Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,936,618 G→C 6.4% L3545V (CTG→GTG)  EO946_RS09735 ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,936,6180GC 6.4% 976.1 / 4.0 371L3545V (CTG→GTG) EO946_RS09735non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (125/207);  new base C (24/2);  total (159/212)
Fisher's exact test for biased strand distribution p-value = 3.02e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 371 total aligned reads displayed.

TGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAG  >  NZ_CP034943/1936483‑1936755
                                                                                                                                        |                                                                                                                                          
tGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGa                                                                                                                                        <  1:912776/140‑1 (MQ=255)
tGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGAGGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGACCTTGGTTACCGTCGTTTTTGGAAGTGCCTCGCCTCCAATTAACAGATGa                                                                                                                                        <  1:1546390/140‑1 (MQ=255)
  aaCAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACg                                                                                                                                      <  1:3951984/140‑1 (MQ=255)
  aaCAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACg                                                                                                                                      <  2:4802376/140‑1 (MQ=255)
  aaCAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACg                                                                                                                                      <  1:4265158/140‑1 (MQ=255)
  aaCAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACg                                                                                                                                      <  1:4640476/140‑1 (MQ=255)
  aaCAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCATCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACg                                                                                                                                      <  2:4765513/140‑1 (MQ=255)
       ggACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGAAGTTCCGATCCTCCAATTAACAGATTAAGAATCg                                                                                                                                 >  2:2011638/1‑140 (MQ=255)
        gACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCgg                                                                                                                                <  2:869860/140‑1 (MQ=255)
        gACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCgg                                                                                                                                <  2:32465/140‑1 (MQ=255)
         aCGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGTAGTGGCGCTCCTCCCATTAAAAGATTTCGAAGCGGt                                                                                                                               >  1:2091304/1‑140 (MQ=255)
         aCGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGt                                                                                                                               <  1:752283/140‑1 (MQ=255)
         aCGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTGCCTCTCCGCCAATTAAAAGATTTCGAAGCGGt                                                                                                                               >  2:1456564/1‑140 (MQ=255)
         aCGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTGCCTCGCCTCCAAATAACAGATGACGAAGCGGt                                                                                                                               >  1:4082515/1‑140 (MQ=255)
         aCGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGAGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGt                                                                                                                               <  1:1097598/140‑1 (MQ=255)
         aCGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTACTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCGCGCCTCCAATTAAAAGATTAACAAGCGGt                                                                                                                               >  1:961448/1‑140 (MQ=255)
            cATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTTCCTCTCCTCCAATTAACAGATTACGAAGCGGTAca                                                                                                                            >  2:2370959/1‑139 (MQ=255)
            cATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACt                                                                                                                            <  2:4259043/140‑1 (MQ=255)
            cATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGTAGTGGCTGTCCTGCAAATAAAAGATGACGAAGCGGTACt                                                                                                                            >  1:345930/1‑140 (MQ=255)
            cATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGGAGTGCCGCGCCTCCAATTAACAGATTACGAAGCGGGACt                                                                                                                            >  1:4940595/1‑140 (MQ=255)
             aTCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTc                                                                                                                           <  2:2908290/140‑1 (MQ=255)
             aTCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTc                                                                                                                           <  2:3650262/140‑1 (MQ=255)
             aTCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTc                                                                                                                           <  1:1642250/140‑1 (MQ=255)
                    gaaGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTcccc                                                                                                                    <  2:3397695/139‑1 (MQ=255)
                    caAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTcccc                                                                                                                    <  2:1524652/140‑1 (MQ=255)
                    caAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTcccc                                                                                                                    <  1:647705/140‑1 (MQ=255)
                       gTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAAATAACAGATGACGAAGCGGTAAACCTTCCCcaac                                                                                                                 >  1:3746372/1‑138 (MQ=255)
                            ggTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTg                                                                                                            <  1:359083/140‑1 (MQ=255)
                              ttGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTGCCTCTCCACCAATTTAAAGATGACGAAGCGGGACGCCTTCCCCATCATGTGCa                                                                                                          >  1:211291/1‑140 (MQ=255)
                                 gTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCTCCGCCAAATAACACATGACGAAGCGGTACTACTTCCCCAACAACTGCAGca                                                                                                       >  2:4637195/1‑139 (MQ=255)
                                 gTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCGCCTCCAATTAACAGATGACGGAGCGGTACTCCTTCCCCATCATCGTCAGCt                                                                                                       >  1:4828839/1‑140 (MQ=255)
                                 gTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCGCTCCGCCAATTAACAGATTAAGAATCGTGACAACTTCCCCAACAACAGCAACt                                                                                                       >  2:1173544/1‑140 (MQ=255)
                                       ataCATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACcagc                                                                                                 <  2:4547577/140‑1 (MQ=255)
                                            tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGTAGTGCCTCTCCTCCAATTAAAAGATGACGAAGCGGTACTCCTTACCCATCATCTGCAGCTACCAGCAGCtt                                                                                            >  1:2455663/1‑140 (MQ=255)
                                            tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGCAGTGCCTCTCCGCCAATTAACAGATGACGAATCGGTACTCCTTCCCCATCATATGCAGCGACCAGCAGCtt                                                                                            >  2:663331/1‑140 (MQ=255)
                                            tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGAAGTGGCGCTCCGCCAATTAAAAGATGACGAAGCGGTTCTCCTTCCCCCTCATCTGCAGCTACCAGCAGCtt                                                                                            >  1:1081304/1‑140 (MQ=255)
                                            tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGAAGTGCCTCTCCTCCAAATAAAACAAGACCTAGCGGGACTCCTTACCCCACATAGTCAGCTACCagcagcgt                                                                                            >  1:708770/1‑138 (MQ=255)
                                            tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTTCCTCTCCTCCAAATAAAAGAAGACGAAACGGTTCTACGTCACCAACATATGCAGATACCagcagcgt                                                                                            >  2:2749652/1‑138 (MQ=255)
                                             ttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACg                                                                                                                                      <  2:1926108/97‑1 (MQ=255)
                                              tgtgATGGCAGGTGCTGCTCTATCCGCGCCAAATACGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCCAATAACAGAGGAAGCAGCCGTACGACGTCCCCATCATGTGCCGCGACCACCACCTTCa                                                                                          >  2:4318697/1‑140 (MQ=255)
                                              tgtgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATTACCAAGCGGTACTCCTTACCCATCAACAGCAGCTAACCGCACCTTCa                                                                                          >  2:2507701/1‑140 (MQ=255)
                                              tgtgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCCCTCCTCCAAATAACAGATGACGAAGCGGTACTCCTTCCCCAACATAGGCAGCTACCCGCAGCTTCa                                                                                          >  2:1302284/1‑140 (MQ=255)
                                                tgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCTCCTCCAAGTAACAGATTACGAATCCGTACTCCATCCCCAAAATCAGCAGCTACCAGCACCTTaaaa                                                                                        >  2:3030657/1‑140 (MQ=255)
                                                tgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGGGCCTCTCCGGCAATTAAAACAAGAGGAAGGGGTAATCCTTCCCCATAAACTTCAGCTACCAGCCGCTTGaaa                                                                                        >  2:4126870/1‑140 (MQ=255)
                                                tgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTATTTGTAAGTGACAATCAGCCAAATAACAAATGACTAATAGTGAATCCTTCCCCATAAACTCCAGAAAACACCAGCTTCaaa                                                                                        >  2:1040466/1‑140 (MQ=255)
                                                  aTGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTGCCTCGCCGACAATTAACAAAAGAAGACGCGGTAATACTTCCCCAACAACTGCAGCTACCCGCCGCTTaaaaag                                                                                      >  1:1345814/1‑138 (MQ=255)
                                                      cAGGTGCTGCTCTCTCCGCGCAAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATgggcg                                                                                  <  1:3782726/140‑1 (MQ=255)
                                                      cAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATgggcg                                                                                  <  1:3003200/140‑1 (MQ=255)
                                                           gctgctCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGt                                                                             <  2:2411499/140‑1 (MQ=255)
                                                               ctctATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGTAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCg                                                                         <  1:3290691/140‑1 (MQ=255)
                                                               ctctATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCg                                                                         <  1:3067618/140‑1 (MQ=255)
                                                               ctctATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCg                                                                         <  2:739331/140‑1 (MQ=255)
                                                               ctctATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCg                                                                         <  1:4672632/140‑1 (MQ=255)
                                                               ctctATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCg                                                                         <  2:1356375/140‑1 (MQ=255)
                                                                   aTCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCgg                                                                     <  1:3373713/140‑1 (MQ=255)
                                                                    tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGTAGGTGCGCTCCTCCCAATTAAAGATTGCGAAGCGGTACGCCGTGCCCAACAACTTCCGCTCCCAGCACCATGAAAATTGGGGGCGTccccgcggg                                                                    >  1:1949147/1‑134 (MQ=255)
                                                                    tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGACGTGGCGCTCCTCCAATTAACACCTGACGAAGCGGTACGCCTTCCCCATCATCTGCACCGACCAGCAGCTGCAAAAGGGCGGGCGTCCCCTCGGt                                                                    >  1:259185/1‑140 (MQ=255)
                                                                    tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAATTTGCGCTCCTCCAATTTACAGATTAAGAAGGGGTAATCCTTCCCCATCATATGCAGCTAACAGCAGCTTAAAATTGGCGGGGGTCCCGTCGGt                                                                    >  1:4729236/1‑140 (MQ=255)
                                                                    tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTTCCTCTCCTCCAAATAACAGATTAAGAATCGGTACTACTTCCCCAACATCAGCAGCTACCAGCACCTTCAAATGGGCGGGCGTCCCGTCGGt                                                                    >  1:1239731/1‑140 (MQ=255)
                                                                    tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCGCCACCAAATAACAGATGACGAAGCGGTACTCCGTCCCCATCATCTGCAGCTACCCGCAGCTTCAAATGGGCGGGGGGCCCGTCggg                                                                    >  1:3627608/1‑139 (MQ=255)
                                                                    tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGTAGTGCCGCTCCGCCAATTAACACATTAAGAATCGGTACTACTTGCCCAACATAAGCAGCTACCAGCAGCATCAAAAGGGCGGGCGTccccgcggg                                                                    >  1:2159350/1‑134 (MQ=255)
                                                                    tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGGAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGt                                                                    <  1:2538956/140‑1 (MQ=255)
                                                                    tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGCAGTTCCTCTCCGACAAATAAAAGATGAAGAATCGGTACACCTTACCCATAAAATGCAGCTACCACCACCGTCAAAATGGCGGGCGTCCCCTCGGt                                                                    >  1:316655/1‑140 (MQ=255)
                                                                    tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGt                                                                    <  1:1885001/140‑1 (MQ=255)
                                                                    tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGt                                                                    <  2:2640020/140‑1 (MQ=255)
                                                                         gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCGTGTCCTCCAAATAACAAATTACGAAGAGGGACTCCTTACCCAAGATATGCACCGACCAACAGCTTGAAATTGGCGGGGGTACCGTCGGTTACGt                                                              >  2:684373/1‑140 (MQ=255)
                                                                         gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGAt                                                                                                                                          <  1:3525337/65‑1 (MQ=255)
                                                                         gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGt                                                              <  2:3591070/140‑1 (MQ=255)
                                                                         gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCGCCTCCAATTAAAAGATGACGAAGCGGTACTCCTTACCCATCCTCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTAAGt                                                              >  2:4429586/1‑140 (MQ=255)
                                                                         gcCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGTAGTGGCGCTCCGCCCATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCCGCTACCACCAGCTTCAAAAGGGCGGGCGTCCCCTCGGTTAAGt                                                              >  1:4566687/1‑140 (MQ=255)
                                                                         gcCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGTAGTTCCTCTCCTCCCATTTACAGAGTAAGAATCGGTACTCCTTTCACCTAAACTGCAGCTACCCCCAGATTGAAATCGGCGGGCGTGCCCTCGGTTAcct                                                              >  2:909594/1‑138 (MQ=255)
                                                                                aaGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACACATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCg                                                       <  2:4050283/140‑1 (MQ=255)
                                                                                aaGTGTATGAACTTGGTGACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACACATCACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTGCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCg                                                       <  2:3834352/140‑1 (MQ=255)
                                                                                   tgtATGAACTTGGTTACCGTCGTTTTTGGTAGTGGCTCTCCGCCAAATAACAGATGACGAAGCGGTACTCCTTCCCCATAATCATCAGCAACCAGCAGCGTCAAAAGGGCGGGCGGACCGTCGGTTACGTCCAATCGATg                                                    >  2:457670/1‑140 (MQ=255)
                                                                                   tgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCGACTCCCATTAACAGATTACGAAGCGGTACTCCTTCCCCAACATCTGCAGCTACCAGCAGCTTAAAATGGGCGGGCGTCCCGTCGGGTAAGGCAATTCGATg                                                    >  2:2150489/1‑140 (MQ=255)
                                                                                     tttGAACTTGGTTTCCGTCGTTTTTGGATGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCt                                                  <  2:2850392/138‑1 (MQ=255)
                                                                                     gaTGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCt                                                  <  1:2731100/139‑1 (MQ=255)
                                                                                          aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                             <  1:436186/140‑1 (MQ=255)
                                                                                          aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                             <  1:1801965/140‑1 (MQ=255)
                                                                                          aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa                                             <  1:2641248/140‑1 (MQ=255)
                                                                                           cTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGAt                                            <  1:839765/140‑1 (MQ=255)
                                                                                           cTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGAt                                            <  1:2664761/140‑1 (MQ=255)
                                                                                                ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt                                       <  1:99708/140‑1 (MQ=255)
                                                                                                ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt                                       <  1:291811/140‑1 (MQ=255)
                                                                                                ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt                                       <  1:2296675/140‑1 (MQ=255)
                                                                                                   ccGTCGTTTTTGGAAGTGCCTCTCCGCCCATTAACACATGACGAAGCGGTACACCTTCCCCAAAATCTGCAGCTACCAGCAGCTTCAAATTGGCGGGCGTCCCGGCGGTTACCGCCAATCGATGCTGTGGATAGTAtttg                                    >  2:1720702/1‑138 (MQ=255)
                                                                                                           tttGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGc                            <  2:535225/140‑1 (MQ=255)
                                                                                                             tGGAAGTGCCTCTCCTCCAATTCACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGAGAGTATTGGCATAAGGCCt                          <  1:410923/140‑1 (MQ=255)
                                                                                                             tGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCt                          <  2:2310097/140‑1 (MQ=255)
                                                                                                             tGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCt                          <  2:3535482/140‑1 (MQ=255)
                                                                                                             tGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCt                          <  1:2255955/140‑1 (MQ=255)
                                                                                                             tGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCt                          <  1:1497097/140‑1 (MQ=255)
                                                                                                             tGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCt                          <  1:1453106/140‑1 (MQ=255)
                                                                                                             tGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCt                          <  1:3851465/140‑1 (MQ=255)
                                                                                                               gAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCg                        <  1:1854096/140‑1 (MQ=255)
                                                                                                                   tGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCAt                    <  2:1858387/140‑1 (MQ=255)
                                                                                                                              ccAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGc          <  1:545887/140‑1 (MQ=255)
                                                                                                                              ccAATTAACAGATGACGAAGCGGTACTCCTTCCCCATAATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGc          <  2:4940595/140‑1 (MQ=255)
                                                                                                                               cAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGAt                                            >  1:4891682/1‑105 (MQ=255)
                                                                                                                                      cAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAg  >  2:3574770/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                          
TGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGCATAAGGCCTCGCCATCAGACACCGCCTCTTTAG  >  NZ_CP034943/1936483‑1936755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: