Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,979,834 C→T 10.8% noncoding (967/2928 nt) EO946_RS15430 ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,979,8340CT10.8% 1.6 / 4.6 38noncoding (967/2928 nt)EO946_RS1543023S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (2/30);  new base T (2/2);  total (5/33)
Fisher's exact test for biased strand distribution p-value = 5.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

CTGGTTGTCTAAGCAACTCCACATCCTTTTCCACTTAACGTATACTTTGGGACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCCTAGTCCAATCAGTGCTCTACCTCCAAGACTCTTACCTTGAGGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTATCTCCAGGTTCG  >  NZ_CP034943/2979700‑2979963
                                                                                                                                      |                                                                                                                                 
ctgggggTCTAAGCAACTCCACATCCCTGTCCACTTAACGTAGACTTTGGGACCTTAGCTGGCGGTCTGGGCTGTTTCCCGTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCg                                                                                                                              <  2:679259/134‑1 (MQ=1)
                              cctcTTCACGTATACTTTGGGACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAgg                                                                                                <  2:4326964/137‑1 (MQ=1)
                              ccccTTCACGTATACTTTGGGACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTCCGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAgg                                                                                                <  2:215906/137‑1 (MQ=0)
                              ccccTTAACGTATACTTTGGGACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAgg                                                                                                <  2:2110908/137‑1 (MQ=1)
                              ccccTTAACGTATACTTTGGGACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAgg                                                                                                <  2:3681707/137‑1 (MQ=1)
                              ccccTTAACGTATACTTTGGGACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCAATCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAgg                                                                                                <  2:4483850/137‑1 (MQ=1)
                              ccccTTAACGTATACTGTGGGACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAgg                                                                                                <  2:1628045/137‑1 (MQ=1)
                                  ttcaCGTATACTTTGGGACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGTATTCGGAGGTTGACTGAAGTCGGTCCACCGGTAGGGGcc                                                                                            >  2:320588/4‑140 (MQ=1)
                                   taccGTATACTTTGGGACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGccc                                                                                           <  2:2250360/137‑1 (MQ=1)
                                   taccGTATACTTTGGGACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGccc                                                                                           <  2:2063881/137‑1 (MQ=1)
                                   taccGTATACTTTGGGACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGccc                                                                                           <  2:1958679/137‑1 (MQ=1)
                                   taccGTATACTTTGGGACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGccc                                                                                           <  2:1085855/137‑1 (MQ=1)
                                   taccGTATACTTTGGGACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGccc                                                                                           <  1:3804164/137‑1 (MQ=1)
                                   taccGTATACTTTGGGACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGccc                                                                                           <  2:816252/137‑1 (MQ=1)
                                   taccGTATACTTTGGGACCGTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGccc                                                                                           <  1:4648826/137‑1 (MQ=1)
                                   taaccgataCTTTGGGACCTTAGCTGGCGGTCTGGGCTGTATCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCACGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGccc                                                                                           <  2:640832/134‑1 (MQ=0)
                                             ttggggACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCCTAGTCCAAt                                                                                 <  2:2390907/137‑1 (MQ=1)
                                             ttggggACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCCTAGTCCAAt                                                                                 <  2:2214885/137‑1 (MQ=1)
                                               tgtgACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATATTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCCTAGTCCAATCa                                                                               <  2:754194/137‑1 (MQ=1)
                                               tgcgACCTTAGCTGGCGGTCAGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCTCAGTCTGACTCCCAAGGATAAGTCAATGGAATTCGGAGTTTGACTGAAGTCGGTAACCCGGTAGGGGCCCCTAGTCCAATCa                                                                               >  2:3601715/4‑140 (MQ=1)
                                                     ctgaGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCCTAGTCCAATCAGTGctc                                                                         <  2:2359322/137‑1 (MQ=1)
                                                     ctgaGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCCTAGTCCAATCAGTGctc                                                                         <  2:3490152/137‑1 (MQ=1)
                                                      ttcgCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGTATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCCTAGTCCAATCAGTGctct                                                                        <  2:2968983/137‑1 (MQ=1)
                                                      ttcgCTGGCGGTCTGGGCTGTTTCCCTTTAGACTACGGATCTTATCACTAGCAGTCTGACTCCCAAGGATAAGTCATTGGACTTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCCTAGTCCAATCAGTGctct                                                                        <  1:4246071/137‑1 (MQ=1)
                                                      ttcgCTGGCGGTCTGGGCTGGTTCCCGTTAGACTACGGATCTTATCACTCGCAGTCGGACGCCCAAGGATCAGTCATTGGCATTCGGAGTTTGACTGAATGCGGTAACCCGGTAGGGGCCCCTAGTCCAATCTCTGctct                                                                        <  2:513046/137‑1 (MQ=1)
                                                      gtcgcgggCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTGATTGGCATTCGGAGTTTGACTGACTTCGGTAACCCGGTAGGGGCCCGTAGTCCAATCAGTGctct                                                                        <  2:1322530/134‑1 (MQ=1)
                                                      gccgcaggCGGTCTGGGCTGTGTCCCTTTCGACTACGGCTGTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCCTAGTCCAATCAGTGctct                                                                        <  2:3355638/134‑1 (MQ=1)
                                                            gtctgtatGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCCTAGTCCAATCAGTGCTCTACCTcc                                                                  <  2:3276503/133‑1 (MQ=0)
                                                            gtcgttCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCATAGTCCAATCAGTGCTCTACCTcc                                                                  <  1:3378820/135‑1 (MQ=0)
                                                            ggccggcTGGGCTGGTTCCCTGTCGACTACGGATCTGATCACACGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCCTAGTCCAATCAGTGCTCTACCTcc                                                                  <  2:4500519/134‑1 (MQ=1)
                                                                         gtgtCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCCTAGTCCAATCAGTGCTCTACCTCCAAGACTCTTACCt                                                     <  1:3553017/137‑1 (MQ=1)
                                                                               ctgtCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCCTAGTCCAATCAGTGCTCTACCTCCAAGACTCTTACCTTGAGGc                                               <  2:2633563/137‑1 (MQ=1)
                                                                                ttgcGACTACGGATCGTATCACTCGCAGTCTGACTCCCAAGCATAAGTCATTGGCCTTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCCTAGTCCAATCCCTGCTCTACCTCCACGACTCTTACCTGGAGGcg                                              <  2:1342499/137‑2 (MQ=1)
                                                                                     cctccGGTTCTGATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCCTAGTCCAATCAGTGCTCTACCTCCAAGACTCTTACCTTGAGGCTAGccc                                         <  2:370472/136‑1 (MQ=0)
                                                                                                      tcacAGTCTGACTCCCAAGGATAAGTCATTGGTATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCCTAGTCCAATCAGTGCTCTACCTCCAAGACTCTTACCTTGAGGCTAGCCCTAAAGCTATTTCGgaga                        >  1:2740186/4‑140 (MQ=1)
                                                                                                                aatcacAAGGATAAGTCATTTGCATTCGTATTTTGACTTAATTCGGTAACCCGGTAGGGGAACCTAGTCCAATCAGTGATCTACCTCCAATAATATTACATAGAGGCTAGCCCTAAAGCTATTTCGGAGAGAAACAGCTa              >  2:4746867/6‑140 (MQ=1)
                                                                                                                    cccaGGCTAAGTCCTTGGCATTCGGCGTTTGTCTGAATTCGGTAAGCCGGTAGGGGCGCCTAGTCCAATCAGTGCTCTACCTCCAAGTCTCTTACCTTGTGGCTAGCCCTAAAGCTATTTCGGAGTGAGCCCGCTTtctc          >  2:1856046/4‑140 (MQ=1)
                                                                                                                            aactCATTGGTATTCTGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCATAGTCCAATCAGTGCTCGACCTCCAAGACTCTTACCTTGAGGCTAGCCCGAAAGCTATTTCTGAGAGAACCAGCTATCTCCAGGTTCg  <  2:1290385/137‑1 (MQ=1)
                                                                                                                                      |                                                                                                                                 
CTGGTTGTCTAAGCAACTCCACATCCTTTTCCACTTAACGTATACTTTGGGACCTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGCCCCTAGTCCAATCAGTGCTCTACCTCCAAGACTCTTACCTTGAGGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTATCTCCAGGTTCG  >  NZ_CP034943/2979700‑2979963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑