BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,247,065	C→T	100%	intergenic (‑11/+110)	bdbD ← / ← cadA	disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,247,065	0	C	T	99.1%	977.6 / ‑2.6	280	intergenic (‑11/+110)	bdbD/cadA	disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase
Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (167/108); total (169/111)
Fisher's exact test for biased strand distribution p-value = 5.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 280 total aligned reads displayed.

TCCGGAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTCATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATG  >  NZ_CP034943/3246929‑3247196

tCCGGAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATc                                                                                                                                  >  2:846458/1‑140 (MQ=255)

tCCGGAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATc                                                                                                                                  >  1:2701421/1‑140 (MQ=255)

tCCGGAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATc                                                                                                                                  >  1:3677273/1‑140 (MQ=255)

tCCGGAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACCGCTGTTTCTTTTTCACTCCGACACCTTATc                                                                                                                                  >  1:1317240/1‑140 (MQ=255)

                      tggtCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGGAAGGATGACCGCGAATTTCGCACAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAaagaag                                                                                                            <  1:2578126/137‑1 (MQ=255)

                      tGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAaataag                                                                                                            >  1:1723652/1‑140 (MQ=255)

                      tGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAaagaag                                                                                                            <  1:4751057/140‑1 (MQ=255)

                      tGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAaagaag                                                                                                            >  1:3153512/1‑140 (MQ=255)

                        ttCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACAGCTTATCGTTTTAAAATTAAAAAAAGAATCg                                                                                                          >  1:4173147/1‑140 (MQ=255)

                        ttCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAATCg                                                                                                          >  1:3001713/1‑140 (MQ=255)

                        ttCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCg                                                                                                          >  1:4087778/1‑140 (MQ=255)

                        ttCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCg                                                                                                          >  1:393872/1‑140 (MQ=255)

                        ttCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCg                                                                                                          >  2:659014/1‑140 (MQ=255)

                        ttCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCg                                                                                                          >  2:4791340/1‑140 (MQ=255)

                        ttCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCg                                                                                                          >  1:321442/1‑140 (MQ=255)

                        ttCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCg                                                                                                          >  1:2887473/1‑140 (MQ=255)

                        ttCCGTTTTGTAATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTAGCGTTTTAAAATTAAAAAaagaacag                                                                                                          >  2:769253/1‑137 (MQ=255)

                          ccGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACCAACACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTTGTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGa                                                                                                        <  1:4247702/140‑1 (MQ=255)

                          ccGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGa                                                                                                        <  2:1759965/140‑1 (MQ=255)

                          ccGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGa                                                                                                        <  1:5824/140‑1 (MQ=255)

                           ctttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAACGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                       <  2:3277060/138‑1 (MQ=255)

                           cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCTTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                       <  2:261522/140‑1 (MQ=255)

                           cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                       <  2:1900362/140‑1 (MQ=255)

                           cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                       <  1:2332633/140‑1 (MQ=255)

                           cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                       <  2:1779527/140‑1 (MQ=255)

                           agTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                       <  2:3957191/139‑1 (MQ=255)

                             ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAaagaag                                                                                                            <  1:4977220/133‑1 (MQ=255)

                             ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                     >  1:2031336/1‑140 (MQ=255)

                             ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                     >  1:1015996/1‑140 (MQ=255)

                             ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                     >  1:1620244/1‑140 (MQ=255)

                             ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACACCGACACCTTATAGATTTAAAATTAAAAAAAGAAGATGATCg                                                                                                     >  1:1839812/1‑140 (MQ=255)

                             ttttGTTATTAAAGATGACAATGCCTCCTAGTAAGACCACAACCACAACCGTAAGGATGACCGCTAATTTCGCAGAAGACTGATGTTTCTTTTTCACTCCGACACCTGATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                     >  2:1036756/1‑140 (MQ=255)

                                 gTTATTAAAGATGACAATGTCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTAGCAGAATACTTCTGTTTCTTTTTCACTCCTACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAAt                                                                                                 <  2:4038071/140‑1 (MQ=255)

                                 gTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAAt                                                                                                 <  1:1607760/140‑1 (MQ=255)

                                 gTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAAt                                                                                                 <  2:1989348/140‑1 (MQ=255)

                                  ttattaAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATa                                                                                                >  2:1289582/1‑140 (MQ=255)

                                  ttattaAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATa                                                                                                >  2:3836075/1‑140 (MQ=255)

                                      taAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGAcc                                                                                            <  1:3371351/140‑1 (MQ=255)

                                      taAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGAcc                                                                                            <  1:3258488/140‑1 (MQ=255)

                                       aaaGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGAccc                                                                                           >  2:1534553/1‑140 (MQ=255)

                                       aaaGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGAccc                                                                                           >  2:1442227/1‑140 (MQ=255)

                                       aaaGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGAccc                                                                                           >  2:2987797/1‑140 (MQ=255)

                                       aaaGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGAccc                                                                                           >  2:3892067/1‑140 (MQ=255)

                                             gACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCActtct                                                                                     <  2:134145/140‑1 (MQ=255)

                                               cAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCttt                                                                                   >  1:4357591/1‑140 (MQ=255)

                                                  tGGCTGCTCGTACGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTGCTTTAtt                                                                                >  2:4542376/1‑140 (MQ=255)

                                                      tgctAGTAAGAAAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAATACTGCTGTTTCTTTTTCACTCGGACACCTTATCTTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTaa                                                                            <  1:642701/140‑1 (MQ=255)

                                                       gctAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAac                                                                           <  1:4933942/140‑1 (MQ=255)

                                                              aGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagacaa                                                                    >  1:661608/1‑140 (MQ=255)

                                                              aGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagacaa                                                                    >  1:708153/1‑140 (MQ=255)

                                                              aGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagacaa                                                                    <  2:3868617/140‑1 (MQ=255)

                                                              aGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagacaa                                                                    >  2:4693945/1‑140 (MQ=255)

                                                              aGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagacaa                                                                    >  1:1656585/1‑140 (MQ=255)

                                                              aGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagacaa                                                                    >  1:1739906/1‑140 (MQ=255)

                                                              aGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagacaa                                                                    >  2:4813343/1‑140 (MQ=255)

                                                              aGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCAGACACATTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagacaa                                                                    >  2:3112529/1‑140 (MQ=255)

                                                              aGACAACAACCACAACCGTAAGGATGACCGCGAATGTAGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagacaa                                                                    <  1:4806046/140‑1 (MQ=255)

                                                                 caacaaCCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTg                                                                 <  2:4919986/140‑1 (MQ=255)

                                                                  aacaacCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGc                                                                >  1:4300781/1‑140 (MQ=255)

                                                                   acaaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGcc                                                               <  2:1250065/140‑1 (MQ=255)

                                                                   acaaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGcc                                                               <  1:136156/140‑1 (MQ=255)

                                                                   acaaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGcc                                                               <  1:4556587/140‑1 (MQ=255)

                                                                    caaccacaaccGTAAGGATGACCGCGAATTTCGCATAAGACTGCTGTTTCTTTTTCACTCCGACACATTATCGTTTTAAAATTAAAAAAATAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAATACAAGTTCCt                                                              >  2:1013810/1‑140 (MQ=255)

                                                                    caaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCt                                                              >  2:1625306/1‑140 (MQ=255)

                                                                    caaccacaaccCTAAGGATGACCGCGAATTTCGCAGAAGACTTCAGTTTCTTATTCACAACGAAACCTTATCGTTTTAAAATTAAAAAAAAAAGAGTATCTCAATATACCAAAGTCTTAATATTAACAATACAAGTTCCt                                                              >  2:2189736/1‑140 (MQ=255)

                                                                          caaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGa                                                        >  1:2862209/1‑140 (MQ=255)

                                                                           aaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGaa                                                       <  1:4511592/140‑1 (MQ=255)

                                                                            accGTAAGGATGACCGCGAATTTAGCAGAATACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGaaa                                                      >  2:1950820/1‑140 (MQ=255)

                                                                                    gATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaa                                              <  2:4972112/140‑1 (MQ=255)

                                                                                    gATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaa                                              <  2:3677280/140‑1 (MQ=255)

                                                                                    gATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaa                                              <  2:3031871/140‑1 (MQ=255)

                                                                                    gATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaa                                              <  2:2701421/140‑1 (MQ=255)

                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTATAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATGTTCACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                          >  2:4332739/1‑140 (MQ=255)

                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATATACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                          >  2:4267412/1‑140 (MQ=255)

                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGcc                                                               <  2:117355/119‑1 (MQ=255)

                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAATAAAACCCCGaaaaaa                                          >  2:596052/1‑140 (MQ=255)

                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                          >  2:363794/1‑140 (MQ=255)

                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                          >  1:1945432/1‑140 (MQ=255)

                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                          >  1:4858286/1‑140 (MQ=255)

                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                          >  1:3430015/1‑140 (MQ=255)

                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                          >  2:704429/1‑140 (MQ=255)

                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                          >  2:4040177/1‑140 (MQ=255)

                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                          >  2:4320278/1‑140 (MQ=255)

                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                          >  2:4138275/1‑140 (MQ=255)

                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATAGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGcc                                                               >  1:117355/1‑119 (MQ=255)

                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTAACCTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACGTCTTTATTTTAACAAGACAAGTGCCTATAAGAACACCCCGaaaaaa                                          >  2:528032/1‑140 (MQ=255)

                                                                                              aaTTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAAtt                                    >  2:2070219/1‑140 (MQ=255)

                                                                                               aTTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATtc                                   >  1:4581138/1‑140 (MQ=255)

                                                                                                gttCGCAGAAGACTGCTGGTTCTTTTTCACTCCGACACCTCATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATtct                                  <  1:3758080/139‑1 (MQ=255)

                                                                                                    gCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTcc                              <  2:4858286/140‑1 (MQ=255)

                                                                                                      agaagaCTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGa                            <  1:371022/140‑1 (MQ=255)

                                                                                                             tgctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTa                     >  2:339381/1‑140 (MQ=255)

                                                                                                             tgctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTa                     >  2:3213866/1‑140 (MQ=255)

                                                                                                             tgctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAAGTTa                     >  2:1315667/1‑140 (MQ=255)

                                                                                                                  tttCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGt                >  1:842330/1‑140 (MQ=255)

                                                                                                                       ttttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTg           >  1:4333366/1‑140 (MQ=255)

                                                                                                                       ttttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTg           >  2:2338917/1‑140 (MQ=255)

                                                                                                                       ttttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTATCGCCGCCAAGTTATCCGattttt           >  1:3094663/1‑139 (MQ=255)

                                                                                                                             cTCCGACACCTTATCTTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAACACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTACGACAATTTATCCGTTTTTGAGACga     <  2:410662/140‑2 (MQ=255)

                                                                                                                             cTCCGACACCTTATCGTTTTAAAATGAAAAAAATAAGCGGATCGCAATACACCCCATTCTTTATTTTAACAAGACAAGGGCCTATAAGAAAACCCCGAAACAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGc     <  2:2897661/140‑1 (MQ=255)

TCCGGAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTCATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATG  >  NZ_CP034943/3246929‑3247196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`