Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,880,008 | G→A | 5.7% | intergenic (‑60/+113) | yeaR ← / ← leuE | DUF1971 family protein, nitrate‑inducible/leucine efflux protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,880,008 | 0 | G | A | 5.7% | 231.3 / 3.4 | 70 | intergenic (‑60/+113) | yeaR/leuE | DUF1971 family protein, nitrate‑inducible/leucine efflux protein |
Reads supporting (aligned to +/- strand): ref base G (35/31); new base A (2/2); total (37/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01 |
CGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTC > NC_000913/1879724‑1880286 | cGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTg > 2:508192/1‑289 (MQ=255) cccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCACCGGGCGCGATCCTTTATCAAGATGACGATCGAATATTCCGGCAGGTGCAGTTTGTGTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCGGTGGCATTTGAAGCATCCTTTTCCCTCCTTCGGTGCATGCGCTGTAAACGGTTTTTTCCAGCCGTTTCAGGGTCCGCCTGATAATTTGCa > 2:479821/1‑289 (MQ=255) ccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGGTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCAt < 1:612921/289‑1 (MQ=255) gagaaGGATCACCTGATCAGGCTCTGCACTGTGGTCATCGGCGTAGCCGAGATATTGGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAAt < 2:673591/286‑1 (MQ=255) aTAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAAt < 2:258060/288‑1 (MQ=255) tGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGt < 1:496681/289‑1 (MQ=255) ctGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAg < 1:278660/289‑1 (MQ=255) gtgtTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCGGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCata > 1:195795/1‑289 (MQ=255) gtgtTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCata > 1:572739/1‑289 (MQ=255) tgtTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCata > 1:5990/1‑288 (MQ=255) tgtTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCata > 1:17533/1‑288 (MQ=255) atcaGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCg < 1:479821/289‑1 (MQ=255) atcaGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCg < 1:508192/289‑1 (MQ=255) cGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAACCGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCgaagaa > 2:173104/1‑289 (MQ=255) cGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTTCAGCCGTTTCTGGGTACGCCTGGTACTTTGCATTTTAAATCCCATTTATTGGTTACTTTTTAGGCACATTTCATCgaaaca > 2:522915/1‑286 (MQ=255) cGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCata > 2:397043/1‑278 (MQ=255) cGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCata < 1:397043/278‑1 (MQ=255) cGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCgaagaa > 1:552640/1‑289 (MQ=255) cGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCgaagaa > 1:671747/1‑289 (MQ=255) gTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCgaagaa < 2:94476/288‑1 (MQ=255) tAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCTTGATAATTTGCATTTTAAATACCATTTATTGTTTCCTTTTTAGCACAATATCAGCGAAGAATc > 2:2308/1‑289 (MQ=255) cgtgaTATTGTACCGCCCCATGCATAACGGAAAGTCGTTGGTGAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCATTTTGTTTATTCCAGAAAGGCGTTGACCGCGTATGAATATAATTCTGTGGGATTTTAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAgg < 2:478166/285‑1 (MQ=255) cGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTCCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAgg < 1:621758/288‑1 (MQ=255) cGAGATATTTGACCGCCACATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAgg < 1:193764/288‑1 (MQ=255) gagaTATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGgga < 2:631187/289‑1 (MQ=255) ttGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAgg > 2:127112/1‑280 (MQ=255) ttGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAgg < 1:127112/280‑1 (MQ=255) gCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATgg > 1:517441/1‑289 (MQ=255) aaCGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAggg < 1:13394/264‑1 (MQ=255) aaCGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAggg > 2:13394/1‑264 (MQ=255) aCGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTa < 2:694928/224‑1 (MQ=255) aCGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTa > 1:694928/1‑224 (MQ=255) aCGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGAc > 1:256084/1‑289 (MQ=255) agcgcttatggggTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACa > 2:310982/11‑288 (MQ=255) cGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCggg < 2:193004/288‑1 (MQ=255) tGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTg > 1:30247/1‑289 (MQ=255) aTGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGAATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACACCGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGc > 2:506160/1‑289 (MQ=255) tGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCggg < 2:17533/287‑1 (MQ=255) gTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGc > 1:553865/1‑158 (MQ=255) gTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGc < 2:553865/158‑1 (MQ=255) gTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACACCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGa > 1:397207/1‑289 (MQ=255) gTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACACCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGAACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCTGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGa > 1:397200/1‑289 (MQ=255) cGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACACCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACa < 2:397207/289‑1 (MQ=255) cGGCAGGTGACGTTTATTTATTCCAGAAAGGCGTTTAGCGCGGATGAATATAATTCTGTTGGATTTGAATCATCCTTTGCGCTCCTTCGGTGAATGCGCTGAAAACGGTGTATTCCAGCCGTTTCAGGGTACACCTGGTAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGAGGTGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACa < 2:397200/289‑1 (MQ=255) gCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATc > 1:58180/1‑289 (MQ=255) tCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAgg < 2:243882/180‑1 (MQ=255) tCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAgg > 1:243882/1‑180 (MQ=255) gTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGc < 2:552640/288‑1 (MQ=255) gAATATATTTCGTTGGGATTTGAAGCATCCGTTTCCCTCCTTCGGTGAATGCGCTGAAAACTGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTTGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTgg < 2:485313/289‑1 (MQ=255) gAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCGGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTgg < 2:195795/289‑1 (MQ=255) gAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTgg < 2:671747/289‑1 (MQ=255) gAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGAGTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTgg < 2:572739/289‑1 (MQ=255) aaTTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACTTCAAACCAATCAGTGAGTTCCCAACTTTAGCCAGTTTCTTTTTGGTACGTa > 1:27232/1‑289 (MQ=255) tgtgGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAAt < 1:125455/99‑1 (MQ=255) tgtgGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAAt > 2:125455/1‑99 (MQ=255) tgGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACt > 1:698957/1‑288 (MQ=255) tgGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACt > 1:136351/1‑288 (MQ=255) tttGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTg > 1:721111/1‑288 (MQ=255) tttGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTg > 2:138159/1‑288 (MQ=255) tCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCAc < 1:2308/289‑1 (MQ=255) tCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCAc < 2:256084/289‑1 (MQ=255) tCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCAc < 2:58180/289‑1 (MQ=255) tCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCAc < 1:310982/289‑1 (MQ=255) cctTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCTGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATc > 1:365441/1‑289 (MQ=255) cctTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATc > 1:324288/1‑289 (MQ=255) ctTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCa > 1:13032/1‑289 (MQ=255) ttCGGTGAATGCGCCGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGGAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAACCCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTCTTTGGTACGTATGTACTGCGGTACACAAGCACCATATATAATAAg > 2:318936/1‑289 (MQ=255) tGAAATCGGTTTTTTCGAGCCGTTTCAGGGTACGACTTATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCAGATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCGGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGc < 2:5990/289‑1 (MQ=255) ccGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATTTAATCAGGAAGCTCAAATAGCAGAAACTAACCAGTTCCa > 2:703567/1‑288 (MQ=255) tCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTc > 1:713311/1‑288 (MQ=255) | CGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCAGGTGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGAAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGATATAATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTC > NC_000913/1879724‑1880286 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |