New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 284721730 (0.970)4 (0.140) 4/256 3.3 13.1% coding (196/924 nt) hycD hydrogenase 3, membrane subunit
?NC_000913 = 2847253 25 (0.860)coding (160/924 nt) hycD hydrogenase 3, membrane subunit
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGTTATACCGGAGAGCAGCGGCGCAACGGCAAATAACACCAGCGCCTGAATTAACGGA  >  NC_000913/2847112‑2847398
                                                                                                                                             |                                                                                                                                                 
gTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGc                                                                                                                                            <  7:108299/149‑1 (MQ=255)
       gATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCcag                                                                                                                                       >  2:48196/1‑147 (MQ=255)
        aTCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCcagcag                                                                                                                                    >  3:120713/1‑149 (MQ=255)
           aaaTCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGttt                                                                                                                                 <  1:58916/149‑1 (MQ=255)
           aaaTCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGttt                                                                                                                                 <  3:109835/149‑1 (MQ=255)
           aaaTCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGGCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGttt                                                                                                                                 <  8:71601/149‑1 (MQ=255)
            aaTCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGttt                                                                                                                                 >  6:41750/1‑148 (MQ=255)
             aTCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGTCCCAGCAGTTTGa                                                                                                                               <  5:7474/149‑1 (MQ=255)
              tCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCACAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGAt                                                                                                                              <  6:65285/149‑1 (MQ=255)
                aCCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATaa                                                                                                                            <  6:45438/149‑1 (MQ=255)
                aCCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATaa                                                                                                                            <  4:120713/149‑1 (MQ=255)
                           gCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATAc                                                                                                                 <  5:41750/149‑1 (MQ=255)
                                    aaCCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAac                                                                                                        <  1:48196/149‑1 (MQ=255)
                                                ccaccGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACgcccggcccg                                                                                            >  7:82076/1‑149 (MQ=255)
                                                      gCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCGGCAACACGCCCGGCCCGCGACgg                                                                                      >  6:36851/1‑149 (MQ=255)
                                                                  cGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCGGCAACACGCCCGGCCCGCGACGGTTATGCAAGCgg                                                                          <  5:36851/149‑1 (MQ=255)
                                                                   gATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCggg                                                                         >  7:122311/1‑149 (MQ=255)
                                                                   gATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCggg                                                                         <  8:82076/149‑1 (MQ=255)
                                                                                  gCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTGTGATAATGTCGCGCTACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCggg                                                          <  7:119677/149‑1 (MQ=255)
                                                                                   cccACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCa                                                                <  4:15216/142‑1 (MQ=255)
                                                                                   cccACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCa                                                                >  3:15216/1‑142 (MQ=255)
                                                                                                          gCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGTTATACCGGAGAGCAGCGGCGCaa                                  >  1:120810/1‑149 (MQ=255)
                                                                                                              aaCACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGTTATACCGGAGAGCAGCGGCGCAACgg                               >  4:26984/1‑148 (MQ=255)
                                                                                                                       ccGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGTTATACCGGAGAGCAGCGGCGCAACGGCAAATAacac                     <  3:26984/149‑1 (MQ=255)
                                                                                                                       ccGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGTTATACCGGAGAGCAGCGGCGCAACGGCAAATAacac                     <  8:122311/149‑1 (MQ=255)
                                                                                                                                          cGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGTTATACCGGAGAGCAGCGGCGCAACGGCAAATAACACCAGCGCCTGAATTAACGGa  <  2:120810/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
GTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGTTATACCGGAGAGCAGCGGCGCAACGGCAAATAACACCAGCGCCTGAATTAACGGA  >  NC_000913/2847112‑2847398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.