Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,440,924 C→A G36C (GGC→TGC)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,440,9240CA98.0% 346.1 / NA 100G36C (GGC→TGC) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (46/52);  minor base T (0/2);  total (46/54)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

CAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAG  >  NC_000913/3440793‑3441069
                                                                                                                                   |                                                                                                                                                 
cAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTaaa                                                                                                                                   <  5:80292/148‑1 (MQ=255)
       tCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAg                                                                                                                           >  5:376303/1‑149 (MQ=255)
         ttCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTATAAAGGCTCAAggg                                                                                                                         >  3:94977/1‑149 (MQ=255)
            cTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTc                                                                                                                       <  7:155578/148‑1 (MQ=255)
            cTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTc                                                                                                                       <  1:5592/148‑1 (MQ=255)
            cTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTc                                                                                                                       <  7:67998/148‑1 (MQ=255)
             tGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCAc                                                                                                                     >  1:196186/1‑149 (MQ=255)
             tGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCAc                                                                                                                     >  7:378636/1‑149 (MQ=255)
               aaCGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCAcc                                                                                                                    <  2:196186/148‑1 (MQ=255)
               aaCGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCt                                                                                                                   <  4:246531/149‑1 (MQ=255)
               aaCGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTATAAAGGCTCAAGGGTCAc                                                                                                                     <  4:94977/147‑1 (MQ=255)
                aCGCCTTCTTTGTTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCt                                                                                                                   <  6:186138/148‑1 (MQ=255)
                   ccTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgc                                                                                                               >  1:150469/1‑149 (MQ=255)
                    cttcttTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAgg                                                                                                                          <  4:315885/137‑1 (MQ=255)
                        tttGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtgcg                                                                                                          >  5:62257/1‑149 (MQ=255)
                          tGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtgc                                                                                                           <  3:284948/146‑1 (MQ=255)
                             tggtGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTaa                                                                                                                                    <  5:272524/115‑1 (MQ=255)
                             tGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTaa                                                                                                                                    >  6:272524/1‑118 (MQ=255)
                               cTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACt                                                                                                   >  6:181436/1‑149 (MQ=255)
                               cTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACt                                                                                                   <  5:181436/149‑1 (MQ=255)
                                tGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACt                                                                                                   >  5:116491/1‑148 (MQ=255)
                                  tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCAc                                                                                                >  3:147519/1‑149 (MQ=255)
                                    cTCATGTAGTACACCATCAATCTCAACCTCGGTCACGGCGGTACCCGGCATCGTTGAGAGCAGAATACGGCTCAGTGCGTTACCCAGAGTATGGCAAATGGCACTCTCTAATGTGTATAGGTTGACCTTGGCGTTCGTCGATCTCACt                                                                                               <  8:189969/148‑1 (MQ=255)
                                     tCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTg                                                                                             >  5:172909/1‑149 (MQ=255)
                                       aTGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                           >  5:229559/1‑149 (MQ=255)
                                        tGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                          <  2:334304/149‑1 (MQ=255)
                                        tGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                          >  7:329410/1‑149 (MQ=255)
                                        tGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                          <  7:359061/149‑1 (MQ=255)
                                        tGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGGCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                          <  5:375941/149‑1 (MQ=255)
                                         gtagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAACGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                          >  4:208958/1‑148 (MQ=255)
                                          tagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAAc                                                                                                    <  7:403435/137‑1 (MQ=255)
                                          tagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAAc                                                                                                    >  8:403435/1‑137 (MQ=255)
                                             taCACCATCAATCTCAACCTCGGTCACCGCGCCACCCGGCATCGATGATAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAACAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGACCTCACTTGCTc                                                                                          >  1:114711/1‑144 (MQ=255)
                                               cacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATc                                                                                    >  1:273894/1‑148 (MQ=255)
                                               cacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATc                                                                                    <  6:21918/148‑1 (MQ=255)
                                               cacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCAGCGATGAGCGCAGAATACGGCGCAGGGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGGCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATc                                                                                    <  1:372741/148‑1 (MQ=255)
                                                acCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCaaaa                                                                                                                                                <  7:263342/87‑1 (MQ=255)
                                                acCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCaaaa                                                                                                                                                >  8:263342/1‑87 (MQ=255)
                                                 ccATCAATCCCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgt                                                                                                             >  3:293813/1‑121 (MQ=255)
                                                 ccATCAATCCCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgt                                                                                                             <  4:293813/121‑1 (MQ=255)
                                                  cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCCCCTTGGCGTGCGTCGAACTCACTTGCTCGATATCaa                                                                                  >  5:269906/1‑147 (MQ=255)
                                                  cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCaa                                                                                  <  6:269906/147‑1 (MQ=255)
                                                   aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCaa                                                                                                                            >  4:242738/1‑104 (MQ=255)
                                                   aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCaa                                                                                                                            <  3:242738/104‑1 (MQ=255)
                                                    tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtgcgt                                                                                                         <  7:76925/122‑1 (MQ=255)
                                                    tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtgcgt                                                                                                         >  8:76925/1‑122 (MQ=255)
                                                    tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGGTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAg                                                                              <  6:385396/149‑1 (MQ=255)
                                                     cAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAgg                                                                             >  1:110188/1‑149 (MQ=255)
                                                      aaTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAgg                                                                             <  6:8706/148‑1 (MQ=255)
                                                       aTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                           >  5:312265/1‑149 (MQ=255)
                                                          tcAACCTCGCTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCgg                                                                        >  5:213111/1‑149 (MQ=255)
                                                               cTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTa                                                                   <  2:150469/149‑1 (MQ=255)
                                                               cTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTa                                                                   <  6:164794/149‑1 (MQ=255)
                                                                tCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGttt                                                                     >  6:159151/1‑146 (MQ=255)
                                                                    tCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAAc                                                              >  1:219746/1‑149 (MQ=255)
                                                                       ccGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActct                                                           <  5:159151/149‑1 (MQ=255)
                                                                        cgcgCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTg                                                          >  1:285478/1‑149 (MQ=255)
                                                                         gcgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGt                                                         <  7:248800/149‑1 (MQ=255)
                                                                          cgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTc                                                        <  2:285478/149‑1 (MQ=255)
                                                                             atcccGTCATCGATGAGAGCAGAATAAGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACTCTCTAAAGGCTCAAGTGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcacc                                                     <  5:266433/147‑2 (MQ=255)
                                                                             aaCCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                     <  7:78445/149‑1 (MQ=255)
                                                                               cccGGCTTAGATGTGAGCAGATTACTGCGCTGTGCGTTACCCCGAGTATGTGAAAAGCCACTCGCTAAAGTCTCAAGGGGCTCCTTGGGGTGCGTGGATCTCACTTGCTCGATATCAAACAGGCGCGGTTTTAGAAACTCTGTCACAGa                                                   <  6:229559/149‑1 (MQ=255)
                                                                                ccGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGaaa                                                               >  7:394615/1‑136 (MQ=255)
                                                                                ccGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGaaa                                                               <  8:394615/136‑1 (MQ=255)
                                                                                ccGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGaa                                                  <  6:312265/149‑1 (MQ=255)
                                                                                 cgtcATCGATTAGAGCAGAATACTGCGCAGTGCGTTACCCAGAGTATGGCTATAGCCACGCTCTAAATCCTCAATGGTCACCTTGGCGTGCTTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAAc                                                 <  4:186380/146‑1 (MQ=255)
                                                                                     aTCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActc                                                            <  1:170787/134‑1 (MQ=255)
                                                                                     aTCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActc                                                            >  2:170787/1‑134 (MQ=255)
                                                                                     aTCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTg                                             >  4:12356/1‑149 (MQ=255)
                                                                                      tCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTg                                             >  8:123380/1‑148 (MQ=255)
                                                                                      tCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGc                                            <  8:108211/149‑1 (MQ=255)
                                                                                        gATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCCCCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAt                                          >  4:364177/1‑149 (MQ=255)
                                                                                         aTGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAtt                                         <  4:147519/149‑1 (MQ=255)
                                                                                          tGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATtg                                        >  5:104563/1‑149 (MQ=255)
                                                                                           gagaGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATc                                                                                    >  6:207548/1‑104 (MQ=255)
                                                                                            agagCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATtgtg                                      <  6:172909/149‑1 (MQ=255)
                                                                                              agCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCACCCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTc                                    >  1:293808/1‑149 (MQ=255)
                                                                                                     tACGGCGCAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                             <  6:376303/149‑1 (MQ=255)
                                                                                                       cGGCTCAGTGCGTTACCCAGAGTATGGCTAAAGCCACGCTCTAAAGGCTCAATGGTCTCCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCttt                            <  2:114711/148‑1 (MQ=255)
                                                                                                          cgcAGTGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTa                        <  6:116491/149‑1 (MQ=255)
                                                                                                               tGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAg                   <  2:219746/149‑1 (MQ=255)
                                                                                                               tGCGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAg                   <  6:213111/149‑1 (MQ=255)
                                                                                                                 cGTTACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCt                 >  4:286843/1‑149 (MQ=255)
                                                                                                                   ttACCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCtt                >  2:129648/1‑148 (MQ=255)
                                                                                                                     aCCCAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTAc             >  1:237954/1‑149 (MQ=255)
                                                                                                                      cccAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCTAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTAc             <  6:67080/148‑1 (MQ=255)
                                                                                                                      cccAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt            >  3:62838/1‑149 (MQ=255)
                                                                                                                       ccAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACtt           >  6:18353/1‑149 (MQ=255)
                                                                                                                        cAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTGAGCTTTACtc           >  7:362084/1‑147 (MQ=255)
                                                                                                                        cAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTg          <  6:62257/149‑1 (MQ=255)
                                                                                                                        cAGAGTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTg          <  2:273894/149‑1 (MQ=255)
                                                                                                                          gagTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGTACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGa        <  4:373570/149‑1 (MQ=255)
                                                                                                                            gTATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGt      >  1:140261/1‑149 (MQ=255)
                                                                                                                             tATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTca                                                       >  7:203860/1‑99 (MQ=255)
                                                                                                                             tATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTca                                                       <  8:203860/99‑1 (MQ=255)
                                                                                                                             tATGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTa     <  5:18353/149‑1 (MQ=255)
                                                                                                                               tGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCttt               <  1:270262/137‑1 (MQ=255)
                                                                                                                               tGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCttt               >  2:270262/1‑137 (MQ=255)
                                                                                                                               tGGCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTATGGTACTAAGCTTTACTTGGAGTaaa   <  2:110188/149‑1 (MQ=255)
                                                                                                                                ggCAAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAg  >  2:349988/1‑149 (MQ=255)
                                                                                                                                   |                                                                                                                                                 
CAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAG  >  NC_000913/3440793‑3441069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: