Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,319,761 T→G 7.2% pseudogene (544/1292 nt) EO946_RS17170 ← arabinogalactan endo‑beta‑1,4‑galactanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,319,7610TG 7.2% 743.2 / 7.6 296pseudogene (544/1292 nt)EO946_RS17170arabinogalactan endo‑beta‑1,4‑galactanase
Reads supporting (aligned to +/- strand):  ref base T (119/137);  new base G (32/2);  total (157/139)
Fisher's exact test for biased strand distribution p-value = 4.73e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 305 total aligned reads displayed.

AATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGCCAG  >  NZ_CP034943/3319625‑3319900
                                                                                                                                        |                                                                                                                                            
aaTACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTGCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGa                                                                                                                                          <  2:940383/140‑1 (MQ=255)
 aTACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCACTGTCGACGCCTTCCTTGat                                                                                                                                         >  2:915864/1‑140 (MQ=255)
 aTACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCGTCCTTGag                                                                                                                                         >  2:4372897/1‑139 (MQ=255)
  tACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGatc                                                                                                                                        <  1:2436523/140‑1 (MQ=255)
    ccTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGatcat                                                                                                                                      <  1:3436349/140‑1 (MQ=255)
    ccTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGatcat                                                                                                                                      <  1:253918/140‑1 (MQ=255)
        ggTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCttt                                                                                                                                  >  2:4179157/1‑140 (MQ=255)
        ggTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTACGACCTGAACCATGCCAATGTCGACGCCATACATGATCAGCttt                                                                                                                                  >  2:4836643/1‑140 (MQ=255)
        ggTCTGTCTCCCTGACCGCGCGGCGTCCTTCATTAAATAATTGGCTCATTTACGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTGTCCTTTACGACCCGAACCATTCCAAAGACCACGCCATCCCGGatcatagtt                                                                                                                                  >  1:2970205/1‑136 (MQ=255)
          tctgtctCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCGTGCGTGTTCATCTTTTg                                                                                                                                >  1:2176171/1‑140 (MQ=255)
              tctcCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCGTCCTTGAGCATCTTTTGCAgg                                                                                                                            >  2:1053751/1‑140 (MQ=255)
              tctcCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCAGTTCCGACCTGAACCCTGCCCAGGTCGACGCCTTCCGTGATCATCGTTTGCAgg                                                                                                                            >  1:4205738/1‑140 (MQ=255)
                tcCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCt                                                                                                                          <  2:2398962/140‑1 (MQ=255)
                tcCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCt                                                                                                                          <  2:4982092/140‑1 (MQ=255)
                  ccTGACCGCTCGGCTTCCTTCATTATATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCttt                                                                                                                        <  1:4409171/140‑1 (MQ=255)
                  ccTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCttt                                                                                                                        >  2:273428/1‑140 (MQ=255)
                  ccTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCttt                                                                                                                        <  2:1969235/140‑1 (MQ=255)
                  ccTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTGTCATGTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCCTGTTTTGCAGGCttt                                                                                                                        >  2:297354/1‑140 (MQ=255)
                    tGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGt                                                                                                                      <  1:1874890/140‑1 (MQ=255)
                    tGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGt                                                                                                                      <  2:2235845/140‑1 (MQ=255)
                      aCCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTGTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGttt                                                                                                                    >  2:3073262/1‑140 (MQ=255)
                        cgcgcgGGTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAAACAAGCCAAAGTCGACGCCTGACTTGATCATATTTTGGAGGCTTTGtttag                                                                                                                  >  1:3995732/1‑138 (MQ=255)
                        cgcgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTGATTTCCGACCTGAACCATGACAAATTCCACGCCTTCCTTGATGATATTTTGCAGGCTTTTTTTCg                                                                                                                  >  1:1320822/1‑140 (MQ=255)
                        cgcgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCAAGCCAAAGTCGACGCCTTCCTTGCTCAACTTTTTCAGGCTTTGTTTCg                                                                                                                  >  1:637094/1‑140 (MQ=255)
                        cgcgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGAACAGAACCATGCCAATTTAGAAGCCTTCCGTGATAATCTTTTTCAGTCTTTTTTTCg                                                                                                                  >  1:1002028/1‑140 (MQ=255)
                          ggcgGCTTCCTTCATTAAATACTTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAACTGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCGTGATCATCTTTGGCAGCCTTTGTTTCGta                                                                                                                <  1:1036955/139‑1 (MQ=255)
                          cgcgGCTTCCTTGATTATATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACGTGAACCATTCAAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                                                                <  1:3934318/140‑1 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAACTGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                                                                <  2:1876213/140‑1 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTGATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTTTTTCGta                                                                                                                >  2:1474100/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTGATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCGTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                                                                >  2:3464691/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                                                                >  1:3597621/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                                                                <  1:2960437/140‑1 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                                                                >  2:3609142/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                                                                <  2:4112675/140‑1 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                                                                >  2:443695/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                                                                >  2:604673/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                                                                >  2:475473/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                                                                <  1:4179157/140‑1 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTGTGCAGGCTTTGTTTCGta                                                                                                                <  2:1814244/140‑1 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAAACATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCGTTGTTTCGta                                                                                                                >  2:252757/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAAACATGCCAATGTAGACGCCTTCCTTGATCAACTTTTGCAGGCTTTGTTTCGta                                                                                                                >  2:2845304/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCCACCTGAAACATGCCAATGTCGACGCCATCCGTGATCATCGTTTTCAGGCTTTGTGTCGta                                                                                                                >  1:967523/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATATCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCAGATTTTGCAGGCTGTGTTTCGta                                                                                                                >  2:4581372/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTAATTTCCGACCTGAAACATGCCAATGTCGACGCCTTCCTTGATCATCTTTTTCAGGCTTTGTTTCGta                                                                                                                >  2:4453887/1‑140 (MQ=255)
                          cgcgGCTTCCTTCATTAAATAATTGGCTCACTGTCGCCCAATCGGTTGCACCTGCAAATGCCCCTGTCGTGTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGta                                                                                                                <  2:4886847/140‑1 (MQ=255)
                          cgcgGCTGCCTGCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCTTTTCCATTCCCACCTTAAACAAGCCCAAGTAGAAGCCCTCCGTGATAATAATTTTCAGGCGTTGTTTCTta                                                                                                                >  1:2605205/1‑140 (MQ=255)
                           gcgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGGCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGtat                                                                                                               <  2:1268118/140‑1 (MQ=255)
                            cgGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGtata                                                                                                              <  2:1372974/140‑1 (MQ=255)
                            ccgCTGCCTTCACTAAATAATTGGCTCCTTGTCGCCCAATCGGTTTCACCTGCAAAGGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGtata                                                                                                              <  2:3778581/138‑1 (MQ=255)
                                     tcATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTGTCAATTGCGACCAGAACCCAGCCAATTTACAAGCCTTCCGGGAAACAAATTTTCAGGGTTTGTTTGGTATAAACAAagag                                                                                                     >  1:1927347/1‑140 (MQ=255)
                                                ttGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCACTGTCGACGCCTTCCGTGATCATCTTTTGCAGGCGTTGTTTCGGATACTCATAGAGCTTTGtttttt                                                                                          >  2:4316350/1‑140 (MQ=255)
                                                ttGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAAGGTCGACGCCGTCCTTGAGGATCTTTTGCAGGGTTTGTGTCGTATACTCATAGAGCTTTGtttttt                                                                                          >  2:2566431/1‑140 (MQ=255)
                                                 tGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTGATTTACGACCTGAACCATGCCAATGTCGACGCCTTGCGGGATCCTGGTTTGCAGGCTGTGTGTGGTTTACTCAAAGAGCTTTGttttttt                                                                                         >  1:3647393/1‑140 (MQ=255)
                                                      cATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCtt                                                                                    <  1:475473/140‑1 (MQ=255)
                                                      cATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCtt                                                                                    <  1:1501966/140‑1 (MQ=255)
                                                             gCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCt                                                                             <  1:2658205/140‑1 (MQ=255)
                                                                 aaTCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGc                                                                         <  1:3116149/140‑1 (MQ=255)
                                                                 aaTAGGGTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTGGTTTTTTTAGCTTCAAAGCTGAGc                                                                         <  1:2122963/140‑1 (MQ=255)
                                                                  aTCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGcc                                                                        <  2:845290/140‑2 (MQ=255)
                                                                  aTCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCt                                                                        <  2:321736/140‑1 (MQ=255)
                                                                  aTCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCAGAACCATGCCACTGTCGACGCCTTCCTTGATCATCATTTGCAGGCATTGTTTCGTATACTCATAGAGCATTGTTTTTTGAGCTTCAAAGCTGAGcg                                                                        <  2:4281957/140‑2 (MQ=255)
                                                                   tCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCtt                                                                       <  1:2740089/140‑1 (MQ=255)
                                                                   tCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCGTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCtt                                                                       <  1:2327617/140‑1 (MQ=255)
                                                                   tCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCGTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCGTTGTTTTTTTAGCTTCAAAGCTGAGCtt                                                                       >  2:1563945/1‑140 (MQ=255)
                                                                       tttCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGcc                                                                   <  1:1739851/140‑1 (MQ=255)
                                                                       tttCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTGAGCTTCAAAGCTGAGCTTCGcc                                                                   <  1:4651175/140‑1 (MQ=255)
                                                                       tttCACCTGCAAATGCCCCTGTCGTTTAATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCCTAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGcc                                                                   <  2:2605205/140‑1 (MQ=255)
                                                                         tCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCa                                                                 <  2:4717585/140‑1 (MQ=255)
                                                                                           gTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTg                                               <  1:3535374/140‑1 (MQ=255)
                                                                                                 tCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCt                                                                                                                          >  2:3308161/1‑59 (MQ=255)
                                                                                                 tCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCt                                                                                                                          <  1:3308161/59‑1 (MQ=255)
                                                                                                   aTTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCAGCTTTTGCAGGGTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCGTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTGCTGTTTCGCAg                                       >  2:1946930/1‑140 (MQ=255)
                                                                                                      tCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGAt                                    <  1:60419/140‑1 (MQ=255)
                                                                                                                 ccATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTAGACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGaaa                         <  1:4836643/140‑1 (MQ=255)
                                                                                                                  cATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAAt                        <  1:3836829/140‑1 (MQ=255)
                                                                                                                  cATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAAt                        <  1:161519/140‑1 (MQ=255)
                                                                                                                  cATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAAt                        <  1:4975995/140‑1 (MQ=255)
                                                                                                                  cATGCCAATGTCGACGCCGTCCTTGATCATCTTTTGCAGGCTTTGTTTCGGATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAAt                        <  1:1835772/140‑1 (MQ=255)
                                                                                                                    tGCCAATGTCGACGCCTTCGTTGATCAGCTTTTGCAGGCGTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCTGGATCGGCCCAGAAATCa                      >  1:205175/1‑140 (MQ=255)
                                                                                                                    tGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCa                      >  2:702156/1‑140 (MQ=255)
                                                                                                                    tGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCa                      >  2:1013677/1‑140 (MQ=255)
                                                                                                                    tGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCa                      >  2:4335646/1‑140 (MQ=255)
                                                                                                                    tGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCa                      >  2:1681112/1‑140 (MQ=255)
                                                                                                                     gCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAg                     >  1:1662059/1‑140 (MQ=255)
                                                                                                                     gCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCGGGTTCGCAGGATCGGCCCAGAAATCAg                     <  2:216878/140‑1 (MQ=255)
                                                                                                                      ccAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCATTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGa                    <  2:227842/140‑1 (MQ=255)
                                                                                                                         aTGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGaata                 >  2:1154080/1‑140 (MQ=255)
                                                                                                                         aTGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTGTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTCCCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGaata                 >  1:115953/1‑140 (MQ=255)
                                                                                                                         aTGTCGACGCCTGCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGaata                 >  2:3514177/1‑140 (MQ=255)
                                                                                                                         aTGTCGACGCCTGCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGATTCGCCCAAGCTTTGGGTACCTACTGTTTCGCAGGATCGGCCCAGAAATCAGcata                 >  2:3582890/1‑140 (MQ=255)
                                                                                                                              gACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGaa            >  1:4446195/1‑140 (MQ=255)
                                                                                                                                cGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAg          >  2:3319544/1‑140 (MQ=255)
                                                                                                                                cGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAg          >  2:2103517/1‑140 (MQ=255)
                                                                                                                                        ttGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGCCAg  <  1:4285948/140‑1 (MQ=255)
                                                                                                                                        ttGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGCCAg  <  2:3992100/140‑1 (MQ=255)
                                                                                                                                        ttGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGCCAg  <  2:3719873/140‑1 (MQ=255)
                                                                                                                                        ttGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGCCAg  <  2:3647393/140‑1 (MQ=255)
                                                                                                                                        ttGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGCCAg  <  2:1727639/140‑1 (MQ=255)
                                                                                                                                        ttGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTGAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGCCAg  <  1:827275/140‑1 (MQ=255)
                                                                                                                                        ttGATCATCTTTGGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGCCAg  <  2:3911547/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                            
AATACCTGGGTCTGTCTCCCTGACCGCGCGGCTTCCTTCATTAAATAATTGGCTCATTTTCGCCCAATCGGTTTCACCTGCAAATGCCCCTGTCGTTTCATTTCCGACCTGAACCATGCCAATGTCGACGCCTTCCTTGATCATCTTTTGCAGGCTTTGTTTCGTATACTCATAGAGCTTTGTTTTTTTAGCTTCAAAGCTGAGCTTCGCCCAAGCTTTGGGTACCTTCTGTTTCGCAGGATCGGCCCAGAAATCAGAATAATGAAAGTCGGCCAG  >  NZ_CP034943/3319625‑3319900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: