Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,217,687 T→C 7.2% K166K (AAA→AAG sigO ← RNA polymerase sigma factor SigO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,217,6870TC 7.2% 623.3 / 27.3 195K166K (AAA→AAGsigORNA polymerase sigma factor SigO
Reads supporting (aligned to +/- strand):  ref base T (60/121);  new base C (7/7);  total (67/128)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

Only 100 of 213 total aligned reads displayed.

GTATTGCAGAGTTTTTTTCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAG  >  NZ_CP034943/3217548‑3217824
                                                                                                                                               |                                                                                                                                         
gTATTGCAGAGTTTTTTTCTGATCATCGGTGAAACGCATTCAATCAGAGGTTCAATGTCAAATGTATCGTCTGGTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTGCGTGCCCCCTTGTTTTGCGCCGGTAGCTGt                                                                                                                                           >  1:4677181/1‑140 (MQ=255)
 tttagCAGACTTTTTGTGTGATCCTCGGTGAACAGCATTCGATCCGTTGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGTCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGtt                                                                                                                                          <  1:3741282/136‑1 (MQ=255)
 tagagCAGAGTTTTTTTCTGATCATCGTTTAAAAGCATTCTATCAGATGTTCAATGTCCAATTTATCGTCTTTTTGGTTA‑‑‑TTTCGGTCAAACGGGGCGTTAACAGCTTAATTTCCCCCTTTTTTGGCGCCGCTAGCTGtt                                                                                                                                          <  1:1327794/136‑1 (MQ=255)
 tATTGCAGAGTTTTTTTCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGtt                                                                                                                                          <  1:3155846/140‑1 (MQ=255)
 tATTGCAGAGTTTTTTTCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGtt                                                                                                                                          <  1:1802251/140‑1 (MQ=255)
  aTTGCAGAGTTTTTTTCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGttt                                                                                                                                         >  2:1945329/1‑140 (MQ=255)
  aTTGCAGAGTTTTTTTCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGttt                                                                                                                                         >  2:1808041/1‑140 (MQ=255)
      cAGAGTTTTTTTCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTg                                                                                                                                     <  1:4399295/140‑1 (MQ=255)
      cAGAGTTTTTTTCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTg                                                                                                                                     <  1:7939/140‑1 (MQ=255)
       agagTTTTTTTCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGa                                                                                                                                    >  2:1724359/1‑140 (MQ=255)
        gagTTTTTTTCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGat                                                                                                                                   <  2:1647760/140‑1 (MQ=255)
         agTTTTTTTCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGata                                                                                                                                  >  2:4563439/1‑140 (MQ=255)
         agTTTTTTTCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGata                                                                                                                                  >  2:1604727/1‑140 (MQ=255)
         agTTTTTTTCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGata                                                                                                                                  >  2:2107942/1‑140 (MQ=255)
         agTTTTTTTCTGATCATCGGCGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGGGTTTTTGGGTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACGTTCCCCCGTTTTTTGGGCCGCTAGCTGTTTTCTGtta                                                                                                                                  >  1:4316686/1‑140 (MQ=255)
           tttttttCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATAtt                                                                                                                                >  1:4098882/1‑140 (MQ=255)
           tttttttCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATAtt                                                                                                                                >  1:4787643/1‑140 (MQ=255)
           tttttttCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCGTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATAtt                                                                                                                                >  1:1878565/1‑140 (MQ=255)
                 tCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAg                                                                                                                          <  2:2209462/140‑1 (MQ=255)
                 tCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAg                                                                                                                          <  2:4136885/140‑1 (MQ=255)
                   tGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGcc                                                                                                                        <  2:4892824/140‑1 (MQ=255)
                     atcatcGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCCAACTGGCCGTTCACAGCGTAGTTTGGCCCTTGTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCGAGCCCt                                                                                                                      >  1:2982538/1‑140 (MQ=255)
                     atcatcGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGc                                                                                                                         <  2:3791534/137‑1 (MQ=255)
                     atcatcGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCt                                                                                                                      >  2:1256266/1‑140 (MQ=255)
                           ggTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTa                                                                                                                <  2:1087390/140‑1 (MQ=255)
                                       tCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCGCCTTTTTTTGCGCCGCTAGCTGTTGTCTGATATTCTCAAGCCCTTTTGTATGAATGTTGgag                                                                                                    >  2:2370124/1‑140 (MQ=255)
                                       tCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGgag                                                                                                    <  1:2940256/140‑1 (MQ=255)
                                       tCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGgag                                                                                                    >  2:774013/1‑140 (MQ=255)
                                       tCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGgag                                                                                                    <  2:1758340/140‑1 (MQ=255)
                                         aaTCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGtt                                                                                                                                          >  2:1414867/1‑100 (MQ=255)
                                         aaTCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGtt                                                                                                                                          <  1:1414867/100‑1 (MQ=255)
                                           tCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGt                                                                                                <  2:2652863/140‑1 (MQ=255)
                                           tCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGt                                                                                                <  2:2152199/140‑1 (MQ=255)
                                           tCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTATTTATGAATGTTGGAGATGt                                                                                                <  2:1639492/140‑1 (MQ=255)
                                           ccAGATCGTCAATGTCAAATTTATCGACATTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGGTCACAGCTTACTTTCCCCCATTTTGGGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGt                                                                                                <  1:1731964/139‑1 (MQ=255)
                                                tGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGc                                                                                           <  1:610827/140‑1 (MQ=255)
                                                tGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGc                                                                                           <  1:1074809/140‑1 (MQ=255)
                                                 gTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGcc                                                                                          >  2:64623/1‑140 (MQ=255)
                                                 gTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGcc                                                                                          >  2:1121471/1‑140 (MQ=255)
                                                  ctCAATGTCATAAGTCTCGTCTTTTTGTTTCTTTTTTTGTTCAAACTGGCCGTCCACAGCTTACTTTTCCCCCTTTTTTTGCGCCGCTAACTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGtttt                                                                                             >  2:208527/2‑140 (MQ=255)
                                                  ctCAATGTCATAAGTCTCGTCTTTTTGTTTCTTTTTTTGTTCAAACTGGCCGTCCACAGCTTACTTTTCCCCCTTTTTTTGCGCCGCTAACTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGtttt                                                                                             >  2:2557222/2‑140 (MQ=255)
                                                   tCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCgg                                                                                        <  1:4852965/140‑1 (MQ=255)
                                                   tCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCgg                                                                                        <  2:3011704/140‑1 (MQ=255)
                                                   tCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCgg                                                                                        <  1:2085339/140‑1 (MQ=255)
                                                   tCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCACAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCgg                                                                                        <  1:381033/140‑1 (MQ=255)
                                                    cAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCggg                                                                                       >  2:3732280/1‑140 (MQ=255)
                                                     cacgTCAACTTTATCGTCGTTTTGGCTC‑‑‑TTGTGTTCAAACGGGCCGTTCACAGCGTACTTTCCCCCCTGTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                      <  2:2898497/137‑1 (MQ=255)
                                                     aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                      <  1:3250789/140‑1 (MQ=255)
                                                     aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                      <  1:1604727/140‑1 (MQ=255)
                                                     aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                      <  1:165536/140‑1 (MQ=255)
                                                     aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                      <  1:2062167/140‑1 (MQ=255)
                                                     aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                      <  1:2426796/140‑1 (MQ=255)
                                                     aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                      <  2:4242837/140‑1 (MQ=255)
                                                     aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                      <  2:3424926/140‑1 (MQ=255)
                                                     aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                      <  1:4486543/140‑1 (MQ=255)
                                                     aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTGTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                      <  2:3864225/140‑1 (MQ=255)
                                                     aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTGTCCCCCTTTGTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                      <  2:4499168/140‑1 (MQ=255)
                                                            aaTTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCt                                                                               >  2:4168473/1‑140 (MQ=255)
                                                            aaTTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCt                                                                               >  2:1615279/1‑140 (MQ=255)
                                                            aaTTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTATTTATGAATGTTGTAGATGTTTTGACGGGACTCTCCt                                                                               >  2:2497522/1‑140 (MQ=255)
                                                            aaTTTATCGACTTTTTTGTTC‑‑‑TATTGTTAAAACTGGCCTTTCACATCTTACGATACCCCTTTTTTTTCTCCGCTAGCTGTTTTCTGATATAATCAAGACCTTTTTTATGAATATTGGAGATCTTTAGCAGCTACTCACCt                                                                               >  2:4957565/1‑140 (MQ=255)
                                                            aaTTTATCGACTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCGTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCACGCCCTTTTTTATGAATGTTGGAGATGCTTTGCCGGGACTCTCCt                                                                               <  1:1862184/140‑1 (MQ=255)
                                                            aaTTGAGCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCt                                                                               <  1:4183483/140‑1 (MQ=255)
                                                                 aTCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGa                                                                          <  1:620352/140‑1 (MQ=255)
                                                                 aTCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTGTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGa                                                                          <  1:2284883/140‑1 (MQ=255)
                                                                      atttttGGTTC‑‑‑TTTTGTTCAAACTGGCCTTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCgg                                                                     <  2:1177682/139‑1 (MQ=255)
                                                                        ttttGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCa                                                                   <  2:1295626/140‑1 (MQ=255)
                                                                        ttttGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCa                                                                   <  1:4563439/140‑1 (MQ=255)
                                                                        ttttGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCCTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCa                                                                   <  2:3442369/140‑1 (MQ=255)
                                                                               tC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCtt                                                            >  2:4715563/1‑140 (MQ=255)
                                                                               tC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCGTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCtt                                                            >  1:3854229/1‑140 (MQ=255)
                                                                                c‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTg                                                           <  1:1121471/140‑1 (MQ=255)
                                                                                c‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTg                                                           <  2:557379/140‑1 (MQ=255)
                                                                                c‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTg                                                           <  2:44754/140‑1 (MQ=255)
                                                                                        gTTCAAACTGGCCGTTCACAGCTTCCTGTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCg                                                      <  2:321592/140‑1 (MQ=255)
                                                                                        gTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCg                                                      <  2:810761/140‑1 (MQ=255)
                                                                                                tGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCa                                              <  1:546255/140‑1 (MQ=255)
                                                                                                  gCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                      <  2:837806/98‑1 (MQ=255)
                                                                                                  gCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                      >  1:837806/1‑98 (MQ=255)
                                                                                                  gCCGTTCACAGCTTACGTTCCCCCTTGTTTTGCGCCGCGAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCAACGTGGCACCATg                                            <  2:4399702/140‑1 (MQ=255)
                                                                                                            gCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACt                                  >  2:1578509/1‑140 (MQ=255)
                                                                                                            gCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACt                                  >  2:2020296/1‑140 (MQ=255)
                                                                                                                aCTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCg                              >  2:1210890/1‑140 (MQ=255)
                                                                                                                      cccccTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTg                                                           >  2:1056439/1‑106 (MQ=255)
                                                                                                                       ccccTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCa                        <  2:3854229/140‑1 (MQ=255)
                                                                                                                       ccccTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCa                        <  1:3922512/140‑1 (MQ=255)
                                                                                                                       ccccTTTTTGTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGTAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCa                        <  1:4021253/140‑1 (MQ=255)
                                                                                                                       ccccTTTATTTGCGCCGCTAGCTGTTTTCTGATATTCTCCCGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCa                        <  1:1898621/140‑1 (MQ=255)
                                                                                                                        cccTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCAc                       >  2:3635798/1‑140 (MQ=255)
                                                                                                                         ccTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGGCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACg                      <  1:975840/140‑1 (MQ=255)
                                                                                                                           tttttttGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCATAGTGAATCTGCAAGCTCTTGCATCTTGGCACCATGAAGATAGACTTTCGTCAGCACGCt                    >  1:2039617/1‑140 (MQ=255)
                                                                                                                                ttGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCtttttt               <  1:736230/140‑1 (MQ=255)
                                                                                                                                 tGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCa                                                                   >  1:2717133/1‑87 (MQ=255)
                                                                                                                                 tGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCa                                                                   <  2:2717133/87‑1 (MQ=255)
                                                                                                                                           gCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTgtcagtc    <  2:1227900/140‑1 (MQ=255)
                                                                                                                                           acTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGTTTTGTCGGGACTCCCCTAATGAATCTGCAATCTCCTGCATCGTGGCACCGTGAAGATAGACTTTCGTCAGCACACTTTTTTGTTTgtcagtc    <  1:2626778/139‑1 (MQ=255)
                                                                                                                                           acTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGTTTTGTCGGGACTCCCCTAATGAATCTGCAATCTCCTGCATCGTGGCACCGTGAAGATAGACTTTCGTCAGCACACTTTTTTGTTTgtcagtc    <  1:2557222/139‑1 (MQ=255)
                                                                                                                                           acTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGTTTTGTCGGGACTCCCCTAATGAATCTGCAATCTCCTGCATCGTGGCACCGTGAAGATAGACTTTCGTCAGCACACTTTTTTGTTTgtcagtc    <  1:3734206/139‑1 (MQ=255)
                                                                                                                                           acTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGTTTTGTCGGGACTCCCCTAATGAATCTGCAATCTCCTGCATCGTGGCACCGTGAAGATAGACTTTCGTCAGCACACTTTTTTGTTTgtcagtc    <  1:1253867/139‑1 (MQ=255)
                                                                                                                                           acTGCTTTCTGATAGTCTCCAGCCCCGTTTTATGAATGTTGGAGATGTTTTGTCGGGACTCCCCTAATGAATCTGCAATCTCCTGCATCGTGGCACCGTGAAGATAGACTTTCGTCAGCACACTTTTTGGTTTgtcagtc    <  1:1014108/139‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                         
GTATTGCAGAGTTTTTTTCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAG  >  NZ_CP034943/3217548‑3217824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: