Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,217,702 A→C 7.3% L161L (CTT→CTG sigO ← RNA polymerase sigma factor SigO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,217,7020AC 7.3% 642.3 / 21.1 216L161L (CTT→CTGsigORNA polymerase sigma factor SigO
Reads supporting (aligned to +/- strand):  ref base A (59/138);  new base C (7/11);  total (67/149)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

TCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCGCTTGGT  >  NZ_CP034943/3217565‑3217841
                                                                                                                                             |                                                                                                                                           
tCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAg                                                                                                                                           <  2:2209462/140‑1 (MQ=255)
  tGATCATCGTTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGcc                                                                                                                                         <  2:1062787/140‑1 (MQ=255)
    atcatcGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCt                                                                                                                                       >  2:1256266/1‑140 (MQ=255)
    atcatcGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACGTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCt                                                                                                                                       >  1:1836352/1‑140 (MQ=255)
            tGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATg                                                                                                                               <  2:1709823/140‑1 (MQ=255)
              aaaaGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGaa                                                                                                                             <  2:1088720/140‑1 (MQ=255)
                 aGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGt                                                                                                                          <  2:4348374/140‑1 (MQ=255)
                 aGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGt                                                                                                                          <  2:394977/140‑1 (MQ=255)
                   cATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTg                                                                                                                        >  2:4223592/1‑140 (MQ=255)
                          tCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCACACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGt                                                                                                                 <  2:1754692/140‑1 (MQ=255)
                          tCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGt                                                                                                                 <  2:2152199/140‑1 (MQ=255)
                               tGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGc                                                                                                            <  1:610827/140‑1 (MQ=255)
                               tGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGc                                                                                                            <  1:1074809/140‑1 (MQ=255)
                                gTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGcc                                                                                                           >  2:1121471/1‑140 (MQ=255)
                                gTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGcc                                                                                                           >  2:64623/1‑140 (MQ=255)
                                 ctCAATGTCATAAGTCTCGTCTTTTTGTTTCTTTTTTTGTTCAAACTGGCCGTCCACAGCTTACTTTTCCCCCTTTTTTTGCGCCGCTAACTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGtttt                                                                                                              >  2:208527/2‑140 (MQ=255)
                                 ctCAATGTCATAAGTCTCGTCTTTTTGTTTCTTTTTTTGTTCAAACTGGCCGTCCACAGCTTACTTTTCCCCCTTTTTTTGCGCCGCTAACTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGtttt                                                                                                              >  2:2557222/2‑140 (MQ=255)
                                 ctCAATGTCATAAGTCTCGTCTTTTTGTTTCTTTTTTTGTTCAAACTGGCCGTCCACAGCTTACTTTTCCCCCTTTTTTTGCGCCGCTAACTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGtttt                                                                                                              >  2:1630596/2‑140 (MQ=255)
                                 ctCAATGTCATAAGTCTCGTCTTTTTGTTTCTTTTTTTGTTCAAACTGGCCGTCCACAGCTTACTTTTCCCCCTTTTTTTGCGCCGCTAACTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGtttt                                                                                                              >  2:2626778/2‑140 (MQ=255)
                                 ctCAATGTCATAAGTCTCGTCTTTTTGTTTCTTTTTTTGTTCAAACTGGCCGTCCACAGCTTACTTTTCCCCCTTTTTTTGCGCCGCTAACTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTGGGAGATGtttt                                                                                                              >  2:1014108/2‑140 (MQ=255)
                                  tCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCgg                                                                                                         <  1:4852965/140‑1 (MQ=255)
                                  tCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCgg                                                                                                         <  1:2085339/140‑1 (MQ=255)
                                  tCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCgg                                                                                                         <  2:2666841/140‑1 (MQ=255)
                                  tCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCgg                                                                                                         <  1:4094431/140‑1 (MQ=255)
                                  tCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCgg                                                                                                         <  2:3011704/140‑1 (MQ=255)
                                  tCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCACAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCgg                                                                                                         <  1:381033/140‑1 (MQ=255)
                                   cAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCggg                                                                                                        >  2:3732280/1‑140 (MQ=255)
                                    cacgTCAACTTTATCGTCGTTTTGGCTC‑‑‑TTGTGTTCAAACGGGCCGTTCACAGCGTACTTTCCCCCCTGTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                       <  2:2898497/137‑1 (MQ=255)
                                    aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCACACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                       <  2:2849047/140‑1 (MQ=255)
                                    aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGt                                                                                                                 >  1:2955880/1‑130 (MQ=255)
                                    aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                       <  1:1724359/140‑1 (MQ=255)
                                    aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                       <  1:1604096/140‑1 (MQ=255)
                                    aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                       <  2:4156595/140‑1 (MQ=255)
                                    aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                       <  1:482037/140‑1 (MQ=255)
                                    aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                       <  2:3424926/140‑1 (MQ=255)
                                    aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                       <  1:4486543/140‑1 (MQ=255)
                                    aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                       <  1:2426796/140‑1 (MQ=255)
                                    aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                       <  1:165536/140‑1 (MQ=255)
                                    aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                       <  1:3732280/140‑1 (MQ=255)
                                    aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                       <  2:2461051/140‑1 (MQ=255)
                                    aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                       <  2:757244/140‑1 (MQ=255)
                                    aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                       <  1:2062167/140‑1 (MQ=255)
                                    aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTGTCCCCCTTTGTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                       <  2:4499168/140‑1 (MQ=255)
                                    aaTGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTACAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                       <  1:3216717/140‑1 (MQ=255)
                                           aaTTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCt                                                                                                >  2:783515/1‑140 (MQ=255)
                                           aaTTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCt                                                                                                >  2:3823874/1‑140 (MQ=255)
                                           aaTTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCt                                                                                                >  2:4954738/1‑140 (MQ=255)
                                           aaTTGAGCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCt                                                                                                <  1:4183483/140‑1 (MQ=255)
                                                aTCGTCTTTTTGGTTC‑‑‑TTTTGTTCAACCTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCGAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGa                                                                                           <  1:2888146/140‑1 (MQ=255)
                                                aTCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGa                                                                                           <  1:620352/140‑1 (MQ=255)
                                                aTCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGa                                                                                           <  1:3851735/140‑1 (MQ=255)
                                                aTCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGa                                                                                           <  1:3738380/140‑1 (MQ=255)
                                                     atttttGGTTC‑‑‑TTTTGTTCAAACTGGCCTTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCgg                                                                                      <  2:1177682/139‑1 (MQ=255)
                                                       ttttGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCa                                                                                    <  1:1373374/140‑1 (MQ=255)
                                                       ttttGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCa                                                                                    <  1:4563439/140‑1 (MQ=255)
                                                       ttttGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCa                                                                                    <  2:1609239/140‑1 (MQ=255)
                                                       ttttGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCa                                                                                    <  2:1287807/140‑1 (MQ=255)
                                                              tC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGGTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCtt                                                                             >  1:3855382/1‑140 (MQ=255)
                                                              tC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCtt                                                                             >  1:2548105/1‑140 (MQ=255)
                                                              tC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCtt                                                                             >  2:4715563/1‑140 (MQ=255)
                                                               c‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTg                                                                            <  2:557379/140‑1 (MQ=255)
                                                               c‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTg                                                                            <  1:1121471/140‑1 (MQ=255)
                                                                       gTTCAAACTGGCCGTTCACAGCTTCCTGTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCg                                                                       <  2:321592/140‑1 (MQ=255)
                                                                                 gCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGa                                                                                                       <  2:837806/98‑1 (MQ=255)
                                                                                 gCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATg                                                             <  2:1976153/140‑1 (MQ=255)
                                                                                 gCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATg                                                             <  2:1381512/140‑1 (MQ=255)
                                                                                      tCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGAt                                                        >  2:1764587/1‑140 (MQ=255)
                                                                                           gCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACt                                                   >  2:4433924/1‑140 (MQ=255)
                                                                                               aCTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCg                                               >  2:1210890/1‑140 (MQ=255)
                                                                                               aCTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCg                                               >  2:3103604/1‑140 (MQ=255)
                                                                                                     cccccTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGACTCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGc                                          >  1:1863838/1‑140 (MQ=255)
                                                                                                     cccccTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGc                                          <  1:3027089/140‑1 (MQ=255)
                                                                                                      ccccTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCa                                         <  1:2248877/140‑1 (MQ=255)
                                                                                                      ccccTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCa                                         <  2:4846633/140‑1 (MQ=255)
                                                                                                      ccccTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCa                                         <  2:3855382/140‑1 (MQ=255)
                                                                                                      ccccTTTTTGTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGTAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCa                                         <  1:4021253/140‑1 (MQ=255)
                                                                                                          tttttttGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCt                                     >  1:835140/1‑140 (MQ=255)
                                                                                                               ttGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCtttttt                                <  1:736230/140‑1 (MQ=255)
                                                                                                               ttGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCtttttt                                <  1:2320892/140‑1 (MQ=255)
                                                                                                                tGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCa                                                                                    >  1:2717133/1‑87 (MQ=255)
                                                                                                                          gCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTgtcagtc                     <  2:1227900/140‑1 (MQ=255)
                                                                                                                          acTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGTTTTGTCGGGACTCCCCTAATGAATCTGCAATCTCCTGCATCGTGGCACCGTGAAGATAGACTTTCGTCAGCACACTTTTTTGTTTgtcagtc                     <  1:2626778/139‑1 (MQ=255)
                                                                                                                          acTGCTTTCTGATATTCTCCAGCCCCTTTTTATGAATGTTGGAGATGTTTTGTCGGGACTCCCCTAATGAATCTGCAATCTCCTGCATCGTGGCACCGTGAAGATAGACTTTCGTCAGCACACTTTTTTGTTTgtcagtc                     <  1:1253867/139‑1 (MQ=255)
                                                                                                                          acTGCTTTCTGATAGTCTCCAGCCCCGTTTTATGAATGTTGGAGATGTTTTGTCGGGACTCCCCTAATGAATCTGCAATCTCCTGCATCGTGGCACCGTGAAGATAGACTTTCGTCAGCACACTTTTTGGTTTgtcagtc                     <  1:1014108/139‑1 (MQ=255)
                                                                                                                                 tCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCAt                                                              >  1:510187/1‑92 (MQ=255)
                                                                                                                                     atatTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAAt          >  1:3546876/1‑140 (MQ=255)
                                                                                                                                       atTCTCCAGCCCTTTTTTATGAACGCTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGACAGTCAGCTTCTGAATCg        <  1:849281/140‑1 (MQ=255)
                                                                                                                                       atTCTCAAGCCCTTTTTTCTGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCg        <  1:1615279/140‑1 (MQ=255)
                                                                                                                                       atTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCg        <  1:1764587/140‑1 (MQ=255)
                                                                                                                                       atTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCg        <  1:4248096/140‑1 (MQ=255)
                                                                                                                                       atTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCg        <  1:2020296/140‑1 (MQ=255)
                                                                                                                                       atTCTCAAGACCTTTTTTATGTATGTTTGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGGACTCTTTTTTGTTTTTCAGTCAGCTTCTGAATCg        <  1:4957565/140‑1 (MQ=255)
                                                                                                                                       agtcgcAAGCCCAGTTTTATGAACGCTGGAGATGTTGTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTACGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCg        <  1:4336435/135‑1 (MQ=255)
                                                                                                                                        ttCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATg                                                             >  2:509434/1‑86 (MQ=255)
                                                                                                                                        ttCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATg                                                             <  1:509434/86‑1 (MQ=255)
                                                                                                                                        ttCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATg                                                             >  2:3603323/1‑86 (MQ=255)
                                                                                                                                        ttCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCGc       >  1:3350997/1‑140 (MQ=255)
                                                                                                                                        ttCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCGc       >  1:2283682/1‑140 (MQ=255)
                                                                                                                                        ttCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATAGc       >  2:3758841/1‑140 (MQ=255)
                                                                                                                                          ctcAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCGCtt     >  2:4963402/1‑140 (MQ=255)
                                                                                                                                             |                                                                                                                                           
TCTGATCATCGGTGAAAAGCATTCAATCAGATGTTCAATGTCAAATTTATCGTCTTTTTGGTTC‑‑‑TTTTGTTCAAACTGGCCGTTCACAGCTTACTTTCCCCCTTTTTTTGCGCCGCTAGCTGTTTTCTGATATTCTCAAGCCCTTTTTTATGAATGTTGGAGATGTTTTGCCGGGACTCTCCTAGTGAATCGGCAATCTCTTGCATCGTGGCACCATGAAGATAGACTTTCGTCAGCACGCTTTTTTGTTTGTCAGTCAGCTTCTGAATCGCTTGGT  >  NZ_CP034943/3217565‑3217841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: