BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,702	C→T	19.9%	A182V (GCC→GTC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,702	0	C	T	19.9%	443.8 / 116.1	225	A182V (GCC→GTC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base C (93/86); new base T (42/2); total (136/89)
Fisher's exact test for biased strand distribution p-value = 9.64e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 226 total aligned reads displayed.

CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGG  >  NZ_CP034943/607567‑607840

       cacaGGGGCTACTGGGGAAGCCGGAGCCGCCGGAGCGCCAGGAGCGACCGGGGACGCCGGACCAGCCGGGGCGACAGGCGGGGGCGGGGCAGCCGGACCAACCGGAGCGACAGGAGCGACGGGGGCAGCCGGAccgggcg                                                                                                                                 >  1:516161/1‑135 (MQ=14)

       cacaGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCGGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCg                                                                                                                                 >  2:651091/1‑140 (MQ=255)

       cacaGGGGCGACTGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACCGGAGCTCCCGGGGCCGCCGGCCCACCCGGCGCGCCCGGAGCTCCCGGGGCCGCCGGCCGAGCCg                                                                                                                                 >  2:3259339/1‑140 (MQ=14)

              gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCAACCGGCGAGTCCGGACAATCCGgcgctcc                                                                                                                          >  1:2491382/1‑137 (MQ=11)

                tACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAg                                                                                                                        >  1:4620104/1‑140 (MQ=255)

                tACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCCACCGGAGCCGCAGGAACAGCCGGAGCgcgct                                                                                                                        >  2:1521112/1‑136 (MQ=14)

                 aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACTGGAGCTACAGGGGAAGCCGGACCACCCGGAGCGCCAgg                                                                                                                       >  2:4584681/1‑140 (MQ=255)

                 aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                       >  1:951296/1‑140 (MQ=255)

                 aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                       <  2:4620104/140‑1 (MQ=255)

                 aCTGGGGAAGCCGGACCAGCCGCAGCGACAGGAGCTACCGGGGAAGCCGCACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGACGGAGCGACAgg                                                                                                                       >  1:3092658/1‑140 (MQ=255)

                   tGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                                     >  2:4838243/1‑140 (MQ=40)

                   ccgcgcaGCCGCACCACCCGGAGCGACAGGAGCCACCGGCGACCCCGGCGCCGCCGGAGCCGCCGGAGCTCCCGGAGCTCCCGGAGCCACCGGAGCGACAGGGGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                                     <  1:219764/134‑1 (MQ=9)

                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACCGGGGCCACAGGCGCAGCCGGAGCGACAGGAGCCAca                                                                                                                >  2:688723/1‑139 (MQ=11)

                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCAGCGCAGCCGGCCCAGCCGGAGCGACCGGAGCtcacgg                                                                                                              >  1:282252/1‑135 (MQ=11)

                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCgg                                                                                                              >  2:1421592/1‑140 (MQ=255)

                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAACCGGACCAGCCGGAGCTAAAGGAGCTACCGGGGAAGCCGGAACAACCGGAGCGACAGGAGCTACCGGGGAAGCCGCACCATCCGGAGGGACAGGAGCTACCgg                                                                                                              >  1:1117376/1‑140 (MQ=14)

                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGCAg                                                                                                         >  2:2236103/1‑140 (MQ=255)

                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         >  2:2384181/1‑140 (MQ=255)

                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         <  1:4076043/140‑1 (MQ=255)

                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGGCGGAGCGCCAGGAGCGACCGGCGAGGCCGGAGCAGCCGGAGCTACAGGAGCCACCggagccg                                                                                                         >  2:1927366/1‑135 (MQ=11)

                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGATACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         <  1:2421870/140‑1 (MQ=255)

                                ccgcccGGATCGACAGGCGCTTCCGGAGAAGCCGGAGCCACCGGAGCGACAGGTGCTACCGGTGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                        <  2:2583550/136‑1 (MQ=14)

                                cccgCCGGAGCGACCCGAGCGACGGGCGATGCCGGCCCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGc                                                                                                        <  2:4124175/137‑1 (MQ=14)

                                   gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGCCGGGAGCGACAGGCGCCGCCGGACCACCCGGCGCGACAGGAGCTACCTGGGCCGCCgg                                                                                                     >  2:4959455/1‑140 (MQ=14)

                                    ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                                  >  2:2916657/1‑110 (MQ=40)

                                    ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                       >  1:115386/1‑121 (MQ=40)

                                    ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                       <  2:115386/121‑1 (MQ=40)

                                     cGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                   <  1:4838243/140‑1 (MQ=255)

                                        aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGACGGAGCGGCAGGAGCTCGCGGGGAAGCCGGAccgc                                                                                                >  1:1421038/1‑138 (MQ=14)

                                         gCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                                               <  1:1783945/140‑1 (MQ=255)

                                         gCGACAGGCGCTACCGGCGAAGCCGGACCCGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                                               <  1:4575096/140‑1 (MQ=255)

                                          cGACAGGAGCTCCCGGGGATGCCGGTCCATCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                              <  1:4822711/140‑1 (MQ=14)

                                                  gCTCCCGGGGAAGCCGGAGCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                      <  1:2392482/140‑1 (MQ=255)

                                                  gCTCCCGGCGAAGCCGGACCCGCCGGCGCGCCAGGACCTACGCGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                      <  1:2683824/140‑1 (MQ=14)

                                                  gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAACCAGGCCCAGCCGGAGCGACAGGAGCAACGGCGGAAACAGGCACAGCCGCAGCGAc                                                                                      >  2:1550876/1‑140 (MQ=11)

                                                  cccaCCGGCGCAGCCGGACCATCCGGAGCCCCAGGAGCTACCGGGCAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                      <  1:4191091/137‑1 (MQ=14)

                                                         cgccaGCCGGACCCCCCGGAGCGACAGGCGCTCCCGGGGAAGCCGGACCACCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGcc                                                                               <  2:3451431/136‑1 (MQ=11)

                                                           gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCcac                                                                             >  1:831362/1‑140 (MQ=255)

                                                           gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                             >  1:4659351/1‑140 (MQ=255)

                                                           gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                             >  1:2142236/1‑140 (MQ=255)

                                                           ccccccGCACCACCCGCACCGACCGGAGCTCCCGGCGAACCCGGACCCACCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                             <  2:277412/136‑1 (MQ=14)

                                                            cggCCGGACCCCCCGGACCGACAGGCTCTAGCGGCGCACCCGGACCACCCGGAGCGACAGGAGCTTCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                            <  1:2791106/138‑1 (MQ=14)

                                                            aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                            <  1:4260566/140‑1 (MQ=255)

                                                             aGCCGGACCAGCCGGAGCGACCGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAg                                                                           <  1:2983509/140‑1 (MQ=255)

                                                              gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                          >  2:3126558/1‑140 (MQ=255)

                                                              gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGACGGAGCGACAGGAGCTCCCGGGGCCGCCGGACCAACCGGAGCGACGGGAGCGCCAgg                                                                          >  2:751189/1‑140 (MQ=14)

                                                              gCCGGACCACCCGGAGCCCCAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                          <  2:408639/140‑1 (MQ=255)

                                                              cccGGACCAGCCGGACCGCCTGGAGCTAACGGGGAAGCCGGAACATCCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                          <  1:1038704/139‑1 (MQ=14)

                                                                cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                       <  2:599248/93‑1 (MQ=37)

                                                                cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                       >  1:599248/1‑93 (MQ=37)

                                                                    ccAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTAc                                                                    <  1:3126558/140‑1 (MQ=255)

                                                                            aGCGACAGGCGCTCCCGGGGACGCCGGACCAACCGGAGCCACAGGCGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                            <  1:3688209/140‑1 (MQ=17)

                                                                            aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                            <  1:1702082/140‑1 (MQ=255)

                                                                            aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGCCCCAGGACCAACAGCAGCGCCGGGAGCCACAGCCGCGACTgtatacg                                                            >  2:3792476/1‑134 (MQ=14)

                                                                                  aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                      >  2:3608905/1‑140 (MQ=255)

                                                                                      gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGAGCGGAGCAACCGGGGCGGCCGGACCAACCGGACCGGCGGGAGCGACCGGGGCGTGGGGGGACGTCGGagcagc                                                  >  2:4519656/1‑140 (MQ=14)

                                                                                      gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGc                                                  <  2:2142236/140‑1 (MQ=255)

                                                                                         aCCGGGGCACCCCGACCAACCGGAGCGACAGGCGCTACCGGGGCAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                               <  1:3321630/140‑1 (MQ=18)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAACCGGAGCGACGGGAGCCACTGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGAc                                         >  1:402315/1‑140 (MQ=255)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCTGCCGGAGCGAc                                         >  2:896758/1‑140 (MQ=255)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGAc                                         >  1:3458977/1‑140 (MQ=255)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGAAGCCGGACCAACCGGAGCGCCGGGAGCCACAGGGGCTCCAGGGGCCGACGTACCAGCCGCAGCGAc                                         >  2:3245290/1‑140 (MQ=17)

                                                                                                  gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACa                                                                                                                         >  1:1666845/1‑57 (MQ=35)

                                                                                                  gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACAGGGCCCGTCGGACCAGCCGGAGCGACAgg                                      >  1:4370099/1‑140 (MQ=255)

                                                                                                  gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                      >  1:2359937/1‑140 (MQ=255)

                                                                                                  gCCGGACCAACCGGAGCGACAGGAGCAACCGGCGCCCCCGGCCCAGGCGCAGCGTCCGCAGCGACGGGGGAAGCAGTACCAGCCCACGCGACTGGTGCCACAGGGGCTAGAGGGCAAGTCGGACCAGCCGGAGCGACggg                                      >  1:1180121/1‑140 (MQ=255)

                                                                                                  cccgccccAACCCGAGCGACAGCAGCTACAGGGGCACCCGGACGATCCCGAGCGACAGGTGCTACCGGCGAAGCCGGACCATCACGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                      <  2:4033404/134‑1 (MQ=17)

                                                                                                  accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                      >  1:4898753/2‑140 (MQ=14)

                                                                                                  accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                      >  1:1981075/2‑140 (MQ=14)

                                                                                                    cGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCCAcagcag                                    >  2:1907386/1‑140 (MQ=14)

                                                                                                    cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAg                                    <  1:2911101/140‑1 (MQ=255)

                                                                                                    cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAg                                    <  1:4702077/140‑1 (MQ=255)

                                                                                                       aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGTGCGACTGGGGCCGTCGGACCAGCCGTAGCGACAGGAGCTa                                 >  2:2793537/1‑140 (MQ=255)

                                                                                                       aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCtc                                 >  1:70158/1‑139 (MQ=255)

                                                                                                        gcgacgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTAc                                >  1:4173635/7‑140 (MQ=17)

                                                                                                        gcgacgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTAc                                >  1:4126977/7‑140 (MQ=17)

                                                                                                        gcgacgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTAc                                >  2:283497/7‑140 (MQ=17)

                                                                                                        gcgacgggAGCCACAGGGGCTACAGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCTGACCAACCGGAGCGACGGCAGCCACAGGGGCTAATGGGTACGTCTGACCAGCCGGAGCGACAGGAGCTAc                                >  1:3898770/7‑140 (MQ=14)

                                                                                                           acgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGCCGTCGGACCAGCCGGAGCGACAGGCGCGCCCgg                             >  2:3934249/4‑140 (MQ=14)

                                                                                                            ccGGTGCTACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                            <  1:4254998/140‑1 (MQ=255)

                                                                                                            ccGGAGCGACCGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGACGGACCAGCCGGAGCGACAGGAGCTACCggg                            <  1:4536889/140‑1 (MQ=255)

                                                                                                            ccGGAGCGACAGGAGCTCCCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                            <  1:1086762/140‑1 (MQ=255)

                                                                                                            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCgg                                                                          >  2:3516379/1‑94 (MQ=18)

                                                                                                            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                          <  1:1761493/94‑1 (MQ=40)

                                                                                                            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                          >  2:1761493/1‑94 (MQ=40)

                                                                                                            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                            <  1:2236103/140‑1 (MQ=255)

                                                                                                                aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCCCAGGGGCTACTGGGGACGGCGGACCAGCCGCAGCGACAGGAGCTACCGGGGacg                        >  1:3940049/1‑138 (MQ=21)

                                                                                                                     cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                   >  2:2558844/1‑140 (MQ=255)

                                                                                                                           cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCGACTGGGGACGTCGGACCAGCCGGCGCCACAGGAGCTACCGGGGAAGCCGGACCAAcc             >  2:4258038/1‑140 (MQ=18)

                                                                                                                            tACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCg            >  1:3882521/1‑140 (MQ=255)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGCCGTCGGACCAGCCGGAGCGCCAGGAGCGACCGGGGAAGCCGGACCAACCgg           >  2:4657520/4‑140 (MQ=17)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcagc           >  2:3687927/4‑137 (MQ=255)

                                                                                                                             actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgc           >  1:3404385/4‑139 (MQ=255)

                                                                                                                               cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg         >  2:4277138/1‑140 (MQ=255)

                                                                                                                               cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg         >  2:3764903/1‑140 (MQ=255)

                                                                                                                                ggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGc        >  2:1665334/1‑140 (MQ=255)

                                                                                                                                  ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAACCGGACCACCAGCAGCGa      >  2:3078775/1‑140 (MQ=17)

                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc     >  1:1312429/1‑140 (MQ=255)

                                                                                                                                    ccgCCGGCCCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCCCCGGAGCGACGGGCGCCACTGGGGGTAGTGTGGACGTGGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa    <  1:1317893/138‑1 (MQ=14)

                                                                                                                                      gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                      <  1:4456198/104‑1 (MQ=255)

CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGG  >  NZ_CP034943/607567‑607840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`