Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,111,632 T→G 8.4% L576R (CTT→CGT)  addB → helicase‑exonuclease AddAB subunit AddB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,111,6320TG 8.4% 545.6 / 5.4 227L576R (CTT→CGT) addBhelicase‑exonuclease AddAB subunit AddB
Reads supporting (aligned to +/- strand):  ref base T (64/133);  new base G (28/2);  total (92/135)
Fisher's exact test for biased strand distribution p-value = 1.23e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

AATCATTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGG  >  NZ_CP034943/1111495‑1111771
                                                                                                                                         |                                                                                                                                            
    aTTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAtt                                                                                                                                        <  2:2418715/140‑1 (MQ=255)
     ttGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCtttaaatt                                                                                                                                       >  1:4661456/1‑134 (MQ=255)
     ttGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTCAttt                                                                                                                                       >  1:835096/1‑140 (MQ=255)
              agcagcaTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAAtt                                                                                                                              <  2:817716/140‑1 (MQ=255)
              agcagcaTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGACGCGGAGTCGCTTACATTTTCTCTAAtt                                                                                                                              <  2:1297091/140‑1 (MQ=255)
                  gcaTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGc                                                                                                                          <  2:3800714/140‑1 (MQ=255)
                     tCAGCAGGCGTGGGATGCAGTCATTCAGCTAGTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGccgg                                                                                                                       >  2:4576013/1‑138 (MQ=255)
                     tCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTGTGTTGAAATGATGGGGGATGATGAGATTGCTCTTGAGTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTACCTTTTCTGTAATTCCCCCTg                                                                                                                      >  2:3833312/1‑140 (MQ=255)
                        gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTgcgc                                                                                                                   <  2:215934/140‑1 (MQ=255)
                        gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTCCTTTTGTGTAATTACGCCGgcgc                                                                                                                   >  1:1725259/1‑140 (MQ=255)
                        gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTAATTTTGTGTAATTACGCCGgcgc                                                                                                                   >  2:1899044/1‑140 (MQ=255)
                        gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAAGGATGGAAGCCGGCGCGGAGTCGCGTTAATGTTCTCTACTTACGccgtggg                                                                                                                    >  1:4899885/1‑135 (MQ=255)
                        gcagGCATGGGATGCAGTCAGTCAGCTACTTGAGCAGTTTGTTGAAATGATGGGGGATGATGAGAGTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTgcgc                                                                                                                   <  1:4201650/140‑1 (MQ=255)
                             cGTGGGATGCAGTGATTCAGCTACTTGAGGAGTTTGTGGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGAc                                                                                                              <  1:3271633/140‑1 (MQ=255)
                             cGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTCTCTAATTACGCCGGCGCGTGAc                                                                                                              >  2:117589/1‑140 (MQ=255)
                             cGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGAc                                                                                                              <  1:2108143/140‑1 (MQ=255)
                             cGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTATCGAATTAAGCCGGCGCttttc                                                                                                              >  1:2475053/1‑137 (MQ=255)
                             cGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTATATAATTCAGCCGGCGCTTTAc                                                                                                              >  2:2696182/1‑140 (MQ=255)
                              gTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTAATTTTATATAAATACGCCTGCGCTTGaac                                                                                                             >  2:1588116/1‑138 (MQ=255)
                              gTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGAcc                                                                                                             <  1:2286019/140‑1 (MQ=255)
                              gTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGAcc                                                                                                             <  1:4359987/140‑1 (MQ=255)
                              gTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGAcc                                                                                                             <  1:74319/140‑1 (MQ=255)
                              gTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAATTTATGTAATTACGCAGGCGCGTGTcc                                                                                                             >  2:2986503/1‑140 (MQ=255)
                                 ggATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAgg                                                                                                          <  2:431652/140‑1 (MQ=255)
                                  gATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTGTCTAATTACGCCAGCGCTTTACCacgg                                                                                                         >  2:1349435/1‑137 (MQ=255)
                                      cAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTTTGTAAATAACCCCGCGGGTGACCAGGTGttt                                                                                                     >  1:1437823/1‑140 (MQ=255)
                                      cAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTGAACTTTTGTCTTATTACGCCTGGCGCTGGGCAGGTGttt                                                                                                     >  1:2980329/1‑140 (MQ=255)
                                      cAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTAATTTTGTCGAATTAAGCCAGCGCGTGAACCGGTGttt                                                                                                     >  1:2347294/1‑140 (MQ=255)
                                        gTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGt                                                                                                   <  1:2826041/140‑1 (MQ=255)
                                        gTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGt                                                                                                   <  2:3297354/140‑1 (MQ=255)
                                        gTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTCATTTTTTATAATTACGCCGGCGCTTGACCAGGTGttttt                                                                                                   >  1:1310738/1‑138 (MQ=255)
                                         tCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAATTTTTACAATTAAGCCTGCCCTTAGACAGgtgatatta                                                                                                  >  2:4042209/1‑134 (MQ=255)
                                            ttCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTGATTTTCTGTAATTACGCCAGCGCGTGAACAGGTGtttttgggg                                                                                               >  1:1047494/1‑134 (MQ=255)
                                            ttCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTtct                                                                                                                                    >  2:845367/1‑104 (MQ=255)
                                            ttCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTACGCCGGGGCTTGAGCAGGTGGTTGTCGGc                                                                                               >  2:1223788/1‑140 (MQ=255)
                                            ttCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAATTTCTCTAAATACGCCGGCGCTTGACCAGGTGTTTGTAGGc                                                                                               >  2:2050958/1‑140 (MQ=255)
                                                gCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAata                                                                                           <  1:4893872/140‑1 (MQ=255)
                                                   aCTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTTTATAATTACGCCTGGGCTTTTCCACGTGTTTTTGGGCAATATgg                                                                                        >  1:3589604/1‑140 (MQ=255)
                                                    cTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTATATAAATACGCCATCGCGTTACCCCGTGTTTGTGGGCAataaaga                                                                                       >  2:263538/1‑136 (MQ=255)
                                                     ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACCATTTATCATATTGCGCCGGGGCTTGACCATGTGTTTTTCGGCCatattggt                                                                                      >  2:2024312/1‑136 (MQ=255)
                                                     ttGAGGAGTTTGTTGAAATGATCGGGGATGATGAGATTTCTCTTGATTTGTTTCATCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                      <  2:4980373/140‑1 (MQ=255)
                                                          gagTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATc                                                                                 <  1:2545045/140‑1 (MQ=255)
                                                          gagTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATc                                                                                 <  1:3537580/140‑1 (MQ=255)
                                                            gTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCa                                                                               <  1:4388103/140‑1 (MQ=255)
                                                            gTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCa                                                                               <  2:3606347/140‑1 (MQ=255)
                                                             tttGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAATTTATATAAATACCCCGGCGCGTTACCCCGTGTGTGTCGGCAAAAATGATTTATGCAg                                                                              >  2:1850624/1‑140 (MQ=255)
                                                              ttgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTATAAATACGCCAGCGCTTTACCACGTGTTTTTCGGCAAAATTGATTTAtaccga                                                                             >  2:3414371/1‑135 (MQ=255)
                                                               tgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTAATTTTTTCTAATTGCGCCTGCGCGTTTCCAGGTGGTTTTGGGGACTATTGATTTATTCaaaa                                                                            >  1:4038608/1‑140 (MQ=255)
                                                               tgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAATTTATGTAATTACGCCAGAGCTTTACCAGGTGTTTTTCGGCAAAATTTATTTATACaaaa                                                                            >  2:2960941/1‑140 (MQ=255)
                                                               tgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTATCTAATTCAGCCCGCCCGTTACCACGGGTTTGTGGGCAATATGTATTTATCCACaa                                                                            >  2:4274450/1‑140 (MQ=255)
                                                               tgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAAATTTATAAAAATACCCCAGCCCTTGACAACGTGTTTTTCGGCAATATTGATTTaaacacaa                                                                            >  2:3178096/1‑133 (MQ=255)
                                                               tgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTAAATTTGTCTAATTACGCCAGCGCGTGACCACGTGTTTTTGGGCAATATTGATTTATACACaa                                                                            >  1:4873037/1‑140 (MQ=255)
                                                                gttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAAt                                                                           <  2:222694/140‑1 (MQ=255)
                                                                       tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                    <  1:858541/140‑1 (MQ=255)
                                                                       tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                    <  2:508391/140‑1 (MQ=255)
                                                                       tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                    <  1:1651419/140‑1 (MQ=255)
                                                                       tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCGGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                    <  1:385928/140‑1 (MQ=255)
                                                                       tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAAGTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                    <  1:641962/140‑1 (MQ=255)
                                                                       tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAGTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                    <  2:2901182/140‑1 (MQ=255)
                                                                       tgatgGGGGATGATGAGAGTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                    <  1:2701764/140‑1 (MQ=255)
                                                                        gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                   <  1:534487/140‑1 (MQ=255)
                                                                        gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                   <  1:1899044/140‑1 (MQ=255)
                                                                        gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                   <  1:3627336/140‑1 (MQ=255)
                                                                           gggggATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAAATTTGTATAAATGCGCCTTCGCTTGAACAGGTGTTTGTGGGGAATATTGATTTATACAGAATGTACGGCAAct                                                                >  2:1816198/1‑140 (MQ=255)
                                                                               gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATCTGGATTTATCCAGAATGTACGGCACCTCTTg                                                            <  2:4123834/140‑1 (MQ=255)
                                                                               gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                            <  2:1626113/140‑1 (MQ=255)
                                                                               gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                            <  1:322496/140‑1 (MQ=255)
                                                                                 tgttgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCa                                                          <  2:3984318/137‑1 (MQ=255)
                                                                                 tgatgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACTGCACCTCTTGCa                                                          <  2:1041919/140‑1 (MQ=255)
                                                                                  gatgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCGTGACCAGGTGTTTGTGGGCAATATGGATTTATACAGAATTTAAGGCACCACTTTCAc                                                         >  2:4717365/1‑140 (MQ=255)
                                                                                  gatgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCAGCCTTCGCGTTACCCGGTGTTTTTCGGCCAAATTGATTTATCCAAAATGTAAGGCCCCGCTTGCaa                                                         >  2:2614568/1‑139 (MQ=255)
                                                                                   atgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTTTAGAAATACCCCATCGCTTGTCCAGGTGTTTGTCGGCAAAATGGAATTATACACAATGTAAGGCACCTCTTTCAca                                                        >  2:3697159/1‑139 (MQ=255)
                                                                                   atgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTATATAATTAAGCCTGCGCTTTACCAGGGGGTTTTCGGCAAAATTTTTTTTTCAAAAATGTACGGCACCACTTGCAcc                                                        >  2:3383732/1‑140 (MQ=255)
                                                                                   atgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTCATTTTCTCAAAATCAGCCAGCGCGTGACCCGGTGGTTTTGGGCGATATGGGTTTTTACACAAATTAAGGCACCACTTTCaac                                                        >  1:4611509/1‑138 (MQ=255)
                                                                                    tgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTACTTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCt                                                       <  2:2335947/140‑1 (MQ=255)
                                                                                     gagaTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCtt                                                      <  2:3554159/140‑1 (MQ=255)
                                                                                        aTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTAAAATTTATCTAATTACCCCGGCGCTTGACCAGGGGTTTTTGGGCAATATGGATTTATACAGAAATTAACGCCCCACTTGGACCGTtgt                                                   >  2:3200116/1‑140 (MQ=255)
                                                                                          ttCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTCATTTTCTGTAATAACGCCGGCCCGTTACCCCGTGTTTTTGGGCCAAATTTATTTATCGACAATTTACGGCACCGCTTGGACCGTtgttg                                                 >  1:1795926/1‑138 (MQ=255)
                                                                                           tctcTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCt                                                <  1:2050958/140‑1 (MQ=255)
                                                                                             tctTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTGTATAAATTCGCCGGCGCGTGACCCGGTGGGTTTGGGGCATATTGATTTTTACAGAAATTAAGGCACCCCGTGGACCATTGtggtgg                                              >  1:414362/1‑140 (MQ=255)
                                                                                                       tttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacga                                    <  2:1555067/140‑1 (MQ=255)
                                                                                                        ttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacgac                                   <  1:1178704/140‑1 (MQ=255)
                                                                                                        ttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacgac                                   <  1:4295765/140‑1 (MQ=255)
                                                                                                        ttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacgac                                   <  1:3637524/140‑1 (MQ=255)
                                                                                                             cAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGt                              <  2:4459533/140‑1 (MQ=255)
                                                                                                              caaTGATGGAAGCCGGCGCGGAGTCGCTGACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATAGGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGtt                             <  2:3431780/139‑1 (MQ=255)
                                                                                                                atgatgGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTct                           <  2:489954/140‑1 (MQ=255)
                                                                                                                 tgatgGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTctg                          <  2:3878809/140‑1 (MQ=255)
                                                                                                                       aaGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCa                    <  1:4717365/140‑1 (MQ=255)
                                                                                                                       aaGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCa                    <  1:4573485/140‑1 (MQ=255)
                                                                                                                       aaGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCa                    <  1:1588116/140‑1 (MQ=255)
                                                                                                                       aaGCCGGCGCGGAGTCGCTTACATTTTCTCTAAGTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCa                    <  1:2986503/140‑1 (MQ=255)
                                                                                                                       aaGCCGGAGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCa                    <  1:2571947/140‑1 (MQ=255)
                                                                                                                           cGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGcc                <  2:4152311/140‑1 (MQ=255)
                                                                                                                               gcgGAGTCGCGTACATTTTCACTAATTCCGCCAGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTGGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCtga            <  2:84947/140‑1 (MQ=255)
                                                                                                                                     tCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCTGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGaaaa      <  1:3414371/140‑1 (MQ=255)
                                                                                                                                     tCGCTTACATTTTCTATAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGaaaa      <  1:1695245/140‑1 (MQ=255)
                                                                                                                                         ttACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGTATTTATCCAGAATTTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATggg  <  1:2965016/140‑1 (MQ=255)
                                                                                                                                         ttACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATggg  <  1:2958956/140‑1 (MQ=255)
                                                                                                                                         ttACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATggg  <  2:2427109/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                            
AATCATTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGG  >  NZ_CP034943/1111495‑1111771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: