Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,263,541 T→C 5.5% intergenic (‑41/‑173) lolB ← / → hemA lipoprotein localization factor/glutamyl tRNA reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,263,5410TC 5.5% 230.0 / 3.3 73intergenic (‑41/‑173)lolB/hemAlipoprotein localization factor/glutamyl tRNA reductase
Reads supporting (aligned to +/- strand):  ref base T (35/34);  new base C (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01

ATCCGGGCTTTTGCCAGGACCTTTGGGCGTGGTAACGGAACAGGCAGTGAGCACAAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTAT  >  NC_000913/1263396‑1263687
                                                                                                                                                 |                                                                                                                                                  
aTCCGGGCTTTTGCCAGGACCTTTGGGCGTGGTAACGGAACAGGCAGTGAGCACAAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTaa                                                                                                                                                 <  4:128829/149‑1 (MQ=255)
        ttttGCCAGGACCTTTGGGCGTGGTAACGGAACAGGCAGTGAGCACAAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCg                                                                                                                                         >  7:61203/1‑149 (MQ=255)
                          gCGTGGTAACGGAACAGGCAGTGAGCACAAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTc                                                                                                                       >  5:128768/1‑149 (MQ=255)
                           cGTGGTAACGGAACAGGCAGTGAGCACAAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCt                                                                                                                      <  5:359672/149‑1 (MQ=255)
                             tGGTAACGGAACAGGCAGTGAGCACAAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTAt                                                                                                                    <  5:155927/149‑1 (MQ=255)
                                tAACGGAACAGGCAGTGAGCACAAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGGTATAACCCTTAATGCTAGCGTTACCGTCCGCTTTCGTCTTTGtt                                                                                                                 >  5:143046/1‑149 (MQ=255)
                                 aaCGGAACAGGCAGTGAGCACAAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGtt                                                                                                                 >  4:2709/1‑148 (MQ=255)
                                  aCGGAACAGGCAGTGAGCACAAGAGCAGCCAGCGGTAGCAGGCGTATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTc                                                                                                                <  3:24894/148‑1 (MQ=255)
                                    ggAACAGGCAGTGAGCACAAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGGCCGCTATCGTCTATGTTCAAg                                                                                                             >  1:249708/1‑149 (MQ=255)
                                     gAACAGGCAGTGAGCACAAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGt                                                                                                            >  3:167131/1‑149 (MQ=255)
                                      aaCAGGCAGTGAGCACAAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGtt                                                                                                           <  4:167131/149‑1 (MQ=255)
                                      aaCAGGCAGTGAGCACAACAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGtt                                                                                                           <  6:82447/149‑1 (MQ=255)
                                          ggCAGTGAGCACAAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCt                                                                                                       <  2:302621/149‑1 (MQ=255)
                                               tGAGCACAAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAAtt                                                                                                  <  1:396911/149‑1 (MQ=255)
                                               tGAGCACAAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAAtt                                                                                                  <  3:173226/149‑1 (MQ=255)
                                                 atcacaAGAGCAGCCAGGGGTAGCAGGCGGATATGACGAAAATCGGCCAGTGGCATAGTGATGACAAGTCCTTGAGATATGTTGCAGTTATAACCCTTAATGCTTGAGTTAACGTCCGCTATCGTCTATGTTCAATTTGTCTTAATTGc                                                                                                <  2:103448/147‑1 (MQ=255)
                                                 aGCACAAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGc                                                                                                >  6:163995/1‑149 (MQ=255)
                                                   cacaAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCa                                                                                              <  7:304568/149‑1 (MQ=255)
                                                   cacaAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCa                                                                                              <  7:309383/149‑1 (MQ=255)
                                                    acaAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGc                                                                                                >  1:95606/1‑146 (MQ=255)
                                                    acaAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAg                                                                                             <  3:357595/149‑1 (MQ=255)
                                                      aaGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGaa                                                                                           >  5:324345/1‑149 (MQ=255)
                                                      aaGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGaa                                                                                           >  3:213706/1‑149 (MQ=255)
                                                      aaGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGaa                                                                                           >  8:386128/1‑149 (MQ=255)
                                                        gagCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATc                                                                                         >  6:119671/1‑149 (MQ=255)
                                                        gagCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATc                                                                                         >  3:90444/1‑149 (MQ=255)
                                                           cagccagcGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCATCTATGTTCAAGTTGTCTTAATTGCCAGAATCTaa                                                                                      >  2:178412/1‑149 (MQ=255)
                                                                  cGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCttt                                                                               <  5:163995/149‑1 (MQ=255)
                                                                   ggTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTc                                                                              >  1:285575/1‑149 (MQ=255)
                                                                   ggTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTc                                                                              <  5:119671/149‑1 (MQ=255)
                                                                     tAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCgg                                                                            <  4:250801/149‑1 (MQ=255)
                                                                     tAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCgg                                                                            <  8:61203/149‑1 (MQ=255)
                                                                                   gACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCaa                                                                >  6:368494/1‑147 (MQ=255)
                                                                                   gACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCaa                                                                >  1:360328/1‑147 (MQ=255)
                                                                                   gACGAAAATCGGGCAGGGGCATAGTGATGAAAAGTACTTTAGATACGTTGCAGTTATAACCCTTAATGCTAGAGTTACAGTCCGCTATCGTCTATGTTCAAGTGGTCTTAATTGCCAGAATCTAAAGGCTTTAGGCAATTaagcgca                                                                >  6:376389/1‑141 (MQ=255)
                                                                                     cGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAgg                                                            >  8:392764/1‑149 (MQ=255)
                                                                                     cGAAAATCGGGCAGAGGCATAGTGATGACAAGTCCTTGTGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAgg                                                            <  8:163508/149‑1 (MQ=255)
                                                                                      gAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAgg                                                            <  6:23348/148‑1 (MQ=255)
                                                                                      gAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAgg                                                            >  5:23348/1‑148 (MQ=255)
                                                                                             gggCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGctc                                                    >  8:52994/1‑149 (MQ=255)
                                                                                             gggCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGctc                                                    >  8:59300/1‑149 (MQ=255)
                                                                                                cAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCCTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAAt                                                                        >  7:205863/1‑126 (MQ=255)
                                                                                                cAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCCTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAAt                                                                        <  6:1320/126‑1 (MQ=255)
                                                                                                cAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCCTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAAt                                                                        <  8:205863/126‑1 (MQ=255)
                                                                                                cAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCCTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAAt                                                                        >  5:1320/1‑126 (MQ=255)
                                                                                                     gCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTAt                                            <  5:368494/149‑1 (MQ=255)
                                                                                                      cATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTAtt                                           <  1:299476/149‑1 (MQ=255)
                                                                                                      cATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTAAGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCCTCAATTACTCCAAAATGGGTCGCTCTCTTTTAtt                                           >  5:108901/1‑149 (MQ=255)
                                                                                                       aTAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACCGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTAtt                                           >  6:14190/1‑148 (MQ=255)
                                                                                                          gTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGAt                                        <  2:249708/148‑1 (MQ=255)
                                                                                                             atgaCAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTc                                                                                                                >  4:253304/1‑73 (MQ=255)
                                                                                                             atgaCAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTc                                                                                                                <  3:253304/73‑1 (MQ=255)
                                                                                                              tgaCAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTAc                                   <  3:2709/149‑1 (MQ=255)
                                                                                                               gaCAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGtt                                                                                                                 >  8:901/1‑70 (MQ=255)
                                                                                                                   aGTCCTTGAGATACGGTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATc                              <  8:120056/149‑1 (MQ=255)
                                                                                                                    gTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATcc                             >  2:179695/1‑149 (MQ=255)
                                                                                                                     tCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCt                            <  6:324345/149‑1 (MQ=255)
                                                                                                                       cTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTTTGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTAGTGATCTTACGCATCCTg                           >  6:348902/1‑148 (MQ=255)
                                                                                                                       cTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTg                           <  2:285575/148‑1 (MQ=255)
                                                                                                                       cTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGt                          <  7:386128/149‑1 (MQ=255)
                                                                                                                        ttGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTa                                    <  5:358700/138‑1 (MQ=255)
                                                                                                                        ttGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTa                                    >  6:358700/1‑138 (MQ=255)
                                                                                                                           agaTACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTatga                      <  4:213706/149‑1 (MQ=255)
                                                                                                                            gaTACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTatgat                     >  7:374619/1‑149 (MQ=255)
                                                                                                                                   tGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGaa                                                                                           >  3:219188/1‑72 (MQ=255)
                                                                                                                                   tGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGaa                                                                                           <  4:219188/72‑1 (MQ=255)
                                                                                                                                      aGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGAc           <  2:360328/149‑1 (MQ=255)
                                                                                                                                       gTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTat                        <  3:124416/135‑1 (MQ=255)
                                                                                                                                       gTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTat                        >  4:124416/1‑135 (MQ=255)
                                                                                                                                        ttATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAg                >  7:327349/1‑142 (MQ=255)
                                                                                                                                        ttATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAg                <  8:327349/142‑1 (MQ=255)
                                                                                                                                               ccTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTAt  >  2:3938/1‑149 (MQ=255)
                                                                                                                                               ccTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTAt  >  4:225280/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ATCCGGGCTTTTGCCAGGACCTTTGGGCGTGGTAACGGAACAGGCAGTGAGCACAAGAGCAGCCAGCGGTAGCAGGCGGATAAGACGAAAATCGGGCAGGGGCATAGTGATGACAAGTCCTTGAGATACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTAT  >  NC_000913/1263396‑1263687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: