Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,447,038 T→G 11.2% N183T (AAC→ACC)  degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,447,0380TG11.2% 696.7 / 14.0 364N183T (AAC→ACC) degUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base T (127/177);  new base G (4/56);  total (131/233)
Fisher's exact test for biased strand distribution p-value = 2.01e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 383 total aligned reads displayed.

TCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTCGT  >  NZ_CP034943/3446899‑3447176
                                                                                                                                           |                                                                                                                                          
tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTAACGGTTTTCTCACTGATAAACAATGATTAACCAATACCGCGGt                                                                                                                                            >  2:627719/1‑140 (MQ=255)
tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGt                                                                                                                                            <  2:2007705/140‑1 (MQ=255)
tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTTCTCACATTGTTTTTCACGGTTTTCTCAATTATAAAAAATGATTAAACCATAACGCGGt                                                                                                                                            >  2:2825158/1‑140 (MQ=255)
  tcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTTCTCACATTGTTTTTCACGGTTTTATAACTGATAAAAAAAGATTAAACAAAACCCCGGttt                                                                                                                                          >  1:3227292/1‑139 (MQ=255)
  tcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACAATCATTTTTTTCAAAAAATTTCTCACATGGTTTTTCACGGTTTTATCACTGATAAAAAAAGATTCAACAAAAACCCGGTTg                                                                                                                                          >  1:458108/1‑140 (MQ=255)
     tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTCATTCATTTTTTTCAAAAAATTTCTAAAATTGTTTTTCACGGGTTTGTTACTGATACACAATGATTGCCCAATAAAGCGGTTGcgt                                                                                                                                       >  1:3866595/1‑138 (MQ=255)
     tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATAATTCTCACATGGTTTTTAAAGGTTTTGTCACTGATAAACAATGATTCACCAAAACCGCggttggtt                                                                                                                                       >  1:984001/1‑140 (MQ=255)
        cTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCttttt                                                                                                                                    <  2:163295/140‑1 (MQ=255)
              aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTCACGGTTTTATGACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTACGTCt                                                                                                                              >  2:3581775/1‑140 (MQ=255)
              aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTTGAAAATATTTCTCACATTGTTTTTAACGGTTTTCTAACTTATAAACAAAGATTAACCAAAACCGCCGTTGGTTTTTAAGTCt                                                                                                                              >  1:2268207/1‑140 (MQ=255)
                ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTCATTGATTTTTTTCAAAATATTTGTGACATGGGTTTTCACGGGTTTATGACTGATAAACAATGGTTGACCAATAACGCGGTTGCTTTTTGCGGCTGc                                                                                                                            >  1:4144838/1‑140 (MQ=255)
                 cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                                                                                           <  1:4216199/140‑1 (MQ=255)
                 cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCa                                                                                                                           <  1:3112269/140‑1 (MQ=255)
                 cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATATTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAAGACCGCGGGTGCTTTTTCCGTCTGCa                                                                                                                           <  1:1076891/140‑1 (MQ=255)
                    ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                                                                        <  1:3517167/140‑1 (MQ=255)
                    ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACAGGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                                                                        <  1:4334933/140‑1 (MQ=255)
                          tGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTTCAAAAAATTTCTCACATGGTTTTTCACGGTTTTGTCACTGCTAAACAAAGATTAAACAATACCCCGGTTGGTTTTTGCGTCGGCCAGccacagc                                                                                                                  >  1:302201/1‑134 (MQ=255)
                            gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTGTCCGTCTGCAAGCATCTgcag                                                                                                                <  2:2262306/140‑1 (MQ=255)
                              gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagca                                                                                                              <  1:897466/140‑1 (MQ=255)
                              gACCACAACGGCTTGCGTCCGGTCGTGTAAAATCATTTTTTTCAAAATATTGCTCACATTGTTTTTAAAGGTTTTTTGACTGTTAAAAAAAGATTAACCAAAACCGCGGTTGCTTTTTACGTATTCAAGCAAATgcagca                                                                                                              >  1:2804542/1‑140 (MQ=255)
                               aCCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATTGTTTTTCACGGTTTTATCACAGATAAAAAAAGATTAACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCAc                                                                                                             >  2:3991687/1‑140 (MQ=255)
                               aCCACAACGGCTTGCGTCCGGTCGTGTACCTTAATTTTTTGCAAAATATTGCTCACATGGTTTTTACCGGTTTTATCACTGATAAACAATGATTAACCAAAACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCAc                                                                                                             >  2:2095336/1‑140 (MQ=255)
                                         cTTGCGTCCGGTCGTTTACCTTCATTTTTTGCAAAAAATTGCTCACATGGGTTTTCACGGTTTTCTCACTGATAAACAATGATTGAACAATACCGCGGTTGGTTTTTCCGTCTGCAAGCATCTGCCGCCCTTCACAGTcc                                                                                                   >  2:1267168/1‑140 (MQ=255)
                                         cTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTAACGGTTTTCTCACTGATAAAAAATGATTAACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACATCACATTcc                                                                                                   >  2:4235283/1‑140 (MQ=255)
                                             cGTCCGGTCTTTTACATTCATTTTTTGCTAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATTATTCACCAATACCGCGGTTGCTTTTTCCGTCTGGAAGCATCTGCTGCACTTCACATTCccgcc                                                                                               <  2:1095957/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  1:4857746/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  2:3779682/140‑1 (MQ=255)
                                                     agTTTACATTCATTTTTTGCAAAATATTGCTCACATGTTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  1:4037956/139‑1 (MQ=255)
                                                       tttACATTCCTTTTTTGCAAAATATTGCTCACATGCTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                                                                                     <  2:3685155/140‑1 (MQ=255)
                                                       tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                                                                                     <  2:4880402/140‑1 (MQ=255)
                                                       tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                                                                                     <  1:222662/140‑1 (MQ=255)
                                                            attcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa                                                                                <  1:2665944/140‑1 (MQ=255)
                                                            attcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa                                                                                <  1:3987987/140‑1 (MQ=255)
                                                            attcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa                                                                                <  1:3939187/140‑1 (MQ=255)
                                                            attcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAAGACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa                                                                                <  1:664610/140‑1 (MQ=255)
                                                             ttcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTATGCAAGCATCTGCAGCACTTCACATTACATCAGAGTTAAAATATGTaa                                                                               <  1:464385/140‑1 (MQ=255)
                                                                      tGCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATAGCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCAGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  1:64632/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      >  2:557382/1‑140 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  2:2477742/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  2:838001/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  1:4745917/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTGTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  2:4590385/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  2:3220231/140‑1 (MQ=255)
                                                                      tGCAAAATAGTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTGGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  2:1356926/140‑1 (MQ=255)
                                                                      ggCAAAATATTGCTCACATGGTTTGTCACGGTTTTCTGACTGATAAACAATGATTGACCAATACCGCGGGGGAGTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGGCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  1:52044/139‑1 (MQ=255)
                                                                           aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                                                                 <  1:750224/140‑1 (MQ=255)
                                                                           aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                                                                 <  1:1533906/140‑1 (MQ=255)
                                                                           aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                                                                 <  2:3897321/140‑1 (MQ=255)
                                                                           aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                                                                 <  1:564085/140‑1 (MQ=255)
                                                                                tGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  2:1402034/113‑1 (MQ=255)
                                                                                tGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGa                                                            <  2:4304142/140‑1 (MQ=255)
                                                                                 gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTTCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                                                           <  1:234135/140‑1 (MQ=255)
                                                                                 gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                                                           <  1:2545319/140‑1 (MQ=255)
                                                                                 gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                                                           <  2:69772/140‑1 (MQ=255)
                                                                                 gCTCACATGGTTTGTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                                                           <  2:2747376/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:101095/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     >  1:1806835/1‑140 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:3031411/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:2073758/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:1550746/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:1739123/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     >  1:3304381/1‑140 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:1437318/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:1165310/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:4286283/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:1986836/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGGGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:1620478/140‑1 (MQ=255)
                                                                                       aTGGTTGTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTGTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGACTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:3530391/140‑1 (MQ=255)
                                                                                         ggTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCa                                                   <  1:3581775/140‑1 (MQ=255)
                                                                                         ggTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCa                                                   <  1:1370373/140‑1 (MQ=255)
                                                                                            ttttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGc                                                <  1:4428186/140‑1 (MQ=255)
                                                                                            ttttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGc                                                >  2:2198869/1‑140 (MQ=255)
                                                                                              ttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTg                                              <  1:1337427/140‑1 (MQ=255)
                                                                                                  cGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAggg                                          <  2:4429754/140‑1 (MQ=255)
                                                                                                  cGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAggg                                          <  1:3662607/140‑1 (MQ=255)
                                                                                                  cGGTTTTCTCACTGAGAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAggg                                          <  2:2962823/140‑1 (MQ=255)
                                                                                                  cGGTTTTCTAACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAggg                                          <  2:535616/140‑1 (MQ=255)
                                                                                                    gTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGgtg                                        <  2:4754388/140‑1 (MQ=255)
                                                                                                    gTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGCCCTCATGCTGAGGgtg                                        <  2:469410/140‑1 (MQ=255)
                                                                                                    gTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGgtg                                        <  2:2435364/140‑1 (MQ=255)
                                                                                                                   aaCAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCt                         <  2:1776402/140‑1 (MQ=255)
                                                                                                                   aaCAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCt                         <  2:2804511/140‑1 (MQ=255)
                                                                                                                   aaCAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCt                         <  2:4466106/140‑1 (MQ=255)
                                                                                                                        tGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACtt                                                                                                           >  2:1447301/1‑53 (MQ=255)
                                                                                                                        tGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     >  2:749819/1‑107 (MQ=255)
                                                                                                                        tGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCttgttg                    >  2:2282309/1‑140 (MQ=255)
                                                                                                                          aTTCACCAATACCGCGGTTGCTTTTTCCGTCTTCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCa                  >  1:833214/1‑140 (MQ=255)
                                                                                                                          aTTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCa                  >  1:2156824/1‑140 (MQ=255)
                                                                                                                                cAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAggcgg            >  1:341092/1‑140 (MQ=255)
                                                                                                                                cAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAggcgg            >  2:3255378/1‑140 (MQ=255)
                                                                                                                                cAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAggcgg            >  1:1212224/1‑140 (MQ=255)
                                                                                                                                cAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAggcgg            <  1:4904874/140‑1 (MQ=255)
                                                                                                                                    aCCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGa        <  2:4783968/140‑1 (MQ=255)
                                                                                                                                        cgGTTGGTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTTAAATATGTCATGGGCTGCGGAGGTCAGGATAGACGTCATGCTGAGGGGGTGCAGAAACGCGGCTTGTTGCAAGGCGGCGGACTTc    >  1:2910286/1‑140 (MQ=255)
                                                                                                                                        cgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAg                                                         >  2:4403055/1‑87 (MQ=255)
                                                                                                                                        cgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGACTTc    >  2:4340535/1‑140 (MQ=255)
                                                                                                                                        cgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTc    >  1:2641358/1‑140 (MQ=255)
                                                                                                                                        cgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTc    >  2:2602521/1‑140 (MQ=255)
                                                                                                                                        cgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCAAATTCCCGCCGAGGTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTc    >  1:2441191/1‑140 (MQ=255)
                                                                                                                                          gTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATCTGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCACGGCGGCGGAATTCGt  >  2:4631755/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTCGT  >  NZ_CP034943/3446899‑3447176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: