Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 564,107 IS2 (+) +5 bp coding (370‑374/633 nt) sfmZ ← response regulator family protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)34 (0.390) 31/284 1.4 97.0% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 564111 1 (0.010)coding (370/633 nt) sfmZ response regulator family protein
* ? NC_000913 = 382590NA (NA)71 (0.810) 52/286 0.5 98.6% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 564107 = 1 (0.010)coding (374/633 nt) sfmZ response regulator family protein

GAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATATTTTTCTCTTTAACA  >  NC_000913/382447‑382738
                                                                                                                                               |                                                                                                                                                    
gAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTAc                                                                                                                                                 <  5:132460/149‑1 (MQ=32)
gAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTAc                                                                                                                                                 >  2:8552/1‑149 (MQ=32)
   ccTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTa                                                                                                                                              >  3:36733/1‑149 (MQ=34)
        cAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAg                                                                                                                                          <  2:205016/148‑1 (MQ=38)
            ggCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCa                                                                                                                                       <  8:292983/147‑1 (MQ=40)
            ggCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCa                                                                                                                                       >  7:292983/1‑147 (MQ=40)
               gTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGCTAAGTGATAACAGATGTCTGGAAATAAAGGGGCAAATCCAATTACCTATCAGGCAGTTTg                                                                                                                                  <  5:339524/149‑1 (MQ=255)
                   gAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTgcgc                                                                                                                               <  8:26176/148‑1 (MQ=255)
                   gAGCATTATAACGAATGGCATCCGCATAGTGCGATGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTgcgc                                                                                                                               >  3:239344/1‑148 (MQ=255)
                      cATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTAGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt                                                                                                                           <  5:109618/149‑1 (MQ=255)
                      cATTATAACGAATGGCATCCGCATACTGCGCTGGGTTATCGCACGCCACGGGAATAGCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAAt                                                                                                                           <  3:287093/149‑1 (MQ=255)
                           taACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTa                                                                                                                      >  3:342072/1‑149 (MQ=255)
                            aaCGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTAc                                                                                                                     >  8:286564/1‑149 (MQ=255)
                                  tGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACAGGAcc                                                                                                               >  4:152372/1‑149 (MQ=255)
                                       tCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGttt                                                                                                                                   >  6:352335/1‑124 (MQ=255)
                                       tCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGttt                                                                                                                                   <  5:352335/124‑1 (MQ=255)
                                          gCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATaa                                                                                                       >  6:85686/1‑149 (MQ=255)
                                           cATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCACATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAAc                                                                                                      <  8:226707/149‑1 (MQ=255)
                                            aTAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACg                                                                                                     <  1:8552/149‑1 (MQ=255)
                                            aTAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGGTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTAGCTATCAGGCAGTATGCGCCAATCACTACCGGACCCTGAATTACg                                                                                                     >  2:323960/1‑149 (MQ=255)
                                             tAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACgg                                                                                                    <  8:22148/149‑1 (MQ=255)
                                              aGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACgg                                                                                                    >  5:169330/1‑148 (MQ=255)
                                                  cgcTGGGTTATCGCTCGACACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCa                                                                                               >  2:330439/1‑149 (MQ=255)
                                                   gcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCAt                                                                                              <  6:169330/149‑1 (MQ=255)
                                                         ttATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACaaa                                                                                        >  8:117205/1‑149 (MQ=255)
                                                             cgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTACAGGGTGACGTGATATCAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATAc                                                                                     <  2:13574/148‑1 (MQ=25)
                                                              gctctcCACGGGAATATCTGCTGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGTGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCTCTACCGGACGCTGAATAACGGCTCCATGACAAATACgg                                                                                   <  5:325840/149‑1 (MQ=21)
                                                              gctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACgg                                                                                   <  4:36733/149‑1 (MQ=255)
                                                                      cGGGAATATCTGCTGCAGCGGTCTTTTAATGGGTTAATTGATATCAGATGTCTGGAAATATAGGGGCAAATCCTATTACCTATGAGTCAGTTTGCGGCTATCTCTACCGTACACTTAATAACGGCACCATGACAAATACGGGTATTcgc                                                                           <  1:214508/149‑1 (MQ=21)
                                                                          aaTATCTGCGGCAGCGGGCTTGTAATGGCTTAAGTGATAACAGATGTCTGGAAATATAGGGTCAAATCCAATTACCTATCACGCAGTATGCGCCAATCCCTACCGGACACTGAATAACGTCCCCAAGACAAATACTGGTATTAGCGcc                                                                        >  1:60379/1‑148 (MQ=21)
                                                                            tatCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGa                                                                      >  2:12573/1‑148 (MQ=255)
                                                                            tatCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGAt                                                                     <  7:59234/149‑1 (MQ=255)
                                                                               cTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaa                                                                   >  6:335567/1‑148 (MQ=255)
                                                                                 gCGGCAGCGGGCTTGTAATGGGTAAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACACTGAATAACGTCACCATGACAAATACGGGTATTCGAGCCGATaacaa                                                                >  3:15160/1‑149 (MQ=255)
                                                                                  cgtcAGAGGGATTGTAATGTGTTTAGTGATAACATATGTCTGGAAATTTAGGGGAAAATACAATTACCTATCAGGCAGTTTGCGCCAATCACTACCTGACCCTGAATTACGGCAGGATGACAAATACGGGTATTCGGGCCGATaac                                                                  <  2:60379/143‑1 (MQ=255)
                                                                                  cGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCACGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaac                                                                  <  3:348185/146‑1 (MQ=255)
                                                                                       gCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCAc                                                          <  5:85686/149‑1 (MQ=255)
                                                                                       gCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCAc                                                          <  7:355701/149‑1 (MQ=255)
                                                                                             ttGTAATGGGTTAAGTGATAACAGATGTCTGGAATTATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGAACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAg                                                    >  3:70620/1‑149 (MQ=255)
                                                                                                  aTGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTtc                                                <  3:152372/148‑1 (MQ=255)
                                                                                                     ggTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTtcatc                                             <  4:239344/148‑1 (MQ=255)
                                                                                                      gTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTAACTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGAACCATGACAAATAAGGGTAATCGCGCCGATAACAACTACACCTGCAGTATCATCaa                                           >  4:42040/1‑149 (MQ=38)
                                                                                                       ttAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACATGAAGTTTCATCaa                                           >  7:324871/1‑148 (MQ=255)
                                                                                                           gTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCaataat                                       >  3:130582/1‑148 (MQ=255)
                                                                                                              aTAACAGATGTCGGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCGAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTAcc                                   >  1:205628/1‑149 (MQ=255)
                                                                                                                 aCAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTgg                                >  6:167998/1‑149 (MQ=255)
                                                                                                                 aCAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTg                                 <  1:330439/148‑1 (MQ=255)
                                                                                                                  cAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGt                               >  1:164917/1‑149 (MQ=255)
                                                                                                                   aGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTc                              <  5:335567/149‑1 (MQ=255)
                                                                                                                       gTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATAGGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGc                           >  3:47335/1‑148 (MQ=255)
                                                                                                                        tCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCaa                         >  3:342882/1‑149 (MQ=255)
                                                                                                                          tGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCTATAATTTCCTGGTCTGCAATc                       >  1:247371/1‑149 (MQ=255)
                                                                                                                            gAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAAt                        <  7:286564/146‑1 (MQ=255)
                                                                                                                            gAAATATAGGGGCAAATCCAATTACCTATCAGGCAGGTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGCGGATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTg                     <  3:42040/149‑1 (MQ=255)
                                                                                                                                     gggCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTCGTCTGCAATCTGAATAttttt            <  3:16070/149‑1 (MQ=255)
                                                                                                                                          aaTCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATATTTTTCTCtt       <  2:164917/149‑1 (MQ=255)
                                                                                                                                           aTCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATATTTTTCTCtt       >  7:55149/1‑148 (MQ=255)
                                                                                                                                               aaTTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATATTTTTCTCTTTAACa  >  4:292386/1‑149 (MQ=255)
                                                                                                                                               aaTTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCACCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATATTTTTCTCTTTAACa  >  1:300807/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
GAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATATTTTTCTCTTTAACA  >  NC_000913/382447‑382738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.