BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,359	A→C	16.1%	T68P (ACC→CCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,359	0	A	C	16.1%	423.1 / 28.7	250	T68P (ACC→CCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (83/114); new base C (6/40); total (90/160)
Fisher's exact test for biased strand distribution p-value = 1.51e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

CGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607225‑607498

 gggAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAg                                                                                                                                       >  1:1938432/1‑140 (MQ=255)

      cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGCCg                                                                                                                                  >  1:3525408/1‑140 (MQ=255)

      cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  <  1:4765272/140‑1 (MQ=255)

      cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  <  1:554089/140‑1 (MQ=255)

      cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  <  2:547055/140‑1 (MQ=255)

      cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  <  1:3439382/140‑1 (MQ=255)

      cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  <  1:247632/140‑1 (MQ=255)

        aCTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACggg                                                                                                                                >  1:3013064/1‑140 (MQ=255)

               aaGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGCTGGACCAGCCGGAGCGACGGGCGCCCCAGGAGCGACCGGGCAAGCCGGACCATCCCGCACACCCGGAGCGACCGGAGCCCCg                                                                                                                         >  2:612304/1‑140 (MQ=18)

               aaGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACg                                                                                                                         >  2:1959607/1‑140 (MQ=255)

                aGCTGGACCAGCCGGACCAGCCAGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACgg                                                                                                                        <  1:1061012/140‑1 (MQ=255)

                 gCTGGACCAGCCGGACCCGCCGGAGCGAGGGGAGCCACGGGAGCTCCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACggg                                                                                                                       <  2:3854043/140‑1 (MQ=18)

                 gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCCCGGGAGCTCCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACggg                                                                                                                       <  2:67684/140‑1 (MQ=255)

                 gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       >  1:82164/1‑140 (MQ=255)

                 gCTGGACCAGCCGGACCAGCCGGAGCCACGGGAGCCACGGGAGCTACCGGGGAAGCGGGACCAGCCGGAGCGACGGGAGCCACAGGCGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACggg                                                                                                                       <  2:2066322/140‑1 (MQ=18)

                   tGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGCAGCGACGGGAGCGACAGGAGCTACCGCGGACGCCGGACCAGCCGCACCAACCGGAGccacgggagccacgggag                                                                                                                     >  2:3360329/1‑140 (MQ=21)

                      accagccggaccagccggAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTa                                                                                                                  >  1:4487947/1‑140 (MQ=255)

                           ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                             <  1:4410616/140‑1 (MQ=255)

                           ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                             <  1:4163670/140‑1 (MQ=255)

                               cccagccggaGCGACGGGAGCCACGGGAGCCACCGGCGAAGCGGGACCAGCCGGATCTGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGCCGGACCATCCGGAGCGAGTGGAGCCACGGGAGCTACCGGGGAag                                                                                                         <  2:2756903/139‑1 (MQ=18)

                               accagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAag                                                                                                         <  2:3409727/140‑1 (MQ=255)

                               accagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGGCCTGCCGGAGCTACACGACCGACGGGCGCCACGGGAGCGCCCGGGGacg                                                                                                         >  2:4328639/1‑138 (MQ=18)

                                    ccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGTAGCAGGACCAGCCGGACCCACCGGAGCGACGGGAGCGCGGGGGCCTGCCGGGGGagccgga                                                                                                    >  1:1001482/1‑140 (MQ=18)

                                         gCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagc                                                                                               >  2:4833983/1‑140 (MQ=255)

                                             cGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCTACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgga                                                                                           <  2:2543983/140‑1 (MQ=255)

                                             cGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgga                                                                                           <  2:4880533/140‑1 (MQ=255)

                                             cGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgga                                                                                           <  2:1732991/140‑1 (MQ=255)

                                             cGGGAGCCACGGGAGCACCCGGCGAAGCAGGACCAGCCGGAGCGACGGGAGCGACACGAGCAACCGGGGACCCAGCACCATCCGGAGCAACCGGAGCGACGGGGGCCACGGGGGCTCCCGGGGCcgccggaccggcggga                                                                                           >  1:4638849/1‑140 (MQ=16)

                                              gggAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccggac                                                                                          <  2:1703760/140‑1 (MQ=255)

                                              gggAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACCGGAGCGACAGGAGCTACCGGGCAAGCCGGACAAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCAACCGGCACaggcgaagcagccggcg                                                                                          >  1:3700502/1‑138 (MQ=17)

                                                gAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAgccggaccagccggacca                                                                                        <  1:2821115/140‑1 (MQ=255)

                                                gAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAgccggaccagccggacca                                                                                        <  1:2352509/140‑1 (MQ=255)

                                                  gCCACGGGAGCTCCCGGGGCAGCTGGACCAGCCGGAGCGCCGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                                                                      <  2:4543194/140‑1 (MQ=18)

                                                  gCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                                                                      <  2:150915/140‑1 (MQ=255)

                                                  gCCACCGGAGCTACCGGCGAAGCTGGACCAGCCGGCGCGACGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                                                                      <  2:1484353/140‑1 (MQ=255)

                                                       gggAGATACCGGGGCAGCTGGCCCCGCCGGCGCGACGGGAGCGCCAGGAGCTACCGGGGAACCCGGACCAGCCGGACCAACCGGAGCGACGGGCGCCACTGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggag                                                                                 <  2:2450843/140‑1 (MQ=18)

                                                            cTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgaca                                                                            <  1:1340444/140‑1 (MQ=255)

                                                                cGGGGCAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCGACCGGGGCAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggag                                                                        <  2:3998408/140‑1 (MQ=255)

                                                                cGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggag                                                                        <  1:199953/140‑1 (MQ=255)

                                                                   ggAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACGGGGGAAGCAGGACCAGCCGGCGCAACAGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGAGCAGCCGGACCAACCggagcgacaggagcgc                                                                     >  1:2074672/1‑139 (MQ=17)

                                                                    gAATCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  2:2160540/140‑1 (MQ=255)

                                                                    gAAGCTGGACCCGCGGGAGCGACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGCGCTCCCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  1:1078096/140‑1 (MQ=17)

                                                                    gAAGCTGGACCAGCCGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  1:1522474/140‑1 (MQ=255)

                                                                    gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  1:2340797/140‑1 (MQ=255)

                                                                    gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  1:1603622/140‑1 (MQ=255)

                                                                    gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  1:3289562/140‑1 (MQ=255)

                                                                    gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  1:3339286/140‑1 (MQ=255)

                                                                    gAAGCTGGACCAGCCGGAGCCACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACGGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  1:1886284/140‑1 (MQ=255)

                                                                       gCTGGACCAGCCGGAGCGCGGGGCGCGCCAGTTGCTACCGGGGAAGCGGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                 <  1:4328639/140‑1 (MQ=17)

                                                                       gCTGGACCACCCGGAGCGACGGGAGCCACAGGAGCTACCGGCGAACCCGGACCACCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacgggagccacagg                                                                 <  1:4857231/140‑1 (MQ=14)

                                                                         cggACCAGCCGGAGCGCCGGGAGCGACCGGAGCTACCGGCGACGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                               <  2:700795/139‑1 (MQ=255)

                                                                         aggACCAGCCGGAGCGACGGGAGCGACCGGAGCTACCGGTGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                               <  2:4428305/139‑1 (MQ=255)

                                                                                 ccGGCGCGACGGGAGCGACAGGAGCTACCGGCGAACCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAACCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:1691580/140‑1 (MQ=17)

                                                                                 ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACGAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       >  2:2967705/1‑140 (MQ=255)

                                                                                 ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       >  2:1191799/1‑140 (MQ=255)

                                                                                 ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       >  2:3450188/1‑140 (MQ=255)

                                                                                 ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:4747647/140‑1 (MQ=255)

                                                                                     aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   >  1:801346/1‑140 (MQ=255)

                                                                                          cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCCCGGGAGCTACCGGGGAAGCCGGAGCAGCCGGCCCAACCGGAGCGACAGGAGCGCCAGGAGCTACCGGGGAAGCCggc                                              >  2:234313/1‑139 (MQ=255)

                                                                                           gggAGTGACAGGATGAACCGGGGAATCCGGACCAGCCGGCCCATCCTGAGCGACTGGAGCCACGGGAGCTACCGGTGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCAGGAc                                             <  2:2888846/140‑1 (MQ=14)

                                                                                           gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCAACCGCAGCGACGGGAGCCACCGCAGCTCCCGGGGAAGCAGGACCGGCCGGACCACACGGAGAGCCAGGGGCGGCAGGGGGGCCCGGGGACGGCGGag                                             >  2:3748093/1‑139 (MQ=9)

                                                                                           gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCCGGAc                                             >  2:3164158/1‑140 (MQ=255)

                                                                                           gggAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                             <  1:641821/140‑1 (MQ=255)

                                                                                           gggAGCCACCGGAGCGACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                             <  2:2817765/140‑1 (MQ=255)

                                                                                           cggagccacaggagccacaGGCGAACCCGGACCACCCGGACCACCCGGAGCGACGGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                             <  1:4086969/139‑1 (MQ=14)

                                                                                               gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGc                                         >  2:21941/1‑140 (MQ=255)

                                                                                                    cggAGCTCACGGCGAAGCCGGACCAGCAGGACCAACCGGAGCGACTGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCTCCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggag                                    <  2:1176853/139‑1 (MQ=14)

                                                                                                    aGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggag                                    <  2:3222307/140‑1 (MQ=255)

                                                                                                    aGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggag                                    <  2:111842/140‑1 (MQ=255)

                                                                                                         cTACCGGGGAAGCCGGACCAGCCGGAGCAACCGGAGCGACGGGAGCCACGGGAGCTACGGGGGAAGCCGGACCAGCCGGAGCAACCGGAGCGACAGGAGCCACAGGCGCGACCGGGGCAGCCGGTCCCGCCggagcggcg                               >  2:4126286/1‑137 (MQ=14)

                                                                                                           aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCgg                                                                                   <  1:2210697/86‑1 (MQ=255)

                                                                                                           aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCgg                                                                                   >  2:2211986/1‑86 (MQ=255)

                                                                                                           aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCTACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                             >  1:3877932/1‑140 (MQ=255)

                                                                                                           aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCAACCGGGGAACCCGGACCCACCGGACCCACCGGAGCCGCGGTTGACACGGGCGCTCCCGGGCCCTCCGGCCCAGCCggcgcggcagg                             >  2:2683824/1‑140 (MQ=9)

                                                                                                           aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTCCCGGGGAAGCCGGACCAGCCGGAGCTACCGGAGCGCCCGGCGCAGCCGGAGCGTCGGGGGCAGCCGGAGCTGCCggggcggccgg                             >  2:1086762/1‑135 (MQ=9)

                                                                                                           aCCGGCGAATCCGGCCCAGCCGGACCAACCGGAGCGCCGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                             <  2:3124723/140‑1 (MQ=17)

                                                                                                                 gcacccggaccagccggaccaCCCGGAGCGCCGGGAGCCACGGGAGCTACCGGCGAAGCCGGACCAGCCGGACCACCCGGAGCGACCGGAGCGACAGGAGGTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgac                       <  2:784912/136‑1 (MQ=17)

                                                                                                                 gaacaaggaccagcaggaccaACCGGAGCGACGGGAGCCACGGGAGCCACAGGCGCAGCCGGTGCAGCCGGACCACCCGGAGCGACAGGAGCGACCGGAGCTACGGGGGAAGCCGGACCAGCCggagcgacaggagcgac                       <  2:836249/134‑1 (MQ=17)

                                                                                                                 gAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCggagcgacaggagcgac                       <  2:3654573/140‑1 (MQ=255)

                                                                                                                  ctgccggaccagcgggaccaACCGGAGCGACTGGACCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgaca                      <  2:81747/138‑1 (MQ=255)

                                                                                                                    cccggaccagccggaccaCCCGGAGCGCCGGGAGCCCCGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                    <  2:4683963/139‑1 (MQ=255)

                                                                                                                    cccggaccagccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                    <  1:730902/139‑1 (MQ=255)

                                                                                                                    cccggaccagcaggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGCGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                    <  2:3055195/139‑1 (MQ=255)

                                                                                                                      cggggcagccggaccaGCGGGAGCGAGTGGCGCCACTGGAGCTCCCGGCGAAGCAGGACCACCCGGACCCCCCGGAGGGACTGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCggcgcgacaggagcgacaggag                  <  2:4574031/135‑1 (MQ=11)

                                                                                                                          ccagcgggaccaAGGGGTGGGTGGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac              <  1:1093870/140‑1 (MQ=17)

                                                                                                                          ccagccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac              <  2:1433241/140‑1 (MQ=255)

                                                                                                                          ccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac              <  1:4920902/140‑1 (MQ=255)

                                                                                                                            agccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGgagcgacaggagcgacaggagcgacag            <  1:1181728/140‑1 (MQ=255)

                                                                                                                            acccggaccaACCGTCGCGACGGCAGCCACGGGTGCTACCGGGCAAGCCGGACCAGCCGTCCCAACCGCAGCGTCAGGCGCCGCAGGAGCTACGGTGGAAGCGGGACCAGCCGgaccgaccggagcgacaggagcgacag            <  1:612304/138‑1 (MQ=14)

                                                                                                                             gccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg           <  1:2615480/140‑1 (MQ=255)

                                                                                                                             gccggaccaCCCGGAGCGACCGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg           <  2:2414407/140‑1 (MQ=255)

                                                                                                                             gccggaccaCCAGGAGCTACGGGAGCCACGGGAGCTACCGGTGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg           <  2:2362223/140‑1 (MQ=255)

                                                                                                                              ccggaccaCCCGGCGCGACGGGAGCCACGGGAGCTACCGGGGAAGCGGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAgcgacaggagcgacaggagcgacagga          <  1:1437942/140‑1 (MQ=255)

                                                                                                                              ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGTACCACCAGTACCAACCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAACCCGGACCACCAGGcgcgtctggagcgagaggcgcgccaggc          >  2:1869061/1‑139 (MQ=17)

                                                                                                                              ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAgcgacaggagcgacaggagcgacagga          <  1:3101054/140‑1 (MQ=255)

                                                                                                                              ccggaccaACCGGAGCGACGGGAGCCACCGGAGCTACCGGGGAGGCCGGACCAGCAGGACCTACCGGAGCGCCCGGAGCCACATGACCTACCGCCGAAGCCGGAGCAGCCGGagctactggagcgagaggagcgacaggg          >  1:2417103/1‑139 (MQ=17)

                                                                                                                                 ccccaACGTGAGCGACGGGAGCCTCCGGAGCTACCGGGGTAGCCGGACCAGCCGGAGCAACGGGAGCGACTGGAGCGCCTGGAGCTCCCGGGGAAGCCGGACCAGCCGGCGCGACCGGAGCGACAGGAGCGACAGGAGCt       <  2:4409370/138‑1 (MQ=11)

                                                                                                                                      aCCGGAGCGACGGGAGCCACGGGCGCTACCGGGGAAGCCGCACCACCCGGACCAACCGGAGCGACCGGAGCGACAGGTTCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCgg  <  2:567188/140‑1 (MQ=17)

                                                                                                                                      aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACCGGAGCTACCgg  >  2:3569450/1‑140 (MQ=255)

                                                                                                                                      aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCgg  >  2:394001/1‑140 (MQ=255)

CGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607225‑607498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`