BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,285,647	C→A	34.0%	V252L (GTA→TTA)	EO946_RS16995 ←	IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,285,647	0	C	A	34.0%	896.4 / inf	765	V252L (GTA→TTA)	EO946_RS16995	IS3 family transposase
Reads supporting (aligned to +/- strand): ref base C (299/205); new base A (183/77); total (483/282)
Fisher's exact test for biased strand distribution p-value = 2.67e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

Only 100 of 790 total aligned reads displayed.

TAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCATTC  >  NZ_CP034943/3285508‑3285786

tAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAc                                                                                                                                             <  1:1267730/140‑1 (MQ=40)

tAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAc                                                                                                                                             <  1:3453439/140‑1 (MQ=40)

tAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAc                                                                                                                                             <  1:2305789/140‑1 (MQ=40)

         taCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAatat                                                                                                                                    >  2:1383532/1‑140 (MQ=255)

              aGAACCTTGGTCACTCTGTAACACGCAGTTCTCAGTCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGGCTGTGTCCTGCTTATCCACAATCGTAAATGCAATCACTTCTCCCTTGTGTACATCTAATATACTgg                                                                                                                               >  2:4404382/1‑140 (MQ=14)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                                                                                                               >  1:298355/1‑140 (MQ=255)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                                                                                                               >  1:4073665/1‑140 (MQ=255)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                                                                                                               >  2:3536058/1‑140 (MQ=255)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTgg                                                                                                                               >  1:890666/1‑140 (MQ=255)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGGCCTGCTTATCCGCAATCGTAAAAGCACTCACTTCTCCATTGTATACATCTAATATACTgg                                                                                                                               >  1:1761944/1‑140 (MQ=255)

               gAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTa                                                                                                                                        <  2:1259387/130‑1 (MQ=255)

               gAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTa                                                                                                                                        >  1:212499/1‑130 (MQ=255)

               gAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGa                                                                                                                              >  1:3874851/1‑140 (MQ=255)

               gAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGa                                                                                                                              >  2:165704/1‑140 (MQ=255)

               gAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGa                                                                                                                              >  2:2566458/1‑140 (MQ=255)

               gAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGa                                                                                                                              >  1:802215/1‑140 (MQ=255)

               gAAACTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCAGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGAAATCACTTCTCAATTGTATACAACTAATATACTCGa                                                                                                                              >  1:941065/1‑140 (MQ=14)

                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGaa                                                                                                                             >  2:3154084/1‑140 (MQ=255)

                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGaa                                                                                                                             <  2:2049473/140‑1 (MQ=255)

                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                                                                                                             <  2:630523/140‑1 (MQ=255)

                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                                                                                                             >  2:2354929/1‑140 (MQ=255)

                   cTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAgg                                                                                                                          >  2:3739621/1‑140 (MQ=255)

                   cTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAgg                                                                                                                          <  2:4217588/140‑1 (MQ=255)

                   cTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAgg                                                                                                                          <  1:3308306/140‑1 (MQ=255)

                   cTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAgg                                                                                                                          <  1:1489347/140‑1 (MQ=255)

                     tGGTCACTATGTCACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAATAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTa                                                                                                                        >  2:389125/1‑140 (MQ=255)

                     tGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTa                                                                                                                        >  1:1851151/1‑140 (MQ=255)

                     tGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTa                                                                                                                        >  1:1800220/1‑140 (MQ=255)

                     tGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTa                                                                                                                        >  1:4355612/1‑140 (MQ=255)

                      ggTTACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTAc                                                                                                                       >  1:143981/1‑140 (MQ=255)

                      ggTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAATTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTACATTGTATAAATCTAATATAATGTAAAGGTAc                                                                                                                       >  2:2526741/1‑140 (MQ=11)

                      ggTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTAc                                                                                                                       >  2:1741076/1‑140 (MQ=255)

                            tATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGt                                                                                                                 >  2:2231274/1‑140 (MQ=255)

                            tATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGATGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACATCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGt                                                                                                                 >  2:1837228/1‑140 (MQ=11)

                            tATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGt                                                                                                                 >  1:1082940/1‑140 (MQ=255)

                              tGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGttt                                                                                                               <  2:4295642/140‑1 (MQ=255)

                                   cacGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTc                                                                                                          >  2:3070684/1‑140 (MQ=255)

                                      gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCTATCGTAAATGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                       >  2:1568414/1‑140 (MQ=11)

                                      gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                       >  2:1168286/1‑140 (MQ=255)

                                      gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                       >  2:4593718/1‑140 (MQ=255)

                                      gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                       >  2:1764379/1‑140 (MQ=255)

                                       cAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATa                                                                                                      <  1:490471/140‑1 (MQ=255)

                                        aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAATAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACACTTGTTTCTGTCCATaa                                                                                                     >  1:1558665/1‑140 (MQ=255)

                                        aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                                                                                                     >  1:2598760/1‑140 (MQ=255)

                                        aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                                                                                                     >  2:2421871/1‑140 (MQ=255)

                                        aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                                                                                                     >  2:2927680/1‑140 (MQ=255)

                                          ttCTCAGGCAGTGTTGGTAGCTGATGAAGTTTGTCTAAGACAAAGTCTGTGTCCGGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAgg                                                                                                   >  1:1122502/1‑140 (MQ=11)

                                          ttCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAgg                                                                                                   >  1:3212294/1‑140 (MQ=255)

                                            ctcAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCa                                                                                                 <  2:4761757/140‑1 (MQ=255)

                                            ctcAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCa                                                                                                 <  1:4038741/140‑1 (MQ=255)

                                               aGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGACAGGTACAATTGTTTCTGTCCATAAGGCAAat                                                                                              >  2:539014/1‑140 (MQ=11)

                                               aGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAat                                                                                              >  1:756418/1‑140 (MQ=255)

                                               aGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAat                                                                                              >  1:3178883/1‑140 (MQ=255)

                                                    gtgtTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACCATTGTTTCTGTCCATAAGGCAAATAtgtg                                                                                         >  2:3400320/1‑140 (MQ=255)

                                                               tgatgaAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTAc                                                                              >  2:78078/1‑140 (MQ=12)

                                                               tgatgaAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTAc                                                                              >  1:3548568/1‑140 (MQ=12)

                                                                 atgaaGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAat                                                                                              <  1:3808902/119‑1 (MQ=12)

                                                                    aaGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAAtt                                                                         >  1:3635479/1‑140 (MQ=12)

                                                                        aTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTc                                                                     >  1:342327/1‑140 (MQ=40)

                                                                         tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGTTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCa                                                                    >  2:1910616/1‑140 (MQ=255)

                                                                         tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGt                                                                                 >  1:672170/1‑127 (MQ=25)

                                                                         tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCa                                                                    >  2:4495776/1‑140 (MQ=21)

                                                                         tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCa                                                                    >  2:3619848/1‑140 (MQ=21)

                                                                         tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCa                                                                    >  2:2771444/1‑140 (MQ=21)

                                                                           tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                  >  1:414597/1‑140 (MQ=40)

                                                                           tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                  >  1:4266841/1‑140 (MQ=40)

                                                                             tAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAagag                                                                <  2:2638197/140‑1 (MQ=21)

                                                                                   ataGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATTAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGAt                                                          <  1:50166/138‑1 (MQ=255)

                                                                                   aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGAt                                                          <  1:167546/140‑1 (MQ=40)

                                                                                   aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGAt                                                          <  2:3948229/140‑1 (MQ=40)

                                                                                   aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGAt                                                          <  2:1097204/140‑1 (MQ=21)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGATGGCGATCa                                                        >  2:3211773/1‑140 (MQ=255)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                        >  1:3739101/1‑140 (MQ=40)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                        >  2:2724432/1‑140 (MQ=40)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAAGTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGGTACTAGTTTTTCTAGAGGGCGATCa                                                        >  2:4927119/1‑140 (MQ=255)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                        >  1:2779397/1‑140 (MQ=21)

                                                                                      gTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAg                                                       <  2:1550861/140‑1 (MQ=40)

                                                                                           tgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGa                                                  >  2:4044726/1‑140 (MQ=40)

                                                                                                 gCTTATCCCCAATCGTAAAAGCAATCACCTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                            >  1:2245489/1‑140 (MQ=255)

                                                                                                 gCTTATCCACAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGCTACAATTGTTTCTGTCCATAAGGCAAATATGGGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGTAAGTTc                                            <  1:4660768/140‑1 (MQ=11)

                                                                                                   ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATGAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                          <  2:875331/140‑1 (MQ=255)

                                                                                                     aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGAt                                        >  1:452450/1‑140 (MQ=18)

                                                                                                      tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  1:1331680/140‑1 (MQ=17)

                                                                                                      tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  1:392130/140‑1 (MQ=17)

                                                                                                      tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTATCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                       <  1:1189728/140‑1 (MQ=12)

                                                                                                       ccccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATcc                                      <  1:2400802/140‑1 (MQ=37)

                                                                                                       ccccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATcc                                      >  2:3328/1‑140 (MQ=14)

                                                                                                            aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAACGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatag                                 >  1:3485438/1‑139 (MQ=21)

                                                                                                            aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                 >  1:2685432/1‑140 (MQ=34)

                                                                                                               gTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATAtatt                              >  2:3449602/1‑140 (MQ=32)

                                                                                                                       aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                      <  1:2513961/140‑1 (MQ=32)

                                                                                                                       aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                      <  1:2919635/140‑1 (MQ=32)

                                                                                                                       aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                      <  2:1146129/140‑1 (MQ=32)

                                                                                                                       aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                      <  1:4592242/140‑1 (MQ=32)

                                                                                                                       aaTCACTTCGCCAGTCTAGACATCTAAGATACTGGAAAGGTACCATTGTTTCTGTCCATAAGGCAAATAGGTGATGTCCGTTACTAGTTGTTCAAGAGGGCGATCAGACTGACAGTTCCGATCCAATATATTATCGACCa                      <  2:2833113/140‑1 (MQ=11)

                                                                                                                            cTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCa                 <  1:1017159/140‑1 (MQ=32)

                                                                                                                                 ccATTGTATACATCTAATATTCTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATgg            <  1:601329/140‑1 (MQ=17)

                                                                                                                                      gTATACATCTAATATACTGTAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGcc       <  2:4853340/140‑1 (MQ=17)

                                                                                                                                       tataCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGccc      >  1:3039183/1‑140 (MQ=32)

                                                                                                                                           cATCTAATATACTGGAAAGGGACAATTGTTTCTGTCCATACGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCCttc  >  1:1953947/1‑140 (MQ=2)

TAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCATTC  >  NZ_CP034943/3285508‑3285786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`