BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,413	A→C	9.4%	T86P (ACC→CCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,413	0	A	C	9.4%	551.0 / 2.3	266	T86P (ACC→CCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (94/131); new base C (3/38); total (97/169)
Fisher's exact test for biased strand distribution p-value = 6.51e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

GCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGG  >  NZ_CP034943/607275‑607552

   aCGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCgg                                                                                                                                         <  2:2211638/140‑1 (MQ=255)

   aCGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCgg                                                                                                                                         <  1:484398/140‑1 (MQ=255)

    cGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGACCAACCGGAGCGACGGGCGCCACGGGAGCTACCGGGGACGCCGGACCGGCCGGACCAACCggc                                                                                                                                        >  2:670019/1‑139 (MQ=255)

     gggAGCTACCGGCGCAGCTGGACCAGCCGGAGCTACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAACAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggag                                                                                                                                       <  2:3745644/140‑1 (MQ=255)

         gCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgac                                                                                                                                   >  2:3101054/1‑140 (MQ=255)

              cGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggag                                                                                                                              <  1:3476873/140‑1 (MQ=255)

               ggggAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCgga                                                                                                                                        >  1:1005825/1‑129 (MQ=255)

                 ggAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACGGGGGAAGCAGGACCAGCCGGCGCAACAGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGAGCAGCCGGACCAACCggagcgacaggagcgc                                                                                                                           >  1:2074672/1‑139 (MQ=17)

                 ggAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAACCCGGACCAGCCGGACCAACCggagcgacaggagcga                                                                                                                           >  1:3612574/1‑140 (MQ=255)

                  gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                                                                          <  2:3161457/140‑1 (MQ=255)

                  gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCTGACCCGCCGGACCAACCggagcgacaggagcgac                                                                                                                          <  1:2383192/140‑1 (MQ=255)

                  gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                                                                          <  1:1603622/140‑1 (MQ=255)

                  gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                                                                          <  1:2340797/140‑1 (MQ=255)

                   aaGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgaca                                                                                                                         >  1:3303838/1‑140 (MQ=255)

                       cggACCAGCCGGAGCGCCGGGAGCGACCGGAGCTACCGGCGACGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                                                                                     <  2:700795/139‑1 (MQ=255)

                          aCCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                                                                                  <  2:2612433/140‑1 (MQ=255)

                               ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACGAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                             >  2:2967705/1‑140 (MQ=255)

                               ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                             >  2:1191799/1‑140 (MQ=255)

                               ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                             >  2:1503555/1‑140 (MQ=255)

                               ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                             <  1:4747647/140‑1 (MQ=255)

                               ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                             >  2:3450188/1‑140 (MQ=255)

                               ccGGAGCGACGGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGAGGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                             <  2:3278626/140‑1 (MQ=255)

                                   aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCAGGACAAACCGGAGCGACGGGAGCGACCGGAGCTCCCGGGGCAGCCGGACCAGCCGTCCCAACGGGAGCGACCGGAGGGACCGGAGCGAGCGGGGGAg                                                                                                         >  1:1507091/1‑140 (MQ=14)

                                        cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCCCGGGAGCTACCGGGGAAGCCGGAGCAGCCGGCCCAACCGGAGCGACAGGAGCGCCAGGAGCTACCGGGGAAGCCggc                                                                                                    >  2:234313/1‑139 (MQ=255)

                                        cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCAACCGGAGCGACAGGAGCTACAGGAGCACCCGGGGCAGCCGGa                                                                                                    >  2:89685/1‑140 (MQ=14)

                                        cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCGGGa                                                                                                    >  2:3514854/1‑140 (MQ=255)

                                        cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCCGCCGGAGCTACCGGCGAAGCCGGa                                                                                                    >  2:835587/1‑140 (MQ=255)

                                        cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCCCCGGGAGCACCCGGGGAAGCCGGAGCAGCCGGACCAACCGGAGCGACAGGAGCGACCGGAGCGACCGGGGCAGCCGGa                                                                                                    >  2:276918/1‑140 (MQ=17)

                                         gggCGCGACCGGAGCCACCGGCGAAGCCGGCGCAGCGTGAGCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGGGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                   <  1:4088107/140‑1 (MQ=14)

                                         gggAGTGACAGGATGAACCGGGGAATCCGGACCAGCCGGCCCATCCTGAGCGACTGGAGCCACGGGAGCTACCGGTGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCAGGAc                                                                                                   <  2:2888846/140‑1 (MQ=14)

                                         gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                   <  1:1255010/140‑1 (MQ=255)

                                         cggagccacaggagccacaGGCGAACCCGGACCACCCGGACCACCCGGAGCGACGGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                   <  1:4086969/139‑1 (MQ=14)

                                                    gcgcgaCCGGGGACGCCGGACCAGCCGGACCACCCGGCGCGACGGGAGCCACGGGAGCTCCGGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcg                                                                                        <  2:4444066/135‑1 (MQ=17)

                                                    gAGCCACCGGGGAACCCGGACCCGCCGGACCACCCGGAGCGACTGGAGCCACGGGAGCTACCGGGGAAGCCGGTCCAGCGGGCCCACCCGGAGCCACTGGAGCTACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcg                                                                                        <  2:560936/140‑1 (MQ=14)

                                                       cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGCGCAGCCGGCGAAgccggagcggccggagcgccc                                                                                     >  2:1596176/1‑137 (MQ=14)

                                                         aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCgg                                                                                                                                         <  1:2211986/86‑1 (MQ=255)

                                                         aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCggagcgaccgg                                                                                   >  2:2392482/1‑140 (MQ=255)

                                                            ggcgAAGCGGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagc                                                                                <  2:1135446/137‑1 (MQ=255)

                                                                ctgccggaccagcgggaccaACCGGAGCGACTGGACCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgaca                                                                            <  2:81747/138‑1 (MQ=255)

                                                                cagccggaccagccggaccACCCGGAGCGACGGGAGCGACGGGTGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgaca                                                                            <  2:4487947/139‑1 (MQ=255)

                                                                aaGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgaca                                                                            <  1:1707421/140‑1 (MQ=255)

                                                                  gccggaccagccggaccaTCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggag                                                                                                                              <  2:2419600/88‑1 (MQ=255)

                                                                  gccggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggag                                                                                                                              >  1:2419600/1‑88 (MQ=255)

                                                                  cccggaccagccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                                                                          <  1:730902/139‑1 (MQ=255)

                                                                    cggggcagccggaccaGCGGGAGCGAGTGGCGCCACTGGAGCTCCCGGCGAAGCAGGACCACCCGGACCCCCCGGAGGGACTGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCggcgcgacaggagcgacaggag                                                                        <  2:4574031/135‑1 (MQ=11)

                                                                    cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                               >  2:2056378/1‑117 (MQ=255)

                                                                    cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                               <  1:2056378/117‑1 (MQ=255)

                                                                       accagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcga                                                                     >  2:2156469/1‑140 (MQ=255)

                                                                        ccagcgggaccaAGGGGTGGGTGGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                    <  1:1093870/140‑1 (MQ=17)

                                                                        ccagccggacccCCCGGAGCGACGGGCGCCACGGGAGCTCCCGGGGACGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                    <  1:1075878/140‑1 (MQ=17)

                                                                        ccagccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                    <  1:275826/140‑1 (MQ=255)

                                                                        ccagccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                    <  2:1433241/140‑1 (MQ=255)

                                                                        ccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                    <  1:3226061/140‑1 (MQ=255)

                                                                        ccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                    <  1:4920902/140‑1 (MQ=255)

                                                                         cccccggaccaCCCGGACCCACGGGAGCCACGGGACCTACCGGCGCACCCGGACCAGCCGGACCCCCCGGAGCGACCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagccacgggagccaca                                                                   <  1:439134/137‑1 (MQ=17)

                                                                          ccccggaccaACCGGAGCGACGGGAGCAACGGGAGCTACCGGGTAAGCCGGACCAGCCGGCCCAACCGGATCGACCGGAGCGACTGGAGCTACGGGGGAAGCCGGACCAGCCGgagctaccggagcgacaggagcgacag                                                                  <  1:1959607/138‑1 (MQ=14)

                                                                          agccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGgagcgacaggagcgacaggagcgacag                                                                  <  1:1181728/140‑1 (MQ=255)

                                                                            ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGTACCACCAGTACCAACCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAACCCGGACCACCAGGcgcgtctggagcgagaggcgcgccaggc                                                                >  2:1869061/1‑139 (MQ=17)

                                                                               ccccaACGTGAGCGACGGGAGCCTCCGGAGCTACCGGGGTAGCCGGACCAGCCGGAGCAACGGGAGCGACTGGAGCGCCTGGAGCTCCCGGGGAAGCCGGACCAGCCGGCGCGACCGGAGCGACAGGAGCGACAGGAGCt                                                             <  2:4409370/138‑1 (MQ=11)

                                                                                    aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgac                                                                                                                                   >  2:2167397/1‑65 (MQ=255)

                                                                                    aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgac                                                                                                                                   <  1:2167397/65‑1 (MQ=255)

                                                                                    aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACCGGAGCTACCgg                                                        >  2:3569450/1‑140 (MQ=255)

                                                                                    aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCgg                                                        >  2:394001/1‑140 (MQ=255)

                                                                                    aCCCGAGCCACGGGTGCGCCGGGAGATAGCGGTTAAGCCGCAACAGCCGGACCACCCGGAGCGCCCGGAGCGACCGGAGCTACGGGTGAAGCCGGCCCAGCCGGAGCGACAGGAGCGACTGGAGCGACAGGAGCTACCgg                                                        <  2:609382/140‑1 (MQ=9)

                                                                                     cgggcgCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGCCACAGCCGGACCACCCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:3781786/135‑1 (MQ=14)

                                                                                     ccGGCGCGACGGGAGCCACGGGCCCTACCGGGGAAGCCGGCCCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:3911306/140‑1 (MQ=17)

                                                                                     ccGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       >  2:2218918/1‑140 (MQ=255)

                                                                                     ccGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       >  1:3414613/1‑140 (MQ=255)

                                                                                         aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   >  1:4367199/1‑140 (MQ=255)

                                                                                         aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   >  1:2574034/1‑140 (MQ=255)

                                                                                             aCGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGCGCGACAGGAGCTACCGGGGAAGCTgg                                               >  1:627007/1‑140 (MQ=255)

                                                                                              cGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                              <  1:512107/140‑1 (MQ=255)

                                                                                              cGGGAGCCACGGGAGCGACCGGGGAAGCCGGACCACCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                              <  1:3648870/140‑1 (MQ=255)

                                                                                              cGGGAGCCACCGGAGCTACCGGGGATGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                              <  2:3870797/140‑1 (MQ=14)

                                                                                               gggAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAc                                             <  1:840648/140‑1 (MQ=255)

                                                                                                    ccACGGGAGCTCCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt                                        <  1:21941/140‑1 (MQ=255)

                                                                                                    ccACGGGAGCGACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt                                        <  2:4060099/140‑1 (MQ=255)

                                                                                                      aggggAGCTCCCGGGGAAGCCGGACCATCCGGACCAACCGGAGCGACTGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTgg                                      <  2:3985641/138‑1 (MQ=14)

                                                                                                             cTACCGGGGCAGGCGGACCCGCCGGACCAACCGGAGCGACTGGAGCGACAGGTGCTACCGGGGAAGCCGGACCAGCCGGAGCGACTGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACg                               <  2:850704/140‑1 (MQ=14)

                                                                                                              tACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                                                                          <  1:1342258/96‑1 (MQ=255)

                                                                                                              tACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                                                                          >  2:1342258/1‑96 (MQ=255)

                                                                                                               cccGGGGAAGCCGGACCAGCCGGACCCCCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             <  2:4608758/139‑1 (MQ=255)

                                                                                                               aCCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             <  2:1716112/140‑1 (MQ=255)

                                                                                                               aCCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             <  2:195779/140‑1 (MQ=255)

                                                                                                               aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTa                                                            >  2:4160358/1‑109 (MQ=255)

                                                                                                               aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTa                                                            >  2:1025617/1‑109 (MQ=255)

                                                                                                               aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTa                                                            <  1:4160358/109‑1 (MQ=255)

                                                                                                               aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTa                                                            >  2:3328596/1‑109 (MQ=255)

                                                                                                               aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             <  2:3802468/140‑1 (MQ=255)

                                                                                                                 cGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAg                           <  1:4483888/140‑1 (MQ=255)

                                                                                                                    ggAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGCCCGGGGCGACAGGCGGGACAGGGGCTACCGGGGACGCTGGACCAGCTGGAGCGCCGGGAGCgc                        >  1:2875469/1‑139 (MQ=14)

                                                                                                                     gAAGCCGGCCCACGCGGACGTACCGGAGCGAGAGGAGCGACAGGCGCTACCGGTGACGCCGGACCAGCCTGAGCGACAGGAGCGACAGGTGCGACTGGAGCTAGCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                       <  2:3004203/140‑1 (MQ=17)

                                                                                                                        gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgac                                                                                      <  1:312571/74‑1 (MQ=255)

                                                                                                                        gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                    >  2:4724387/1‑140 (MQ=255)

                                                                                                                        gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                    >  2:4900000/1‑140 (MQ=255)

                                                                                                                             accagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCGACCGGAGCTACCGGGGAAGCAGGAGCAGCTGGGGCGACGGGAGCGCCAGGGGcgc               >  2:105634/1‑138 (MQ=17)

                                                                                                                             accagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGCGCtc               >  2:2715874/1‑139 (MQ=255)

                                                                                                                                  ccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCggg          <  1:2967705/140‑1 (MQ=255)

                                                                                                                                          aCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCgg  >  1:282593/1‑140 (MQ=255)

                                                                                                                                          aCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCgg  >  1:3348685/1‑140 (MQ=255)

GCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGG  >  NZ_CP034943/607275‑607552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`