| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,408,292 | 0 | T | A | 21.4% | 47.9 / 10.1 | 28 | R81R (CGT→CGA) | ydaN | putative Zn(II) transporter |
| Reads supporting (aligned to +/- strand): ref base T (12/10); new base A (2/4); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATG > NC_000913/1408156‑1408437 | atgaAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGACGGCTGACTCGGGGacgc < 7:123135/148‑4 (MQ=255) aTCCCTGTTGGCTCCAACTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGAtt < 1:140380/149‑1 (MQ=255) ttGGCTCCACCTTACTTATTTACACGTTGATAGCGCCGTTTGGCTGGGGACAAGACCGCTGGTTCCCATTATCGTACGTGATGCGCTGGCGGGCGAGAGGAGGCGTGCGCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACAtt < 5:87802/147‑1 (MQ=255) gCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCt > 2:26735/1‑149 (MQ=255) tCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGTGCTGGCGGGCGAGAGTACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTg > 6:63569/1‑148 (MQ=255) ttATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACgg > 1:23453/1‑147 (MQ=255) ggctggcGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGACGGCTGACTCggg < 8:24907/86‑2 (MQ=255) ggctggcGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGACGGCTGACTCggg > 7:24907/1‑85 (MQ=255) gACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGAt < 5:64809/124‑1 (MQ=255) gACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGAt > 6:64809/1‑124 (MQ=255) aacaCCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAATGCCCCGATCAACTGGTCGCCATGc < 7:72903/149‑1 (MQ=255) acCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACAtt < 2:141656/93‑1 (MQ=255) acCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACAtt > 1:141656/1‑93 (MQ=255) tgctTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGACCGCCCCGATCAACTGGTCGCCATGCGTGtata > 8:70420/1‑148 (MQ=255) tCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACTCGTCCCCGAGTCAGCCTTCTCGGATAAGGCACGCTGAATACATTGCGCTGTATAAACAGCAGCACCGa > 8:55657/1‑108 (MQ=255) tCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGGCAGCCGACTCGGTGAAGGCACGCTGATTTCATTGCGCTGTATAAACGGCAGCACCGa < 7:55657/108‑1 (MQ=255) cccAATAACGTACGTGATGTGCTGGCGGGCGAGAGTACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATg < 5:63569/147‑1 (MQ=255) tGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGttt < 1:26735/149‑1 (MQ=255) gcgTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGtt > 2:54898/1‑127 (MQ=255) gcgTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGtt < 1:54898/127‑1 (MQ=255) ttcaccGAGACGGCTGACTCGGGGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTg > 5:72974/5‑149 (MQ=255) gTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTg > 4:74667/1‑149 (MQ=255) gTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTg > 1:7977/1‑149 (MQ=255) caccGAGACGGCTGACTCGGGGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTg < 6:72974/145‑1 (MQ=255) ccGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGgcgc > 6:15189/1‑149 (MQ=255) ccGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGgcgc > 8:90979/1‑149 (MQ=255) gCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGAt < 2:23453/149‑1 (MQ=255) ccGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGAtg < 5:15189/149‑1 (MQ=255) | ATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATG > NC_000913/1408156‑1408437 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |