Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,491,058 T→G 6.0% E271D (GAA→GAC)  floA ← flotillin‑like protein FloA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,491,0580TG 6.0% 592.8 / 5.0 221E271D (GAA→GAC) floAflotillin‑like protein FloA
Reads supporting (aligned to +/- strand):  ref base T (138/62);  new base G (2/19);  total (140/81)
Fisher's exact test for biased strand distribution p-value = 1.23e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

CGAATGAATCACGCATTTCTGTGTCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCGATGTTTTTGCCAATGTC  >  NZ_CP034943/2490919‑2491195
                                                                                                                                           |                                                                                                                                         
cGAATGAATCACGCATTTCTGTGTCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCAtt                                                                                                                                           <  1:4540347/140‑1 (MQ=255)
cGAATGAATCACGCATTTCTGTGTCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCAtt                                                                                                                                           <  1:312786/140‑1 (MQ=255)
cGAATGAATCACGCATTTCTGTGTCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCAtt                                                                                                                                           <  1:280536/140‑1 (MQ=255)
          aCGCATTTCTGTGTCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTAcgc                                                                                                                                 >  2:1099851/1‑140 (MQ=255)
             cATTTCTGTGTCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCggg                                                                                                                              <  1:4333257/140‑1 (MQ=255)
             cATTTCTGTGTCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCggg                                                                                                                              <  1:3717292/140‑1 (MQ=255)
               tttCTGTGTCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGgcg                                                                                                                            <  1:3964616/140‑1 (MQ=255)
                 tCTGTGTCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGgcgcg                                                                                                                          <  2:2275112/140‑1 (MQ=255)
                 tCTGTGTCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAAGAGTCCCTTCCCGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGgcgcg                                                                                                                          <  2:3840954/140‑1 (MQ=255)
                        cAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACGTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTcc                                                                                                                   >  2:371231/1‑140 (MQ=255)
                          gCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCCCTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTg                                                                                                                 >  2:643819/1‑140 (MQ=255)
                          gCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTg                                                                                                                 >  1:1730372/1‑140 (MQ=255)
                          gCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTg                                                                                                                 >  1:1531214/1‑140 (MQ=255)
                          gCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTg                                                                                                                 >  2:576140/1‑140 (MQ=255)
                          gCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTg                                                                                                                 >  2:191131/1‑140 (MQ=255)
                          gCATCAATGTTCTTGATAATCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTACCTCTACGCGGGCGCGCATTTCCTg                                                                                                                 >  2:1123855/1‑140 (MQ=255)
                                 tGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGa                                                                                                          >  1:747947/1‑140 (MQ=255)
                                     cTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCACTTCCTGTTCTTGAGCGa                                                                                                      <  1:2051919/140‑1 (MQ=255)
                                     cTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATGTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGa                                                                                                      <  1:3329080/140‑1 (MQ=255)
                                     cTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCGACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGa                                                                                                      >  1:1401834/1‑140 (MQ=255)
                                      atGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCTCAAGCGGCCCTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATGTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGAc                                                                                                     <  2:1538887/139‑1 (MQ=255)
                                        gATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCTCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAg                                                                                                   >  1:3571519/1‑140 (MQ=255)
                                        gATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAg                                                                                                   >  1:2895560/1‑140 (MQ=255)
                                        gATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAg                                                                                                   >  1:4981942/1‑140 (MQ=255)
                                        gATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAg                                                                                                   >  1:3805644/1‑140 (MQ=255)
                                        gATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAg                                                                                                   <  2:3446662/140‑1 (MQ=255)
                                        gATATTAATGTAGTCCATGACGCCAATATTCCCTTCACGCAAACCTTATGACATCGCAAGCGGCACTTCAGCATAGGCTTCTACAAATTTATCGCGCATTTACTCTACTCTGGCGAGAATTTCCTGTTCTTGAGCTACAg                                                                                                   >  1:917106/1‑140 (MQ=255)
                                           atTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGa                                                                                                      <  2:3880557/134‑1 (MQ=255)
                                           atTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCa                                                                                                >  1:3013221/1‑140 (MQ=255)
                                           atTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCa                                                                                                >  1:603720/1‑140 (MQ=255)
                                           atTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCa                                                                                                >  1:1376638/1‑140 (MQ=255)
                                             tCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATc                                                                                              >  2:973687/1‑140 (MQ=255)
                                             tCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATc                                                                                              >  1:3982903/1‑140 (MQ=255)
                                             tCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCGTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCGTTTCCTCTACGCGGGCGCGCATTTCGTGTTCTTGAGCGACAGCCCTc                                                                                              >  1:2696925/1‑140 (MQ=255)
                                               aTGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGc                                                                                            >  2:3210288/1‑140 (MQ=255)
                                                tGTAGTCCATGACGCCAATCTTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCa                                                                                           <  2:3305761/140‑1 (MQ=255)
                                                tGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCa                                                                                           <  2:4474799/140‑1 (MQ=255)
                                                tGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATGTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCa                                                                                           <  2:4440097/140‑1 (MQ=255)
                                                  tAGTCCATGACGCCAATATTCCCGTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCAcg                                                                                         <  2:3976690/140‑1 (MQ=255)
                                                    gTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCAcgtc                                                                                       >  2:982919/1‑140 (MQ=255)
                                                     tCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCAcgtcg                                                                                      >  1:1627781/1‑140 (MQ=255)
                                                     tCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCAcgtcg                                                                                      >  1:4310990/1‑140 (MQ=255)
                                                     tCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATGTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCAcgtcg                                                                                      <  1:2483686/140‑1 (MQ=255)
                                                     tCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATGTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCAcgtcg                                                                                      >  1:2344433/1‑140 (MQ=255)
                                                     tCCATGACGCCAATATGCCCTACACGCACAGCTTCTGCAATCGCAAGCGGCACTTCCGCGTCGGCTTCTACCACTTTCGCGCGCCTTTCCGGTACGCGGGCGCGCAGGTCCTGTTCTTGAGCGACAGCCATCGCAcgtcg                                                                                      >  2:1739471/1‑140 (MQ=255)
                                                         tgccGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGATTCGCCCACTTTCGCGCGCATGTCCTCTACGCGGGGGCGGATTTCCTGTGCTTGAGCGACAGCCATCTCACGTCGttcg                                                                                  >  1:2116159/4‑139 (MQ=255)
                                                         tGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGttct                                                                                  >  1:3666437/1‑140 (MQ=255)
                                                          gACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGttctt                                                                                 >  2:3331364/1‑140 (MQ=255)
                                                          gACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGttctt                                                                                 >  1:4258437/1‑140 (MQ=255)
                                                          gACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGttctt                                                                                 >  1:2860818/1‑140 (MQ=255)
                                                          gACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATGTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGttctt                                                                                 <  1:2213661/140‑1 (MQ=255)
                                                          gACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCGTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGttctt                                                                                 >  1:1983832/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCggg                                                                                                                              >  2:2680329/1‑90 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCTGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                            >  1:2517627/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                            >  1:2596297/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                            >  2:276216/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                            >  2:2304473/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                            >  2:4567994/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                            >  2:4757552/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                            >  2:50947/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                            >  1:1662330/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                            >  1:1502495/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                            >  1:1087872/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                            >  2:1451133/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                            >  1:4645276/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTGCTAGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                            >  1:3394524/1‑140 (MQ=255)
                                                               cAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCAATTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCt                                                                            >  2:776967/1‑140 (MQ=255)
                                                                       ccTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTg                                                                    >  2:4892415/1‑140 (MQ=255)
                                                                         ttCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTgcg                                                                  >  1:4494224/1‑140 (MQ=255)
                                                                         ttCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTgcg                                                                  >  1:4750878/1‑140 (MQ=255)
                                                                          tCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCCTTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTgcgc                                                                 >  2:2922933/1‑140 (MQ=255)
                                                                          tCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTgcgc                                                                 >  2:1761618/1‑140 (MQ=255)
                                                                          tCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTgcgc                                                                 >  2:262026/1‑140 (MQ=255)
                                                                          tCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTgcgc                                                                 >  2:2901679/1‑140 (MQ=255)
                                                                          tCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTgcgc                                                                 >  2:2487143/1‑140 (MQ=255)
                                                                           cccGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGcgcg                                                                <  2:3142399/138‑1 (MQ=255)
                                                                           cACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGcgcg                                                                >  2:1557632/1‑140 (MQ=255)
                                                                           cACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGcgcg                                                                <  2:1431334/140‑1 (MQ=255)
                                                                           cACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGcgcg                                                                <  1:3102864/140‑1 (MQ=255)
                                                                                aaaGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCGCGACGCGGGCGCGCAGTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGtt                                                           <  1:2313894/140‑1 (MQ=255)
                                                                                aaaGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATGTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGtt                                                           <  1:2653771/140‑1 (MQ=255)
                                                                                     ttCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATGTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTAt                                                      <  2:1188755/140‑1 (MQ=255)
                                                                                     gtCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCAGGTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTAt                                                      <  1:2941927/139‑1 (MQ=255)
                                                                                       cTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCa                                                                                                                        <  2:4144297/72‑1 (MQ=255)
                                                                                       cTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCa                                                                                                                        >  1:4144297/1‑72 (MQ=255)
                                                                                        tGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCAGTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGttctt                                                                                 <  1:989008/110‑1 (MQ=255)
                                                                                              cGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCCTTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCgg                                             >  2:3667413/1‑140 (MQ=255)
                                                                                              cGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTAt                                                      <  1:22925/131‑1 (MQ=255)
                                                                                              cGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCgg                                             >  2:4718255/1‑140 (MQ=255)
                                                                                                        aCTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGt                                   <  2:4939152/140‑1 (MQ=255)
                                                                                                                        cTACCACGTTCGCGCGCATTTCCGCTCCGAGGGCGAGCATTTACTGTTCTTGAGCGACAGCCATCGCAAGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCGGATCGTTTTGTAAAATCGCTCCg                   <  1:3622466/140‑1 (MQ=255)
                                                                                                                              cTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCGATGttt             >  1:722084/1‑140 (MQ=255)
                                                                                                                               tttCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCCTCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAAGAGCTCCGATGtttt            >  2:2845130/1‑140 (MQ=255)
                                                                                                                               tttCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCGATGtttt            >  2:87509/1‑140 (MQ=255)
                                                                                                                                 tCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCGATGTTTTTg          >  1:3352862/1‑140 (MQ=255)
                                                                                                                                 tCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCGATGTTTTTg          >  2:2244355/1‑140 (MQ=255)
                                                                                                                                   gcgcgcATTTCCTCGACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCGATGTTTTTGcc        <  2:2547489/140‑1 (MQ=255)
                                                                                                                                    cgcgcATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCGATGTTTTTGCCa       <  2:4642757/140‑1 (MQ=255)
                                                                                                                                         aTTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCGATGTTTTTGCCAATGTc  <  2:206732/140‑1 (MQ=255)
                                                                                                                                         aTTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCGATGTTTTTGCCAATGTc  <  2:2346380/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
CGAATGAATCACGCATTTCTGTGTCAGCATCAATGTTCTTGATATTCATGTAGTCCATGACGCCAATATTCCCTTCACGCAAAGCTTCTGCCATCGCAAGCGGCACTTCCGCTTCGGCTTCTACCACTTTCGCGCGCATTTCCTCTACGCGGGCGCGCATTTCCTGTTCTTGAGCGACAGCCATCGCACGTCGTTCTTCCGCTTTTGCCTGCGCGATGTTTTTATCAGCCTCGGCCTGATCGGTTTGTAAAATCGCTCCGATGTTTTTGCCAATGTC  >  NZ_CP034943/2490919‑2491195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑