Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,317,667	T→G	E737A (GAA→GCA)	rcsC ←	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,317,667	0	T	G	100.0%	187.6 / NA	52	E737A (GAA→GCA)	rcsC	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/27);  total (25/27)

CCACACTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAA  >  NC_000913/2317521‑2317809                                                                                                                                                   |                                                                                                                                               ccACACTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCg                                                                                                                                              <  1:307201/149‑1 (MQ=255)   acacTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTa                                                                                                                                            >  6:376983/1‑149 (MQ=255)     acTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTAtg                                                                                                                                          >  6:320456/1‑149 (MQ=255)     acTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTAtg                                                                                                                                          <  3:365230/149‑1 (MQ=255)                aCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAacgac                                                                                                                                >  5:282550/1‑148 (MQ=255)                  tCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTa                                                                                                                                            <  1:295568/134‑1 (MQ=255)                  tCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTa                                                                                                                                            >  2:295568/1‑134 (MQ=255)                      cTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcg                                                                                                                         >  8:8702/1‑149 (MQ=255)                          cgcTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTCCCTCGTCAGTGATCAACCCATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcgctgc                                                                                                                     >  1:272451/1‑149 (MQ=255)                            cTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCGTCGGGAGTCGGTTCCTGCCCTGCGTATGTGGTTACGACGATGCcgctgcgc                                                                                                                   >  8:385956/1‑149 (MQ=255)                             tttCTCCAGAGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcgctgcgct                                                                                                                  >  2:254884/1‑149 (MQ=255)                              ttCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcgctgcgctg                                                                                                                 >  7:1833/1‑149 (MQ=255)                                 tcCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGt                                                                                                                                             <  2:288427/118‑1 (MQ=255)                                           tACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTcc                                                                                                    <  5:193822/149‑1 (MQ=255)                                             ccAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCa                                                                                                   <  6:282550/148‑1 (MQ=255)                                             ccAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCa                                                                                                   <  5:376983/148‑1 (MQ=255)                                             ccAATATGGCGACGACAGAAGATCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAg                                                                                                  >  6:262258/1‑149 (MQ=255)                                               aaTATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcgctgcgc                                                                                                                   <  6:76745/130‑1 (MQ=255)                                                   tGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTg                                                                                            <  8:1833/149‑1 (MQ=255)                                                    ggCGACGACAGAAGGTCACTACCGCTCTGCCCTGCAATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGTCCCTGCGCTGCAAACTGGTTTCCAGGAACTGa                                                                                           >  2:385532/1‑149 (MQ=255)                                                     gCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGAt                                                                                                                             >  3:167113/1‑114 (MQ=255)                                                     gCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGAt                                                                                                                             <  4:167113/114‑1 (MQ=255)                                                            cAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGa                                                                                   <  8:237861/149‑1 (MQ=255)                                                                     aCTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGc                                                                           <  4:76402/148‑1 (MQ=255)                                                                       tACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGa                                                                                           <  6:52155/130‑1 (MQ=255)                                                                       tACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGa                                                                                           >  5:52155/1‑130 (MQ=255)                                                                       tACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGa                                                                        >  2:204420/1‑149 (MQ=255)                                                                         ccGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGAc                                                                       <  7:8702/148‑1 (MQ=255)                                                                           gCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGGCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCgc                                                                    <  4:298015/149‑1 (MQ=255)                                                                            ctctGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCgcc                                                                   <  2:50133/149‑1 (MQ=255)                                                                            ctctGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCgc                                                                    >  5:40345/1‑148 (MQ=255)                                                                             tctGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGGTTCCAGGAACTGACAGAGCGACGCATTGCGGACCgc                                                                    >  6:373613/1‑147 (MQ=255)                                                                                  cctgccATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCTGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGAc                                                                                  <  4:238394/128‑1 (MQ=255)                                                                                  cctgccATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCAGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGAc                                                                                  >  3:238394/1‑128 (MQ=255)                                                                                         ttttttACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGtt                                                      >  2:317680/1‑149 (MQ=255)                                                                                                       ccTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCt                                        <  1:317680/149‑1 (MQ=255)                                                                                                        cTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGTCGGTGCGCTGCAAACTGGTTTCAAGGAACTGACATAGCTACGCATTGCGGACCTCCTGCCAGCAGCGTTTACCACTCAACCCtt                                       <  1:385532/149‑1 (MQ=255)                                                                                                        cTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCtt                                       >  1:254105/1‑149 (MQ=255)                                                                                                                   tCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCtttt                            <  5:262258/149‑1 (MQ=255)                                                                                                                   tCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCtttt                            >  5:116006/1‑149 (MQ=255)                                                                                                                     aaCACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCtttttt                          >  7:322195/1‑149 (MQ=255)                                                                                                                      acacATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCATTCCACGCCTTTTTTc                         >  1:82374/1‑149 (MQ=255)                                                                                                                          aTCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTa                                                    <  1:316361/118‑1 (MQ=255)                                                                                                                          aTCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTa                                                    >  2:316361/1‑118 (MQ=255)                                                                                                                               cGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCgg                    <  6:116006/145‑1 (MQ=255)                                                                                                                               cGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCgg                    <  5:320456/145‑1 (MQ=255)                                                                                                                               cGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCgg                    <  8:310304/145‑1 (MQ=255)                                                                                                                                 ggAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTg              <  8:273995/149‑1 (MQ=255)                                                                                                                                  gAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTACAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGa             <  3:145689/149‑1 (MQ=255)                                                                                                                                       ggTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGcc        >  6:204809/1‑149 (MQ=255)                                                                                                                                         ttCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTTCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGt      <  2:272451/149‑1 (MQ=255)                                                                                                                                             tGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACaa  >  8:162249/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                               CCACACTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAA  >  NC_000913/2317521‑2317809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑