Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913832,3640AG20.0% 62.7 / 10.5 32intergenic (+128/+104)rhlE/ybiAATP‑dependent RNA helicase/DUF1768 family protein
Reads supporting (aligned to +/- strand):  ref base A (11/13);  new base G (2/4);  total (14/18)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAA  >  NC_000913/832220‑832497
                                                                                                                                                |                                                                                                                                     
cTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGc                                                                                                                                   >  5:83930/1‑149 (MQ=255)
    cGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGttt                                                                                                                               <  6:83930/149‑1 (MQ=255)
              tAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAAATTGCATGCTTTTGTAGGCCGGATGCGGCGTAAAcgccttatc                                                                                                                     <  1:26849/149‑6 (MQ=25)
                aTCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                   <  3:45676/149‑1 (MQ=255)
                     ttATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                 <  1:20590/146‑1 (MQ=21)
                          ccGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATGCGGCGTAAAcgccttatc                                                                                                                     <  8:107435/137‑6 (MQ=21)
                          ccGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATGCGGCGTAAAcgccttatc                                                                                                                     >  7:107435/1‑132 (MQ=21)
                            gggCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCacaac                                                                                                       <  5:43954/149‑1 (MQ=255)
                              gCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCAcaacaa                                                                                                     <  7:98672/149‑1 (MQ=255)
                                                   atgaACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTgag                                                                                 <  5:104455/148‑1 (MQ=255)
                                                            cGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAAttt                                                                                    <  5:179698/136‑1 (MQ=255)
                                                            cGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAAttt                                                                                    >  6:179698/1‑136 (MQ=255)
                                                                              gcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                          <  5:25564/80‑1 (MQ=255)
                                                                              gcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                          >  6:25564/1‑80 (MQ=255)
                                                                                  cGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGcc                                                 >  3:142934/1‑149 (MQ=255)
                                                                                   gCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGcc                                                 >  5:23467/1‑148 (MQ=255)
                                                                                        tCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAg                                           >  2:5467/1‑149 (MQ=255)
                                                                                                       ctctGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTAc                            <  6:23467/149‑1 (MQ=255)
                                                                                                          tGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGt                               >  3:544/1‑143 (MQ=255)
                                                                                                          tGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGt                               <  4:544/143‑1 (MQ=255)
                                                                                                                 tatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGtt                                                         >  5:121836/1‑110 (MQ=255)
                                                                                                                 tatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGtt                                                         <  6:121836/110‑1 (MQ=255)
                                                                                                                 tatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCtata                   <  4:142934/148‑1 (MQ=255)
                                                                                                                  atTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGc                                          >  2:48188/1‑124 (MQ=255)
                                                                                                                  atTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGc                                          <  1:48188/124‑1 (MQ=255)
                                                                                                                   ttGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGtttt                                                       >  3:110870/1‑110 (MQ=255)
                                                                                                                   ttGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGtttt                                                       <  4:110870/110‑1 (MQ=255)
                                                                                                                           gCATGCTTTTGTAGGCCGGAT‑AGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGtt                                                         <  3:84741/99‑1 (MQ=255)
                                                                                                                           gCATGCTTTTGTAGGCCGGAT‑AGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGtt                                                         >  4:84741/1‑99 (MQ=255)
                                                                                                                                 ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCaa  >  6:128555/1‑149 (MQ=255)
                                                                                                                                        tgCCGGATGCGGCGTAAACGCCTTATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATaa                                            <  7:18593/99‑1 (MQ=37)
                                                                                                                                        tgCCGGATGCGGCGTAAACGCCTTATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATaa                                            >  8:18593/2‑100 (MQ=37)
                                                                                                                                                |                                                                                                                                     
CTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAA  >  NC_000913/832220‑832497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑