGCGACCAGGTCATAAAGAACGGTGTCCAGTCGATGTAATTACGCAGCGTTTCGATGCTGGCTTCGACTTCCTGCACGCCGAGACGGTGCGCCACCGGCGG > 8:101020/1‑149 AACGCCCACCACTTCATCTTCCAGAATGCGCGGATACTTCCCGGCCAGCGACCAGGTGATAAAGAACGGTGTCCAGTCGATGTAAGTACGCAGCGTTTCGATGCTGGCTTCGACTTCCTCCACGCCGAGAAGGTGCGCCACAGGCGGCG > 4:86100/1‑149 TTCCTGCACGCCGAGACGGTGCGCCACCGGCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAAGTATCCGCGCATTCTGGAAGATGAAGTGG > 5:80033/1‑149 TGCACGCCGAGACGGTGCGCCACCGGCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAA < 5:126062/116‑1 TGCACGCCGAGACGGTGCGCCACCGGCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAA > 6:126062/1‑116 ACGCCGAGACGGTGCGCCACCGGCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAAGTCTCCGCGCATTCTGGAAGATGAAGTGGTGGGCGT > 4:73102/1‑149 CGCCGAGACGGTGCGCCACCGGCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTT < 6:80033/149‑1 GGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAA < 2:65249/147‑1 TCAACGCCCACCACTTCATCTTCCAGAATGCGCGGATACTTCCCGGCCAGCGACCAGGTCATAAAGAACGGTGTCCAGTCGATGTAATTACGCAGCGTTTCGATGCTGGCTTCGACTTCCTGCACGCCGAGACGGTGCGCCACCGGCGGCGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < NC_000913/4226742‑4226590‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggcgtgCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGGCTGTTTAAA > NC_000913/4226621‑4226761 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |