Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,769,576 T→G 8.3% I281S (ATT→AGT)  sacX → SacY negative regulator SacX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,769,5760TG 8.3% 825.2 / 16.3 348I281S (ATT→AGT) sacXSacY negative regulator SacX
Reads supporting (aligned to +/- strand):  ref base T (125/180);  new base G (39/4);  total (164/184)
Fisher's exact test for biased strand distribution p-value = 3.10e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 366 total aligned reads displayed.

AAGCTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCTGCTTGCTGATCCGCGTGTCGG  >  NZ_CP034943/3769437‑3769711
                                                                                                                                           |                                                                                                                                       
 aGCTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAtt                                                                                                                                        <  1:1033816/140‑1 (MQ=255)
 aGCTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAtt                                                                                                                                        <  1:1461872/140‑1 (MQ=255)
   cttttAAAATCAATTGGGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTTTGACACACTCGATTGt                                                                                                                                      <  2:2534347/137‑1 (MQ=255)
   cTATTAAAATCCAGTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGGGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGt                                                                                                                                      <  2:3292622/140‑1 (MQ=255)
   cTATTAAAATCAATTGTTCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTAAGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGt                                                                                                                                      <  2:1827803/140‑1 (MQ=255)
   cTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGt                                                                                                                                      <  2:1056964/140‑1 (MQ=255)
   cTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGt                                                                                                                                      <  1:813094/140‑1 (MQ=255)
   cTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGt                                                                                                                                      <  1:3858531/140‑1 (MQ=255)
    tATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTc                                                                                                                                     <  2:3022805/140‑1 (MQ=255)
     aTTAAACTCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCt                                                                                                                                    <  2:143370/140‑1 (MQ=255)
     aTTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCt                                                                                                                                    <  2:1388995/140‑1 (MQ=255)
      ttAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCta                                                                                                                                   <  2:3645026/140‑1 (MQ=255)
        aaaaTCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCtata                                                                                                                                 <  1:3444283/140‑1 (MQ=255)
        aaaaTCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCtata                                                                                                                                 <  1:604038/140‑1 (MQ=255)
              aaTTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTAtt                                                                                                                           >  1:2601424/1‑139 (MQ=255)
              aaTTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATg                                                                                                                           >  1:3411662/1‑140 (MQ=255)
                ttGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTTTCTATATTTATGAt                                                                                                                         >  1:2111254/1‑140 (MQ=255)
                         aGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGc                                                                                                                <  2:1315616/140‑1 (MQ=255)
                             gCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTTATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAttg                                                                                                            <  1:1538099/140‑1 (MQ=255)
                             gCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATTATAAAGCCGGCAttt                                                                                                            >  1:4679512/1‑139 (MQ=255)
                             gCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAttg                                                                                                            >  1:4149228/1‑140 (MQ=255)
                             gCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAttg                                                                                                            <  1:4066889/140‑1 (MQ=255)
                             gCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGAGTGTGATGAATCCCGCCGCATCTATCATCGGCCAATTTATGACACAGTCGCTGGTGTAGAGTTATGATCACCCCGGCAgtg                                                                                                            >  1:2745149/1‑140 (MQ=255)
                              cTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAGTGTCTATATTTATGATAAAGCCGGCaattt                                                                                                           >  2:1584267/1‑136 (MQ=255)
                                      cgtcATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAg                                                                                                   <  2:4107244/140‑1 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                 <  2:3411871/140‑1 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                 <  2:561137/140‑1 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                 <  2:4395879/140‑1 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                 <  1:4363028/140‑1 (MQ=255)
                                               tCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTcg                                                                                          <  1:3927992/140‑1 (MQ=255)
                                               tCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTcg                                                                                          <  2:735141/140‑1 (MQ=255)
                                                 cTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTcggc                                                                                        <  1:18679/140‑1 (MQ=255)
                                                 cTTTTAGTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTcggc                                                                                        <  1:4036343/140‑1 (MQ=255)
                                                  ttttATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACAGAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTcggcg                                                                                       <  2:2477002/140‑1 (MQ=255)
                                                    ttattaCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCggcggc                                                                                     <  2:452793/140‑1 (MQ=255)
                                                      attaCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAtt                                                                                                                                        <  2:2255609/87‑1 (MQ=255)
                                                           ggTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTGGGCGGCATCTAca                                                                              >  2:3405061/1‑139 (MQ=255)
                                                           ggTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATAAAGCCGGCATTGTTTCCGGAGCCCTGTTAGGCGGCATCTAca                                                                              >  1:1305307/1‑139 (MQ=255)
                                                           ggTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATAATTATTAACACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACt                                                                              >  1:4967541/1‑140 (MQ=255)
                                                           ggTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATAATTATGATAACGCCGGCAATGTTGCCGGAGCCCTGTTCGGCGGCATCTACt                                                                              >  2:1883888/1‑140 (MQ=255)
                                                            gTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACCCAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTc                                                                             <  1:2323614/140‑1 (MQ=255)
                                                            gTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTc                                                                             <  1:3345544/140‑1 (MQ=255)
                                                            gTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTc                                                                             <  1:3091638/140‑1 (MQ=255)
                                                            gTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTc                                                                             <  1:3890922/140‑1 (MQ=255)
                                                               aTGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTAGGCGGCATCTACTccac                                                                          >  2:445790/1‑140 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                        <  2:2899865/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                        <  2:2107377/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                        <  2:1476847/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                        <  2:2703485/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                        <  2:358176/140‑1 (MQ=255)
                                                                    gccAGGGTGCCTCGCTCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGTCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCg                                                                     <  2:3675727/138‑1 (MQ=255)
                                                                       aGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAGTGTTGCCGGAGCCCTGGTAGGCGGCCTCTACTACACCATCGGTc                                                                  >  2:4575185/1‑140 (MQ=255)
                                                                       aGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATAATTATTATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTACACCAACGttg                                                                  >  2:4215114/1‑139 (MQ=255)
                                                                         ggTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATTATCACCCCGGCATTTTTGCCGGAGCCCGGTTCGGCGGCATCTACTCCACCATAGTtata                                                                >  1:3721999/1‑140 (MQ=255)
                                                                         ggTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATTATCAAGCCGGCATTTTTGCCCGAGCCCGGTACGGCGGCATCTAAACAACCAACGTTCTa                                                                >  1:1721096/1‑140 (MQ=255)
                                                                         ggTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTa                                                                >  1:4295917/1‑140 (MQ=255)
                                                                         ggTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAGTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCGGTTCGGCGGCCTCTACTCCACCCACGTTCTa                                                                >  1:2291873/1‑140 (MQ=255)
                                                                          gTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTAt                                                               <  2:3865/140‑1 (MQ=255)
                                                                          gTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTAt                                                               <  2:3975940/140‑1 (MQ=255)
                                                                          gTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTAt                                                               <  2:3767222/140‑1 (MQ=255)
                                                                           tGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATc                                                              <  2:4155479/140‑1 (MQ=255)
                                                                           tGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATc                                                              <  2:3904462/140‑1 (MQ=255)
                                                                           tGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATGTATGATCAAGCCCGCATTGTTGCCCGAGCCCTGGTCGGCGGCATCTACGCCCCCCTCggtgactc                                                              >  2:2774864/1‑133 (MQ=255)
                                                                           tGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATGATGACACAGTCGCTTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCCCCATCTTTCTATc                                                              <  1:2234809/140‑1 (MQ=255)
                                                                             ccTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAt                                                                                                                                         <  1:4591071/63‑1 (MQ=255)
                                                                               tCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGc                                                          <  2:207730/140‑1 (MQ=255)
                                                                               tCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGc                                                          <  1:2781227/140‑1 (MQ=255)
                                                                                cgcgCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCt                                                         <  2:1161715/140‑1 (MQ=255)
                                                                                cgcgCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCt                                                         <  2:1987818/140‑1 (MQ=255)
                                                                                 gcgcTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAAACATGCCGGCGCCCGGTTCGGCGGCATCTAATACACCAACGGTCTATCCGGCtt                                                        >  2:2187352/1‑140 (MQ=255)
                                                                                 gcgcTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCGATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTAATCCACCATCGTTCTATCCGGCtt                                                        >  2:2578770/1‑140 (MQ=255)
                                                                                       tttGTGTTGAATCCCGCCGCATCTATCATCGGCAAAATTATGACACAGTCGATTGTATATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATATACTCCACCATCGTTCTATCCGGCTTGcatca                                                  <  1:1192805/139‑1 (MQ=255)
                                                                                       aTTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGcatca                                                  <  1:3588882/140‑1 (MQ=255)
                                                                                       aTTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGcatca                                                  <  1:2995187/140‑1 (MQ=255)
                                                                                       aTTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGcatca                                                  <  1:1396070/140‑1 (MQ=255)
                                                                                       aTTGGGATGAATCCCGCCGCCTCTATCATCGGCCAAATTAGGACACACTCGATTGTCTCTATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTGGcatca                                                  <  1:2680936/140‑1 (MQ=255)
                                                                                             atgaATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAGTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCGGTTCGGCGGCATCTACTCCACCATCCTTCTATCCCGCTTGCATCAAAGcgt                                            >  2:4508919/1‑138 (MQ=255)
                                                                                                 aTCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTAt                                        <  1:2521054/140‑1 (MQ=255)
                                                                                                 aTCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTAt                                        <  2:3216630/140‑1 (MQ=255)
                                                                                                 aTCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTAt                                        <  1:4215114/140‑1 (MQ=255)
                                                                                                 aTCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTAATATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATAATAGCTTTTAt                                        >  2:2327735/1‑140 (MQ=255)
                                                                                                      gcctcttCTATCATCGGCCAATTTATGACACAGTCTATTGTCTATATTTATGATCACTCCGTCATTGTTGCCGGAGCCCTGTTCGGCGGTATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGAGAt                                   <  2:2783238/134‑1 (MQ=255)
                                                                                                        cgcATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTg                                 <  1:1262506/140‑1 (MQ=255)
                                                                                                        cgcATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTg                                 <  1:637751/140‑1 (MQ=255)
                                                                                                              tATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCaa                           <  1:2924117/140‑1 (MQ=255)
                                                                                                               atcatcGGCCAAATTATGACACAGTCGATGGTCTATTTTTATGATCCCGCCGGCATTGTTGCCCGAGCCCGGTTCGGCGGCATCTACTCCACCATAGTTCTATCCGGCGTGCATCATAGCTGTTATGCGATTGAAGCAAc                          >  2:3102945/1‑140 (MQ=255)
                                                                                                                  atcGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACgct                       <  2:285494/140‑1 (MQ=255)
                                                                                                                  atcGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACgct                       <  2:4769851/140‑1 (MQ=255)
                                                                                                                  atcGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACgct                       <  2:1680835/140‑1 (MQ=255)
                                                                                                                     ggCCAAATTATGACACAGTCGATTGTCTATATTTATGTTCACGCCGGCCGTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATTGCGTTTATGCGCTTGTAGCAACgctgcg                    >  2:4331991/1‑139 (MQ=255)
                                                                                                                      gCCAAATTATGACACAGTCGATTGTGTCTATTTATGATCACGCCGGCATTGTTGCCGGAGCCCGGTGCGGCGGCCTCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCtgctt                   >  1:247662/1‑140 (MQ=255)
                                                                                                                      gCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCGGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCtgctt                   >  2:1115296/1‑140 (MQ=255)
                                                                                                                      gCCAAATTATGACACAGTCGATTGGCTATAATTAAGATAACGCCGGCAATGTTGCCGGACCCCTGTTCGGCGGCATCTAATCCAACATCGTTATATACGGCGTGCAAGCTAACGATTATGAGATTGAAGGAACgcagcaa                   >  2:4862213/1‑138 (MQ=255)
                                                                                                                      gCCAAATTATGACACAGTCGATGGTCGATATTTATGATCACGCCCGCAATTTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCAACAGCGTTGTATACGGCTTGCATCATAGCTTTTATGCGATTGAAGCAAAGCtgctt                   >  2:3320149/1‑140 (MQ=255)
                                                                                                                      gCCAAATTATGACACAGTCGAGTGTCTATATTTATGATCACGCCCGCATTGTTTCCGGAGCCCTGTTCGGCGGCATCTAATCCACCATCGGTCTATCCGGCTTTGATCAAAGCTTTTATTCGATTTAAGCAACcctgcgt                   >  1:1601071/1‑138 (MQ=255)
                                                                                                                          aaTTATGACACAGTCGAGTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCCACGTTCTATCCGGCTTGCATCATATCTTTTATGCGCTTGAAGCAACGCTGCTTGCTg               >  2:615837/1‑140 (MQ=255)
                                                                                                                            ttATGACACAGTCGAGTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCTGCTTGCTGAt             <  1:4484405/140‑1 (MQ=255)
                                                                                                                                       tCGATTGTCTATATTTATGATCACGCCGGCAGTGTTTCCGGAGCCCTGTTCCGCGGCATATACTCCACCAACGTTCTATCCGGCTTGCATAATAGCGTTTATTCGATTGAAGCAACGCTGCATGCTGATCCGCGTGTCgg  >  2:690208/1‑140 (MQ=255)
                                                                                                                                       tCGATTGTCTATATTTATGATCACGCCCGCATTGTTGCCGGAGCCCGGTTCGGCGGCAACTACTCCACCATCGTTCTATACGGCTTGCCTCATAGCGTTTATGCGATTGAAGCAACGCTGCTTTCTGAACCCCGTGTCgg  >  2:2021833/1‑140 (MQ=255)
                                                                                                                                       tCGAGTGTCTATATTTATTATAACGCCGGCATTTTTTCCGGACCCCTGTTCGGCGGCATCTACTCCACCATAGTTCTATCCGGCTTGAATCATAGCGTTTATTCGATTGAAGCAAACCTGCTTTCTGATCCGCGTGTCgg  >  2:1934701/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                       
AAGCTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCTGCTTGCTGATCCGCGTGTCGG  >  NZ_CP034943/3769437‑3769711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: