Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,294,680	Δ1 bp	5.6%	coding (225/1944 nt)	ccmF ←	heme lyase, CcmF subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,294,680	0	G	.	5.6%	237.0 / 7.1	72	coding (225/1944 nt)	ccmF	heme lyase, CcmF subunit
Reads supporting (aligned to +/- strand):  ref base G (35/32);  new base . (2/2);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01

CGGGCCACAATATCCAGCGGAATACGCTGACTAAAAATCGCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCC  >  NC_000913/2294534‑2294821                                                                                                                                                   |                                                                                                                                              cGGGCCACAATATCCAGCGGAATACGCTGACTAAAAATCGCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGa                                                                                                                                             <  3:205151/149‑1 (MQ=255)  gggCCACAATATCCAGCGGAATACGCTGACTAAAAATCGCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCCCGCGATACCACACCGGAAGCTGGGTATTGGAg                                                                                                                                            >  7:248542/1‑149 (MQ=255)         aaTATCCAGCGGAATACGCTGACTAAAAATCGCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTgg                                                                                                                                     >  8:341931/1‑149 (MQ=255)           tatCCAGCGGAATACGCTGACTAAAAATCGCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGc                                                                                                                                    >  8:98463/1‑148 (MQ=255)                aGCGGAATACGCTGACTAAAAATCGCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACa                                                                                                                                >  7:152821/1‑147 (MQ=255)                   ggAATACGCTGACTAAAAATCGCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGt                                                                                                                           >  5:240106/1‑149 (MQ=255)                        aCGCTGACTAAAAATCGCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATT‑GAGTTGCTGGCAACATAGGTGACGGt                                                                                                                     >  6:212925/1‑149 (MQ=255)                        aCGCTGACTAAAAATCGCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATT‑GAGTTGCTGGCAACATAGGTGACGGt                                                                                                                     >  6:207531/1‑149 (MQ=255)                          gCTGACTAAAAATAGCCCCCGCAAAGGTCCAGCCGCTCATCAGCAGCCCCCCCAGCAGTAGCGAGCCTTAATGCGCGCACCAGGTAGCCGCCCCGCGATACCACACCGGAAGCTGGGTATGGGAGTTGATTGCAACATAGGTGACgtt                                                                                                                     >  6:312560/1‑146 (MQ=255)                            tGACTAAAAATCGCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGACGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTg                                                                                                                    >  2:304933/1‑147 (MQ=255)                              aCTAAAAATCGCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAg                                                                                                                 <  6:240106/148‑1 (MQ=255)                              aCTAAAAATCGCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGt                                                                                                                >  1:66217/1‑149 (MQ=255)                              aCTAAAAATCGCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGt                                                                                                                >  2:255915/1‑149 (MQ=255)                              aCTAAAAATCGCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGt                                                                                                                >  3:314422/1‑149 (MQ=255)                                tAAAAATCGCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCg                                                                                                              >  7:46769/1‑149 (MQ=255)                                     aTCGCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGAc                                                                                                         <  8:152821/149‑1 (MQ=255)                                        gCCACCGCAAAGGTCCAGCCGCTCTTCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATAACACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCAc                                                                                                      >  1:278688/1‑149 (MQ=255)                                        gCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCAc                                                                                                      <  7:341931/149‑1 (MQ=255)                                         ccACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCaa                                                                                                                                  <  1:134676/120‑1 (MQ=255)                                         ccACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCaa                                                                                                                                  >  2:134676/1‑120 (MQ=255)                                         ccACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCAc                                                                                                      >  4:81415/1‑148 (MQ=255)                                          cACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGa                                                                                                    <  4:314422/149‑1 (MQ=255)                                              gCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAg                                                                                                 >  4:105745/1‑148 (MQ=255)                                              gCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGc                                                                                                <  8:327883/149‑1 (MQ=255)                                                  aGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTg                                                                                            >  5:282967/1‑149 (MQ=255)                                                    gTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGAc                                                                                          <  1:362971/149‑1 (MQ=255)                                                    gTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGAc                                                                                          <  8:1214/149‑1 (MQ=255)                                                    gTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGAc                                                                                          <  3:139388/149‑1 (MQ=255)                                                      ccAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCa                                                                                        <  2:186195/149‑1 (MQ=255)                                                         gCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCCCGCGATACCACACCGGAAGCGGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCCCGAAAGCATTGACCAGCa                                                                                     >  1:232480/1‑149 (MQ=255)                                                          ccGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCAc                                                                                    <  3:105745/149‑1 (MQ=255)                                                           cGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCAc                                                                                    >  3:328188/1‑148 (MQ=255)                                                           cGCTCATCAACAGCACCCACAGCAGTAGCGAGCCATCCTGCGCGACCCAGCCAGCCGCCACGCGATACAACACCGGCAGCGGGGAATAGGAGTTGCAGGCAACATAGGGGACGGTGAATTCGTTGACCACGAAACCATTGACCAGcccc                                                                                   <  1:318705/149‑1 (MQ=255)                                                             cTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAg                                                                                 <  8:144285/149‑1 (MQ=255)                                                                atcaGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATT‑GAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGaaa                                                                              <  5:207531/148‑1 (MQ=255)                                                                atcaGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATT‑GAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGaaa                                                                              <  5:212925/148‑1 (MQ=255)                                                                  cagcagCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTg                                                                                                           <  5:96811/118‑1 (MQ=255)                                                                  cagcagCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTg                                                                                                           >  6:96811/1‑118 (MQ=255)                                                                     cagcaCCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATgcgc                                                                         <  5:132895/149‑1 (MQ=255)                                                                       gcaCCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGACAGCATTGCCCAGCACCAGAAATgcgc                                                                         >  1:168325/1‑147 (MQ=255)                                                                       gcaCCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCgcca                                                                       <  1:255915/149‑1 (MQ=255)                                                                         aCCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCgccagc                                                                     >  3:175575/1‑149 (MQ=255)                                                                          cccACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGAcc                                                                                         >  4:218246/1‑128 (MQ=255)                                                                          cccACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGAcc                                                                                         <  3:218246/128‑1 (MQ=255)                                                                           ccACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCgccagcca                                                                   >  1:260832/1‑149 (MQ=255)                                                                             acaGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCgccagcca                                                                   >  8:56008/1‑147 (MQ=255)                                                                             acaGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCcaca                                                                 <  1:304933/149‑1 (MQ=255)                                                                             acaGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCcaca                                                                 <  3:81415/149‑1 (MQ=255)                                                                                 cagGAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGGCACGGGATACCACACGGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGGGACGGTGAAGTCGTTGAGCACGAAAGCATTGACCAGCTCCAGAAATGCGCCAGCCACAGACa                                                             <  4:348911/149‑1 (MQ=255)                                                                                  agTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACAt                                                            <  1:128208/149‑1 (MQ=255)                                                                                   gTAGCGAGCCTTCATGCGCGCCCCTGGTAGCCGCCACGCGATACGACCCCGGAAGCTGGGTTTTGGAGTTGCTGGCAACATAGGTGAGGGTGAAGTCGTTGAGGACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCCGAGACAt                                                            >  2:120991/1‑148 (MQ=255)                                                                                      gCGAGCCTTCATGCGCGGCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCATGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATaaa                                                         <  3:332015/148‑1 (MQ=255)                                                                                         aGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAAcag                                                      >  8:287570/1‑148 (MQ=255)                                                                                          gCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAAcagca                                                    <  8:248542/149‑1 (MQ=255)                                                                                             ttCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAACagcagc                                                  >  3:40854/1‑148 (MQ=255)                                                                                                aTGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAg                                               <  6:282967/148‑1 (MQ=255)                                                                                                aTGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGA‑CACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGc                                             <  2:66217/149‑1 (MQ=255)                                                                                                aTGCGCGCCCAAGGTAGCCGCCACGCGATACCACACAGGAAGATGGGTATTGGAGTTGCTGGCAACATAGTTGACGGTGAAGGCGTTGAAGACGAAAGCATTGATCATAACCAGAATTTCGCAAGCCACAGACATAAACATCAGCCAg                                               <  8:347519/148‑1 (MQ=255)                                                                                                     cgcCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTTGAGTTGATGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAAc                                         <  6:20621/149‑1 (MQ=255)                                                                                                         ccAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAg                                      >  8:240797/1‑148 (MQ=255)                                                                                                         ccAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAg                                      <  7:240797/148‑1 (MQ=255)                                                                                                          cAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTTAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAGCg                                    <  4:40854/149‑1 (MQ=255)                                                                                                          cAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAGCg                                    <  8:46769/149‑1 (MQ=255)                                                                                                              tAGCCGCCACGCGATACCACACCGGAAGCTGGGTAGTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAGCGGGaa                                <  3:286232/149‑1 (MQ=255)                                                                                                                          gATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATg                    >  7:315654/1‑149 (MQ=255)                                                                                                                               cacaCCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATgcgca                >  3:220857/1‑147 (MQ=255)                                                                                                                                  acCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGAc                                                                                          <  5:115309/71‑1 (MQ=255)                                                                                                                                  acCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGAc                                                                                          >  6:115309/1‑71 (MQ=255)                                                                                                                                    cGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCAtctc          >  3:184080/1‑149 (MQ=255)                                                                                                                                    cGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCAtctc          >  7:60207/1‑149 (MQ=255)                                                                                                                                        aGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCcgcg      >  1:49284/1‑149 (MQ=255)                                                                                                                                            gggTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGCCATAAACAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGcc  >  6:376942/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                              CGGGCCACAATATCCAGCGGAATACGCTGACTAAAAATCGCCACCGCAAAGGTCCAGCCGCTCATCAGCAGCACCCACAGCAGTAGCGAGCCTTCATGCGCGCCCCAGGTAGCCGCCACGCGATACCACACCGGAAGCTGGGTATTGGAGTTGCTGGCAACATAGGTGACGGTGAAGTCGTTGACCACGAAAGCATTGACCAGCACCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCC  >  NC_000913/2294534‑2294821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑