Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,369,578 C→A 16.4% G110W (GGG→TGG)  EO946_RS17410 ← HAMP domain‑containing histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,369,5780CA16.4% 697.0 / 116.4 290G110W (GGG→TGG) EO946_RS17410HAMP domain‑containing histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (160/82);  new base A (36/12);  total (196/94)
Fisher's exact test for biased strand distribution p-value = 3.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

TCCATTCTTCCCTTCTTGAATCATTAAGGAAATGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATAAGTCAGAAAGGAAAATTGCC  >  NZ_CP034943/3369441‑3369715
                                                                                                                                         |                                                                                                                                         
       ttcCCTTCTTGAATCATTAAGGAAATGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGtt                                                                                                                                  >  1:2053892/1‑140 (MQ=255)
         cccTTCTTGAATCATTAAGGAAAAGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGa                                                                                                                                >  1:4165780/1‑140 (MQ=255)
          ccTTCTTGAATCATTAAGGAAATGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGAt                                                                                                                               <  2:106432/140‑1 (MQ=255)
          ccTTCTTGAATCATTAAGGAAATGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGAt                                                                                                                               <  1:3019966/140‑1 (MQ=255)
          ccTTCTTGAATCATTAAGGAAATGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGAt                                                                                                                               <  1:3908653/140‑1 (MQ=255)
          ccTTCTTGAATCATTAAGGAAATGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGAt                                                                                                                               <  2:187416/140‑1 (MQ=255)
          ccTTCTTGAATCATTAAGGAAATGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGAt                                                                                                                               <  2:2357763/140‑1 (MQ=255)
                tGAATCATTAAGGAAATGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCAtttt                                                                                                                         <  1:2354023/140‑1 (MQ=255)
                 gAATCATTAAGGAAATGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCAttttt                                                                                                                        >  1:1712941/1‑140 (MQ=255)
                  aaTCATTAAGGAAATGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTg                                                                                                                       <  1:3672624/140‑1 (MQ=255)
                      aTTAAGGAAATGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCa                                                                                                                   <  2:2277744/140‑1 (MQ=255)
                       ttAAGGAAATGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCCGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCaa                                                                                                                  <  2:670425/140‑1 (MQ=255)
                            gAAATGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAgcgc                                                                                                             <  1:803441/140‑1 (MQ=255)
                            gAAATGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAgcgc                                                                                                             <  1:2914982/140‑1 (MQ=255)
                             aaaaGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGcc                                                                                                            <  2:4165780/140‑1 (MQ=255)
                               aTGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTg                                                                                                          >  2:1455332/1‑140 (MQ=255)
                                tGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGc                                                                                                         >  2:3029092/1‑140 (MQ=255)
                                 gACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGct                                                                                                        >  2:1021940/1‑140 (MQ=255)
                                 gACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGct                                                                                                        >  2:2153309/1‑140 (MQ=255)
                                    aGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCtctc                                                                                                     <  1:4081091/140‑1 (MQ=255)
                                      aTACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCgt                                                                                                   >  1:3711278/1‑140 (MQ=255)
                                      aTACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCgt                                                                                                   >  1:1088330/1‑140 (MQ=255)
                                        aCCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCgtgt                                                                                                 >  2:92254/1‑140 (MQ=255)
                                        aCCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCgtgt                                                                                                 >  2:2459117/1‑140 (MQ=255)
                                            gtgtTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGACCAAAGCGCCTGCTCTCGTGTTCtt                                                                                             >  2:4116252/1‑140 (MQ=255)
                                            gtgtTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCAGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCtt                                                                                             >  1:4351013/1‑140 (MQ=255)
                                                      ctggtggACCCACTGAGTGGTGAAGATATAATGCTGTTTTGGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCggg                                                                                   <  2:4973549/139‑1 (MQ=255)
                                                      atGGTGGACCCACTGATTGGTGAAGATATACTGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCAGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCggg                                                                                   <  1:1283113/140‑1 (MQ=255)
                                                      atGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCggg                                                                                   <  2:4224954/140‑1 (MQ=255)
                                                      atGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCggg                                                                                   <  2:1361850/140‑1 (MQ=255)
                                                      atGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCggg                                                                                   <  2:4494915/140‑1 (MQ=255)
                                                      atGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCggg                                                                                   >  1:2255227/1‑140 (MQ=255)
                                                      atGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCAGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCggg                                                                                   <  1:4032042/140‑1 (MQ=255)
                                                         gtggACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCtt                                                                                >  1:3201498/1‑140 (MQ=255)
                                                         gtggACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCtt                                                                                >  1:4407091/1‑140 (MQ=255)
                                                          tggACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCACAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCttt                                                                               <  1:1177679/140‑1 (MQ=255)
                                                            gACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCa                                                                             >  2:3786877/1‑140 (MQ=255)
                                                            gACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCa                                                                             >  1:1990256/1‑140 (MQ=255)
                                                             aCCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCCTTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCaa                                                                            >  2:2630523/1‑140 (MQ=255)
                                                              cccACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAgcgc                                                                                                             >  1:4787923/1‑106 (MQ=255)
                                                              cccACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAgcgc                                                                                                             <  2:4787923/106‑1 (MQ=255)
                                                              cccACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCaaa                                                                           >  2:3089667/1‑140 (MQ=255)
                                                                cACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGa                                                                         <  1:94853/140‑1 (MQ=255)
                                                                 aCTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGAc                                                                        >  1:336724/1‑140 (MQ=255)
                                                                 aCTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGAc                                                                        >  2:1266974/1‑140 (MQ=255)
                                                                 aCTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGAc                                                                        >  2:1143583/1‑140 (MQ=255)
                                                                 aCTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCAGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGAc                                                                        >  1:1488496/1‑140 (MQ=255)
                                                                    gATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTgg                                                                     >  2:4851282/1‑140 (MQ=255)
                                                                    gATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCAGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTgg                                                                     >  1:529046/1‑140 (MQ=255)
                                                                        ggTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCa                                                                             <  2:828186/128‑1 (MQ=255)
                                                                         gTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGAt                                                                <  2:1712941/140‑1 (MQ=255)
                                                                          tGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATg                                                               <  1:1548520/140‑1 (MQ=255)
                                                                          tGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCAGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGagg                                                               >  2:3499844/1‑138 (MQ=255)
                                                                          tGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCAGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATg                                                               <  2:3207356/140‑1 (MQ=255)
                                                                          tGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCAGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATg                                                               >  2:3691227/1‑140 (MQ=255)
                                                                           gAAGATATAATGCTGTGTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATGTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGt                                                              <  1:901644/140‑1 (MQ=255)
                                                                               atatAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCAtt                                                          <  1:2478976/140‑1 (MQ=255)
                                                                                   aaTGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCgaa                                                      <  1:4974724/140‑1 (MQ=255)
                                                                                      gCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCgaagaa                                                   <  1:3569169/140‑1 (MQ=255)
                                                                                      gCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCgaagaa                                                   <  2:336724/140‑1 (MQ=255)
                                                                                         gTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGACGAAGgc                                                >  1:2193807/1‑140 (MQ=255)
                                                                                         gTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGgc                                                >  1:3227291/1‑140 (MQ=255)
                                                                                         gTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATGTGTGTCCAAAGCGCCTGCTCGCGGGTTCTTGGCTTTCGGGGTGTCAAAGACTGGCCGATGTCATTCGAAGAAGgc                                                >  2:1204820/1‑140 (MQ=255)
                                                                                          tttttGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCtctc                                                                                                     >  2:4606739/1‑86 (MQ=255)
                                                                                          tttttGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCtctc                                                                                                     <  1:3866146/86‑1 (MQ=255)
                                                                                          tttttGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGgcg                                               >  1:242610/1‑140 (MQ=255)
                                                                                          tttttGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGgcg                                               >  2:88646/1‑140 (MQ=255)
                                                                                                             tGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGtttttt                            >  1:1396550/1‑140 (MQ=255)
                                                                                                             tGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGtttttt                            >  2:4815160/1‑140 (MQ=255)
                                                                                                             tGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGtttttt                            >  2:4698935/1‑140 (MQ=255)
                                                                                                              gTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTg                           >  2:2422510/1‑140 (MQ=255)
                                                                                                              gTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTg                           >  2:4523159/1‑140 (MQ=255)
                                                                                                               tAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGa                          >  2:1517473/1‑140 (MQ=255)
                                                                                                                 agagCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCt                                                                                              >  2:1475799/1‑70 (MQ=255)
                                                                                                                 agagCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACa                        >  1:1033369/1‑140 (MQ=255)
                                                                                                                 agagCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACa                        >  2:277531/1‑140 (MQ=255)
                                                                                                                 agagCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACa                        >  1:639989/1‑140 (MQ=255)
                                                                                                                  gagCCTGCCTTCGTACTGCTGCCAGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACAt                       >  1:2792426/1‑140 (MQ=255)
                                                                                                                    gcctgcctTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATaa                     >  1:263880/1‑140 (MQ=255)
                                                                                                                     cctgcctTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATAAg                    >  1:1627978/1‑140 (MQ=255)
                                                                                                                     cctgcctTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATAAg                    >  1:1756893/1‑140 (MQ=255)
                                                                                                                     cctgcctTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATAAg                    >  1:606028/1‑140 (MQ=255)
                                                                                                                      ctgcctTCGTACTGCTGCCAGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATAAGt                   >  2:4040767/1‑140 (MQ=255)
                                                                                                                       tgcctTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATAAGTc                  >  1:3074195/1‑140 (MQ=255)
                                                                                                                       tgcctTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATAAGTc                  >  1:4100211/1‑140 (MQ=255)
                                                                                                                       tgcctTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATAAGTc                  >  2:1289564/1‑140 (MQ=255)
                                                                                                                       tgcctTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATAAGTc                  >  1:1912213/1‑140 (MQ=255)
                                                                                                                           ttCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATAAGTCAGaa              <  1:4834128/140‑1 (MQ=255)
                                                                                                                             cGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATAAGTCAGAAAg            >  2:1791096/1‑140 (MQ=255)
                                                                                                                             cGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATAAGTCAGAAAg            >  2:2318712/1‑140 (MQ=255)
                                                                                                                             cGTACTGCTGCCAGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATAAGTCAGAAAg            >  1:4626505/1‑140 (MQ=255)
                                                                                                                                   gctgcCCGAGTGCGTTGATGATTTTTGTCCAAAGCGACTGCTCTGGTGTTCTTTGCTTTCGGGCTTTCAAATACTGGCCGATGTCATTCGAAGAAAGCGATTGTTCAGAACGTTTTTTGACATAAGTCAGAAAGGAAAAt      >  2:524522/1‑140 (MQ=255)
                                                                                                                                   gctgcCAGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGa                                                                         <  2:586204/73‑1 (MQ=255)
                                                                                                                                   gctgcCAGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATAAGTCAGAAAGGAAAAt      >  1:1259119/1‑140 (MQ=255)
                                                                                                                                    ctgcCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATAAGTCAGAAAGGAAAAtt     >  2:1013158/1‑140 (MQ=255)
                                                                                                                                    ctgcCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGGGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACAAAAGTCAGACAGGAAAAtt     >  2:2927801/1‑140 (MQ=255)
                                                                                                                                      gcCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCggg                                                                                   <  1:268858/60‑1 (MQ=255)
                                                                                                                                       cccGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATAAGTCAGAAAGGAAAATTGcc  <  2:1943538/140‑1 (MQ=255)
                                                                                                                                         cGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGc                                                                    <  2:3257943/72‑1 (MQ=255)
                                                                                                                                         cGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGc                                                                    >  1:3257943/1‑72 (MQ=255)
                                                                                                                                         |                                                                                                                                         
TCCATTCTTCCCTTCTTGAATCATTAAGGAAATGACAGATACCGGTGTTTTCATATGGTGGACCCACTGATTGGTGAAGATATAATGCTGTTTTTGCTGATTGATATATTGTAAGAGCCTGCCTTCGTACTGCTGCCCGAGTGCGTTGATCATTTTTGTCCAAAGCGCCTGCTCTCGTGTTCTTGGCTTTCGGGCTTTCAAAGACTGGCCGATGTCATTCGAAGAAGGCGCTTGTTCCGCAAGTTTTTTGACATAAGTCAGAAAGGAAAATTGCC  >  NZ_CP034943/3369441‑3369715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: