Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	759,249	C→T	R182C (CGC→TGC)	sucA →	2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	759,249	0	C	T	100.0%	271.3 / NA	77	R182C (CGC→TGC)	sucA	2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/38);  total (39/38)

CAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTG  >  NC_000913/759103‑759392                                                                                                                                                   |                                                                                                                                                cAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGc                                                                                                                                               >  2:247560/1‑149 (MQ=255)  aGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATACGCACATTACCAGCACCGAAGAAAAATGCt                                                                                                                                              <  8:312913/149‑1 (MQ=255)   gACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCt                                                                                                                                              >  4:185375/1‑148 (MQ=255)   gACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCt                                                                                                                                              <  3:185375/148‑1 (MQ=255)     cTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATCCTGGa                                                                                                                                           <  4:247216/149‑1 (MQ=255)     attCCAGTAGACCTTCAACGTCGGATCAATTGCCAGCGGCAAAGAAACCTTTAATCTCGTCGATCTTCGGTAAACCCTCAAGCAAACCTACTGCGTCCCGATTTGTGCCGAGTATATGCACATTACCAGCACCGAACAAAAATGCTGGa                                                                                                                                           <  5:193543/148‑1 (MQ=255)        ccAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATc                                                                                                                                         <  8:407887/148‑1 (MQ=255)         cAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATcc                                                                                                                                        <  5:183078/148‑1 (MQ=255)              gaCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGc                                                                                                                                  <  8:164672/149‑1 (MQ=255)                    cAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATc                                                                                                                             <  2:346765/148‑1 (MQ=255)                     aaCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATc                                                                                                                             >  7:85739/1‑147 (MQ=255)                      aCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAg                                                                                                                          <  3:363283/149‑1 (MQ=255)                        gTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTc                                                                                                                        >  6:209955/1‑149 (MQ=255)                         tCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCt                                                                                                                       <  1:43587/149‑1 (MQ=255)                           ggTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCGGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTg                                                                                                                      <  8:159024/148‑1 (MQ=255)                             ttCATTTGCCAGCTGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTc                                                                                                                   <  2:196239/149‑1 (MQ=255)                                aTTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTcgcg                                                                                                                >  7:305505/1‑149 (MQ=255)                                 tttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTcgcgc                                                                                                               >  3:169835/1‑149 (MQ=255)                                 tttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTcgcgc                                                                                                               >  1:210700/1‑149 (MQ=255)                                    gCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGa                                                                                                             <  4:169835/148‑1 (MQ=255)                                     ccAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGt                                                                                                                                >  1:189820/1‑128 (MQ=255)                                     ccAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGt                                                                                                                                <  2:189820/128‑1 (MQ=255)                                      cAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACtt                                                                                                          <  8:223994/149‑1 (MQ=255)                                       aGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACttt                                                                                                         >  3:291154/1‑149 (MQ=255)                                          ggCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCaa                                                                                                      <  8:305505/149‑1 (MQ=255)                                           gCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGTACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCaa                                                                                                      >  7:380256/1‑148 (MQ=255)                                             aaaGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAg                                                                                                   >  1:251106/1‑149 (MQ=255)                                              aaGAAACCATGAAACTCGGCGAGCTGCCGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGc                                                                                                  >  6:72057/1‑149 (MQ=255)                                                gAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCg                                                                                                 >  7:80538/1‑148 (MQ=255)                                                 aaaCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCTAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCg                                                                                                 >  3:284077/1‑147 (MQ=255)                                                  aaCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCg                                                                                                 >  7:383324/1‑146 (MQ=255)                                                   aCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGTACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAaga                                                                                             <  8:380256/149‑1 (MQ=255)                                                     cATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGc                                                                                                  <  6:296451/142‑1 (MQ=255)                                                     cATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGc                                                                                                  >  5:296451/1‑142 (MQ=255)                                                      aTGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaa                                                                                          <  4:74790/149‑1 (MQ=255)                                                       tGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaa                                                                                         <  2:210700/149‑1 (MQ=255)                                                       tGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaa                                                                                         <  8:383324/149‑1 (MQ=255)                                                             tCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATc                                                                                                                             >  8:95986/1‑107 (MQ=255)                                                               ggCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGc                                                                                   <  2:281254/147‑1 (MQ=255)                                                                gCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATACTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGcttc                                                                                <  7:123413/149‑1 (MQ=255)                                                                     ctgctcGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAg                                                                           <  1:159276/149‑1 (MQ=255)                                                                        ccggAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGc                                                                          <  5:72057/145‑1 (MQ=255)                                                                         tgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGaa                                                                       >  7:269889/1‑149 (MQ=255)                                                                                  tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAccgcc                                                              >  3:130517/1‑149 (MQ=255)                                                                                  tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAccgcc                                                              >  3:307399/1‑149 (MQ=255)                                                                                  tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACAAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAcaaat                                                              >  7:190791/1‑145 (MQ=255)                                                                                      gCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaaaaa                                                                                      <  3:249660/121‑1 (MQ=255)                                                                                      gCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaaaaa                                                                                      >  4:249660/1‑121 (MQ=255)                                                                                      gCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCt                                                           >  4:60924/1‑148 (MQ=255)                                                                                        aaaCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTg                                                          >  1:204963/1‑147 (MQ=255)                                                                                         aaCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGGCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg                                                       <  5:285744/149‑1 (MQ=255)                                                                                          aCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAgg                                                      >  8:273593/1‑149 (MQ=255)                                                                                                tGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGGATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTg                                                 >  5:214321/1‑148 (MQ=255)                                                                                                  cGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCTAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAAc                                              <  4:284077/149‑1 (MQ=255)                                                                                                  cGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGaa                                               <  1:29252/148‑1 (MQ=255)                                                                                                   ggCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCtt                                                  >  7:211851/1‑144 (MQ=255)                                                                                                   ggCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCtt                                                  <  8:211851/144‑1 (MQ=255)                                                                                                         aTTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAAc                                              <  2:137215/142‑1 (MQ=255)                                                                                                         aTTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAAc                                              >  1:137215/1‑142 (MQ=255)                                                                                                               gCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgcg                                  >  3:367878/1‑148 (MQ=255)                                                                                                                          gCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTg                      >  2:369688/1‑149 (MQ=255)                                                                                                                            acaTTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgc                    >  4:161750/1‑149 (MQ=255)                                                                                                                              aTTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcg                   >  6:360109/1‑148 (MQ=255)                                                                                                                               ttACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAATCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcgc                  >  6:309661/1‑148 (MQ=255)                                                                                                                               ttACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcgc                  >  6:162485/1‑148 (MQ=255)                                                                                                                                  ccAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCaaaa              >  3:205669/1‑149 (MQ=255)                                                                                                                                  ccAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCaaaa              >  8:81706/1‑149 (MQ=255)                                                                                                                                  ccAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCaaaa              <  7:273593/149‑1 (MQ=255)                                                                                                                                  ccAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCaaa               <  4:130517/148‑1 (MQ=255)                                                                                                                                  ccAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCaaa               <  1:247560/148‑1 (MQ=255)                                                                                                                                    aGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACg            >  2:395935/1‑149 (MQ=255)                                                                                                                                         cGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTccc                        <  8:167736/132‑1 (MQ=255)                                                                                                                                         cGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTccc                        >  7:167736/1‑132 (MQ=255)                                                                                                                                            agaaAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGc    <  4:307399/149‑1 (MQ=255)                                                                                                                                             gaaAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCt   >  3:60656/1‑149 (MQ=255)                                                                                                                                              aaaaaTGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTg  <  2:251106/149‑1 (MQ=255)                                                                                                                                              aaaaaTGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTg  <  4:291154/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                CAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTG  >  NC_000913/759103‑759392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑