New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2557337 =	64 (0.710)	4 (0.050)	4/266	9.2	6.2%	coding (1343/1362 nt)	eutB	ethanolamine ammonia‑lyase, large subunit, heavy chain
	?	NC_000913	2557361 =	59 (0.680)					coding (1319/1362 nt)	eutB	ethanolamine ammonia‑lyase, large subunit, heavy chain
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTTTGCTTCTGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTAGTTGAGCATGATGTCATCACCCAGCGGCATCCCCAT  >  NC_000913/2557214‑2557506                                                                                                                                                    |                                                                                                                                                  cTTTGCTTCTGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGAcc                                                                                                                                                  <  7:16645/149‑1 (MQ=255)    tGCTTCTGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGtt                                                                                                                                               >  6:8646/1‑149 (MQ=255)      cTTCTGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGttt                                                                                                                                              <  7:244647/148‑1 (MQ=255)      cTTCTGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTg                                                                                                                                             <  8:226206/149‑1 (MQ=255)       ttCTGACGGCGCGGGGGCCGCTTGTCCTATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGc                                                                                                                                            >  7:67924/1‑149 (MQ=255)          tGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCa                                                                                                                                          >  4:83201/1‑148 (MQ=255)            aCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTTACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATaa                                                                                                                                       <  3:109691/149‑1 (MQ=255)              ggCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGAACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATaacg                                                                                                                                     >  2:385076/1‑147 (MQ=255)                   gggggCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCAt                                                                                                                                <  3:266029/149‑1 (MQ=255)                         cGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTc                                                                                                                          >  8:35540/1‑149 (MQ=255)                          gCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTc                                                                                                                          >  2:99632/1‑148 (MQ=255)                           cTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTcca                                                                                                                        <  7:35540/149‑1 (MQ=255)                                 ccATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCcagccagc                                                                                                                  >  8:14118/1‑149 (MQ=255)                                   aTTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATCATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGATTGCCATAATGCCCATGCTTTCCAGCCAGCGt                                                                                                                >  1:130533/1‑149 (MQ=255)                                      gACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTc                                                                                                              <  3:165077/148‑1 (MQ=255)                                       aCGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCaa                                                                                                            <  3:371201/149‑1 (MQ=255)                                           cATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTc                                                                                                        >  8:312124/1‑149 (MQ=255)                                            aTCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTcc                                                                                                       <  6:16064/149‑1 (MQ=255)                                             tCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATaa                                                                                                                                       <  6:105137/116‑1 (MQ=255)                                             tCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATaa                                                                                                                                       >  5:105137/1‑116 (MQ=255)                                             tCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTcca                                                                                                                        >  2:243855/1‑131 (MQ=255)                                             tCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCg                                                                                                      >  5:4257/1‑149 (MQ=255)                                                   tGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGAc                                                                                                                                                   <  8:181180/98‑1 (MQ=255)                                                   tGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGAc                                                                                                                                                   >  7:181180/1‑98 (MQ=255)                                                   tGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACg                                                                                                >  6:124522/1‑149 (MQ=255)                                                        aCAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGgcg                                                                                            >  3:372949/1‑148 (MQ=255)                                                                  cAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCATGTTGAg                                                                                   <  8:67924/147‑1 (MQ=255)                                                                  cAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGa                                                                                    <  8:76455/146‑1 (MQ=255)                                                                   aaTCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTaa                                                                                <  3:83201/149‑1 (MQ=255)                                                                   aaTCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTaa                                                                                <  6:222442/149‑1 (MQ=255)                                                                    aTCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAAc                                                                               <  7:14118/149‑1 (MQ=255)                                                                         tttttGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACt                                                                              <  4:372949/145‑1 (MQ=255)                                                                         tttttGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATAGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGAGGGGCGCAGGTTGAGTAACTGACg                                                                          >  7:189982/1‑149 (MQ=255)                                                                               aTCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCAAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGt                                                                    >  8:195621/1‑149 (MQ=255)                                                                                   aTGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAACGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCg                                                                <  1:385076/149‑1 (MQ=255)                                                                                           ttATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATTCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTg                                                        >  3:199743/1‑149 (MQ=255)                                                                                           ttATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTg                                                        >  4:130573/1‑149 (MQ=255)                                                                                           ttATCTCCGCGTCATCAGAAGAACAGTGACGGAGCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGt                                                         <  2:132235/148‑1 (MQ=255)                                                                                              tctcCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGa                                                      <  1:99632/148‑1 (MQ=255)                                                                                               ctcCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCg                                                                                                      <  4:371853/99‑1 (MQ=255)                                                                                               ctcCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCg                                                                                                      >  3:371853/1‑99 (MQ=255)                                                                                                        atcaGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTc                                           <  8:217312/149‑1 (MQ=255)                                                                                                           agaagaACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTgg                                        >  6:185392/1‑149 (MQ=255)                                                                                                           agaagaACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTgg                                        >  7:37295/1‑149 (MQ=255)                                                                                                            gaagaaCAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTgg                                        >  3:122530/1‑148 (MQ=255)                                                                                                             aagaaCAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTgg                                        >  4:88321/1‑147 (MQ=255)                                                                                                                aaCAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTAGtt                                   <  6:4257/149‑1 (MQ=255)                                                                                                                    gTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGATTGCGGTGGTCTGGTAGTTgcg                                >  6:93408/1‑146 (MQ=255)                                                                                                                       aCGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCATCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTAGTTGAGCatg                            <  5:8646/149‑1 (MQ=255)                                                                                                                           aTCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACgg                                                                                               <  2:133053/78‑1 (MQ=255)                                                                                                                           aTCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACgg                                                                                               >  1:133053/1‑78 (MQ=255)                                                                                                                               cccgcccgTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTAGTTGAGCATGATGtc                       >  6:1577/1‑146 (MQ=255)                                                                                                                               cccgcccgTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTAGTTGAGCATGATGtc                       <  5:1577/146‑1 (MQ=255)                                                                                                                                ccgcccgTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTAGTTGAGCATGATGtcatca                   >  1:10839/1‑149 (MQ=255)                                                                                                                                      gTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTAGtt                                   <  8:59958/127‑1 (MQ=255)                                                                                                                                      gTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTAGTTGAGCATGATGTCATCACCCAGc             >  3:276459/1‑149 (MQ=255)                                                                                                                                           gTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACg                                                                          >  6:78593/1‑83 (MQ=255)                                                                                                                                           gTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACg                                                                          <  5:78593/83‑1 (MQ=255)                                                                                                                                           ctCAGGCTACCGTTTGCCATAATGCCCATGCTATCAAACAAGCGTTCAAAATCAGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGAATCGTGGAATGCGGTGGTCTGGTAGTTGAGCATGATGTCATCACCCAGCGGCAt        >  2:293092/2‑149 (MQ=255)                                                                                                                                                  gACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTAGTTGAGCATGATGTCATCACCCAGCGGCATCCCCat  >  4:158924/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CTTTGCTTCTGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCGACCGTTTGCCATAATGCCCATGCTTTCCAGCCAGCGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTAGTTGAGCATGATGTCATCACCCAGCGGCATCCCCAT  >  NC_000913/2557214‑2557506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.